Tandem Promoters for Expression of Cnu, a Nucleoid Protein, in Escherichia coli

이설훈,김원경,최지수,장성희,임헌만,이영훈 대한화학회 2019 Bulletin of the Korean Chemical Society Vol.40 No.8

P1, P2, and P3, three tandem promoters of cnu.

파지디스플레이를 이용한 성장인자 안정화 제형 맞춤형 피부 투과 펩타이드의 개발

이설훈 한국미생물·생명공학회 2018 한국미생물·생명공학회지 Vol.46 No.4

본 연구에서는 성장인자, 효소, 펩타이드 등과 같은 기능성 생체 고분자를 대상으로 열에 대한 안정성 및 피부 투과성을 향상시키는 연구를 수행하였다. 이들은 생체 내에서 세포를 활성화 하거나 촉매 작용을 담당하고 있다. 따라서 화장품 등의 외용제에 적용 시, 그 효능의 우수함이 예상되나열에 대한 불안정성과 높은 분자량으로 피부 투과성이 낮은단점이 있다. 이를 극복하기 위해 먼저 열에 대한 안정성을확보할 수 있는 조성을 탐색하였다. 그 결과, 단분자 구조의humectant 대비 PEG의 길이가 긴 polymeric humectant를사용한 경우 열에 대한 안정성이 높아지는 것을 확인 할 수있었다. 한편 이들의 피부 투과를 촉진시키기 위하여 투과 촉진 펩타이드를 phage library로부터 선별하고자 하였다. 투과 촉진 펩타이드는 성장인자, 효소, 펩타이드의 투과 촉진을 위해 공통적으로 사용할 수 있는 구조이다. 그러나 피부의 투과정도는 물질자체의 특성도 영향을 미칠 수 있으나 제형의성분에 따라서 영향을 받을 수 있다. 본 연구에서는 성장인자를 안정화 할 수 있는 polymeric humectant 제형을 기반으로 투과 촉진 펩타이드 선별을 수행하였다. 그 결과 대조군 펩타이드 대비 투과촉진이 향상된 결과를 확인했을 뿐만아니라 PBS를 기반으로 선별된 투과 촉진 펩타이드 보다polymeric humectant 제형에서는 투과도가 우수한 것을 확인 할 수 있었다. 본 연구의 결과는 기능성 생체고분자의 열 안정성 개선 및피부 투과도 향상에 기여할 수 있을 것으로 기대된다. In this study, we identified methods to improve heat stability and skin permeability of functional protein biopolymers, such as growth factors, enzymes, and peptides. The biopolymers participate in cellular activation and catalytic functions in vivo. Therefore, when applied to cosmetics, their efficacies are expected to be helpful for skin care. However, they have disadvantages that include instability to heat and low skin permeability due to their high molecular weight. To overcome these problems, we searched for a composition that increases heat stability. Stability was improved using a polymeric humectant having a long polyethylene glycol length, compared with a mono-molecular structure humectant. Next, to enhance skin permeation, a permeation enhancing peptide was selected from a phage library. The permeation enhancing peptide can be commonly used to promote the permeation of growth factors, enzymes, and peptides. Screening was performed on the polymeric humectant formulation. One dominant peptide from the modified- screening method was identified. Furthermore, it was confirmed that the permeability of the peptide was better than that of the peptide developed through a screening system based on phosphate-buffered saline. The data indicate that the polymeric humectant formulation will be helpful for increasing the heat stability of protein ingredients and that skin permeability could be increased by a formulation-specific, penetration-enhancing peptide.

파지 디스플레이를 이용한 피부 투과 기능성 펩타이드의 개발

이설훈,강내규,이상화 대한약학회 2013 약학회지 Vol.57 No.2

Biologically active peptides, including growth factors and cytokines, participate in various biological processes in human skin. They could provide a great advantage of maintaining healthy skin. Many peptide growth factors like epi- dermal growth factor (EGF) and human growth hormone (hGH) have been used in cosmetic formulations. The delivery of peptide growth factors across the Stratum corneum, however, seems not sufficient because of their physical properties such as high molecular weight and hydrophilicity. So increasing the penetration of growth factors of interest into skin would be a major concern for ensuring their maximum biological efficacy. In this study, we have identified several skin penetration- enhancing peptides which facilitate delivery of growth factors, when fused at N-terminus of the target protein, into skin. For efficient and rapid screening, we constructed a skin-penetrating assay system using Franz cell and porcine skin. Next, we carried out phage display screening using M-13 bacteriophage with random 12 –amino acid library on its coat protein P3 on that system. After several selection rounds, peptide sequences facilitate the penetration of phages through the porcine skin were identified from a large population of phages. We found that phages with the most potent peptide (S3-2, NGSLNTHLAPIL) could penetrate the porcine skin eight times more than those with control peptide (12 mino acids scram- bled peptide). Furthermore, growth factors conjugated with S3-2 peptide penetrate porcine skin three to five times effi- ciently than non-conjugated growth factors. In conclusion, our data shows that the skin penetration-enhancing peptide we have characterized could increase the delivery of growth factors and is useful for cosmeceutical application.

