부유습지를 이용한 부영양수계 현장 수질개선 효과

박채홍,박명환,최동호,최형주,이준헌,이명훈,황순진 한국하천호수학회(구 한국육수학회) 2013 생태와 환경 Vol.46 No.1

At weekly intervals, we monitored continuous changes in water quality by constructed floating wetland equipped with the four different filter media (sponge, volcanic stone, activated carbon and magnesium hydroxide) in a eutrophic lake from March 2011 to May 2012. We also investigated phyto- and zooplankton communities both in the influent and the effluent water through the floating wetland. Over a 10-month time period, average turbidity (66%), suspended solids (79%) and chlorophyll-a (80%) concentrations were remarkably reduced in the effluent water compared to the influent (P?0.001). The average removal rates of NO2-N and NH3-N were 24% and 20%, respectively (P?0.05). The average removal rates of NO3-N and TN were less than 10% (P¤0.05). On the other hand, the average removal rates of PO4-P and TP were more than 65% (P?0.01). Interestingly, the abundance of phytoplankton in the effluent was decreased about 2.6 times compared to that of the influent, whereas the abundance of zooplankton in the effluent was increased about 3.5 times compared to that of the influent. Overall, particulate matters (SS, Chl-a and TP) and dissolved nutrients (NO2-N, NH3-N and PO4-P) were particularly reduced at high rates. Therefore, application of our constructed floating wetland in a eutrophic lake improved the water quality and demonstrated a potential for algal bloom mitigation.

북한강 수역에서 분리한 남조류 Anabaena circinalis 휴면포자 발아에 대한 환경요인의 영향

박채홍 ( Chae Hong Park ),임병진 ( Byung Jin Lim ),유경아 ( Kyoung A You ),박명환 ( Myung Hwan Park ),황순진 ( Soon Jin Hwang ) 한국하천호수학회 2014 생태와 환경 Vol.47 No.4

Akinete germination may be a starting point of some akinete-producing cyanobacterial blooms in eutrophic freshwater systems. This study examined germinability of akinete of a cyanobacterium Anabaena circinalis isolated from the sediment of the North Han River (Cheongpyung Dam), Korea, under several environmental factors such as temperature (5~25.C), light (0~100 μmol photons m-2 s-1), nutrients (nitrogen and phosphorus) and pH (5~12). The high germination rate appeared at high temperature: ¤55% at 25.C, followed by 15% at 15.C, 10.C and 10% at 5.C. Low light intensity was favorable for akinete germination. Over 45% of germination occurred at low light intensities (5, 15 and 30 μmol photons m-2 s-1), while less than 10% of germination occurred at both 50 and 100 μmol photons m-2 s-1. No germination occurred in the dark condition. Akinete germination rate increased with nutrient (phosphorus and nitrogen) enrichment, and nitrogen addition showed greater effect on the germination compared to phosphorus addition. Akinetes germinated well at neutral or slightly alkaline pH condition (pH 7 and pH 8: ¤55%), but no germination was observed at pH 11~12. The present study demonstrates some favorable ambient conditions of Anabaena circinalis germination, which could provide useful information to study the germination conditions of other Anabaena species or akinete-forming algae and predict its bloom in eutrophic freshwaters.

북한강 수역에서 분리한 남조류 Anabaena circinalis 휴면포자 발아에 대한 환경요인의 영향

박채홍,임병진,유경아,박명환,황순진 한국하천호수학회 2014 생태와 환경 Vol.47 No.4

Akinete germination may be a starting point of some akinete-producing cyanobacterial blooms in eutrophic freshwater systems. This study examined germinability of akinete of a cyanobacterium Anabaena circinalis isolated from the sediment of the North Han River (Cheongpyung Dam), Korea, under several environmental factors such as temperature (5∼25℃), light (0∼100μmol photons m-2 s-1), nutrients (nitrogen and phosphorus) and pH (5~12). The high germination rate appeared at high temperature: >55% at 25℃, followed by 15% at 15℃, 10℃ and 10% at 5℃. Low light intensity was favorable for akinete germination. Over 45% of germination occurred at low light intensities (5, 15 and 30μmol photons m-2 s-1), while less than 10% of germination occurred at both 50 and 100μmol photons m-2 s-1. No germination occurred in the dark condition. Akinete germination rate increased with nutrient (phosphorus and nitrogen) enrichment, and nitrogen addition showed greater effect on the germination compared to phosphorus addition. Akinetes germinated well at neutral or slightly alkaline pH condition (pH 7 and pH 8: >55%), but no germination was observed at pH 11~12. The present study demonstrates some favorable ambient conditions of Anabaena circinalis germination, which could provide useful information to study the germination conditions of other Anabaena species or akinete-forming algae and predict its bloom in eutrophic freshwaters.

