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세포질적 유전자적 웅성불임을 이용한 벼 일대잡종 육성연구 1 : 세포질적 유전자적 웅성불임계통 이용과 일대잡종 육성
徐學洙,李昌垠,許文會 韓國作物學會 1985 Korean journal of crop science Vol.30 No.4
우리나라 수도 품종을 배경으로 한 세포질적 유전적 웅성불임계통들에 우리나라 품종의 임성회복친을 교잡시킨 HR1619A/남풍벼, HR1619A/가야벼, HR1619A/계통234, 통일A/남풍벼, 통일A/청청벼, 수원296A/계통209, 수원296A/계통237, 수원296A/계통252 및 계통201A/계통234 등 9개 Hybrid를 육성하고 이들의 수량 및 수량 구성요소, 흰빛잎마름병 저항성 및 미질을 조사하여 다음과 같은 결과를 얻었다. 1 공시된 9개 Hybrid들 중 계통201A/계통234, 통일A/남풍벼 및 HR 1619A/남풍벼 등 3개 Hybrid의 수량성이 높았는데 각각 우수친에 비해 36%, 17% 및 10%의 증수(Heterobeltiosis)를, 표준품종인 청청벼에 비해 각각 19%, 17% 및 14%의 증수(Standard Heterosis)를 보였다. 2. 공시된 Hybrid중 HR1619A/가야벼와 수원296A/계통237의 수량은 양친보다 낮았다. 3. 수량구성요소 중 수당입수의 잡종강세가 현저하였고 주당수수, 천립중 및 임실율의 잡종강세는 인정되지 않았다. 4. Hybrid의 흰빛잎마름병 저항성은 양친 중 강한친과 같은 반응을 보였다. 5. Hybrid들의 Amylose 함량은 20.8%∼24.7%였고 딘백질함량은 9.3%∼10.0%, 알칼리 붕괴도는 3.7∼7.0으로서 각각 양친품종과 비슷하였다. Nine hybrid rices crossed between Korean cytoplasmic-genetic male sterile rices having the WA cytoplasm and the Korean restorer lines or varieties, HR1619A/Nampungbyeo, HR1619A/Gayabyeo, HR1619A/Line234, TongilA/Nampungbyeo, TongilA/Cheongcheongbyeo, Suwon 296A/Line 209, Suwon 296A/Line 237, Suwon 296A/Line 252 and Line 201A/Line 234, and their parents were grown at Yeungnam University in 1984. The rough rice yield of the hybrids Line 201A/Line 234, TongilA/Nampungbyeo and HR1619A/Nampungbyeo were 939, 927 and 900 Kg/10a respectively. The heterosis(F1 /Midparent) of the above three hybrids was 40%, 20% and 19%, the heterobeltiosis(F1 /Better parent) was 36%, 17% and 10%, and the standard heterosis (F1 /Standard variety, Cheongcheongbyeo) was 19%, 17% and 14% respectively. The hybrids HR1619A/ Gayabyeo and Suwon 296A/Line 237 showed negative heterosis in grain yield. Significant heterobeltiosis for grain number per panicle was found while less or no heterobeltiosis was observed in panicle number per hill, 1000-grain weight and grain fertility. The bacterial leaf blight disease reaction of the hybrids tested was almost the same as that of one parent at least. The amylose content of the hybrids was medium to low the same as their parents. The protein content and alkali digestion value of the hybrids were almost the same as their parents.
The Hypernodulating nts Mutation Induces Jasmonate Synthetic Pathway in Soybean Leaves
서학수,Jinjie Li,이선영,Jae-Woong Yu,김길현,이석하,In-Jung Lee,백남천 한국분자세포생물학회 2007 Molecules and cells Vol.24 No.2
Symbiotic nitrogen fixation with nitrogen-fixing bacteriain the root nodules is a distinctly beneficial metabolicprocess in legume plants. Legumes control thenodule number and nodulation zone through a systemicnegative regulatory system between shoot androot. Mutation in the soybean NTS gene encodingGmNARK, a CLAVATA1-like serine/threonine receptor-like kinase, causes excessive nodule developmentcalled hypernodulation. To examine the effect of ntsmutation on the gene expression profile in the leaves,suppression subtractive hybridization was performedwith the trifoliate leaves of nts mutant ‘SS2-2’ and thewild-type (WT) parent ‘Sinpaldalkong2’, and 75 ESTclones that were highly expressed in the leaves of theSS2-2 mutant were identified. Interestingly, the expressionof jasmonate (JA)-responsive genes such asvspA, vspB, and Lox2 were upregulated, whereas thatof a salicylate-responsive gene PR1a was suppressed inthe SS2-2 mutant. In addition, the level of JA wasabout two-fold higher in the leaves of the SS2-2 mutantthan in those of the WT under natural growthconditions. Moreover, the JA-responsive gene expressionpersists in the leaves of SS2-2 mutant withoutrhizobia infection in the roots. Taken together, ourresults suggest that the nts mutation increases JA synthesisin mature leaves and consequently leads to constitutiveexpression of JA-responsive genes which isirrelevant to hypernodulation in the root.
한국 재래 적미 수집 및 특성 검정 5 : 수심 및 토심에 따른 발아 특성
徐學洙,河雲龜 韓國作物學會 1993 Korean journal of crop science Vol.38 No.2
한국 재래 적미(앵미)는 다양한 유전적 변이와 작물학적 특성이 있을 것으로 추측되어 유전자측으로서의 유용성을 검정하고자 경상남북도에서 수집한 적미중 생웅적으로 특징있는 장입형 적미 10계통, 단입형 적미 10계통 등 20계통과 대조품종으로 삼강벼, 섬진벼를 공시하여 수확후 저장기한에 따른 휴면정도, 수중발아 및 토중발아 등 발아특성을 검정한 결과를 요약하면 다음과 같다. 1. 한국 재래 장. 단입형 적미는 수확직후 99%이상의 발아율을 보여 휴면성이 거의 없었다. 2. 장입형 적미는 수심 7cm에서 83.5%의 식물체의 묘 출현율을 보였고 단입형 적미는 수심 11cm에서 83.5%의 묘 출현율을 보여 실용상 지장이 없는 입묘율이 확보되었으나 대조품종인 삼강벼는 수심 7cm이상에서 섬진벼는 수심 11cm 이상에서 전혀 출현하지 못하였다. 3. 토심 7cm에서 장입형 적미는 71.7% 단입형 적미는 73.9%의 묘 출현율을 보였으며 대조품종인 삼강벼와 섬진벼는 각각 40.0%, 28.0%를 보였다. 4. 한국 재래 적미는 직파재배의 유용 유전자측으로 이용 가능성이 높은 것으로 평가 되었다. This experiment was carried out to evaluate the germination characteristics of Korean red rice seeds. Ten lines of the long grain and ten lines of the short grain Korean red rices were tested for dormancy, shoot emergence rate at different water and soil depth. Both the long and short grain type seeds of Korean red rice germinated over than 99% immediately after harvest, so that no dormancy was observed in the Korean red rices. Shoot emergence rate of the long grain red rices at 7cm of water depth and that of the short grain red rices at 11cm were 83.3% and 83.5% respectively, while those of the indica check Samgangbyeo and japonica check Seomjinbyeo were 0%. Shoot emergence rates of the long and short grain red rices at 7cm of soil depth were 71.1% and 73.9% respectively, while those of the check varieties Samgangbyeo and Seomjinbyeo were 40.0% and 28.0% respectively. Thus, the Korean red rices were expected to be useful germplasm source for direct seeding.