Enhanced Production of Cellobiase by Marine Bacterium Cellulophaga lytica LBH-14 from Rice Bran under Optimized Conditions Involved in Dissolved Oxygen

Wa Gao,정정한,Jianghong Li,이진우 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1

The optimal conditions for production ofcellobiase by C. lytica LBH-14 at flask scale had beenpreviously reported. In this study, parameters involved indissolved oxygen in 7 and 100 L bioreactors were optimizedfor pilot-scale production of cellobiase. The optimalagitation speed and aeration rate for cell growth of C. lyticaLBH-14 were 400 rpm and 1.11 vvm in a 7 L bioreactor,whereas those for production of cellobiase were 330 rpmand 0.70 vvm. The analysis of variance (ANOVA) impliedthat significant factor for cell growth was the aeration rate,whereas those for production of cellobiase were theaeration rate as well as the agitation speed. The optimalinner pressures for cell growth and production of cellobiaseby C. lytica LBH-14 in a 100 L bioreactor were 0.00 and0.06 MPa, respectively. The maximal production of cellobiasein a 100 L bioreactor under optimized conditions using ricebran was 140.1 U/mL, which was 1.52 times higher thanthat in a flask scale.

농도분극현상을 이용한 담수화 과정에서의 나노채널 개수 및 운전조건 영향에 대한 실험 적 연구

이용남(Longnan Li),정정열(Jung-Yeul Jung),김대중(Daejoong Kim) 한국가시화정보학회 2015 한국가시화정보학회지 Vol.13 No.2

Concentration polarization(CP) phenomena show great potential on desalination technology. As we can control the dimension of silicon based nanochannel system, it can be used to model ion exchange membrane. In this study, to investigate the effect of nanochannel number and working conditions on the CP based desalination process, nanochannel based microfluidic system is fabricated. First, we optimized nanochannel number and working conditions to conduct visualization study on CP based desalination process. Second, we visualized the desalination process with fluorescent dye in the desalination chip. We also visualized that the particles also can be removed by the CP based desalination process.

Optimization of Mineral Salts in Medium for Enhanced Production of Pullulan by Aureobasidium pullulans HP-2001 Using an Orthogonal Array Method

Wa Gao,Yi-Joon Kim,정정한,Jianhong Li,이진우 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.5

Based on intuitive analyses and statistical calculations using data from orthogonal array experiments,the optimal concentrations of K2HPO4, NaCl, MgSO4·7H2O,and (NH4)2SO4 in cell growth medium of Aureobasidium pullulans HP-2001 were measured as 7.5, 1.0, 0.1, and 2.4g/L, respectively, whereas those for the production of pullulan were 2.5, 0.25, 0.8, and 0.3 g/L, respectively. The most important factor for cell growth and production of pullulan by A. pullulans HP-2001 was identified as K2HPO4. Optimal concentrations of glucose and yeast extract, along with the initial pH of the cell growth medium of A. pullulans HP-2001 containing optimized salt concentrations, were found to be 100.0, 10.0, and 6.0 g/L, respectively, whereas those for the production of pullulan were 100.0, 2.5, and 6.0 g/L, respectively. Conversion rates of pullulan from 10.0, 25.0, 50.0, 75.0, and 100.0 g/L of glucose in the presence of optimized salt concentrations were 26.0, 25.2,22.4, 17.9, and 14.1%, respectively, whereas those in the presence of previously reported salt concentrations were 26.6, 25.2, 19.9, 14.3, and 11.7%, respectively. Optimal salt concentrations for the production of pullulan by A. pullulans HP-2001 varied according to the concentrations of the carbon and nitrogen sources, especially at higher concentrations.

협부 간극에 발생한 활액막 육종: 증례보고

한지훈(Ji-Hoon Han)윤규호(Kyu-Ho Yoon),정정권(Jeong-Kwon Cheong),배정호(Jung-Ho Bae),이설옥( Hsueh-Yu Li),오영일(Young-Il Oh),신재명(Jae-Myung Shin),백지선(Jee-Seon Baik),박관수( Kwan-Soo Park) 대한구강악안면외과학회 2011 대한구강악안면외과학회지 Vol.37 No.6

Synovial sarcoma (SS) is a malignant soft tissue tumor comprising 5-10% of all soft tissue sarcomas. This tumor normally occurs in the paraarticular regions of the extremities but is rare in head and neck sites. SS is sometimes difficult to diagnose because it can mimic benign lesions both clinically and radiologically. This paper presents a rare case of a SS of the buccal space of a 25-year old man. The histology examination and immunohistochemistry of the mass led to a diagnosis of synovial sarcoma. The patient was treated primarily with a surgical resection, followed by radiotherapy and chemotherapy. The follow up examination 17-months after surgery showed no signs of tumor relapse or metastasis.

