TBTCl 노출에 의한 대복, Gomphina veneriformis (Bivalvia: Veneridae) 외투막의 미세구조적 변화

박정준,김석렬,박명애,이정식,Park, Jung-Jun,Kim, Seok-Ryel,Park, Myoung-Ae,Lee, Jung-Sick 한국패류학회 2011 The Korean Journal of Malacology Vol.27 No.3

Equilateral venus, Gomphina veneriformis exposed to tribultyltin chloride (TBTCl) for 36 weeks was showed ultrastructural changes of the mantle. The fine mantle had 4-folds and its epidermal layer consisted of simple columnar epithelial cells and ciliated cells and secretory cells. Inner and outer epidermal layer covered connective tissue. The mantle exposed to TBTCl at 12 weeks was decreased cilia in the inner epidermal layer, and observed extension of the hemolymph sinus and destruction of the septum. At 20 weeks, it revealed vacuole formation and pycnosis in the cytoplasm, and scattered muscular fiber. After 28 weeks of exposure, the mantle revealed partially degenerative changes in the epidermal layer. In the ciliated cells, basal body was isolated from the cilia and rootlet complex and basal foot were scattered. The sarcolemma had debris fiber. At 36 weeks, it observed degenerative cells that it revealed disappearance of the cilia, atrophic nucleus, poorly membrane and destruction of the cresternae in the mitochondria, and increasing heterophagosome. The outer epithelial cell had necrotic nuclus, numeous lysosome and disappearance of the microvilli. Therefore, results of this study suggested that chronical TBTCl exposure in the Gomphina veneriformis induced the disorders of shell growth and physiological function with histopathological changes of the mantle. TBTCl에 36주 동안 노출된 대복, Gomphina veneriformis 의 미세구조적인 변화를 전자현미경으로 관찰하였다. 대복의 외투막은 가장자리에 4개의 주름을 가지며, 내부와 외부상피층이 단층의 원주형 상피세포, 섬모세포, 분비세포로 구성되어 있었다. 이들 상피층은 혈림프동이 존재하는 결합조직층을 둘러싸고 있었다. 대복의 외투막은 TBTCl 노출 12주째 내부상피층에 존재하는 섬모의 수가 감소하였으며, 혈림프동의 확장과 함께 격막의 파괴가 관찰되었다. 노출 20주째 세포질내의 공포형성, 핵응축과 같은 변화가 관찰되었고, 결합조직층에서는 근섬유다발의 분절이 부분적으로 관찰되었다. 노출 28주째 상피층의 부분적인 붕괴, 섬모의 변화 및 섬모세포에 존재하는 미세구조가 괴사되었다. 결합조직층에 존재하는 다수의 근형질막은 팽창되었으며, 막의 내부에는 근섬유들이 대부분 파괴되어 막 주변에 근섬유 잔여물들이 관찰되었다. 노출 36주째에는 섬모들은 모두 탈락되고, 핵 응축되고, 이형소체의 증가가 나타나는 퇴행성 상피세포가 나타났다. 외부상피층의 상피세포들이 괴상되어 핵이 파괴되고, 세포질 내에는 다수의 용해소체들이 가득 차 있었으며, 미세융모들은 대부분 소실되었다. 따라서 만성적인 TBTCl 노출은 대복의 외투막은 각장성장 및 생리적인 기능의 장애를 유발하는 것으로 생각된다.

마두영약침(馬兜鈴藥鍼)의 천식억제(喘息抑制) 및 면역조절효과(免疫調節效果)에 대(對)한 실험적(實驗的) 연구(硏究)

박정준,김영일,이현,Park, Jung-Jun,Kim, Young-Il,Lee, Hyun 경락경혈학회 2005 대한침구의학회지 Vol.21 No.1

