Lactobacillus rhamnosus BHN-LAB105를 이용한 발효 엉겅퀴의 항산화 및 미백효 과에 대한 유용성 평가

박예은,권기석,김병혁,이중복 한국피부과학연구원 2019 아시안뷰티화장품학술지 Vol.17 No.1

Purpose: This study aimed to increase the effectiveness of the cosmetic functions of fermented thistle (Cirsium japonicum) roots, flowers, and whole plant’s extracts with Lactobacillus rhamnosus (L. rhamnosus) BHN-LAB105 and to evaluate the efficacy of the fermented extracts as cosmetic materials. Methods: Samples included methanol extracts of roots, flowers, and whole thistle plant. Total polyphenol and flavonoid contents of each extract were quantified from gallic acid and rutin. The antioxidative effect of the extract was confirmed using a 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Moreover, the whitening effect of each extract was measured using tyrosinase inhibition activity assay in cell-free level. Results: The total polyphenol contents of fermented root and whole thistle extracts were higher (67%) compared to nonfermented root and whole thistle extracts (32%). The total flavonoid contents were also higher in all fermented extracts of thistle. The DPPH radical scavenging activity of fermented roots, flowers, and whole thistle extracts was higher by 67%, 13%, and 32% compared to nonfermented extracts, respectively. The tyrosinase inhibition activity was higher by 92%, 78%, and 65% for fermented roots, flower, and whole thistle extracts, respectively. Conclusion: The highest antioxidative and whitening effects were investigated in the fermented whole thistle extracts. As a result, fermented whole thistle extracts with L. rhamnosus BHN-LAB105 could be developed as a potential functional cosmetic material. 목적: 본 연구는 Lactobacillus rhamnosus (L. rhamnosus) BHN-LAB105를 이용하여 엉겅퀴의 뿌리, 꽃과 전초를 발효한 발효추 출물의 화장품 소재로서의 효능을 높이고, 화장품 원료로서의 효과를 평가하고자 한다. 방법: 연구에 사용한 엉겅퀴의 뿌리, 꽃, 전초 발효추출물은 메탄올을 이용하여 추출하였다. 총 폴리페놀과 총 플라보노이드 함량은 gallic acid와 rutin에 의해 각각 정량하였 다. 추출물의 항산화능은 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay를 통해 확인하였다. 또한, 추출물의 미백효과는 tyrosinase inhibition activity assay를 통해 분석하였다. 결과: 발효를 통한 엉겅퀴 뿌리 및 전초 추출물의 총 폴리페놀 함량은 발효하지 않는 엉겅퀴 추출물에 비해 각각 67% 와 32% 증가하였다. 또한, 총 플라보노이드는 모든 발효 추출물에서 증가하는 것을 확인했다. 엉겅퀴 발효 추출물의 DPPH 라디칼 소거능은 발효 후 엉겅퀴 뿌리, 꽃, 전초 추출물에서 각각 67%, 13%, 32% 증가하였다. Tyrosinase 저해 활성은 발효 각 부위에서 각각 92%, 78% 및 65% 증가되는 것으로 분석되었다. 결론: 항산화능과 미백효과는 발효 엉겅퀴 전초 추출물에서 가장 높게 나오는 것으로 분석되었다. 이로써 L. rhamnosus BHN-LAB105로 발효된 엉겅퀴 전초 추출물은 기능성 화장품 소재의 후보물질로서 발전 가능할 것으로 판단된다.

