돼지의 체외수정

박수봉,박항균,입곡명 ( S . B . Park,H . K . Park,Akira Iritani ) 한국축산학회 1990 한국축산학회지 Vol.32 No.1

The present study was conducted to investigate the optimum conditions of in vitro fertilization by ejaculated boar spermatiozoa capacitated in a defined medium. When fresh ejaculated boar spermatozoa were preincubated for 4h with elevated sperm concentration fertilization rates were markedly increased. The fertilization rates by spermatozoa preincubated at the concentrations of 2, 4, 10, 20 and 40×10^8 cells/ml were 12, 30, 50, 76 and 82%, respectively, However, when spermatozoa preserved for 10-12h at 20℃ were preincubated for 4h with elevated sperm concentration fertilization rates were markedly decreased. The fertilization rates by spermatozoa preincubated at the concentrations of 2, 4, 10, 20 and 40×10^8 cells/ml at preincubation were 91, 77, 68, 44 and 8%, respectively. When oocytes matured in vitro were inseminated with spermatozoa at the concentration 1 × I0^6 cells/ml, rates of fertilization and polyspermy were 97 and 87%, respectively, after culture for 20h without changing medium. When oocytes were inseminated with spermatozoa at the different concentrations of 1, 0.5, 0.25 and 0.1×10^6 cells /ml and the medium was changed 5h after insemination, the fertilization rates were 75, 73, 61 and 30%, respectively. The rates of polyspermic fertilization were 58, 47, 40 and 47%, respectively, when oocytes were inseminated with different concentrations of spermatozoa, and there was no difference in the rates of polyspermy fertilization among groups with changing medium. The present results suggested that both sperm preincubation with high concentration and sperm preservation at 20℃ for 10-12h were effective for capacitation ejaculated boar spermatozoa and that a complete block of polyspermy was not achieved in this in vitro fertilization conditions.

학습시간 단축을 위한 역전파 학습 알고리즘 개선

박수봉,박철웅 東新大學校 1997 論文集 Vol.9 No.-

Multilayer neural networks are widely used in classification and recognition due to their ability to learn a range of complicated weights and map. These neural networks are studied and compared with gradient descent for learning with an arbitrary number of hidden units. In this paper, we propose a convergence speed improvement of back- propagation learning algorithm that is used to recognize license plates in vehicles. The proposed algorithm modifies weights by each learning conditions that is decided by (-) sign and (+) sign in weights between the hidden layer and the output layer. First, weights is modified by general back-propagation. And weights among each layers is redundantly modified by weights sign. Through the simulation results to recognize license plates in vehicles, we show that the learning algorithm is faster than BEP(Back-error propagation) in convergence speed. Therefore the learning algorithm is widely applied to image and speech recognition.

CIE X_(10) Y_(10) Z_(10)색표시계에 따른 선글라스용 렌즈의 측색

박수봉,마기중 金泉大學 1992 논문집 Vol.11 No.1

시감투과율이 82%이상으로 한국규격에서 선글라스용 렌즈로 구분될 수 없는 23개 렌즈를 제외한 22개 렌즈에 대해서 CIE X_(10) Y_(10) Z_(10)색표시계에 따른 주파장과 자극순도를 조사한 결과는 다음과 같다. 1. 각기 다른 염료로 착색된 렌즈의 주파장은 각각 갈색 : 582-596nm(CIE A). 575-590nm(CIE B). 572-587nm(CIE D_(65)), 핑크색 : 592-595nm(CIE A). 589-592nm(CIE B). 588-591nm(CIE D_(65)), 청색 : 481-485nm(CIE A). 475-479nm(CIE B). 474-477nm(CIE D_(65), 녹색 : 556-564nm(CIE A). 550-556nm(CIE B). 547-553nm(CIE D_(65))이었다. 2. 자극순도가 25% 이상으로 KS규격을 초과한 렌즈의 염료별 착색시간은 각각 갈색 :30초 이상 (CIE A & D_(65)) 1분이상 (CIE B), 핑크색 : 2분 이상 (CIE A), 5분이상 (CIE D65 & 8), 청색 : 10분 이상 (CIE D_(65)), 20분 이상(CIE B), 녹색 : 20분 이상(CIE A, D_(65) & B)이었다. 한편 평균주광 (D_(65)) 의 color shifts 에 대한 ANSI규격을 초과한 렌즈는 5분 이상 갈색 염료로 착색된 렌즈와 20분 동안 착색된 핑크색 렌즈뿐이었다. The transmittance properties of 45 dyed CR-39 lenses were examined to determine whether these lenses met the KS P-4404 Lenses for Sunglass standards for excitation purity, and colorimetered according to the CIE 1964 supplementary standard colorimetric system. Approximately 50% of the sampled dyed lenses failed to meet KS requirement for luminous transmittance. Of 22 lenses that passed to KS requirement for luminous transmittance. only 11 lenses that met KS requirement for exitation purity.