담수 규조류의 세포 부피로부터 유기탄소 함량 추정 방법론

박채홍 ( Chae Hong Park ),권대률 ( Dae Ryul Kwon ),박미례 ( Mi Rye Park ),이상득 ( Sang Deuk Lee ) 한국물환경학회 2024 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2024 No.-

팔당호 현장수를 이용한 남조류 Anabaena circinalis의 발 아 및 성장 잠재력 시험

박명환,임병진,서완범,박채홍,김건희,황순진 한국하천호수학회 2015 생태와 환경 Vol.48 No.4

September 2014 and March 2015 at the three sites (PD-1, PD-2 and PD-3) of Lake Paldang. Nitrogen and phosphorus concentrations were higher at PD-2 than at PD-1 and PD-3. TSI (TN) values at the three field sites were in the range of 65~85, which were the level of eutrophic or hypereutrophic state. TSI (TP) also showed high values (49~68), which were the level of mesotrophic or eutrophic state. Akinete germination potential was higher at PD-2 with increased nutrient (nitrogen and phosphorus), and algal growth potential also increased with nutrient enrichment.

급성음낭증에서 외과적 접근의 필요성

김형지,박채홍,이해원,이길호,박준은,송화식 大韓泌尿器科學會 2001 Korean Journal of Urology Vol.42 No.1

담수환경에서 eDNA와 eRNA를 이용한 Microcystin 합성 남조류 탐색 및 세포 내 Microcystin 생합성 활성 변화

김건희,박채홍,조현진,권대률,황순진 한국하천호수학회 2023 생태와 환경 Vol.56 No.1

Targeting Microcystin (MC), which is most abundantly detected in the North-Han River water area, we analyzed the relationship between the MC biosynthesis gene (mcyA gene), cyanobacteria cell density, and MC concentration, derived an RNA-MC conversion formula, and derived the cyanobacteria. The concentration of MC present in cells was predicted. In the North-Han River waters, the mcyA gene was found mainly at downstream sites of the North-Han River after Muk-Hyeon Stream junction, and higher copy numbers were found on average than other sites. In the Uiam Lake waters upstream of the North-Han River, the mcyA gene copy number increased at the Kong-Ji Stream point, and after September, the mcyA gene copy number decreased throughout the North-Han River waters. The expression of the mcyA gene was concentrated in the short period of summer due to the spatio-temporal difference between upstream and downstream water bodies. The mcyA gene expression level was not only highly correlated with MC concentration, but also correlated with the cell density of Microcystis aeruginosa and Dolichospermum circinale, which are known to biosynthesize MC. Six conversion formulas derived based on the RNA-MC relationship showed statistical significance (p<0.05) and exhibited high correlation coefficients (r) of 0.9 or higher. The expression level of MC biosynthesis gene present in eRNA determines the synthesis of cyanotoxin substances in water, quickly quantifies gene activity, and can be fully utilized for early warning of MC development.

