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서승만,전성준(토론자) 한국공공관리학회 2018 한국공공관리학회 학술대회 Vol.2018 No.12
고령화 사회에 접어들어 다가온 미래가 된 사회구성원의 고령화는 교통정책 분야에서도 변화를 요구한다. 고령운전자의 증가와 함께 사고 발생률도 높아지는 실정이다. 고령운전자의 교통사고 심각성을 인식하고 관련제도 개선이 이루어지고 있지만 여전히 충분하지 못하다. 법을 강화하고 운전자 의식을 선진화시키는 제도개선이 필요하지만 무엇보다 사고발생 원인에서 취약계층이라 할 수 있는 고령운전자의 차별화된 관리와 ‘보수교육’으로 불리는 ‘적극적 재교육’으로써의 교통안전교육을 통해 스스로 능동적인 대처 능력을 기르고 객관적 시각에서의 각자의 사고 위험 정도나 운전능력을 재고해볼 수 있도록 하는 것이 필요하다.
서승만,김이슬,김미주,양승민,김해영 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.7
Centipedes contain pharmacologically active compounds used as important medicinal material. However, the poisons produced by centipedes can cause human diseases; therefore, its use as a food ingredient is prohibited. This is the first report to develop a real-time PCR method for detection of centipedes. The primer and probe targeting the mitochondrial cytochrome c oxidase subunit 1 (COI) gene were newly designed. The specificity was verified using ten species and was confirmed to amplify only the centipede species. The real-time PCR method exhibited good linearity with a high-determination coefficient (R2 = 0.999) and a detection limit was 0.001 ng. The performance of our method was also verified using five real-time PCR platforms under Universal and Fast PCR conditions. Finally, its applicability to processed food was evaluated using binary insect mixtures, and at least 0.1% of centipedes was detected. Therefore, our method can specifically and sensitively detect centipedes in food, contributing to food safety.
서승만,김현재,신민기,홍승진,차재은,김해영 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.3
Genetically modified organisms (GMOs) have been continuously developed for their convenience and productivity. In the past three years, three new GM canola events (MON94100, LBFLFK, and NS-B50027-4) have been developed. To efficiently control these GM canola events, the detection methods were needed. Therefore, the multiplex PCR method combined with capillary electrophoresis was developed for three GM canola events. Ten GM canola, eighteen GM soybean, thirty-two GM maize, and ten non-GM crops were used to evaluate the specificity of the method. The detection limit of the multiplex PCR assay was determined to be 0.005 ng in the DNA mixture and 0.1% in the spiked sample. The aim of this study was to establish multiplex PCR coupled with capillary electrophoresis for the newly produced three GM canola events. The developed method is expected to contribute to monitor the commercially available GM canola events.
서승만,박샛별,김미주,김해영 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.5
Fruit allergies have become more common in recent years, and are now a serious health problem. In this study, a multiplex PCR assay was used to detect potential fruit allergens causing food allergy labeling in Korea. For the detection of these allergens, specific primer pairs were designed to amplify the allergen-coding genes Cyclophilin (tomato), Mdtl 1 (apple), Pru p 2.01A (peach) and Pectin methylesterase inhibitor (kiwi), and primer pair targeting the 18S ribosomal RNA gene was additionally used as an endogenous control. Primer specificity was assessed with 23 plant species. A mixture of DNA from the four fruits was serially diluted and used to determine the sensitivity of the multiplex PCR assay, which was approximately 0.08 ng. Eleven commercial fruit products were evaluated to verify the applicability of the multiplex PCR assay. This assay is expected to be a specific and efficient method for detecting fruit allergens in foods.
서승만(Seung-Man Suh),박샛별(Saet-Byul Park),김미주(Mi-Ju Kim),김해영(Hae-Yeong Kim) 한국식품과학회 2018 한국식품과학회지 Vol.50 No.4
본 연구에서는 견과류로부터 식품 분석에 사용될 DNA를 4가지 방법으로 추출하고 그 효율을 비교하였다. 동일한 양의 시료를 사용하여 추출된 DNA의 양은 CTAB법이 가장 우수한 것으로 확인되었지만, 추출 시간이 수배이상 오래 걸리고 유기용매를 사용한다는 한계점이 있다. 다른 방법들과 DNA 추출 양의 차이가 큰 잣, 캐슈너트, 피스타치오 너트, 땅콩의 시료는 CTAB법이 가장 효율적인 방법으로 판단되며, 호두, 헤이즐넛, 아몬드의 시료는 변형 CTAB법과 실리카 막법이 CTAB법을 대체할 수 있을 것으로 판단된다. 추출된 DNA를 식물 내재유전자 및 각 견과류에 특이적인 유전자를 사용하여 PCR을 진행하였으며, 모든 추출방법에서 DNA가 정상적으로 증폭되는 것을 확인하였다. This study aimed to explore efficient DNA extraction methods using tree nuts. Four different DNA extraction procedures, including silica membrane method, modified silica method, cetyltrimethylammonium bromide (CTAB) method, and modified CTAB method were examined for their relative efficiency in extracting DNA from pistachio, pine nut, almond, hazelnut, cashew nut, walnut, and peanut. The quality and quantity of the extracted DNA were subsequently assessed by spectrometric measurements, gel electrophoresis, and PCR amplifications. CTAB method was the most appropriate one for extracting DNA from pine nut, cashew nut, pistachio, and peanut. However, it could be replaced by the silica membrane method for walnut and modified CTAB method for almond and hazelnut.
김동헌,서승만,김지영,김해영 한국응용생명화학회 2018 Journal of Applied Biological Chemistry (J. Appl. Vol.61 No.4
‘New Breeding Techniques (NBTs)’ have been one of hot issues, since their future will be affected profoundly by national as well as international regulatory landscapes. In this review, we compare characteristics of NBTs with conventional and genetic modification, and analyze genetically modified organism (GMO) regulatory systems in the context of possible regulation of NBTs. NBTs are very heterogeneous in terms of principles, methodologies, and final products. As Living Modified Organisms (LMO) is defined in the Cartagena Protocol on Biosafety (CPB) as an organism containing novel combination of genetic materials obtained by the use of modern biotechnology, CPB as well as other national legislations locate itself somewhere in the middle between product-based and process-based regulations. It is also noted that jurisdictions with regulatory systems more oriented to product-based one tend to be more productive and decide or may decide to exempt site-directed nucleases-1 from GMO regulation. In this context, Korean legislations are reviewed to clarify the commons and differences in GMO definitions. Act on Transboundary Movement of LMO Act, Food Sanitation Act and Agricultural and Fishery Products Quality Control Act are three major acts to regulate GMOs. It is noted that there are differences in the definition of LMO or GM food/products especially between the LMO Act and the Food Sanitation Act. Such differences may cause conflicts between Acts when policydecision regarding the regulation of NBTs is made. Therefore, it is necessary to reorganize legislations before policies regarding the regulation of any techniques from biotechnology are made.