인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

난청인을 위한 전화기 음성증폭 및 하울링 억제

이상민,Lee, Sang-Min 대한의용생체공학회 1998 의공학회지 Vol.19 No.6

일반전화기로 통화가 어려운 난청인에게 충분한 수신음량을 공급하기 위해서는 큰 증폭이 필요한데 큰 증폭은 하울링(howling)을 발생시킨다. 본 연구에서는 소리를 크게 증폭하면서 하울링을 억제하는 새로운 방법을 제안하고 이를 적용한 보청전화기를 개발하여 평가하였다. 통화수신 음성은 3개의 밴드로 나뉘어져 각각 독립적으로 증폭되어 난청인의 청력에 알맞게 적합(fitting)된다. 수신음성의 주파수는 카운터에 의하여 시간영역에서 모니터링되는데 카운터부는 비교기를 이용하여 수신 정현파를 구형파로 변환시키고 시간영역에서 구형파 수를 계수함으로써 주파수를 분석하여 하울링이 발행하는가를 판단한다. 전화기는 송수화기 구조가 결정되어 있고 송수신 주파수 범위가 제한되어 있으므로 하울링 또한 일정주파수 범위내 발생하게 된다. 계수기에 의해 하울링이 검출되면 중앙제어부는 해당대역의 증폭도를 빠르게 낮춤으로써 하울링을 억제시킨다. 시험결과 개발전화기는 하울링 없이 40dB 이상 증폭할 수 있으며 중고도 난청인의 어음인지력이 1음절어의 경우는 20%에서 60.8%로, 2음절어의 경우는 28.9%에서 78%로 향상되는 탁월한 개선 효과를 보였다. To provide sufficient sound to the hearing-impaired person(HIP) who have many difficulty in communication with others using general telephone, big amplification is needed. But big amplification can occur howling as a side effect. In this study we developed the new technique of big amplification without howling, manufactured and estimated the new hearing aid telephone. Telephone speech is divided to three frequency band, amplified respectively and fitted to HIP's hearing ability. The telephone speech frequency is monitored by counter in time domain. The counter transfers the sinusoidal sound to rectangular wave using comparator and counts the number of rectangular wave in a certain time period, that is frequency, to monitor the howling. Telephone have microphone and speaker, which are fitted in a rigid structure and frequency band of telephone sound is limited, so howling occurs in the limited frequency band. If the counter notices that howling conditions happen, microprocessor decreases quickly the gain of the related frequency band. The result of test of our new hearing aid telephone showed that we can amplifiy the sound as much as 40dB, which is meaningful level to many HIP, and make HIP increase their perception ability from 20% to 60.8% in 1 syllable test and from 28.9% to 78% in 2 syllable test.

학교시설 통합정보시스템 구축방안에 관한 연구 - 정보화 구축설계 방향을 중심으로 -

이상민,노근철,유승호,Lee, Sang Min,Ro, Keun Chul,Yoo, Seung Ho 한국교육녹색환경연구원 2017 교육·녹색환경연구 Vol.16 No.1

학교 시설이 가장 중요한 공공시설 중 하나라는 사실에도 불구하고 학교시설에 대한 정보가 체계적으로 관리되지 않고 있는 실정이다. 이에 본 연구에서는 학교시설 정책의 가장 기본적인 요소인 학교시설 현황에 대한 객관적인 데이터를 체계적으로 확보하기 위한 시스템 구축방향에 대한 연구를 진행하였다. 본 연구에서는 학교 시설 정보 관리 절차 및 학교시설의 정보 자료 관리 수준을 향상시킬 수있는 시스템 구축방향을 제안하였으며, 실무적인 활용이 가능한 서비스 제공을 위한 시스템 구축 설계방향을 제시하였다. Despite the fact that school facilities are one of the most important public facilities, information about school facilities is not systematically managed. The purpose of this study was to investigate the direction of system construction to provide objective data on school facilities, which is the basic element of school facility policy. In this study, we propose a system construction method to improve the information management procedure of school facility information and school facilities, and suggest a system design direction for providing service that can be practically used.

경두개 자기 자극이 인지 기능에 미치는 영향

이상민,채정호,Lee, Sang Min,Chae, Jeong-Ho 대한생물정신의학회 2016 생물정신의학 Vol.23 No.3

Transcranial magnetic stimulation (TMS) is a safe, noninvasive and useful technique for exploring brain function. Especially, for the study of cognition, the technique can modulate a cognitive performance if the targeted area is engaged, because TMS has an effect on cortical network. The effect of TMS can vary depending on the frequency, intensity, and timing of stimulation. In this paper, we review the studies with TMS targeting various regions for evaluation of cognitive function. Cognitive functions, such as attention, working memory, semantic decision, discrimination and social cognition can be improved or deteriorated according to TMS stimulation protocols. Furthermore, potential therapeutic applications of TMS, including therapy in a variety of illness and research into cortical localization, are discussed.

녹색건축 전문가 자격제도 개발 연구

이상민,이승민,Lee, Sang Min,Lee, Seung Min 한국교육녹색환경연구원 2014 교육·녹색환경연구 Vol.13 No.1

In an effort to resolve environmental pollution problems, developed countries enforce green building certification systems. The same is true in Korea. However compared professional accreditation system to certification systems did not systematically in Korea. Therefore, in accordance with development of green building related systems and technology the requirement for foster green building professionals is urgent. Accordingly this study is purposed to analyze and propose that green building professional accreditation system development and operation of the direction. Through data analysis domestic and international green building professional accreditation system for draw a development plan. And then conduct an expert Delphi survey reflected in the survey results to development plan.

