Chromium Trioxide의 독성에 대한 산수유 추출물의 항산화 효과

서영미,Seo, Young-Mi 대한임상검사과학회 2018 대한임상검사과학회지(KJCLS) Vol.50 No.2

본 연구 목적은 산수유 추출물의 항산화 효과를 배양 C6 glioma 세포를 대상으로 조사하였다. 이 같은 목적을 위하여 C6 glioma 세포를 48시간 동안 배양한 후 6가 크롬의 세포독성 및 산수유 추출물의 방어효과를 조사하였다. 이외에 EDA 및 LDH 활성과 같은 항산화 효과를 분석하였다. 본 연구에서 $CrO_3$는 처리농도에 비례하여 세포생존율을 감소시켰다. 또한 이 과정에서 세포를 48시간 동안 20~40 uM로 각각 포함된 배양액에서 처리한 결과 $XTT_{50}$ 값은 33 uM로 나타났다. 항산화제인 CAT는 $CrO_3$로 유도된 세포독성에 의해 감소된 세포생존율을 유의하게 증가시켰다. 한편 산수유 추출물의 보호효과에 있어서, 산수유 추출물은 $CrO_3$만의 처리군에 비하여 세포생존율을 유의하게 증가시켰다. 동시에 산수유 추출물은 EDA와 LDH 활성과 같은 항산화 효과를 보였다. 이와 같은 결과로부터 $CrO_3$의 독성에 산화적 손상이 관련되어 있는 것으로 나타났다. 또한 산수유 추출물은 이의 항산화 효과에 의하여 $CrO_3$의 세포독성을 효과적으로 방어하였다. 결론적으로, 산수유 추출물과 같은 천연소재는 산화적 손상과 관련된 중금속에 의해 유발된 세포독성을 방어 내지는 치료하는데 유용한 치료적 요소의 하나로 생각된다. This study examined the antioxidative effects of Cornis fructus (CF) extract on cultured C6 glioma cells. For this purpose, cytotoxicity analysis of chromium trioxide ($CrO_3$) was performed and the protective effects of the CF extract on $CrO_3$-induced cytotoxicity was examined after the C6 glioma cells were cultured for 48 hours. The antioxidative effects, such as electron donating activity (EDA) and lactate dehydrogenase (LDH) activity were also analyzed. In this study, $CrO_3$ decreased the cell viability in a dose dependent manner. The $XTT_{50}$ value was determined to be $33{\mu}M$ after the cells were treated for 48 hours at a concentrations of $20{\sim}40{\mu}M$ $CrO_3$. The catalase (CAT) antioxidant increased significantly the cell viability that had been decreased by $CrO_3$-induced cytotoxicity. Regarding the protective effect of the CF extract, the cell viability of the CF extract was increased significantly compared to that of $CrO_3$ only. In addition, the CF extract showed antioxidative effects, such as EDA and an inhibitory effect on the LDH activity. These findings suggest that the cytotoxicity of $CrO_3$ is correlated with oxidative stress, and the CF extract effectively prevented $CrO_3$-induced cytotoxicity through the antioxidative effects. In conclusion, natural products, such as the CF extract may be a useful therapeutic agent for the prevention or treatment of toxicity induced by heavy metals via oxidative stress.

입원환아 부모의 간호요구

서영미,권인수,조명옥,최운주,Seo Young Mi,Kwon In Soo,Cho Myeong Ock,Choi Woon Ju 한국아동간호학회 1999 Child Health Nursing Research Vol.5 No.1

The main purpose of this study is to identify nursing needs of parents who have hospitalized child. Research design is a descriptive survey. The subjects for the study were 79 parents who have hospitalized child at a pediatric ward of one of the general hospital attached to a university in J city. The data was collected by researchers using a structured questionnaire. The questionnaire was Likert type 5 point scale, composed of 5 categories with 61 items. The data was analyzed by SPSS/PC. The results of the study were as follows ; 1) Mean score of nursing needs of subjects were 3.93 at 2nd day of admission and 3.99 at 7th day of admission. 2) Among the categories, the highest nursing need at the End day and 7th day of admission was ‘dilect nursing’, in desending order, ‘facilities and environment’, ‘education and counseling’, ‘nursing assessment’. The lowest nursing need was ‘reference’. 3) Differences between the nursing needs of subjects at 2nd day and 7th day were as follows : (1) By categories, there was a significant difference only in the ‘reference’ categoly(t=2.74, P=.008). (2) By items, there were significant differences in items of ‘to check necessary materials(t=2.31, P=.024)’, ‘to understand family function and family relationship(t=2.12, P=.041)’, ‘to set up study room(t=2.22, P=.030)’, and ‘to mediate parent's meeting group(t=3.89, P=.000)’. The above result indicated that nursing needs of parents with hospitalized child were above average, especially very high in items about disease process, and items directly associated with treatment and nursing care. So, nurses have to focus on information about the patient's state of disease, treatment, test and procedure, and in efficiently giving direct nursing care to implement mure effective care for the hospitalized children and their parents. And some future researche is needed to identify the difference of degree of nursing needs of parents with hospitalized child according to admission duration using a different sample and a longer sampling interval.

