개머루덩굴 추출물의 자극에 의한 피부장벽 개선 효과

박선경(Seon Gyeong Bak),임형진(Hyung Jin Lim),원영선(Yeong-Seon Won),박은재(Eun Jae Park),오제훈(Je Hun Oh),김지은(Ji Eun Kim),이민지(Min Jee Lee),이소영(Soyoung Lee),이승웅(Seung Woong Lee),이승재(Seung Jae Lee),노문철(Mun Chual Rho 한국축산식품학회 2023 Food and Life Vol.2023 No.1

The skin is the largest organ that blocks invasion to the outside. Due to external stimuli, the skin barrier collapses and immune function abnormalities occur. This leads to skin diseases such as atopic dermatitis and psoriasis. Through this study, we attempted to develop a natural material that can be used for various dermatitis diseases. In this study, the gene expression of skin barrier-related proteins was confirmed using tumor necrosis factor-α/interferon-γ-stimulated HaCaT cells through real-time PCR. As a result, it was confirmed that the gene expression of skin barrier-related proteins and ceramide-related proteins was improved by the ABE-M. In this study, it was confirmed that the gene expression of filaggrin, involucrin, and loricrin, which are skin barrier-related proteins, was improved by ABE-M, and it was confirmed that the gene expression of the tight junction-related proteins, occludin and zonula occludens proteins-1, was also increased. As such, it is expected that ABE-M can be used as a material for skin inflammation such as atopic dermatitis.

백내장수술 후 발생한 급성 안내염의 발생률과 위험인자 분석

이승재(Seung Jae Lee),김지원(Jiwon Kim),홍정화(Jung Hwa Hong),정은지(Eun Jee Chung) 대한검안학회 2020 Annals of optometry and contact lens Vol.19 No.4

Purpose: To evaluate the incidence and risk factors of acute postoperative endophthalmitis (POE) after cataract surgery from 2011 to 2015 in Korea. Methods: A nationwide retrospective cohort study was performed by using claims data from the Korean National Health Insurance Service (KNHIS) database collected during 2011 to 2015. Cataract surgery and acute POE were identified using Korean Electronic Data Interchange (KEDI) code and Korean Classification of Disease (KCD)-7 code. Results: A total of 2,236,107 eyes in 1,591,176 patients (60.16% female; mean age 69.37 ± 10.07 years) underwent cataract surgery from 2011 to 2015 in Korea. The incidence of acute POE after cataract surgery was 0.09% and remained stable during 5 years period as the unadjusted incidence rate ratio (IRR) was 1.025 (95% confidence interval 0.988-1.064; p = 0.1932). A total of 2,041 eyes experienced acute POE (51.23% female; mean age 67.84 ± 11.99 years) after 8.46 ± 10.18 days after the cataract surgery. In multivariate analysis, intraoperative posterior capsule rupture, male gender and preoperative diabetes were associated with a higher risk of acute POE: 5.643 (4.454-7.148, p < 0.001), 1.408 (1.283-1.544, p < 0.001), 1.241 (1.125-1.371, p = 0.0167), respectively. Living in rural area, low household income and surgery in tertiary healthcare centers were socioeconomic factors significantly associated with higher risk of acute POE. Conclusions: The incidence of acute POE after cataract surgery in Korea remained unchanged during 5-year study period. Intraoperative posterior capsule rupture, male, preoperative diabetes, low household income and surgery in tertiary healthcare centers were associated with higher risk.

강박장애 환자의 공감능력 손상 및 그와 연관된 강박증상차원

김솔지(Sol Jee Kim),이상원(Sang Won Lee),김지은(Ji Eun Kim),이승재(Seung Jae Lee) 대한생물치료정신의학회 2014 생물치료정신의학 Vol.20 No.3