베이지안 모델을 이용한 하구수생태계 부착돌말류의 생태 네트워크

김건희,박채홍,김승희,원두희,이경락,전지영 한국수생태학회(구 한국하천호수학회) 2022 생태와 환경 Vol.55 No.1

공검지 고퇴적물(paleo-sediments)에서 남조류 휴면포자 출현 및 형태 분석에 관한 연구

권대률,박채홍,김건희,조현진,이진영,이호일,이상득 한국조류학회I 2023 수생생물 Vol.3 No.1

The purpose of this study was to investigate the distribution of akinete of Nostocales cyanobacteria in sediments in Gonggeom-ji expected to be lakes and wetlands, and to identify past ancient environments through chronological analysis results and akinete ecological features. The investigation target sites were conducted at 3 sites in the Gonggeom-ji area located in Sangju, and drilling was used to collect sedimentary soil. In order to analyze the formation period of the sedimentary layer, a chronological analysis was conducted, and the appearance, separation, and morphological characteristics of cyanobacteria akinete were conducted at intervals of 10cm. Two types of akinete were observed through a microscope, type 1 showed similar results to the length and width of akinete of the Dolichospermum genus mentioned in previous studies, and type 2 showed similar shapes to the length and width of akinete of the Aphanizomenon genus mentioned in previous studies. Therefore, the two types of akinetes from this study could be inferred as akinetes of the Dolichospermum and Aphanizomenon genus. Akinetes of type 1 were observed at St. 1 and 2, while akinetes of type 2 were observed at St. 2 and 3. The era of akinetes appeared in various sections from the 1,800 s to the 1,500 s BC. Cyanobacteria akinetes can be used as fossil indicators, and through these studies, valuable records of past environmental changes in the survey area can be obtained, and paleontological data can be used to interpret environmental changes in the lake. 본 연구는 호소 및 습지로 예상되는 공검지 지역 내 고퇴적물에서 염주말목(Nostocales) 남조류의 휴면포자(akinete)의 분포 및 남조류 발생 기록을 설명하고 연대분석 결과와 휴면포자 생태적 특징을 통하여 과거 고환경에 대해 파악하고자 하였다. 조사 대상 지점은 상주에 위치한 공검지 일대 3개 지점에서 진행하였으며 퇴적토를 채취하기 위하여 시추를 시용하였다. 퇴적층의 형성시기를 분석하기 위하여 연대분석을 진행하였으며, 채집된 샘플을 대상으로 10cm 간격으로 남조류 휴면포자의 출현 유무와 분리 및 형태적 특징 분석을 진행하였다. 현미경을 통해 관찰된 남조류 휴면포자는 두가지 형태가 관찰되었으며, type 1은 Dolichospermum (=Anabaena) 속의 휴면포자의 폭 및 너비와 비슷한 결과를 보였으며, type 2는 Aphanizomenon 속의 휴면포자의 형태와 유사한 특징을 나타냈다. 따라서, 본 연구에서 나온 2개의 휴면포자는 Dolichospermum 속과 Aphanizomenon 속의 휴면포자로 유추할 수 있었으며, type 1의 휴면포자는 St. 1, 2 지점에서 관찰이 된 반면, St. 2, 3에서는 type 2의 휴면포자가 관찰되어 지점 특이적인 분포양상을 보였다. 휴면포자가 출현한 연대는 기원전 1,800년대에서 1,500년대까지 다양한 구간에서 출현하였다. 남조류 휴면포자는 화석지표로 활용할 수 있으며 이러한 연구를 통하여 조사 지역의 과거 환경변화에 대한 귀중한 기록을 획득할 수 있으며 고생물 데이터를 통해 호수의 고환경변화에 대한 해석에 유용하게 이용될 수 있을 것이다

온도가 남조류 Pseudanabaena redekei의 성장과 냄새물질 (2-MIB) 생산에 미치는 영향

김재현,김건희,박채홍,김현진,황순진 한국하천호수학회 2023 생태와 환경 Vol.56 No.2

Cyanobacteria Pseudanabaena strains are known to produce 2-MIB (odorous material) in freshwater systems, thereby causing problems in water use. However, their physiological responses to environmental factors in relation with 2-MIB production is not well explored. This study was conducted to evaluate the effect of temperature on the growth and 2-MIB production of Pseudanabaena redekei. The experimental cyanobacteria strains were separated from the Uiam Reservoir (North Han River) and cultured in the BG-11 medium. Temperature was set to 10, 15, 20, 25, and 30℃ for the experiment, in the reflection of the seasonal water temperature variation in situ. For each temperature treatment, cyanobacterial biomass (Chl-a) and 2-MIB concentration (intra-cellular and extra-cellular fractions) were measured every 2 days for 18 days. Both maximal growth and total 2-MIB production of P. redekei appeared at 30℃. While intra-cellular 2-MIB contents were similar (26~29 ng L-1) regardless of treated temperatures, extra-cellular 2-MIB concentration was higher only in high temperature conditions (25 ~ 30℃), indicating that the extents of 2-MIB biosynthesis and release by P. redekei vary with temperature. The 2-MIB productivity of P. redekei was much higher in low-temperature conditions (10 ~ 15℃) than high temperature conditions (25 ~ 30℃). This study demonstrated that temperature was a critical factor contributing to 2-MIB biosynthesis and its release in cell growth (r=0.605, p<0.01). These results are important to understand the dynamics of 2-MIB in the field and thereby provide basic information for managing odorous material in drinking water resources.