Pilot-scale Optimization of Parameters Related to Dissolved Oxygen for Mass Production of Pullulan by Aureobasidium pullulans HP-2001

Wa Gao(고와),Yi-Joon Kim(김이준),Chung-Han Chung(정정한),Jianhong Li(이잔홍),Jin-Woo Lee(이진우) 한국생명과학회 2010 생명과학회지 Vol.20 No.10

Aureobasidium pullulans HP-2001 균주를 사용하여 풀루란을 대량 생산을 위하여 7 l 및 100 l 생물배양기를 사용하여 용존산소와 관련된 조건을 최적화하였다. 풀루란의 생산에 최적인 탄소원과 질소원은 각각 50.0 g/l 포도당 및 2.5 g/l 효모추출물이었으며 플라스크 규모에서의 풀루란 변환율은 37%이었다. 풀루란 생산 균주의 생장에 최적인 배지의 초기 pH 및 배양온도는 7.5 및 30℃이었으나 풀루란의 생산에 최적인 배지의 초기 pH 및 배양 온도는 각각 6.0 및 25℃이었다. 7 l 생물배양기에서 Aureobasidium pullulans HP-2001 균주의 생육에 최적인 교반속도 및 통기량은 각각 600 rpm 및 2.0 vvm이었으나 풀루란 생산에 최적인 조건은 각각 500 rpm 및 1.0 vvm이었으며 최적 조건에서 풀루란의 생산농도는 18.13 g/l이었다. 100 l 생물배양기에서 풀루란 생산 균주의 생장에 최적인 내압은 0.0 ㎏f/㎠이었으나, 풀루란 생산에 최적인 내압은 0.4 ㎏f/㎠이었으며 최적 조건에서 풀루란의 생산 농도는 22.89 g/l이었다. 이는 내압이 없는 상태에 비하여 풀루란의 생산 농도가 1.38배 증가한 것이다. Parameters related to dissolved oxygen for the production of pullulan by Aureobasidium pullulans HP-2001 were optimized in 7 l and 100 l bioreactors. The optimal concentrations of glucose and yeast extract for the production of pullulan were 50.0 and 2.5 g/l, respectively, and its conversion rate from glucose was 37% at a flask scale. The optimal initial pH of the medium and temperature for cell growth were 7.5 and 30℃, whereas those for the production of pullulan were 6.0 and 25℃. The optimal agitation speed and aeration rate for cell growth were 600 rpm and 2.0 vvm in a 7 l bioreactor, whereas those for the production of pullulan were 500 rpm and 1.0 vvm. The production of pullulan with an optimized agitation speed of 500 rpm and aeration rate of 1.0 vvm was 18.13 g/l in a 7 l bioreactor. Maximal cell growth occurred without inner pressure, whereas the optimal inner pressure for the production of pullulan was 0.4 ㎏f/㎠ in a 100 l bioreactor. The production of pullulan under optimized conditions in this study was 22.89 g/l in a 100 l bioreactor, which was 1.38 times higher than that without inner pressure.

여정실 발효추출물의 항산화 활성 평가

이정정(Li, Jingjing),전민선(Jeon, Minsun),육홍선(Yook, Hongsong) 한국생활과학회 2022 한국생활과학회 학술대회 Vol.2022 No.5

대학생의 스마트폰 중독 척도 개발 및 타당화

이정정 ( Li Qing-qing ),이정화 ( Lee Jeong-hwa ),김은미 ( Kim Eun-mi ),박병기 ( Bak Byung-gee ) 한국아동교육학회 2021 아동교육 Vol.30 No.1