한국 대학생들의 영어전공서적 읽기에 대한 질적 사례 연구

서영미 ( Seo Young-mi ) 한국현대영어영문학회 2017 현대영어영문학 Vol.61 No.2

This qualitative case study explores how Korean college students feel about reading major textbooks in English and how they read them. Four college students with different majors at one university in Incheon participated in the study. The researcher met the participants individually. They were asked to bring their own major textbooks in English and read the texts. Then, the researcher interviewed each of them about the ways to understand the texts. The interview data were analyzed into categories based on the research questions and several themes were emerged. The data analysis revealed that the students were stressed, felt burden in reading major textbooks in English, and they mainly read them with extrinsic motivations. In terms of reading patterns, the students tended to analyze the passages that they read and used a few reading strategies including predicting or guessing, using prior knowledge, and using text features. One interesting finding is that they often depended on the supplementary materials provided by the instructor. At the same time, they made their own efforts to understand major textbooks in English by searching related materials and/or utilizing translated version of the text if any. Pedagogical implications were also discussed. (Incheon National University)

제2형 당뇨병 성인 남성 환자의 약물치료 이행과 혈당 조절 수준 간에 치료 스트레스의 매개효과

서영미 ( Seo Yeong-mi ),최원희 ( Choi Won-hee ) 경희대학교 동서간호학연구소 2016 동서간호학연구지 Vol.22 No.2

Purpose: The purpose of this study was to examine the mediating effect of regimen distress in the relationship between medication adherence and glycemic control in men with type 2 diabetes. Methods: A total of 116 patients with type 2 diabetes was recruited for the cross-sectional survey design. Data were analyzed using descriptive statistics, independent t-test, ANOVA, Pearson`s correlation coefficient, and multiple regression using Baron and Kenny steps for mediation. Results: The mean score for medication adherence was 6.32, and the mean glycemic control level (HbA1c) was 7.47%. The mean score was 2.37 for regimen distress. There were significant correlations among medication adherence, regimen distress, and HbA1c. Regimen distress had a partial mediating effect (β=.22, p=.005) in the relationship between medication adherence and HbA1c (Sobel test: Z=2.47, p=.013). Conclusion: Regimen distress was found to be associated with glycemic control in men with type 2 diabetes. Based on the findings of this study, nursing intervention programs focusing on decreasing regimen distress are highly recommended to improve level of glycemic control in patients with type 2 diabetes.