3D 프린팅용 Fe/PLA 복합 필라멘트 구조 분석 및 압출성

박예은,이선희 국방기술품질원 2023 국방품질연구논집 Vol.5 No.1

In this study, the characteristics of Fe/PLA and annealed Fe/PLA filaments were analyzed to develop 3D printing products for military defense technology. The most suitable printing conditions were determined by extruding at seven different temperatures determined by the morphology analysis, and 3D printing samples were produced to confirm the magnetic properties of the output. The results of the thermal and physical properties analysis of Fe/PLA and annealed Fe/PLA showed that PLA and Fe had a crystal structure regardless of the annealing treatment, and Fe was 11% contained in Fe/PLA. After 1 h of annealing at 100 ℃, the diameter and thickness increased, and the glossiness improved. The diameter and thickness of the filament after extrusion were similar to the nozzle diameter, and uniform output was desired. During printing, the nozzle temperature was 200 ℃ for Fe/PLA and 210 ℃ for annealed Fe/PLA. 3D printing samples were modeled, converted into .g-code, and output with nozzle temperatures of 200 ℃ and 210 ℃ and bed temperature of 60 ℃ for Fe/PLA and annealed Fe/PLA, respectively, with a size of 25 × 25 × 2 mm3, printing speed of 60 mm/s, zigzag filling pattern, and filling densities of 50% and 100%. Annealed Fe/PLA was 0.2 g heavier than Fe/PLA with a uniform surface. When the magnet was brought close to the output, the magnetic properties analysis showed that the output made of Fe/PLA and annealed Fe/PLA were magnetic objects. The magnetic force was greater for the annealed Fe/PLA output, and neodymium magnets showed magnetic force regardless of the filament type, filling density, surface, and side of the output. Therefore, the possibility of producing FFF 3D printing with magnetic materials such as iron-filled PLA filament was confirmed. Future research will focus on modeling important military parts and devices, such as sensors, magnetic storage devices, weapon parts, and catalytic materials, and evaluating the quality of 3D printing production and quality assessment in the defense field through research on various output density and pattern conditions.

팬데믹 이후, 산 자들의 죽음 이해 : 레비나스를 중심으로

박예은 글로벌지식융합학회 2021 글로벌지식융합학회 학술대회 자료집 Vol.2021 No.2

현대인의 건강증진을 위한 국내 도시공원 액티브디자인 분석

박예은,박진희,황연숙 한국실내디자인학회 2022 한국실내디자인학회논문집 Vol.31 No.5

Due to the development of medical and scientific technology, people are living longer than ever before. With these additional years of life, people are showing a renewed interest in health-promoting physical activities but are seeking to do so in a time efficient manner, as they go about their daily lives. Therefore, this study analyzed environments in which people can naturally and sustainably improve their health. Previous studies were used to examine the importance of modern people’s health activities and the use of urban parks. We used the New York Active Design Guidelines and Seoul Urban Design Guidelines to create an active design checklist for domestic urban parks, and based on the checklist, we analyzed the active design of the six domestic urban parks: Seoullo7017, Son Kee-chung Sports Park, Training Center Park, Munjeong Neighborhood Park, Asia Neighborhood Park, and Ogeum Neighborhood Park. Our analysis revealed a need for significant design improvements. Firstly, various internal facilities and guidance facilities should be provided in order to increase the openness and publicity of the park. Secondly, all pedestrians, including the disabled, should be able to engage in safe and enjoyable activities which promote the continued use of physical exercise. In the future, further research will be needed to promote the ‘Seoul Active Design Guidelines’ to ensure parks match the needs and characteristics of the citizens of Seoul.

현대 업무시설 로비의 공공적 특성에 관한 연구

박예은,전소영,황연숙 한국실내디자인학회 2022 한국실내디자인학회논문집 Vol.31 No.3

Office buildings have contributed to improving the publicity of urban spaces. In particular, the lobby of office building is not just a transfer space, but a public space that accommodates various functions of a complex building. The purpose of this study is to analize the publicity characteristics of lobby in office buildings, and to provide basic data on the planning elements of lobby in office buildings. As a research method, previous studies on the concept of office lobby publicity were analyzed and field investigation methods for six office buildings in Seoul were conducted. The research result are as follows. First, the lobby of office buildings is a public space with open spaces and various convenience facilities, but it shows the limitation of publicity in that the time to use the lobby is limited. Therefore, public spaces such as libraries and exhibition halls extend their opening hours, facilitating access for more people. Second, it is generally easy to enter from various directions, but in some cases, direct access to boulevard and streets is insufficient. When accessibility is relatively low, canopy or pilotis are configured at the entrance to increase cognition. Third, the lighting flowing into the indoor spaces as a whole is good, but it does not provide lively nature. In order to increase the comfort through the nature of the space without visual blocking, it is possible to feel nature indirectly through natural finishes, colors, and shapes. Fourth, it is necessary to improve the movement path more efficiently as it is judged that the direction finding element is insufficient in half of the case sites. To this end, the flow of the movement path is made natural by indirectly inducing the movement path such as the structure of the space, finishing materials, furniture, lighting, etc., or by placing kiosks and signage.