흔들의자 외 1편

박수봉 시와세계 2022 시와세계 Vol.- No.80

다중 신경망을 이용한 영상 분류기에 관한 연구

박수봉,박종안,Park, Soo-Bong,Park, Jong-An 한국음향학회 1995 韓國音響學會誌 Vol.14 No.1

본 논문에서는 신경망 학습에 의한 영상분류 알고리즘을 개선하였으며, 이것은 입력패턴 생성부와 분류을 위한 역전파 알고리즘에 의한 광역신경망으로 구성된다. 입력패턴을 위한 특징값으로는 자기조직화 형상지도 학습에 의해 얻은 코드북 데이타를 특징벡터로 이용한다. 이것은 입력벡터로서 원영상에 충실하면서 입력 뉴런수를 감소시킨다. 분류기에 사용된 광역망 알고리즘은 가중치와 유니트 오프셋 제어가 가능하도록 역전파 알고리즘에 제어부와 어드레스 메모리부를 삽입하였다. 실험결과 이들 분류기는 학습시 국소최소점에 빠지지 않게 되며, 대규모 신경망을 구현하고자 할 때 망구조를 간단히 할 수 있다. 또한 이것은 동작속도를 크게 개선할 수 있다. In this paper, we improve an image classifier algorithm based on neural network learning. It consists of two steps. The first is input pattern generation and the second, the global neural network implementation using an improved back-propagation algorithm. The feature vector for pattern recognition consists of the codebook data obtained from self-organization feature map learning. It decreases the input neuron number as well as the computational cost. The global neural network algorithm which is used in classifier inserts a control part and an address memory part to the back-propagation algorithm to control weights and unit-offsets. The simulation results show that it does not fall into the local minima and can implement easily the large-scale neural network. And it decreases largely the learning time.

소난포란의 체외성숙과 유전공학적 응용

박수봉 中央大學校 遺傳工學硏究所 1989 遺傳工學硏究論集 Vol.2 No.1

The development of system for maturation of follicular oocyte in vitro is necessary to acquire competent oocyte and early embryos in plentiful number and at a price for studies of the many new technologies. Bovine follicular oocytes matured in vilro achieved fertilization and sudsequently cleavage, but the frequencies of both preimplantation development to blastocyst stage and live young after transfer were low compared to the oocytes matured in vivo. Hence, it has been concluded that a deficient cytoplasmic maturation occures in spontaneously maturing oocytes even though nuclear maturation appear nomal. Probably, the abnormality of spontaneously matured oocytes may have been the result of deficiency in culture system. Also, this problem may be due to unhealthy oocytes recovered from ovaries at random stages of the estrus cycle. This seems likely since hitological studies show that the majority of vesicular follicles on bovine ovaries are in some stages of atresia. These factors indicate that the studies for maturation of bovine follicular oocyte in vilro need improvement of culture conditions and to define the characteristics that might be indicative of healthy oocyte.

최적의 다층신경망을 이용한 영상 검색 알고리즘

박수봉 東新大學校 1999 論文集 Vol.11 No.-

In this paper, we propose a image search algorithm using back-error propagation learning based on WMRV. We capture images of 256×256 size with gray scale 256 levels from input images which were having monotonous background in the condition of same lumination and distance. After removing noises with a pre-processing process and edge detection, we detect a characteristic region. From this region we extract characteristic vectors using DCT(Discrete Cosine Transform). In case of human face image, we could see edge lineaments are distributed eye brows and a eyes in large numbers. Using this feature we detect a square area including eye brows, eyes, a nose, and a mouse which were contained most of human face information. And we use this square area as input data for multilayer neural network so that recognize a human face after learning process. In DCT processing with a square area which were extracted, the calculation time was reduced 49% than processing of entire facial image. Through simulation results, it shows many improvement in convergence speed. After simulation for 30 persons with 10 images per person(total 300 images), we proved 100% of recognition rate and shows 94% recognition rate for 50 persons which weren't learned by multilayer neural networks.

체외 배양시 과립막세포와 공배양된 돼지 난포란의 성숙과 수정

박수봉,박항균,입곡명 ( S . B . Park,H . K . Park,Akira Iritani ) 한국축산학회 1990 한국축산학회지 Vol.32 No.1

The present study was undertaken to examine the maturation and fertilization of porcine oocytes cocultured with granulosa cells. The results obtained were as follows. The percentages of oocytes reaching metaphase Ⅱ by GC -free culture and coculture with MGC and LGC were 32, 28 and 24% at 33h, respectively. In the culture from 33h to 42h, rates of oocyte maturation showed no difference among experimental groups. The fertilization rates of oocytes matured by GC-free culture and coculture with MGC and LGC were 92, 97 and 94%, respectively, Male pronucleus formation rates of oocytes matured by GC-free culture and coculture with MGC and LGC were 49, 55 and 78% respectively and markedly increased by coculture with LGC. The present results suggest that coculture with the membrana granulosa cells does not affect the incidence of maturation, and that pronucleus formation was promoted through coculture with LGC.

강화학습을 적용한 트롤 어구의 투망 시점 도출

박수봉 한국수산해양기술학회(구 한국어업기술학회) 2018 한국수산해양기술학회 학술발표대회 Vol.2018 No.04

돼지 난포란의 성숙과 수정에 대한 성숙배지에 첨가된 난포액의 영향

박수봉,박항균,입곡명 ( S . B . Park,H . K . Park,Akira Iritani ) 한국축산학회 1990 한국축산학회지 Vol.32 No.2

The purpose of these experiments were to investigate the effect of follicular fluid supplemented to maturation medium on the maturation and fertilization of porcine follicular oocytes. The results obtained were as follows. In the media with MFF and LFF, the rates of maturation division of porcine oocytes in vitro were 82% and 80%, respectively, and the proportion of oocytes matured to metaphase Ⅱ become nearly constant after 39h of culture. However, the rates of maturation of porcine oocytes in medium with FCS 10% were 60% and 79% at 39 and 42h, respectively. The fertilization rate of oocyte cultured in the medium with FCS 10% is 99%. When the oocytes matured in medium with MFF and LFF were fertilized, fertilization rates were 42% and 54,%, respectively. The male pronucleus formation rates of oocytes cultured in the media with FCS 10% MFF 10% and LFF 10% concentrations were 54, 74 and 84%, respectively and were increased through culture in media supplemented with follicular fluid. The present results clearly demonstrate that follicular fluid promoted the maturation of oocytes and male pronucleus formation.