본 연구의 목적은 스마트폰 중독에 대한 측정도구를 개발하고 타당화하는 것이다. 기존의 스마트폰 중독 측정도구의 문제점을 보완하여 대학생 대상의 스마트폰 중독 척도를 개발하고 이 도구의 타당성을 검증하고자 하였다. 이를 위해 인터넷 중독 척도와 기존의 스마트폰 중독 척도를 참고하여 요인을 설정하고 문항을 구성하였다. 중국의 간쑤성 천수시 T대학과 후베이성 상양시 H대학의 대학생 422명을 대상으로 각각 211명씩 탐색집단과 교차집단으로 나누어 진행되었다. 예비조사와 본조사를 통해 수집된 자료를 분석하여 일상생활 장애, 학업장애, 금단, 내성, 가상 인간관계 지향성의 5요인, 총 25문항을 스마트폰 중독 척도의 최종 문항으로 확정하였다. 본 연구에서 개발한 척도는 일차원성이 확연히 드러났으며 높은 신뢰도를 보여주었다. 척도의 타당도는 내용타당도, 구인타당도, 준거타당도, 교차타당화의 측면에서 다양하게 검토되었다. 내용타당도는 문항개발 및 선정, 예비연구를 통해 확인하였다. 구인타당도는 최종 척도의 측정모형과 법칙론 관계망을 분석함으로써 검토되었다. 준거타당도는 기존의 스마트폰 중독 척도와의 관계를 준거로 판단하였다. 교차타당화는 탐색집단과 교차집단 간에 측정모형의 차이를 분석함으로써 판단하였다. 구인타당도의 경우 스마트폰 중독 척도의 구성요인과 문항간 관계구조의 타당도를 확인하기 위해 확인적 요인분석을 실시한 결과, 절대적합지수 χ2, RMSEA(root mean square error of approximation), 증분적합지수TLI(Tucker-Lewis' index), CFI(comparative fit index) 모두 양호한 적합도를 보였고, 요인부하량을 확인한 결과 문항들은 구성요인을 잘 반영하였다. 법칙론적 연결망 분석의 경우 부정적 양육행동, 학업스트레스, 스마트폰 중독 간 관계를 매개모형으로 설정하여 확인하였다. 매개모형의 적합도 지수 RMSEA는 .093으로 다소 높게 나타났지만, TLI는 .912, CFI는 .936으로 산출되어 양호한 적합도를 보였다. 준거타당도의 경우, 본연구의 새로운 척도가 기존 척도 Su 외(2014)의 척도와 유의한 정적상관을 보임으로서 지지되었다. 교차집단에서 측정모형이 양호한 적합도를 보이고, 탐색집단과의 차이 분석 결과, 두 집단 간에 형태, 측정, 구조가 동일하여 교차타당화가 만족된 것으로 판단되었다. The purpose of this study was to develop a smartphone addiction scale and demonstrate the reliability and validity of the smartphone addiction scale, by improving the problems of the existing smartphone addiction measurement tools, to develop a measurement of smartphone addiction for college students and demonstrate the validity of this tool. For this purpose, the factors were set and questions were organized by referring to the internet addiction scales and the existing smartphone addiction scales. The participants were 422 college students from Chinese Tianshui T University in Gansu and Chinese Xiangyang H University in Hubei. They were divided into an exploratory group and a cross group of 211 college students each. After that, the data collected through the preliminary study and the main survey were analyzed and five sub-factors of obstacles in daily life, obstacles to learning, withdrawal symptom, tolerance, and virtual interpersonal relationship directivity were confirmed. A total of 25 items were confirmed as the final questions on the smartphone addiction scale. As a result of analyzing the scale developed in this study, the one-dimensionality of the items were confirmed and showed high reliability. Then the construct validity, criterion validity and cross validity were estimated to verify the validity of the scale. The content validity was confirmed through the development, selection and preliminary research of the questions. The construct validity was reviewed by analyzing the measurement model and the nomological network of the final scale. The criterion validity was judged to be based on its relationship with the existing smartphone addiction scale. The cross validity was determined by analyzing the differences in the measurement model between the exploratory group and the cross group. As the result of construct validity from the confirmatory factor analysis conducted to verify the validity of the structure of the relationship between the components of the smartphone addiction scale and the questions, the absolute fit index χ2, RMSEA(Root Mean square error of approximation), incremental fit index TLI(Tucker-Lewis' index), and CFI(Comparative Fit Index) all showed a better fit. For the nomological network analysis, the relationship between negative parenting behavior, academic stress, and smartphone addiction is confirmed by setting it as a medium model. The fit index RMSEA for the nomological network model was found to be higher at .093, but the TLI was calculated to be .912, and the CFI to be .936, which showed a better fit. As a result of criterion validity, a new scale in this study was supported by showing significant positive correlation with the existing scale of Su et al. (2014). The measurement model showed a better fit in the cross group, and as the result of the analysis of the differences with the exploratory group, it was confirmed that cross validity was satisfied because the form, measurement, and structure between the two groups were same.

