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이상오,이철기,윤일수,Lee, Sang-O,Lee, Choul-Ki,Yun, Ilsoo 한국ITS학회 2022 한국ITS학회논문지 Vol.21 No.5
The thesis is automated traffic control equipment system aims to improve. Areas where improvement is needed about the existing automated traffic control equipment by applying the latest technology and to improve the things that can be derived. Reflecting the derived improvements, we intend to present a plan for the design and implementation of a new unmanned traffic control device. The main improvements were designed to change the housing material of the unmanned traffic control equipment, simplify the configuration of the equipment, reduce the weight of the equipment, and change the purpose of the software. In order to evaluate the objective performance of the improved unmanned traffic control equipment through this study, it was requested to a public certification authority. The reliability of the equipment was secured through KC certification and durability test. It is intended to present the feasibility of securing the marketability of the unmanned traffic control equipment by comparing and evaluating the construction period and installation cost with the existing unmanned traffic control equipment.
Methylotrophic Yeast, Pichia pastoris에서 사람 락토페린의 발현 및 항균성 연구
이상오,임은미,남은주,이현환,Lee Sang O,Im Eun Mi,Nam Eun Joo,Lee Hyune Hwan 한국미생물학회 2004 미생물학회지 Vol.40 No.4
사람의 모유에 많이 함유된 human lactoferrin(hLF)은 항균 및 항 바이러스 작용이 있는 것으로 보고되고 있다. 본 연구에서는 hLf를 메탄올자화 효모인 Pichia pastoris에 cloning하고 그 발현을 RT-PCR, Northern blotting, SDS-PAGE및 Western blotting으로 확인하였다. 그 결과 2.1 kb의 hLf 유전자가 P.pastoris의 염색체 DNA로 끼어들어가 안정적으로 hLf를 발현하였다. 이 재조합 P.pastotis로부터 hLf를 포함하는 세포 추출액을 얻어 항균 작용을 연구하였다. 발현된 재조합 hLf는 Staphylococcus aureus, Micrococcus flavus 등의 그람 양성균에 대해 강력한 항균작용을 보일 뿐만 아니라 그람 음성 동물성 병원균인 Pseudomonas fluorescens ID 9631, E. coli ATCC8739, 25922,35 등과 Salmonella typhimurium 114,115 등 다양한 균에 대해서도 강력한 항균작용을 보였다. 이는 재조합 hLf가 생물학적 활성이 있다는 것을 보여준다. The expression and antibacterial. activity of recombinant human lactoferrin (hLf) was studied from methylotrophic yeast Pichia pastoris. The gene encoding hLf, isolated from human breast cDNA library, was subcloned into the expression vector, pPIC3.5K under the control of AOX1 promoter. The gene was integrated into the host chromosome and was identified by Southern blotting. The expression of the integrated gene was investigated by RT-PCR, Northern blotting, SDS-PAGE and Western blotting. Discrete band corresponding to hLf was detected from the SDS-PAGE, which was confirmed by Western blotting. The expression was also confirmed by RT-PCR and Northern blotting. The antibacterial activity of the recombinant hLf (rhLf) was investigated using Staphylococcus aureus ATCC 6538P and Micrococcus flavus ATCC 10240 as test organisms. The rhLf showed strong antibacterial activities against the bacteria. Furthermore, many Gram-negative animal pathogens such as E.coli ATCC8739, 25922, and Salmonella typhimurium 114 and 115, Pseudomonas fluorescens ID 963 I, P. aeruginosa KCCM 11802, and Gram-positive bacteria Bacillus mesentericus were also inhibited in their growth by the rhLf.
지하철 객실 냉난방 서비스품질에 영향을 미치는 요인분석 및 시사점(9호선 사례를 중심으로)
이상오(Sang Oh Lee),박정수(Jung Su Park) 한국철도학회 2013 한국철도학회 학술발표대회논문집 Vol.2013 No.5
본 연구에서는 도시철도의 서비스품질 중 주요한 요소인 전동차 객실 냉난방 민원인자들을 파악하고 요인분석 및 예측을 통하여 열차이용 고객의 만족을 극대화하고자 한다. 객실 냉난방민원발생에 원인이 되는 변수들을 나열하여 이에 냉난방 민원건수에 미치는 상관성을 몇 가지 분석을 통해 연구하고자 하였다. 연구의 목적을 달성하기 위하여 본 연구에서는 9호선 사례를 통하여 개통 초기부터 지속적으로 제기되고 있는 냉난방민원방생 건수를 요인분석에 의한 결과값을 인용해 그 중 상대적으로 다수 민원발생건수가 증가하는 환절기 일일 민원발생인자요소들을 나열하여 최종 로직스틱 회귀분석을 도출 예측하여 도시철도 냉난방민원발생건수 감축방안에 틀을 제시하고자 하였다. This article is to maximize customer satisfaction by analyzing cause and expectation of problems after classifying factors to service quality from too cold or hot in the cabin during the visit. And it tries to research correlation between complaints from too cold or hot in the cabin and factors by regression analysis To complete the aim, it presents improvement to cut down on number of complaints from temperature in the cabin in the changes of season specially, based on line 9 case, to have been increased continuously since the initiation of line 9, by frequency analyzed and resulted data from cause and logistic regression analysis.
이상오 ( Sang-oh Lee ) 대한영어영문학회 1996 영어영문학연구 Vol.20 No.1
The aim of this paper is to review some principles accounting for the relative scope ambiguity of the quantificational expressions in double object constructions in English. For this purpose, various scope principles based on the theory of quantifier raising and the theory without recourse to quantifier raising will be discussed. Taken together Minimalist assumptions, the approach without recourse to quantifier raising functions to handle the relative ambiguity problems of the quantificational expressions in double object constructions in English, coupled with the A-chain theory.