Superoxide Dismutase가 배양인체피부멜라닌세포의 산화적 스트레스에 미치는 영향

서영미 ( Young Mi Seo ),김남송 ( Nam Song Kim ) 한국산업보건학회 2009 한국산업보건학회지 Vol.19 No.3

외국인투자자의 특성이 영업권 손상차손 인식 관련 의사결정에 미치는 영향

서영미(Youngmi Seo) 대한경영학회 2020 大韓經營學會誌 Vol.33 No.12

「혹부리영감」의 이데올로기적 활용양상 연구 -일제강점기와 북한 사례를 중심으로-

서영미 ( Young Mi Seo ) 한국어린이문학교육학회 2015 어린이문학교육연구 Vol.16 No.3

본고는「혹부리영감」의 이데올로기적 활용양상을 일제강점기와 북한 초기를 중심으로 고찰하였다. 「혹부리영감」은 1910년 조선총독부에 의해 제작된 『조선물어집』에 최초로 실린 우리나라 민담이다. 이후 일제의 문화정책 중 식민지 아동을 대상으로 한 교육정책에서 활발히 활용된 교재이며 해방 후 북한에서도 아동의 교양교육 교재로 주목 되었다. 이에 본고는 어떤 교육적 가치와 의미가 「혹부리영감」에 내재되어 있는지를 고찰하였다. 그 결과 ‘거짓말’을 매개로 한 교훈성이 도덕성에 중점을 둔 이데올로기 교육에 적합한 교재로 선발 된 요소임을 알 수 있었다. 후반부에 나타나는 다양한 변이는 민담의 해학성과 골계미를 강화 시키며 더불어 선악대비에 따른 교훈성을 부각시킨다. 이러한 교훈성은 ‘착한 어린이’의 특성으로 부각되며 정권에 순응하는 순종적 인간을 양성하는 데 좋은 교재 역할을 하였다. 이에 본고에서는 이러한 특성이「혹부리영감」을 이데올로기적 활용이 가능한 텍스트로 자리하게 함을 밝혔다. 향후 과제로 남한의 경우를 분석하여 비교한다면「혹부리영감」에 내재된 교육적 특성이 보다 선명하게 드러날 것이다. 이는 옛이야기의 가치와 활용이란 측면에서 후세대 교육에 필요한 결과로 이어질 것이다. The purpose of this paper is to find the factors which make it possible to apply the ideological characteristics of <Hokpuri Haraboji> Folk-tale. <Hokpuri Haraboji> is a traditional folk-tale which was first published in <The Folk Tales of Chosun> produced by the Japanese Government-General of Korea in 1910. After then, it was one of the most actively used educational materials to teach Japanese cultural policy for Korean children. In addition, after liberation from Japanese Invasion, it was listed in the catalogue of books which was published as cultural subjects for the children in North Korea. Thus, this paper is aimed to find the ideological application of the educational value and meaning inherent in the <Hokpuri Haraboji> from the perspectives of Folk tale and Ideology. As for results, the author found many variants in the second half of the <Hokpuri Haraboji> which typically belongs to the structure of the imitation. Variants were composed of the keyword, `` lie or fabrication``. This is because `` lie or fabrication`` could function effectively to teach ``the rewarding the good and punishing the wicked`` from the dichotomous ideological viewpoint. This structure shows the value and meaning of teaching materials in facillitating the ‘nice children project’ propaganda during the Japanese invasion, as well as in North Korea. Yet-iyagi keeps many kinds of ``lie-related stories``. But, <Hokpuri Haraboji> has several advantages compared to Yet-iyagi. Being an ``Imitating Story``, it puts great emphasis on the ‘Good and Bad Contrasts Issues’ and Education Issues``. This shows that there exist significant differences between the ``lie in the Hokpuri Haraboji and the other many ``lie-related stories``. Although the addition of many variants to the second half of the Hokpuri Haraboji to emphasize the ‘Good and Bad Contrast’, only increased the humourism and witticism, it did not change any general structure of the story. This is the main reason why <Hokpuri Haraboji> was chosen as appropriate teaching material for the Education of Ideology based on morality. Conclusively, this paper made it clear that <Hokpuri Haraboji> could be used as a textbook for ideological application because of its inherent characteristics. In addition, the result of this paper keeps its important value in the sense that it proved that Yet-iyagi can be used for the purpose of propaganda. In the future, if we compare the stories of South Korea with those of North Korea, the inherent educational characteristics will reveal themselves very clearly. And it will lead to effective education for future generations from the perspective of value and application of Yet-iyagi.

제임스 윈틀 ≪트럼펫과 피아노를 위한 The Key≫(The Key for Trumpet and Piano, 1998)분석적 연구

서영미 ( Young Mi Seo ) 한국피아노학회 2011 피아노음악연구 Vol.5 No.-

James Wintle(b.1942) is one of America`s most successful living composers. Wintle and his compositions have attracted the attention of many prominent performers and scholars over the last three decades. The Key for trumpet and piano was composed in 1988 for Chris Gekker, an outstanding trumpet player. It consists of four movements: a fast movement in free form, a slow lyrical movement in song form (ABA`), a dance-like movement influenced by ragtime, and a fourth movement with a slow introduction in rondo form (ABA`CA``). The purpose of the study is to introduce the composer, James Wintle, and to present an analysis of The Key for trumpet and piano, a work which receives frequent performance. Through research and analytical approaches, the study focuses on a theoretical analysis of The Key for trumpet and piano. In addition to using available materials and resources, the author was in direct contact with James Wintle for the study. Chapter 1 presents the purpose of the study, the state of research, and James Wintle`s biography. Chapter 2 examines Wintle`s compositional style, including influences and musical language, and a theoretical analysis of all four movements of The Key, as well as a discussion of extra-musical influences from the painting entitled The Key by Jackson Pollock. A summation and conclusion follow in chapter 3.

제 2형 당뇨병 환자의 피로와 영향요인

서영미(Seo, Yeong-Mi),최원희(Choi, Won-Hee),함종렬(Hahm, Jong-Ryeal) 한국산학기술학회 2014 한국산학기술학회논문지 Vol.15 No.9