여정실 추출물을 첨가한 양갱의 품질 특성 및 항산화 활성 평가

이정정(Jing-Jing Li),김경희(Kyoung-Hee Kim),육홍선(Hong-Sun Yook) 한국식품영양과학회 2022 한국식품영양과학회지 Vol.51 No.6

본 연구에서는 우수한 생리활성을 가진 여정실 추출물을 앙금 대비 0~8%(2% 간격)를 첨가하여 제조한 양갱의 품질 특성 및 항산화 활성을 분석하고 여정실의 건강식품 소재로서의 이용 가치를 확인하고자 하였다. 양갱의 pH와 당도는 모두 여정실 추출물 첨가량이 증가할수록 유의적으로 낮아졌다(P<0.05). 수분 함량은 여정실 추출물 첨가량이 증가할수록 유의적으로 증가하였다(P<0.05). 양갱의 명도 L값은 대조군이 가장 낮았으며 여정실 추출물을 첨가할수록 낮아지는 경향을 보였다. 반면에 적색도 a값과 황색도 b값은 모두 첨가군이 대조군보다 높게 나타났으며 여정실 추출물 첨가량이 증가할수록 높아지는 경향을 보였다. 기계적 조직감 측정 결과 경도는 대조군이 가장 높았고, 여정실 추출물 첨가량이 많을수록 감소하여 8% 첨가군이 가장 낮은 값을 보였다. 부착성, 탄력성은 대조군과 여정실 추출물 첨가군 사이에 유의적인 차이가 없었다. 여정실 양갱의 항산화 활성을 측정한 결과, 총 폴리페놀 및 플라보노이드 함량은 여정실 추출물 첨가량이 증가함에 따라 증가하였다(P<0.05). 또한, DPPH 및 ABTS 라디칼 소거능, 환원력(FRAP)의 활성은 모두 여정실 추출물 첨가량이 증가함에 따라 유의적으로 증가하여 8% 첨가군에서 가장 높게 나타났다(P<0.05). 이상의 연구 결과를 바탕으로 여정실 추출물 8%를 첨가하여 제조된 양갱은 건강 양갱으로서 충분히 가치가 있으며 우수한 항산화 활성을 가진 여정실은 건강기능성 식품 소재로써 활용 가능성이 있을 것으로 판단된다. This study investigated methods to increase the availability and value of Fructus Ligustri Lucidi to be used as a healthy functional food material. The quality characteristics and antioxidant activity of Yanggaeng with different percentages (0%, 2%, 4%, 6%, 8% w/v) of Fructus Ligustri Lucidi extract were analyzed. The pH and sweetness of Yanggaeng decreased with the addition of Fructus Ligustri Lucidi extract (P<0.05). The moisture content of Yanggaeng increased with an increase in the proportion of Fructus Ligustri Lucidi extract (P<0.05). An analysis of color values showed that the lightness (L) of Yanggaeng decreased with increasing amounts of Fructus Ligustri Lucidi extract, but the redness (a) and yellowness (b) increased (P<0.05). The texture profile analysis showed that the hardness increased with the increasing content of Fructus Ligustri Lucidi extract (P<0.05), but the adhesiveness and springiness showed no significant differences between the control and experimental groups (P<0.05). Upon testing the antioxidant activity of Fructus Ligustri Lucidi with Yanggaeng, the total content of polyphenols and flavonoids increased as the amount of Fructus Ligustri Lucidi extract increased. In addition, the DPPH, ABTS free radical scavenging activities, and ferric reducing antioxidant power were the lowest in the control group and significantly increased as the Fructus Ligustri Lucidi extract content increased (P<0.05). Based on these results, Yanggaeng with an 8% content of Fructus Ligustri Lucidi extract shows promise as a health food. Fructus Ligustri Lucidi thus has the potential to be used as a healthy functional food material that also exhibits antioxidant activity.

발효 여정실의 건강기능성 식품 및 화장품 소재로써 사용 가능성 평가

이정정(Jing-Jing Li),김경희(Kyoung-Hee Kim),육홍선(Hong-Sun Yook) 한국식품영양과학회 2023 한국식품영양과학회지 Vol.52 No.7