Objectives:Patients with obsessive-compulsive disorder(OCD) suffer from serious impairments in social functioning. However, OC and comorbid depressive symptoms only partially account for these impairments. Therefore, this study aimed to investigate basic empathic abilities and their relationships with OC symptom dimensions in patients with OCD. Methods:Forty two patients with OCD and 99 healthy graduate students completed the Interpersonal Reactivity Index to measure four dimensions of empathy including perspective taking(PT), fantasy(FS), empathic concern(EC), and personal distress(PD). The Yale-Brown Obsessive Compulsive Scale(Y-BOCS), the Y-BOCS Symptom Checklist, and the Beck Depression Inventory were also measured. Results:We found that patients with OCD scored lower on PT(F=42.2, p<.001), and EC(F=4.9, p=.03) subscales, and higher on PD subscale(F=31.7, p<.001) than normal controls. However, after adjusting for comorbid depression and education, only PT subscale remained significant(F=12.4, p=.001). Regarding the associations between empathy components and OC symptom dimensions within the patient group, cleaning dimension solely showed positive correlations with PT(r=0.36, p=.019) and FS(r=0.51, p=.001) scores. Conclusion:These findings suggest that, irrespective of their OC or depressive symptoms, patients with OCD may have impairment in perspective taking ability, and washers are likely to show more intact empathic abilities than patients with other OC dimensions.

프리캠버가 적용된 7.5m 무동바리 장스팬 거푸집의 구조성능에 관한 실험적 연구

강지수 ( Ji Su Kang ),이승재 ( Seung Jee Lee ),송종욱 ( Jong Wook Song ),원용호 ( Yong Ho Won ),최성모 ( Sung Mo Choi ) 한국복합신소재구조학회 2016 복합신소재구조학회논문집 Vol.7 No.2

Cap decks used in slim floor system for reducing floor height act as molds for forming floor slabs in structures with a span of up to 6m without temporary supports. When deep decks are employed in a structure with a span of more than 6m, the problem of defection occurs. This study suggests placing tendons at the ends of cap decks in order to create cambers and control defection when preload is applied. Uniformly distributed load was applied by an UTM to the two L/4 points of specimens to observe the deflection of preloaded cap decks. For the test, three 7.5m specimens were fabricated. One of them was a plain cap deck specimen and the others were those with tendons (PC strands or steel bars.

생체모방형 바이오리액터를 위한 배양시스템 개발

김소연(So-Youn Kim),지민희(Min-Hee Jee),정훈진(Hoon Jin Jeong),이인환(In-Hwan Lee),이승재(Seung-Jae Lee) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12

Recent experimental researches support the hypothesis that mechanical stimuli play a role in regulating specialized molecules expression in articular cartilage in vitro and in vivo. Therefore we are studying bioreactor to provide a stimulus of mimicked real knee environment. Our bioreactor system consists of a joint motion stimulation device and culture chamber. We fabricated a stimulation system similar to movement of the CPM device for human joint motion. This system is composed of a linear stage, ball-spline and links. Also we fabricated a culture system for media circulation and mimicked knee environment. However, previous culture system has some problems that media leakage and interference of joint model. In this study, we improved a new-chamber system for non-interference of joint model and medium sealing. Then, this system could be used to investigate the effect of dynamic culture.

3D 프린팅으로 제작된 무릎 관절모델의 압축력 측정

정훈진(Hoon Jin Jeong),지민희(Min-Hee Jee),김소연(So-Youn Kim),이승재(Seung-Jae Lee) 한국기계가공학회 2014 한국기계가공학회지 Vol.13 No.2

Recent experimental observations support the hypothesis that mechanical stimuli play a role in regulating the specialized molecular expression of articular cartilage in vitro and in vivo. Other studies have demonstrated that the continuous passive motion(CPM)bioreactor for whole joints can provide a platform for possible future in vitro studies and applications, including possible interactions of bio-mechanical and biochemical signals. In this study, we have developed acustom-made bioreactor capable of bending and stretching with circular type motion, and a biomimetic knee joint model, using a 3D printer. This system could be used to investigate the effects of rehabilitative joint motion of dynamic culture.

