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족관절 관절경 수술 후 발생한 전경골 동맥의 가성 동맥류
전태환,박용석,김양태,성기선,Chun, Tae-Hwan,Park, Yong-Serk,Kim, Yang-Tae,Sung, Ki-Sun 대한족부족관절학회 2012 대한족부족관절학회지 Vol.16 No.4
Pseudoaneurysm is extremely rare complication after ankle arthroscopy with standard anteromedial and anterolateral portals. We report a case of a pseudoaneurysm of the anterior tibial artery detected at 3 months after ankle arthroscopy in a 16-year-old male. He had sustained painful swelling of his right ankle after the arthroscopic surgery, and referred to our hospital with an MRI checked postoperatively. We failed to make the diagnosis of pseudoaneurysm with the postoperative MRI, thus the patient underwent another arthroscopy which revealed massive hemarthrosis within the joint. The diagnosis was confirmed with an angiography, and the vascular lesion was ligated.
Taxol의 방사면역측정을 위한 I-125 표지화합물 합성
오옥두(Ok-Doo Awh),금준섭(Jun-Sub Kum),이양호(Yang-He Lee),박용석(Yong-Serk Park),편웅범(Woong-Beum Pyun),최창운(Chang-Hoon Choi) 대한의생명과학회 1997 Biomedical Science Letters Vol.3 No.2
Taxo은 diterpenoid구조를 가진 항암제로서, 난소암과 유방암에 탁월한 효과를 보이지만 다른 항암제와 마찬가지로 독성을 가지고 있어 약물의 체내 혈중농도를 모니터링하는 것이 필요하다. 약물의 혈중농도를 모니터링하는 방법은 HPLC법, ELISA법, RIA법 등이 있으나, RIA법이 민감도 측면에서 또한 간편하다는 점에서 장점이 있다. 본 연구에서는 I-125 표지항원을 이용한 방사면역측정법을 확립하기 위해 먼저 taxol유도체를 합성하였다. 먼저 taxol의 C-13 탄소의 곁가지에 위치한 C-2’부분의 hydroxy기를 succinic anhydride와 반응시켜 2'-hemisuccinyltaxol (Ⅰ)을 합성 (반응수율: 80%)하였다. 또한 tyramine을 ¹²?I로 표지하고 gel chromatography를 통해 정제된 [¹²?I]iodotyramine (Ⅱ) (반응수율: 58%)을 얻었다. (Ⅰ)과 (Ⅱ)를 반응시켜 2’-[¹²?I]iodotyramine-hemisuccinyltaxol (Ⅲ) (반응수율: 96%)을 얻어 ¹²?I 표지창원으로 사용하였다. Taxo에 대한 항체를 획득하기 위해서 (Ⅰ)을 BSA에 접합 반응시켜 2'-hemisuccinyltaxol-BSA접한체를 합성하였으며, 이것을 토끼에 면역주사하여 anti-taxol serum을 얻었다. 이 항체에 대한 역가 검정실험에서 1:20의 회석비에서 B/F(%)가 약 40%를 보였다. 이와 같은 결과는 2-[¹²?I]iodotyramine-hemisuccinyltaxol을 표지항원으로 한 taxol의 방사면역측정 방법으로 혈청내 taxol의 농도측정이 가능함을 제시 해 준다. Taxol, an anticancer drug that has diterpenoid conformation, has been used as an effective chemotherapeutical agent in the treatment of breast and ovarian cancers. Because of its toxicity like other anticancer drugs, monitoring the taxol level in serum is important procedure during cancer therapy. The various monitoring methods using HPLC, ELISA, and RIA have been adopted, and RIA technique is known to be superior than other methods in trems of sensitivity and convenience. In this study, in order to develope taxol RIA system using ¹²?I labelled antigen, first of all we synthesized taxol derivatives. 2'-hemisuccinyltaxol was obtained with about 80% yield by esterification of taxol at C-2' hydroxyl group on C-l3 carbon with succinic anhydride. [¹²?I] iodotyramine was prepared with 58% labelling yield by radioiodination of tyramine and purified by gel chromatography. 2'-[¹²?I]iodotyramine-hemisuccinyltaxol, ¹²?I labelled antigen for taxol RIA, was synthesized with 96% yield from conjugation of 2'-hemisuccinyltaxol and [¹²?I] iodotyramine. Anti-taxol serum was produced from the rabbit immunized with 2'-hemisuccinyltaxol-BSA synthesized by 2'-hemisuccinyltaxol and BSA. The antiserum titer was determined by RIA using 2'-[¹²?I]iodotyramine-hemisuccinyltaxol. The titer of 1:20 was obtained with about 40% of B/T. The results suggest that taxol RIA using ¹²?I labelled antigen can be applied to monitor the taxol level in serum.
