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오성종,양보석,김희석,이근상,김강식,스피어스,아우리 ( S . J . Oh,B . S . Yang,H . S . Kim,K . S . Lee,K . S . Kim,J . Spears,H . M . Oury ) 한국축산학회 1986 한국축산학회지 Vol.28 No.7
This study was conducted to investigate the effects of breed and parity of recipients, grade of frozen-thawed embryos and degree of donor-recipients synchrony on conception rate when the frozen-thawed bovine embryos were transferred to 60 heads of Korean Native cattle (KNC), Holstein and crossbred in Livestock Experiment Station, Rural Development Administration, and nearby large farms. To synchronize the estrus, regularly cycling heifers and cows of 114 heads were injected with 50㎍ of cloprosternol twice 11 days apart. The results obtained from this study were summarized as follows; 1. Cows showed estrus with the range of 2-6 days after the first shot and 2.5-4 days after the second shot. The rate of response was higher in the second shot (82.5%) than the first shot (67.5%). 2. The rate of response to cloprostenol after the first shot was high in crossbred, Holstein and KNC in the order, although no significant differences were noted in the second shot. 3. The overall conception rate was 45.0% and that of Holstein was 52.2% (24/46) and that of KNC was 37.5% (3/8). However none of crossbred was pregnant. 4. The conception rate of heifers (46.9%) was slightly higher than that (42.9%) of cows. 5. The conception rate of cattle transferred with excellent, good and fair embryos were 68.9%, 35.9% and 40.9%, respectively. 6. The conception rate was highest when the recipients began to show estrus 12 hours later than donor did.
오성종,임경순,이용빈 ( S . J . Oh,K . S . Im,Y . B . Lee ) 한국축산학회 1983 한국축산학회지 Vol.25 No.4
This study was carried out to investigate the cloning of mouse and rat embryos. Three-hundred females of DDY and ICR strain mice and fifty females of Wistar strain rats were superovulated by intramuscle injection of PMS and HCG. Two-, four-, and eight-cell embryos were dichotomized in vitro by fine glass, needle with hand following the papaya protease digestion of zona pellucida. The results obtained were summarized as follows; 1. The mice were superovulated by injections of 5 IU of PMS and HCG. One, two, and four to eight-cell embryos were mostly obtained from the oviduct at 18 to 24, 48 and 72 hours after injection of HCG. In rat, at 48 hours after HCG, two-cell embryo was 66%, but at 72 hours, four-cell embryo, 44% and eight-cell embryo, 40%, respectively. 2. The percentage of mouse and rat showed superovulation after PMS and HCG treatment was 44 and 30%, respectively. The average number of embryos ovulated per head was 13.7 ± 4.6 and 25.9 ± 8.3 in mouse and rat. There was highly significant differences in the ovulation response and the number of ova ovulated per head between breeds. 3. The rate of dichotomized two-cell embryo in control, zona softened and removed was 0, 48 and 95% in mouse and 0, 44 and 58% in rat, respectively. Rate of the embryos dichotomized without damaged blastomere was 40 and 93% in zona softened and removed, respectively. 4. The rate of dichotomized four-cell mouse embryo was 0, 64 and 96% in control, zona softened and removed and the rate of embryo dichotomized with out damaged blastomeres was 36 and 46%, respectively. The rate of dichotomized four-cell rat embryo in control, zona softened and removed was also 0, 56 and 63%, respectively. The dichotomized rate of embryo was lower in rat than that in mouse. 5. The dichotomized rate of 8-cell mouse embryos was 60 and 82% in zona softened and removed and showed lower dichotomized rate than that of two- and four-cell embryos. In eight-cell mouse embryo, the rate of embryo dichotomized with out damaged blastomees was 17 and 34% in zona softened and removed.
PCR 기법을 이용한 소 Y- 염색체 특이적 DNA 단편 증폭에 의한 성판별 연구
오성종(S . J . Oh),이창수(C . S . Lee),양보석(B . S . Yang),정진관(J . K . Jung),성환후(H . H . Seong),임경순(K . S . Im) 한국축산학회 1993 한국축산학회지 Vol.35 No.5
This experiment was conducted to indentify the sex of cattle using PCR. Bovine Y-specific and bovine specific sequences as primer were synthesised and genomic DNA was extracted from blood of Korean Native cattle and Holstein. When the bovine specific sequences were added to the reaction buffer as primer, both male and female genomic DNAs were amplified .and formed bands(2166p) after the electrophoresis. However, when the Y-specific sequences were added, only male genomic DNA was amplified and formed bands(141hp). PCR products did not increase when the amount of bovine specific DNA as primer was more than 50ng. Two distinct DNA fragments were detected when amplified by two-step PCR method(that is, amplification with bovine specific DNA for 30 cycles after the ampilfication with Y-specific DNA for 30 cycles).