Objectives : The aim of this study was to investigate the Asthma-suppressive and Immune-regulatory effect of AF-HA(Aristolochiae Fructus Herbal-acupuncture) at Joksamni(St36) in OVA(ovalbumin) induced asthma mouse model. Methods : C57BL/6 mice were sensitized and challenged with OVA(ovalbumin) for 12 weeks(once a week). The mice in the OVA-AF-HA group were treated with AF-HA at St36 for the later 8weeks(3times/week). The mice in the OVA-Needle-prick group were treated with single prick with an injection needle at St36 for the later 8 weeks(3times/week). Results : 1. The lung weight and the total cells in lung of the mice treated with AF-HA at St36 decreased significantly compared with those of the OVA-control group. 2. Total leukocytes and eosinophils in BALF of the mice group treated with AF-HA at St36 decreased remarkably compared with those of the OVA-control group. 3. The collagen accumulation in lung of OVA-AF-HA group decreased significantly compared with that of the OVA-control group, 4. The concentrations of IL-4, IL-5, IgE in BALF, and IL-4, IL-5, IL-13 in serum of the mice group treated with AF-HA at St36 decreased significantly compared with those of the OVA-control group. 5. The numbers of $Gr-1^+/CD11b^+\;and\;CD11b^+$ cells in lung of the mice group treated with AF-HA at St36 decreased significantly compared with those of the OVA-control group. 6. The numbers of $CCR3^+,\;CD4^+,\;CD8^+\;and \;CD3e^+/CD69^+$ cells in lung of the mice group treated with AF-HA at 5136 decreased significantly compared with those of the OVA-control group. 7. The mRNA expressions of $TNF-{\alpha}$, IL-4, IL-5, IL13 in lung of the mice group treated with AF-HA at St36 decreased significantly compared with those of the OVA-control group. Conclusion : These results suggest that Aristolochiae Fructus Herbal-acupuncture at Joksamni(St36) may be an effictive therapeutic method to treat asthma.

자연산 조피볼락, Sebastes schlegeli의 소화관에 기생하는 Hysterothylacium sp.와 Anisakis simplex (Nematoda: Anisakidae)의 형태학적 비교 및 숙주의 조직병리학적 반응

박정준,박명애,최혜승,김석렬,Park, Jung-Jun,Park, Myoung-Ae,Choi, Hye-Sung,Kim, Seok-Ryel 한국현미경학회 2011 Applied microscopy Vol.41 No.3

조피볼락, Sebastes schlegeli 소화관 주변에는 다수의 아니사키 스가 기생하고 있었으며, 몇몇 개체들은 간의 표면에 존재하고 있었다. 유전학적 결과 조피볼락에 기생한 아니사키스는 3종으로 분석되었으며, Hysterothylacium sp.가 가장 많이 분포하고 있었다. Hysterothylacium sp.는 Anisakis simplex보다 짧고 가늘었다. 두 종 모두 전방부분에는 lip, 입, 신경환, 분비공, 분비관이 관찰되었다. 두 종 모두 중간부분에는 식도, 위, 장이 존재하고 있었 지만, intestinal ceacum과 ventricular appendage는 Hysterothylacium sp.에서만 관찰되었다. Hysterothylacium sp.의 후방부분 끝에는 conical nodulose apex이 관찰되었지만, A. simplex의 후방부분 끝에는 spine이 관찰되었다. SEM으로 관찰한 결과 Hysterothylacium sp.의 입 주위에는 3개의 lip이 존재하고 있었는데, dorsal lip은 넓이 65 ${\mu}m$ 내외였고, 한쌍의 lateroventral lip은 넓이 60 ${\mu}m$ 내 외였다. Lip의 표면에는 넓이 8 ${\mu}m$ 내외의 유두돌기 한쌍이 존재하고 있었다. Hysterothylacium sp.와 A. simplex의 충체 넓이는 각각 480 ${\mu}m$와 900 ${\mu}m$ 내외로 측정되었다. Hysterothylacium sp. 의 lateral alae는 높이 7 ${\mu}m$ 내외였으며, cornical nodulose apex에 존재하는 돌기는 지름 약 3.3 ${\mu}m$였다. A. simplex의 후방부분 끝 에는 높이 약 20 ${\mu}m$의 spine이 관찰되었다. 조피볼락 장의 내강 에 존재하는 아니사키스들은 대부분 Hysterothylacium sp.였고, 점막주름 근처 혹은 점막하층에 기생하고 있었다. 아니사키스의 기생으로 인하여 숙주의 점막상피에는 다수의 점액세포가 증가하였다. Numerous anisakids were parasitic near the digestive tract of the black rockfish, Sebastes schlegeli and some anisakids observed on the liver of the host. Anisakids in the host were identified three species (Hysterothylacium sp., Anisakis simplex, A. pegreffii) and Hysterothylacium sp. was the high occurrence of anisakid worms in the host. Hysterothylacium sp. was shorter and thinner than A. simplex. Both of anisakids observed lip, mouth, nerve ring, excretory pore and excretory duct in the anterior portion. In the mid portion, anisakids had esophagus, ventriculus and intestine and especially, Hysterothylacium sp. had intestinal ceacum and ventricular appendage. There was conical nodulose apex at the end of the posterior portion in Hysterothylacium sp. and spine in A. simplex. SEM examination revealed that there was three lip near the mouth of Hysterothylacium sp. Dorsal lip was approximately 65 ${\mu}m$ and paried lateroventral lip were approximately 60 ${\mu}m$ in the width. All lips were found double papilla (approximately 8 ${\mu}m$ in the width). The body width of the Hysterothylacium sp. and A. simplex was approximately 480 ${\mu}m$ and 900 ${\mu}m$ respectively. The hight of the lateral alae was about 7 ${\mu}m$ and width of papilla on the cornical nodulose apex was about 3.3 ${\mu}m$ in Hysterothylacium sp. The hight of spine was approximately 20 ${\mu}m$ in A. simplex. There was mainly Hysterothylacium sp. in the intestinal lumen of the host. The nematod worms were parasitic near the mucosal fold and in the submucosal. In the mucosal epidermal layer, it was increased mucous cells by the infection of the parasites.