『미군정자료연구』 (정용욱 저, 도서출판 선인, 2003)

이상민,Lee, Sang-Min 한국기록학회 2003 기록학연구 Vol.0 No.7

비구형 정상상태 형상 주위의 비점성 액적 진동

이상민,강인석 ( Sang Min Lee,In Seok Kang ) 한국화학공학회 1996 화학공학의이론과응용 Vol.2 No.2

아키비스트 윤리 규약과 논평

이상민,Lee, Sang-Min 한국기록학회 2003 기록학연구 Vol.0 No.7

시간 지연과 시변 상관성 제거 필터를 이용한 디지털보청기용 궤환제거 알고리즘

이상민,박영철,정세영,김인영,김선일,Lee, Sang-Min,Park, Young,Jung, Se-Young,Kim, In-Young,Kim, Sun-I 대한전자공학회 2005 電子工學會論文誌-SC (System and control) Vol.42 No.4

In digital hearing aid system, one of the main problems is acoustic feedback which is known as howling because of miniaturization md high-gain amplification. In this paper, we proposed a feedback cancellation algorithm for hearing aid using time delay and time-varying decorrelation filter. The proposed algorithm has a kind of adaptive filter structure, which is combined with time delay and time-varying decorrelation filter to improve feedback cancellation. An all pass filter was implemented as the time-varying decorrelation filter using low frequency modulator. From the result of computer simulation, it is verified that the proposed algorithm has good ability to cancel feedback. 디지털 보청기 시스템에서는 소형화, 고이득 증폭이 요구되는데 이로 인한 문제점 중의 하나가 하울링(howling)이라는 음향의 궤환이다. 본 논문에서는 시간지연과 시변 상관성 제거 필터를 이용하여 보청기에서 사용 가능한 음향 궤환 제거 알고리즘을 제안하였다. 제안한 알고리즘은 적응형 필터 구조를 기본으로 음향 궤환 신호 제거 효과를 증대시키기 위해 시간 지연과 상관성 제거 필터를 삽입하였다. 상관성 제거 필터로 사용된 전대역 통과 필터는 저주파 변조기를 이용하여 시변 특성을 갖도록 구현 하였다. 컴퓨터 시뮬레이션 결과를 통하여 제안한 알고리즘의 궤환 제거 특성이 우수함을 확인하였다.

사료 첨가제로서 한약제가 참전복 치패의 성장 및 체성분에 미치는 영향

이상민,박철수,김동수,LEE Sang-Min,PARK Chul Soo,KIM Dong Soo 한국수산과학회 2001 한국수산과학회지 Vol.34 No.5

우리나라의 주 양식 대상종인 참전복의 성장이나 육질을 개선시킬 수 있는 배합사료의 첨가제로서 한약제인 어보산의 효능을 평가하기 위해 대조사료와 한약제 첨가사료로 3회의 사육실험을 실시하였다. 평균체중 206mg, 827mg 및 432rng의 참전복을 대상으로 사료당 3반복으로 각각 10주간 (실험 1), 7주간 (실험 2) 및 19주간 (실험 3) 사육 실험한 결과, 모든 실험에서 생존율은 차이가 없었다 (P>0.05). 모든 실험에서 Obosan-C 첨가구의 체중 및 각장 성장은 대조구와 유의한 차이를 보이지 않았지만, 평균체중 432mg의 치패을 대상으로 19주간 사육 실험 3의 경우에는 Obosan-O 첨가 실험구의 체중 및 각장 성장이 대조구보다 유의하게 높았다 (P<0.05). 실험 종료시 전복 가식부의 수분, 단백질 및 회분 함량은 각 실험에서 한약제 첨가구와 대조구간에 차이가 없었으나, 실험 2에서 전복 가식부의 찌질 함량은 Obosan-C 첨가구가 대조구보다 유의하게 낮은 갔을 보였다 (P<0.05). 이상의 결과로부터 배합사료에 Obosan-O 첨가는 참전복의 성장을 개선시키는데 도움이 될 것으로 기대된다. Three feeding experiments were conducted to investigate the effects of herbs (Obosan-C or Obosan-O) as an additive in formulated diets on growth and body composition of juvenile abalone (Haliotis discus hannai), Three replicate groups of abalone initially average weighing 206 mg, 827 mg and 432 mg were fed the each one of experimental diet containing $0\%\;or\;1\%$ Obosan for 10 (experiment 1), 7 (experiment 2) and 19 weeks (experiment 3), respectively. In addition, an imported commercial diet for abalone was also employed to compare growth performance of juvenile abalone. Weight gain of abalone fed the diet containing Obosan-O was significantly (P<0.05) higher than that of abalone fed the control diet in the experiment 3. Moisture, crude protein and ash contents of soft whole body were not influenced by dietary herbs (P>0.05), whereas crude lipid content of abalone fed the diets containing Obosan-C was significantly (P<0.05) lower than that of abalone fed the control diet in the experiment 2. The results indicate that Obosan-O as an additive in formulated diet can improve body growth of juvenile abalone.