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원경미,김진우,조미영,박명애,지보영,명정인 한국수산과학회 2013 Fisheries and Aquatic Sciences Vol.16 No.2
In 2009, a systemic megalocytivirus infection associated with high mortality was detected for the first time in cultured starry flounder Platichthys stellatus in Korea. Diseased starry flounder had pale bodies and gill coloring and enlarged spleens. Histopathological examinations revealed basophilic enlarged cells in various organs of diseased starry flounder. Polymerase chain reaction (PCR) was performed on tissue samples using three published primer sets developed for the red sea bream iridovirus. PCR products were detected for all primer sets, except 1-F/1-R, which are registered by the World Organization for Animal Health (OIE). The part of the gene corresponding to the full open reading frame encoding the viral major capsid protein (MCP) was amplified by PCR. PCR products of approximately 1,581 bp were cloned, and the nucleotide sequences were analyzed phylogenetically. The MCP gene of the starry flounder iridovirus, designated SFIV0909, was identical to that of the turbot reddish body iridovirus (AB166788).
A Discourse Analysis of Students’ Reflections in a Critical Literacy Picturebook Read-Aloud Class
원경미 한국영미문학교육학회 2017 영미문학교육 Vol.21 No.1
This study is based on a read-aloud class picture book focusing on achieving critical awareness. It was conducted over the course of one month at an elementary school in Korea and analyzed how sixth-grade students responded to texts which raised questions about multiple perspectives, gender roles, and the status of marginalized minorities. The research was conducted on twenty sixth-graders in an elementary school in Gyeonggi-do. The read-aloud class was organized based on Four Resources Model of Reading and consisted of two sessions. During the first two-week session, students read a text on the basis of code-breaking and meaning-making practices, and during the second session that was conducted in the last two weeks, they read the texts through the prism of text-using and text-analyzing practices. In order to investigate whether critical literacy instruction developed critical awareness in the students, the students’ discourses in their written reflections on the texts were analyzed using Fariclough’s Three-Dimensional Model of Discourse Analysis. The results indicated that the read-aloud critical literacy class enhanced students’ engagement with the texts by enabling them to question the issues that were in play. In addition, six out of the twenty students demonstrated their critical awareness in their reflections. They produced critical literacy-oriented discourses including perspective-challenging discourses that questioned the authors’ points of view, individual-difference discourses, and positive-discrimination discourses, rather than conventional, conservative discourses. Therefore, the results of this study suggest that it would serve elementory school instructors well to employ critical literacy practices in their reading classes. However, further research is required in order to gain more information regarding the applicability of critical literacy instruction in the EFL context. The results of the study and their pedagogical implications will be discussed in detail in this paper.
β-1,3/6-linked glucan 첨가사료가 넙치의 비특이적 면역반응에 미치는 영향
원경미,김수미,박수일 한국어병학회 2004 한국어병학회지 Vol.17 No.1
glucan 첨가 사료 투여가 넙치에 미치는 영향을 조사하기 위하여 넙치에 glucan 0%, 0.01%, 0.1%, 0.5% 첨가 사료를 연속 투여구와 격주 투여구로 나누어 공급하면서, 성장, 혈액 성분, 비특이적 면역능을 분석하였으며, 나아가 어병 세균인 Edwardsiella tarda균의 공격 실험에 대한 방어능을 조사하였다. gulcan 첨가 사료 투여는 넙치의 성장에 긍정적인 효과를 보였는데, 특히 0.05% 연속 투여구와 0.1% 격주 투여구에서 대조구보다 유의하게 높은 성장을 나타내었다. glucan 첨가사료의 투여가 넙치의 비특이적 면역 반응에 미치는 영향을 조사하기 위해 혈청 lysozyme과 전신 식세포의 확성 산소를 측정한 결과, 거의 모든 실험구에서 실험 기간 내내 대조구보다 높은 활성을 보였다. glucan 첨가 사료의 투여가 넙치의 생리에 영향을 미치는 지를 조사하기 위하여, total protein, glucose, GOT, GPT, Ht를 측정한 결과, 3주째 GPT 수치가 다소 높은 것을 제외하고는 그다지 큰 변화를 보이지 않았다. 마지막으로 Edwardsiella tarda (4×10^(6) cells/fish) 균을 공격 실험한 결과, 0.01% 연속 투여구를 제외한 모든 실험구에서 높은 방어력을 보였다. The effects of dietary β-1,3/1,6-linked glucan administration on nonspecific immune responses, hematology and disease resistance against Edwardsiella tarda in olive flounder, Paralichthys olivaceus were evaluated. Fish were fed the experimental diets supplemented with 0 %, 0.01 %, 0.05 %, 0.1 %, and 0.5 % of β-glucan to a commercial diet for 7 weeks. The test was performed in two ways such as fed on each β-glucan concentrations daily and every other week alternately. The body weight gain from the fish fed on daily the 0.05 % supplemented diet and fed on alternately the 0.1 % supplemented diet of β-glucan were significantly higher than the control. Both lysozyme activity and intracellular superoxide anion production of kidney phagocytes were higher in the all experimental groups than in the control. But there was no large difference in hematology among each group. The relative percent survival rate (RPS) after an artificial challenge with 4×10^(6) cells of E. tarda per fish was higher than the control, except for that fed on daily the 0.01 % supplemented diet.