설사의 병인병기(病因病機)와 침구치료에 대한 문헌적 고찰

박정준 ( Jung Jun Park ),김영일 ( Young Il Kim ),이현 ( Hyun Lee ) 대전대학교 한의학연구소 2004 한의학연구소 논문집 Vol.13 No.1

잔골재 종류가 콘크리트 물성에 미치는 영향

박정준(Park, Jung Jun),류금성(Ryu, Gum Sung),김기훈(Kim, ki hoon),이건철(Lee, Gun Chul),고경택(Koh, Kyung Taek),한천구(Han, Chun Goo) 한국콘크리트학회 2005 한국콘크리트학회 학술대회 논문집 Vol.2005 No.11

광촉매 콘크리트의 질소산화물(NOx) 제거 특성

박정준(Park, Jung Jun),곽종원(Kwark, Jong Won) 한국콘크리트학회 2017 한국콘크리트학회 학술대회 논문집 Vol.29 No.1

마두령약침(馬兜領약鍼)의 천식억제(喘息抑制) 및 면역조절효과(免疫調節效果)에 대(對)한 실험적(實驗的) 연구(硏究)

박정준 ( Jung Jun Park ),김영일 ( Young Il Kim ),이현 ( Hyun Lee ) 대한경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.3

Objectives: The aim of this study was to investigate the Asthma-suppressive and Immuno-regulatory effect of AF-HA(Aristolochiae Fructus Herbal-acupuncture) at Joksamni(St36) in OVA(ovalbumin)-induced asthma mouse model. Methods: C57BL/6 mice were sensitized and challenged with OVA(ovalbumin) for 12 weeks(once a week). The mice in the OVA-AF-HA group were treated with AF-HA at St36 for the later 8 weeks(3times/week). The mice in the OVA-Needle-Prick group were treated with single prick with an injection needle at St36 for the later 8 weeks(3times/week). Results: 1. The lung weight and the total cells in lung of the mice treated with AF-HA at St36 decreased significantly compared with those of the OVA-control group. 2. Total Leukocytes and eosinophils in BALF of the mice group treated with AF-HA at St36 decreased remarkably compared with those of the OVA-control group. 3. The collagen accumulation in lung of OVA-AF-HA group decreased significantly compared with that of the OVA-control group. 4. The concentrations of IL-4, IL-5, IgE in BALF, and IL-4, IL-5, IL-13 in serum of the mice group treated with AF-HA at St36 decreased significantly compared with those of the OVA-control group. 5. The numbers of Gr-1+/CD11b+ and CD11b+ cells in lung of the mice group treated with AF-HA at St36 decreased significantly compared with those of the OVA-control group. 6. The numbers of CCR3+, CD4+, CD8+ and CD3e+/CD69+ cells in lung of the mice group treated with AF-HA at St36 decreased significantly compared with those of the OVA-control group. 7. The mRNA expressions of TNF-α, IL-4, IL-5, IL-13 in lung of the mice group treated with AF-HA at St36 decreased significantly compared with those of the OVA-control group. Conclusion: These results suggest that Aristolochiae Fructus Herbal-acupuncture at Joksamni(St36) may be an effictive therapeutic method to treat asthma.