체외수정시술시 복부 초음파 유도하 배아이식의 연령에 따른 임상적 효용성

김동원 ( Dong Won Kim ),김석현 ( Seok Hyun Kim ),민응기 ( Eung Gi Min ),이승재 ( Seung Jae Lee ),이정렬 ( Jung Ryeol Lee ),최영식 ( Young Sik Choi ),지병철 ( Byung Chul Jee ),구승엽 ( Seung Yup Ku ),서창석 ( Chang Suk Suh ),최영민 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.3

Objective: To evaluate the clinical efficacy of transabdominal ultrasound-guided embryo transfer for in vitro fertilization and embryo transfer (IVF-ET) according to age of women. Methods: A total of 211 cycles of IVF-ET were included in this study. The pregnancy outcomes were compared between transabdominal ultrasound-guided embryo transfer (USG-guided group, n=108) and clinical touch technique (Clinical touch group, n=103) groups. Then, comparative analysis was performed in women aged <35 years, 35~37 years and in those ≥38 years. Results: Clinical characteristics of study subjects did not differ between the two groups. There was no difference between the two groups in terms of duration of controlled ovarian hyperstimulation, dose of gonadotropins used, numbers of oocytes retrieved and embryos transferred. Clinical pregnancy rate was significantly higher in USG-guided group than Clinical touch group (27.8% vs. 16.5%, p<0.05). This difference was also found in women 35~37 years (27.6% vs. 8.6%, p<0.05), but not in those <35 years. USG-guided group showed a significantly higher implantation rate in women 35~37 years (10.5% vs. 3.4%, p<0.05), but not in those <35 years. In women at 38 years or older, USG-guided group showed higher clinical pregnancy and implantation rates than Clinical touch group (15.0% vs. 7.6%; 4.8% vs. 2.5%, respectively), however the differences were not statistically significant. Conclusion: Transabdominal ultrasound-guided embryo transfer may improve the pregnancy outcomes in IVF-ET cycles, especially in women at age 35 years or older.

Ampicillin과 Furosemide의 相互作用에 관한 硏究

李昇宰,鄭址昌,高啓昌,韓大燮 慶熙大學校 1986 論文集 Vol.15 No.-

In order to investigate the durg-interaction between furosemide and ampicillin, the influences of ampicillin on the diuretic action of furosemide and the effects of furosemide on the urinary elimination rate and serum concentration of ampicillin were studied in the rabbits. In comparison of diuretic effects of furosemide alone group with those of furosemide-ampicillin combined group, the furosemide-ampicillin combined group showed significant increase in urine flow and urinary electrolytes amounts. Those findings seem to be resulted chiefly from marked inhibition of sodium and chloride reabsorption in the renal tubule without a little alteration of glomerular filtration rate. Throughout the combined administration of furosemide and ampicillin. the serum concentration of ampicillin were significantly elevated, but the urinary elimination rate of ampicillin were markedly decreased in the observed rabbits. It is concluded from the results that synergistic effect of ampicillin on diuretic action of furosemide might be due to the competitive inhibition of plasma protein binding with furosemide by ampicillin. It is also postulated that the competition between furosemide and ampicillin in renal tubular secretion.

학동전 정상 소아에서 연령별 혈청 총 IgE 집먼지진드기 특이 IgE 및 ECP 치에 관한 전국적 조사 연구

이하백,박규창,양승,김용주,오재원,문수지,나영호,안영민,최재경,김광우,이승재,한동기,박진녕 대한 소아알레르기 및 호흡기학회 1999 소아알레르기 및 호흡기학회지 Vol.9 No.2