SiHa 세포주에서 비소화합물 ( As2O3 , As4O6 ) 투여 후 cDNA microarray 를 이용한 유전자 발현의 변화
남궁성은(Sung Eun Namkoong),서미영(Mi Young Seo),박은경(Eun Kyung Park),팽기영(Gi Young Pang),김종국(Chong Kook Kim),박용석(Yong Serk Park),안웅식(Woong Shick Ahn),이준모(Jun Mo Lee),김도강(Do Gang Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.7
Objective : To obtain information on the growth inhibition effect of arsenic compounds and gene expression profiles using cDNA microarray technique in SiHa cell lines. Methods : We cultured 103 SiHa cell in 96 well plate and we investigated growth inhibition effects using MTT assay and also we performed gene expression profile experiment using 384 cDNA chip in SiHa cell after exposure of arsenics (As2O3, As4 O6 - 1 μM) for 48 hrs. Results : Arsenics (As2O3, As4 O6) inhibit the growth of SiHa cells (As2O3: 0.5, 1, 2, 3, 4, 5 μM - 9.2, 56, 89, 93, 96, 96%, As4O6: 0.5, 1, 2, 3, 4, 5 μM- 54, 84, 84, 85, 85, 87%) in 4 days culture. As2O3 and As4O6 induced apoptosis in SiHa cells. After exposure of As2O3, 47 genes were changed more than 2 times (eg, thymidylate synthetase, cyclin B1, CDC 20). In case of As4O6, 78 genes were changed more than 2 times (eg, CDC 20, cyclin B1, primase, proliferating cell nuclear antigen). Conclusion : we observed arsenic compound (As2O3, As4 O6) inhibit the growth of SiHa cell. In gene expression profiling experiment, 78 genes was changed the expression level 2 times more than that of reference RNA after treatment of As4O6 and 47 genes after treatment of As2O3. Through these result, we thought more study need in functional genomics after arsenic treated cervical cancer cells.
자궁경부암 치료에서 p53 종양억제유전자의 플라스미드와 아데노바이러스를 이용한 유전자 치료법의 개발
이준모(Jun Mo Lee),김승조(Seung Jo Kim),남궁성은(Sung Eun NamKoong),조성대(Sung Dae Cho),황성진(Seong Jin Hwang),박현라(Hyun Ra Park),한유진(You Jin Han),김상태(Sang Tae Kim),이헌영(Hun Young Lee),김동재(Dong Jae Kim),박용석(Yong Serk 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.9
Background: The basic treatment of malignant tumors is surgery, radiotherapy, chemotherapy. Even though, the object of these treatments is to kill cancer cells, they have limitations. So, in future studies of treatment of cancer, we should look into increasing human immune response using gene therapy in order to induce damage to tumor cells. Objective: The cell growth inhibitory effect of cervical cancer cells was investigated by direct transfection using liposome(pRcCMVp53/lipofectin). and by indirect transfection using Adenovirus(AdCMVp53). Methods: The cervical cancer cell lines we used in this study were HPV16 positive, having inhibitory gene, wild p53 gene, CaSki, SiHa, HPV18 positive HeLa, HeLaS3 and HPV negative C33A, HT3, LacZ gene was used as the marker gene for the transfection efficacy. Direct transfection was done by using lipofectin (pRcCMVp53/lipofectin) and indirect transfection was done by using virus, AdCMVp53. The effect of tumor cell growth inhibition was measured by cell counting assay. Result: Inhibition of growth of cervical cancer cells in cell counts of direct