150 MPa급 강섬유 보강 HPFRCC 배합구성에 관한 실험적 연구

박정준(Park, Jung Jun),문재흠(Moon Jae Heum),박준형(Park, Jun Hyoung),임나리(Im, Na RI),김성욱(Kim, Sung Wook),이장화(Lee, Jang Hwa) 한국콘크리트학회 2015 한국콘크리트학회 학술대회 논문집 Vol.2015 No.5

UHPC 제조 시 실리카 흄 대체재료로서의 고로슬래그 미분말에 대한 검토

박정준(Park Jung Jun),류금성(Ryu Gum Sung),강수태(Kang Su Tae),고경택(Koh Kyoung Taek) 대한토목학회 2007 대한토목학회 학술대회 Vol.2007 No.10

LNG 외조탱크 적용을 위한 SCP 모듈의 휨성능 평가

박정준(Jung-Jun Park),박기준(Gi-Joon Park),김성욱(Sung-Wook Kim),김언(Eon Kim),신동규(Dongkyu Shin) 한국산학기술학회 2019 한국산학기술학회논문지 Vol.20 No.1

극한지에서LNG 저장구조물을 건설할 때현장 타설 방식으로 제작 및 시공되는경우, 열악한작업 환경 및 조건으로 의해 공사금액 및 공사기간이 증가되는 문제점이 발생할 수 있어 모듈형 에너지 저장탱크에 대한 요구가 높아지고 있다. 이에 본 연구에서는LNG 연료 저장탱크의 경량화를 위해 SCP 모듈의사용을 대안으로 제시하고자 하였다. 이에SCP 모듈을 두께별로 제작하고 그에 따른 휨성능을 평가함으로써 SCP 구조의 LNG 연료 저장탱크에 대한 현장적용 가능성을 평가하고자 하였다. 실험 결과 두께 100mm 실험체는 설계상 극한하중인 413 kN까지는 선형을 유지하면서 증가하다가 기울기가 급격하게 변하면서 파괴되었다. 두께 200mm 실험체 또한 설계상 극한하중인 약 822 kN까지 선형을 유지하다가 파괴양상을 보였다. 두 조건 모두 SCP의 휨 시험에서 INCA guidance의 기준대로 철판이 항복에 도달할 때까지 선형 거동을 나타내었다가 극한하중이후에는 급격한 철판 항복을 동반하는 거동을 하였다. 또한, 목표로 제시한 설계 휨강도를 만족하여 SCP 모듈을 활용하여 LNG 저장탱크 외조구조물에 적용이 가능할 것으로 판단되었다. 추후 압축, 전단 시험 등을 추가로 진행하여 SCP의 구조성능에 대한 평가가 필요할 것으로 판단된다. When constructing LNG storage structures using the cast-in-place method in extreme areas, the construction cost and time may be increased due to the poor working environments and conditions. Therefore, demand for modular energy storage tanks is increasing. In this study, we propose using an SCP module as an alternative for lighter-weight LNG storage tanks. The purpose of this study is to evaluate the feasibility of LNG storage outer tanks by performing bending tests on the thickness of composite steel plate concrete under field conditions. The loads on specimens with thicknesses of 100 mm and 200 mm were linearly increased to the design final loads of 413 kN and 822 kN, respectively. The slope was rapidly changed, and fracture occurred. The two test conditions showed linear behavior until the steel plate yielded, and after an extreme load behavior, sudden yielding of the steel plate yield occurred in the SCP bending test according to the INCA guidelines. The results satisfied the design flexural load and showed the possibility of using the specimens in a modular LNG outer tank. However, it is necessary to evaluate the structural performance of the SCP by performing compression and shear tests in future research.