An Importance-performance Analysis to Selection Attributes of Chinese Theme Parks: the Case of Fragrant Hills and Happy Valley

설훈구 동북아시아문화학회 2012 동북아 문화연구 Vol.1 No.33

Successful marketing of a theme park requires an understanding of its positioning in the market in today's competitive environment. Marketers are keenly interested in the concept of selection attributes as it relates to travel decision-making. Research in the selection attribute forming in a theme park typically considers the importance and the perceived performance. If research in selection attribute formation is to produce results managerially meaningful, it needs to simultaneously consider the why issue for the gap between the importance and perception. Attitudes are composed of both subjective and objective assessments of attributes that a destination possesses. Adding the objective dimension to subjective performance gives practitioners more strategic options and helps them to understand the visitors’ image formation process. Theme parks are fantasy settings in which both child and adult are actively immersed into fantasy environments inspired by literature, films, television, and so on. Theming is reflected through architecture, landscaping, costumed personnel, rides, shows, food services, and other services that influence the guest experience (Milman, 2010). The number of theme parks is increasing, and the quality of them is thriving in China (China Business Focus, 2009). Most theme park development in China has concentrated on the major populated area around the Guangzhou, Shanghai, and Beijing-Tianjin areas (Lew, et al., 2003). The purpose of this study is to determine the relative importance of factors tourists rely on in their evaluations of a theme park and their assessments of theme parks in China. This research looks to find out visitor’s perceptions to the importance and the performance, or satisfaction in selection attributes between these two different theme parks in location and theme. One is dedicated to a large-scale of natural and historical theme parks in Beijing-Tianjin area. The other is themed as entertainment in Shen Zhen. This study can be utilized as a certain positioning for these two competing theme parks.

말뚝-지반 상호작용을 고려한 수정된 하중전이함수법 제안

설훈일(Seol Hoon-Il),정상섬(Jeong Sang-Seom),김영호(Kim Young-Ho) 대한토목학회 2008 대한토목학회논문집 C Vol.28 No.6

말뚝의 하중전이거동 및 변형특성 해석을 위해 하중전이함수법이 널리 사용된다. 본 연구에서는 말뚝-지반의 상효작용 즉, 지반의 연속성을 고려한 하중전이해석에 고찰하였으며, Mindlin 해를 이용하여 이를 고려함으로써 말뚝의 실제 거동에 보다 부합되도록 수정된 하중전이 해석방법을 제안하였다. 이를 통해 말뚝지반 상호작용의 영향인자인 말뚝직경-지반계수의 비(D/Es)과 주면마찰력-재하하중의 비(Rs/Q)를 고려할 수 있었다. 제안된 하중전이함수법의 타당성을 검증하기 위하여 현장재하시험 사례와의 비교분석 결과, 제안된 해석방법은 암반 근입 현장타설말뚝의 하중침하 거동 및 하중전이특성을 적절히 예측함을 알 수 있었다. The load distribution and deformation of pile subjected to axial loads are evaluated by a load-transfer method. The emphasis is on quantifying the effect of coupled soil resistance that is closely related to the ratio of pile diameter to soil modulus (D/Es) and the ratio of total shaft resistance against total applied load (Rs/Q), in rock-socketed drilled shafts using the coupled load-transfer method. The proposed analytical method that takes into account the soil coupling effect was developed using a modified Mindlin's point load solution. Through comparisons with field case studies, it was found that the proposed method in the present study estimated reasonable load transfer behavior of pile and coupling effects due to the transfer of shaft shear loading, and thus represents a significant improvement in the prediction of load deflections of drilled shafts.

패밀리ㆍ패스트푸드 레스토랑의 선택 속성을 통한 시장세분화에 관한 연구

설훈구(Sul Hoon-Ku),강성욱(Kang Sung-Wook),박기용(Park Ki-Yong) 한국관광레저학회 2007 관광레저연구 Vol.19 No.4

21C를 준비하는 대학의 과제

설훈,Seol, Hun 한국대학교육협의회 2000 大學敎育 Vol.107 No.-

제주도 방문 베트남 관광객의 개인가치와 관광지 선택속성만족이 재방문의도에 미치는 영향

설훈구(Sul, Hoon-Ku) 동북아시아문화학회 2018 동북아시아문화학회 국제학술대회 발표자료집 Vol.2018 No.10

유한요소해석을 통한 암반에 근입된 현장타설말뚝의하중전이거동 분석

설훈일,김영호,정상섬 한국지반공학회 2008 한국지반공학회논문집 Vol.24 No.12

The load distribution and deformation of rock-socketed drilled shafts subjected to axial loads are evaluated by a load-transfer method. The emphasis is on quantifying the effect of coupled soil resistance in rock-socketed drilled shafts using the 2D elasto-plastic finite element analysis. Slippage and shear load transfer behavior at the pile-soil interface are investigated by using a user-subroutine interface model (FRIC). It is shown that the coupled soil resistance provides the influence of pile toe settlement as the shaft resistance is increased to an ultimate limit state. The results show that the coupling effect is closely related to the value of pile diameter over rock mass modulus (D/Emass) and the ratio of total shaft resistance against total applied load (Rs/Q). Through comparisons with field case studies, the 2D numerical analysis reseanably presented load transfer of pile and coupling effect due to the transfer of shaft shear loading, and thus represents a significant improvement in the prediction of load deflections of drilled shafts.

외식 가맹 사업의 영향력, 만족, 갈등과 장기지향성에 관한 연구

설훈구(Sul Hoon-Ku),정희정(Jung Hee-Jung) 한국관광레저학회 2009 관광레저연구 Vol.21 No.2