transfection was CaSki(88.5%), SiHa(59.1%), HeLa(86.0%), HeLaS3(78.0%), C33A(91.3%) and HT3(74.0%). Inhibition of growth of cervical cancer cells in cell counts of indirect transfection was CaSki(97.4%), SiHa(91.6%), HeLa(95.8%), HeLaS3(99.7%), C33A(97.3%) and HT3(87.4%). Conclusion : The inhibition of cell growth of cervical cancer cells by direct and indirect transfection was significantly reduced, and showed little differences depending on the type of cells. These results will have a great meaning in treating cervical cancer patients using gene therapy by direct or indirect transfection
Taxol의 방사면역측정을 위한 I-125 표지화합물 합성
오옥두,최창운,금준섭,박용석,이양호,편웅범 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1997 Journal of biomedical laboratory sciences Vol.3 No.2
Taxol은 diterpenoid구조를 가진 항암제로서, 난소암과 유방암에 탁월한 효과를 보이지만 다른 항암제와 마찬가지로 독성을 가지고 있어 약물의 체내 혈중농도를 모니터링하는 것이 필요하다. 약물의 혈중농도를 모니터링하는 방법은 HPLC법, ELISA법, RIA법 등이 있으나, RIA법이 민감도 측면에서 또한 간편하다는 점에서 장점이 있다. 본 연구에서는 I-125 표지항원을 이용한 방사 면역측정법을 확립하기 위해 먼저 taxol유도체를 합성하였다. 먼저 taxol의 C-13 탄소의 곁가지에 위치한 C-2'부분의 hydroxy기를 succinic anhydride와 반응시켜 2'-hemisuccinyltaxol(Ⅰ)을 합성(반응수율:80%)하였다. 또한 tyramine을 125I로 표지하고 gel chromatography를 통해 정제된 [125I]iodotyramine(Ⅱ)(반응수율:58%)을 얻었다. (Ⅰ)과 (Ⅱ)를 반응시켜 2'-[125I]iodotyramine-hemisuccinyltaxol (Ⅲ) (반응수율:96%)을 얻어 125I 표지항원으로 사용하였다. Taxol에 대한 항체를 획득하기 위해서 (Ⅰ)을 BSA에 접합반응시켜 2'-hemisuccinyltaxol-BSA접합체를 합성하였으며, 이것을 토끼에 면역주사하여 anti-taxol serum을 얻었다. 이 항체에 대한 역가 검정실험에서 1:20의 희석비에서 B/F(%)가 약 40%를 보였다. 이와 같은 결과는 2'-[125I]iodotyramine-hemisuccinyltaxol을 표지항원으로 한 taxol의 방사면역측정 방법으로 혈청내 taxol의 농도측정이 가능함을 제시해 준다. Taxol, an anticancer drug that has diterpenoid conformation, has been used as an effctive chemotherapeutical agent in the treatment of breast and ovarian cancers. Because of its toxicity like other anticancer drugs, monitoring the taxol level in serum is important procedure during cancer therapy. The various monitoring methods using HPLC, ELISA, and RIA have been adopted, and RIA technique is known to be superior than other methods in trems of sensitivity and convenience. In this study, in order to develope taxol RIA system using 125I labelled antigen, first of all we synthesized taxol derivatives. 2'-hemisuccinyltaxol was obtained with about 80% yield by esterification of taxol at C-2' hydroxyl group on C-13 carbon with succinic anhydride. [125I]iodotyramine was prepared with 58% labelling yield by radioiodination of tyramine and purified by gel chromatography. 2'-[125I]iodotyramine-hemisuccinyltaxol, 125I labelled antigen for taxol RIA, was synthesized with 96% yield from conjugation of 2'-hemisuccinyltaxol and [125I] iodotyramine. Anti-taxol serum was produced from the rabbit immunized with 2'-hemisuccinyltaxol-BSA synthesized by 2'-hemisuccinyltaxol and BSA. The antiserum titer was determined by RIA using 2'-[125I]iodotyramine-hemisuccinyltaxol. The titer of 1:20 was obtained with about 40% of B/T. The results suggest that taxol RIA using 125I labelled antigen can be applied to monitor the taxol level in serum.