목 적 : 혈청 총 IgE와 알레르겐 특이 IgE는 알레르기 질환의 진단을 위한 초기검사 방법의 하나로서 널리 이용되고 있으며, 전 세계적으로 가장 주요한 알레르겐인 집먼지진드기(Dermatophagoides pteronyssinus : Dp, D.farinae :Df)는 조기 노출 및 감작에 의하여 천식, 비염 및 아토피 피부염의 발병 가능성을 높여 주고 있기 때문에 자주 검사의 대상이 되고 있다. 한편, 알레르기 염증에 관여하는 호산구의 생산물인 eosinophil cationic protein(ECP)는 기관지 상피세포 손상, 기관지 염종 및 기관지 과민반응을 유발하는 것으로 밝혀진 후 혈청내 ECP 측정의 임상 응용이 활발하다. 본 연구는 학동기전 정상 소아에서 혈청 총 IgE 및 집먼지진드기 특이 IgE 및 ECP의 국내 정상참고치를 제시함으로써 알레르기질환의 진단에 필요한 기본적인 자료를 설정하는데 도움이 되고자 하였다. 방 법 : 1996년 5월부터 1997년 8월까지 서울, 경기, 강원, 경북, 전남 등 진국 5개 지역에서 무작위로 선정되고 그 지역을 대표할 수 있는 9개 병원의 육아상담실에 내원한 그리고 보육기관에서 거주하면서 발열이나 상기도 감염 및 알레르기질환의 과거력과 가족력이 없는 7세 미만의 건강한 소아 449명을 대상으로 하였다. 이들로 부터 각각 말초 정맥혈을 채취 후, 원심분리하여 -20℃에서 냉동 보관 후 혈청 총 IgE, Dp-lgE, Df-IgE 및 ECP치를 fluoroimmunoassay(UniCAP, Pharmacia-Upjohn, Sweden)로 측정하고, 그 각각의 결과에서 기하평균과 신뢰구간을 구하여 분석 평가하였다. 결 과 : 1) 연령별 혈청 충 IgE는 출생 후 4새까지 서서히 증가하다가 이후에 완만한 커브를 나타내었다. 2) 혈청 집먼지진드기 특이 IgE(Dp-IgE와 Df-IgE) 치는 3세 이후 특히 남아에서 나이가 들면서 학동기까지 각각 서서히 의미있게 증가하였다. 3) 혈청 ECP치는 출생 후 4세까지 점차적으로 증가한 후 감소하는 추세를 보였다. 4) 혈청 총 IgE, Dp-IgE와 Df-IgE 및 ECP치는 남아와 여아 사이에 통계학적으로 의미있는 차이는 없었다. 5) 혈청 총 IgE, Dp-IgE와 Df-IgE 및 ECP치의 지역별 차이를 보면 Dp-IgE와 Df-IgE가 도시 지역에서 해안에 가까운 기타 도시 지역보다 다소 높았으나, 통계학적으로 유의한 차이는 없었다. 6) 혈청 총 IgE, Dp-IgE와 Df-IgE 및 ECP치 사이의 상관성을 보면 총 IgE와 Dp-IgE(r=0.321, P<0.001) 및 Df-IgE(r=0.324, P<0.001) 그리고 Dp-IgE와 Df-IgE(r=0.943, P<0.001) 사이에 각각 양의 상관관계가 있었으나, ECP치와는 유의한 상관성이 없었다. 결 론 : 혈청 총 IgE 및 ECP치는 4세 전후에 그리고 집먼지진드기 특이 IgE는 3세 이후에 증가를 시작하는 것으로 미루어 조기 감작의 예방이 필요하며, 본 연구 결과는 학동전 소아의 정상 참고치로서 이용 가능할 것으로 사료된다. Purpuse : Serum IgE have been shown to be related to allergic disease and used for an initial diagnosis of allergic diseases. House dust mite such as Dermatophagoides pteronyssinus(Dp) and D. farinae(Df) is very important for inhalant allergens, which may be a cause and/or triggering factor of atopic diseases. On the other hand, eosinophil cationic protein (ECP) causes epithelial damages of the airway, and bronchial hyperresponsiveness has been used as a useful indication of allergic inflammation. To date, there are few studies on the longitudinal patterns of total and inhalant allergen-specific IgE, and ECP in normal children in Korea. The present study was performed to determine the reference levels of serum total and Dp- and Df-specific IgE and ECP in healthy children under 7 years of age by the groups of different age and gender. Methods : Total, and Dp- and Df-specific IgE and ECP concentrations were measured by fluoroimmunoassay(UniCAP, Pharmacia-UpJohn, Sweden) in serum from 449 healthy children under 7 years of age by using a population-based cohort followed from 1996 through 1997. All subjects were enrolled from eight large health organizations in geographically defined area such as Seoul and four local cities. Results : Serum total IgE concentration showed steady increase with age from birth to 4 years, and a plateau. In contrast, Dp- and Df-IgE levels showed apparently continuous increments after 4 years of age. ECP showed variable increase until 4 years of age and slow decrease afterwards. There were no statistically significant differences in those results by gender and/or the living area, but much higher increments of Dp- and Df-IgE of boys from 3 years of age through 6 years were found. Conclusion : We found feasible results of total, Dp- and Df-specific IgE, and ECP in serum from healthy children under seven years of age, which may be useful for a reference.