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배준성,한태룡 경희대학교 유전공학연구소 1992 遺傳工學論文集 Vol.4 No.-
ATPase activity of RecA protein is believed to be essential for the strand exchange and DNA repair reactions in E. coli. Chemical modification(Kuramitsu et al., Biochemistry. 23. 2363. 1984) and ATP photolabeling(Banks and Sedgwick. Biochemistry, 25, 5882, 1986) experiments suggest that cysteinyl-129 is one of the most probable residues for the binding and hydrolysis of ATP in RecA protein. Site-directed mutagenesis is employed to change cysteinyl-129 to serine and alanine to minimize any structural alterations. Wild type and mutant RecA proteins were purified and examined for ATPase activity. All the purified RecA proteins showed sigmoidal activity shapes as a function of substrate concentration, indicating that RecA catalyzed ATP hydrolysis is cooperative. Both mutant RecAs displayed almost same V_(max) with wild type RecA but much larger S_(1/2) values(l80 μM for Ser. 120 μM for ,A1a) than wild type(78 μM). These results suggest that cysteinyl-129 may involve in the ATP binding rather than ATP hydrolysis. Further experiments on direct ATP binding and strand exchange reactions are in progress.
Dose Dependent Effects of Intravitreal Triamcinolone Acetonide on Diffuse Diabetic Macular Edema
배준성,박성준,함이룸,이태곤 대한안과학회 2009 Korean Journal of Ophthalmology Vol.23 No.2
Purpose: To evaluate the effect of different doses of intravitreal triamcinolone acetonide on diffuse diabetic macular edema. Methods: In a retrospective study, 44 eyes with diffuse diabetic macular edema were treated with an intravitreal injection of 4 ㎎ (n=12 eyes), 8 ㎎ (n=17) or 25 ㎎ (n=15) of triamcinolone acetonide (TA). Optical coherence tomography, best-corrected logMAR visual acuity and Goldmann tonometry were performed at baseline, 1 week, and 1, 3, 6, 9 and 12 months after treatment. Mean follow-up was 9.8 months (standard deviation=2.3) with a range of 5-12 months. Results: The duration of intravitreal TA effects on macular thickness and visual acuity increased with increasing dosage. An observed increase in intraocular pressure induced by TA was not significantly associated with dosage. Conclusions: In patients with diffuse diabetic macular edema who receive intravitreal TA, effects may last longer after a dosage of 25 ㎎, than after lower doses of 8 ㎎ or 4 ㎎.