김희석,오성종,양보석,이근상,정길생 ( H . S . Kim,S . J . Oh,B . S . Yang,K . S . Lee,K . S . Chung ) 한국축산학회 1985 한국축산학회지 Vol.27 No.4
These experiments were conducted to investigate the effects of hormone treatment, serum total cholesterol (T-CHO) levels and flushing site on ova recovery and transfer method on pregnancy rate in cattle. The best number of ova recovered among PMSG treatments was showed in PMSG 3000IU + PGF₂α 25㎎ and the number of ova recovered and recovery rate of FSH were more than those of PMSG. In serum T-CHO levels, the case of more than 150㎎/100㎖ in superovulated cattle showed higher than those of below 150㎎/100㎖ in ova recovery. The recovery rate of uterine body was slightly better than that of each uterine horn. Cleavage stage of ova recovered on 7 to 8 days following estrus were mostly morulae and blastocysts and the average normal rate of ova recovered was 78.7%. The mean length of the cycle preceeding PMSG and FSH treatment were 52.38±20.90 and 23.83±13.26 days, respectively. When PGF₂α was administrated after flushing, the day of interval from the induced estrus to spontaneous estrus tended to shorten. The pregnancy rate in single, A1 + single and twin was 22.0, 35.7 and 35.6%, respectively.
소에 있어서 이식 수정란의 생존성에 영향을 미치는 요인에 관한 연구
김희석,오성종,양보석,유승환,김종국,이근상 ( H . S . Kim,S . J . Oh,B . S . Yang,S . H . Yoo,J . G . Kim,K . S . Lee ) 한국축산학회 1986 한국축산학회지 Vol.28 No.9
This experiment was conducted to investigate the effects of the number of embryos transferred, state (fresh or frozen), developmental stage of embryos and donor-recipient synchrony on pregnancy rate and embryonic loss in cattle. The animals used in this experiment were 95 heads of Korean native cattle, Holstein and crossbred. The results obtained in this experiment are as follows; 1. Fourty six out of 95 recipients (48.4%) were pregnant and embryo mortality rate (EMR) was 23.2%. 2. The pregnancy rate was higher when an embryo was transferred additionally on day 7after the AI on estrus (59.1%) than when one (45.6%) or two (40.0%) embryos were transferred. However, EMR was also higher when an embryo transferred additionally than the other groups. 3. The pregnancy rate of cows transferred fresh embryo was higher (56.0% vs 45.7%) and EMR of fresh embryo transferred cows was lower (20.0% vs 24.3%) compared with those of cows transferred frozen-thawed embryo. 4. The pregnancy rate was slightly higher (50.0% vs 48.1%) when the embryo of blastocyst stage was transferred than when the embryo of morals stage. However, EMR was slightly lower in the cows transferred morals stage embryo (22.8% vs 25.0%). 5. The pregnancy rate when the donors showed estrus 12 hours earlier than the recipients (76.9%) was higher than when the donors and recipients showed estrus at the same time (45.5%) or when the donors showed estrus 12 hours later than the recipients (37.5%). Similar trends were also noted in EMR.
체외수정 및 미세조작에 의한 가축배의 생산과 효율적 이용에 관한 연구 6. 소 핵이식배의 체외배양 , 동결보존 및 성판정에 관한 연구
정영채,김창근,윤종택,나광빈,오성종,이종완,김흥률,김광식,박선애,유영아 ( Y . C . Chung,C . K . Kim,J . T . Yoon,G . B . Luo,S . J . Oh,J . W . Lee,H . R . Kim,K . S . Kim,S . A . Park,Y . A . You ) 한국축산학회 1997 한국축산학회지 Vol.39 No.3
This study was carried out to establish an effective method for the improvement of subsequent development after nuclear transfer(NT) in bovine embryo, for the extension of utility through freezing and sexing of NT embryos. The rates of cleaved and developed embryos upto morula-blastocyst stages after the NT in in vitro produced embryos were 60.6 and 3.7%, respectively. Whereas, in in vivo embryos, slightly higher developments were obtained(70.8 and 5.6%), respectively. No difference was found in embryonic development upto morulablastocyst stages among the different levels of EGF added to the serum containing medium. The rates of cleaved and developed embryos upto morula-blastocyst were higher when electrostimulation was performed either before fusion or after addition at 21h IVM. The survival rate of frozen NT embryos was higher in late morula than that of earlier embryos. PCR-based sexing of NT embryo demonstrated that higher proportion of embryos was male(1.9:1), instead of normal ratio(1:1).
이용빈,오봉국,서국성,오성종,임경순 ( Yong B . Lee,Bong K . Ohh,Guk S . Suh,Sung J . Oh,Kyong S . Im ) 한국축산학회 1979 한국축산학회지 Vol.21 No.1
This study was conducted to investigate the sex ratio between the natural born pigs and B-body test sperm, which the data were obtained from a total of 1.242 pigs born at Swine Farm, Jeil Sugar Co. And it was attempted the sedimentary and galvanic separation. of X-and Y-bearing spermatozoa with 4 boar semens at College of Agriculture, Seoul National University. The results obtained were as follows; 1. The natural sex ratio of pigs born was about 50%, and B-body test sperm was a-so about 50%. It was, therefore, statistically non-significant differences of the sex ratio between the natural born pigs and B-body test sperm. 2. The B-body test sperm obtained from Fractionated semen was similar to B-body sperm of control 3. Spermatozoa of swine were separated into different fractions by sedimentation. B-body rest sperm to anode was 53.70%, and the B-body from bottom was 33.43%. 4. The swine semen could separate X- and Y-bearing sperm by galvanization without interfering the sperm liability. In galvanization of sperm, the percentage of the B-body sperm induced to anode was 60.4%, while the sperm to cathode was 28.15%. This experiments with seminal galvanization and sedimentation for the separation of X- and Y-bearing sperm have been exclusively conducted with swine. Since the data presented in this report were still unstable, more detail research would, so far, be needed.