본 연구는 제 2형 당뇨병 환자의 피로정도와 피로에 영향을 미치는 요인을 확인하기 위하여 시도된 서술적 조사연구 이다. 연구는 117명의 제 2형 당뇨환자를 대상으로 하였으며 자료는 2013년 5월1일에서 6월 30일까지 J시에 위치한 일대학병 원에서 면대면 설문지법으로 수집하였다. 수집된 자료는 SPSS WIN 15.0 program을 이용하여 기술통계, t-test, one way ANOVA, Pearson Correlation, 다중회귀분석 등으로 분석하였다. 연구 결과, 대상자의 피로는 2.80점이었다. 피로에 영향을 미치는 요인들은 우울, 나이, 질병이환기간으로 확인되었고 이들 변수는 피로에 대해 40%의 설명력을 보였다. 따라서 건강전 문가들은 제 2형 당뇨환자의 건강관리를 위하여 피로감소 간호중재개발이 필요하며 이러한 간호중재의 보다 나은 효과를 위하여 나이, 질병이환기간을 고려하고 대상자의 우울 감소프로그램을 제공할 필요가 있다. This descriptive study was performed to identify the factors that influence fatigue in patients with type 2 diabetes mellitus. A total of 117 subjects were recruited from a university hospital in J city. For data collection, a questionnaire survey was performed between May 1 and June 28 2013. Data were analyzed by descriptive statistics, t-test, one way ANOVA, Pearson Correlation, and multiple regression analysis using the SPSS WIN 15.0 package. The mean fatigue scores of the subjects was 2.80. Depression, age, and time since the diagnosis were the variables influencing fatigue. The total explanatory power of these factors on fatigue was 40%. In conclusion, it is essential to develop a fatigue control nursing program considering depression, age and time since the diagnosis for patients with type 2 Diabetes mellitus.

치매유발물질인 염화알루미늄으로 손상된 C₆ 신경교종 세포주에서의 항산화 효과

서영미 ( Young-mi Seo ) 대한임상검사과학회 2020 대한임상검사과학회지(KJCLS) Vol.52 No.4

본 연구는 치매유발물질의 하나인 염화알루미늄(AlCl₃)의 신경독성을 C₆ glioma세포를 배양하여 조사하였으며, AlCl₃의 독성에 대한 쑥부쟁이[Aster yomena (Kim.), AY] 추출물의 보호효과를 조사하였다. 또한, 세포생존율을 비롯하여 항산화능 분석인 XO 저해능과 SAR-소거능을 조사하였다. 그 결과 AlCl₃는 배양 세포에 처리한 농도에 비례적으로 세포생존율을 유의하게 감소시켰다. 이 과정에서 XTT₅₀값이 130.0 μM로 나타나 Borenfreund와 Puerner의 독성판정기준에 의하여 중간독성(mid-cytotoxic)으로 나타났다. 한편, 항산화제인 QU는 AlCl₃의 독성으로 손상된 세포생존율을 유의하게 증가시켰다. 또한, AlCl₃의 독성에 대한 AY 추출물의 방어효과에 있어서, AY 추출물은 AlCl₃만의 처리에 비하여 세포생존율을 유의하게 증가시켰으며, 이와 동시에 XO 저해능과 SAR-소거능과 같은 항산화 효과를 나타냈다. 이상의 결과로부터 AlCl₃의 독성에 산화적 손상이 관여하고 있으며, AY 추출물은 항산화 효과에 의하여 AlCl₃의 독성을 효과적으로 방어하였다. 따라서, AY 추출물과 같은 천연물질은 질환유발제인 AlCl₃와 같이 산화적 손상과 관련이 있는 중금속화합물의 독성 완화나 질환의 치료적 개선을 위한 대체물질개발에 있어 유용한 소재인 것으로 생각된다. This study examined the neuronal cytotoxicity of aluminum chloride (AlCl₃), a dementia inducer, and the protective effects of Aster yomena (Kitam.)(AY) extract on AlCl₃-induced cytotoxicity in cultured C₆ glioma cells. The antioxidative effects, such as the inhibitory ability of xanthine oxidase (XO) and superoxide anion-radical (SAR) scavenging ability, on cell viability were examined. AlCl₃ decreased the cell viability significantly in a dose-dependent manner, and the XTT₅₀ value was 130.0 μM in these cultures. The cytotoxicity of AlCl₃ was determined to be mid-toxic according to the Borenfreund and Puerner’ toxic criteria. Quercetin (QU), an antioxidant, increased the cell viability reduced by AlCl₃-induced cytotoxicity. The protective effect of the AY extract on AlCl₃-induced cytotoxicity was analyzed. The AY extract increased the cell viability remarkably compared to the AlCl₃-treated group and showed the inhibitory ability of XO and SAR-scavenging ability. The cytotoxicity of AlCl₃ was correlated with oxidative stress, and the AY extract effectively prevented AlCl₃-induced cytotoxicity through its antioxidative effects. In conclusion, natural resources, such as the AY extract, may be a putative agent for improving the cytotoxicity of heavy metallic compounds correlated with oxidative stress, such as AlCl₃, a morbid agent. Copyright Ⓒ 2020 The Korean Society for Clinical Laboratory Science. All rights reserved.