본 연구에서는 여정실을 여러 유용 미생물 B. subtilis KCTC 14549(BS), S. cerevisiae KCTC 48234(SC), L. brevis KCTC 18270(LB), L. casei KCTC 12413(LC), L. plantarum KCTC 18510(LP)으로 발효한 후 항균, 항산화 및 효소 저해 활성을 측정하여 발효 여정실의 건강 기능성 및 화장품 등과 같은 산업원료 소재로서 활용성을 탐색하였다. 여정실 발효액의 pH, OD600nm, 생균수 측정 결과는 각각 3.45~4.43, 0.73~1.21, 5.20~8.75 log CFU/mL의 범위를 나타내서 발효가 잘 진행되었음을 확인하였다. 여정실 발효물의 항산화 활성(총 페놀 함량, DPPH 라디칼 소거능 및 FRAP)을 측정한 결과, 모두 SC를 이용한 발효 여정실의 항산화 활성이 유의적으로 가장 높게 나타내었다. 항균 활성을 측정한 결과, LP 발효균이 P. aeruginosa에 아주 뛰어난 항균 활성을 나타내었다. 여정실 tyrosinase 저해 활성을 측정한 결과, 모두 발효군이 비발효군보다 높게 나타났으며 특히 LB, LP, SC 발효군이 비교적 높았다. 여정실 발효물의 elastase 및 collagenase 저해 활성은 SC 발효군이 가장 높게 나타내었다. 본 연구 결과, 여정실 발효를 통해 항산화, 항균 및 효소 저해 활성을 증진할 수 있으며, 특히 SC를 이용한 발효 여정실의 항산화 및 효소 저해 활성 강화, LP를 이용한 발효 여정실의 항균 활성 증대를 확인하여 발효 여정실이 건강기능성 식품 및 화장품 원료 소재 등으로 활용할 수 있음을 제시하고 있다. In this study, Fructus Ligustri Lucidi was fermented with B. subtilis, S. cerevisiae (SC), L. brevis (LB), L. casei (LC), and L. plantarum (LP). To evaluate the role of the resultant fermentation products as raw materials in functional health foods and cosmetics, the antioxidant, antibacterial, and enzyme inhibitory activities were measured. The pH, 600 nm absorbance, and live cell count of the fermentation products in the fermentation room were in the range of 3.45∼4.43, 0.73∼1.21, and 5.20∼8.75 log CFU/mL, respectively, confirming that the fermentation had proceeded well. The antioxidant activity based on the total phenol content, 2,2-diphenyl-1-picrylhydrazyl radical scavenging ability and ferric reducing antioxidant power assay, was highest in the fermentation product associated with SC (P<0.05). The fermentation product associated with LP showed excellent antibacterial activity against P. aeruginosa. All the fermented products showed higher tyrosinase inhibitory activity than the non-fermented product, and this was especially true of the LB, LP, and SC fermented products. The elastase and collagenase inhibitory activities were observed to be the highest in the SC fermentation product. The results of this study confirm that antioxidant and enzyme inhibitory activities can be enhanced through Fructus Ligustri Lucidi fermentation with S. cerevisiae. Also, L. plantarum fermentation produced a product with highest antibacterial activity. This suggests the possibility of using the fermented products of Fructus Ligustri Lucidi as raw material for the manufacture of functional health foods and cosmetics.

Study on subway ridership estimation with the consideration of endogeneity

Tingting Li(이정정),Jungyeol Hong(홍정열),Dongjoo Park(박동주) 한국철도학회 2017 한국철도학회 학술발표대회논문집 Vol.2017 No.05

본 연구는 지하철 이용자 수요예측을 위하여 내·외생적 영향요인들을 분석하고, 각 요인들이 일반선형회귀모형 상에서 내재하고 있는 편의를 통제하여 보다 설명력이 높은 예측 방법론을 제시하는데 주요 목적이 있다. 지하철 이용수요와 관련하여 기존 연구들은 주로 사회경제 및 토지이용 등의 요인들과의 상관관계 위주의 연구를 수행해 왔으나, 버스 등의 타 대중교통 수요의 내생성 및 지하철역 주변상업용도의 외생적 영향에 대한 연구는 고려하지 않았다. 따라서 본 연구는 이러한 문제들을 개선하기 위하여 2단계 최소자승법(2SLS)을 적용하였으며, 연구결과를 통하여 버스 이용자수와 상업용도 요인들의 내·외생성은 2SLS 추정에 의해 지하철 이용수요 예측을 보다 고도화 할 수 있는 것으로 나타났다. The objective of this study is to develop a consistent model for predicting subway ridership. Previous studies have focused on the relationship between subway ridership and factors such as socioeconomy and land-use. However, there was a lack of research on the endogenous impacts of alternative transit demand and exogenous trade area. Therefore, this study incorporated the effects of bus ridership and retail properties within 300m radius from 248 subway stations in Seoul in the modeling process. In a methodological aspect, we applied two-stage least squares (2SLS) approach to capturing the endogeneity issue. As a result, bus ridership and attributes for trade area were found to be significant, and the methodological approach for the model development was plausible to estimate subway ridership.