청소년의 완벽주의와 분노표현방식의 관계에서 부․모 양육태도의 조절효과

박예은,진미경 한국청소년상담복지개발원 2014 청소년상담연구 Vol.22 No.2

The purpose of this study was to examine the moderating effects of parental rearing attitudes between perfectionism and anger expression of adolescents. Participants were 520 adolescent 1st, 2nd, and 3rd grade in middle schools. The data collected in the study was analyzed using SAS 8.2 statistic program. As results of phased multiple regression analysis and two-way ANOVA to prove regulation effect of parenting attitude in the relation of perfectionism and anger expression methods, ‘monitoring’, ‘physical abuse’, and ‘neglect’, of fathers’ parenting attitude, and ‘over-expectation’, ‘physical abuse’, and ‘neglect’, of mothers’ showed meaningful regulation effects in the relation of perfectionism and anger-control. Next, for the relation of perfectionism and anger-out, regulation effects of ‘inconsistency’ of fathers’ parenting attitude and ‘over-expectation’ of mothers’ were meaningful. Lastly, for the relation of perfectionism and anger-in, regulation effects of fathers’ and mothers’ parenting attitude were appeared to be meaningful. 본 연구는 청소년의 완벽주의와 분노표현방식의 관계에서 부․모 양육태도의 조절효과 를 규명하고자 하였다. 이를 위해 중학생 545명을 대상으로 아동․청소년 완벽주의 척 도, 부모 양육태도 척도, 한국판 상태-특성 분노표현척도로 구성된 설문을 실시하였다. 본 연구에서 수집된 자료는 SAS 8.2 통계 프로그램을 사용하여 상관분석으로 변인들 간 의 관련성을 검증하고, 단계적 중다회귀분석과 이원변량분석을 통해 조절효과를 검증하 였으며, 그 결과를 요약하면 다음과 같다. 완벽주의와 분노조절의 관계에서 부 양육태도 중 ‘감독’, ‘학대’, ‘방임’이 모 양육태도 중 ‘과잉기대’, ‘학대’, ‘방임’의 조절효과가 유의 하였으며, 완벽주의와 분노표출의 관계에서 부 양육태도 중 ‘비일관성’이, 모 양육태도 중 ‘과잉기대’의 조절효과가 유의하였다. 하지만 완벽주의와 분노억제의 관계에서 부와 모 양육태도의 조절효과는 유의하지 않았다. 이와 같은 연구결과를 통해 완벽주의와 분 노표현방식의 관계에서 부․모 양육태도의 조절효과를 확인할 수 있었다. 보다 자세한 연구결과와 함께 본 연구가 갖는 제한점, 시사점 및 향후 연구방향에 대해 논의하였다.

청송앓이 - 경상북도 시골형 MICE 산업 육성방안 모델개발 -

박예은,박선영,이은서,한솔비 한국관광서비스학회 2016 한국관광서비스학회 학술대회 Vol.- No.27

1. 제안배경 및 필요성 청송지역을 다양한 방향으로 여건 분석 해 본 결과, 청년층과 중ᆞ·장년층의 인구는 점 점 줄어들지만, 노년층은 증가하고 있는 추세이다. 청송을 방문한 관광객의 추이를 살펴본 결과, 2011년에 많은 성장세를 보였지만 그 이후 2014년부터 점차 감소하고 있다. SWOT 분석 결과, 청송이 비교적 낮은 지역 인지도를 갖고 있지만 청송만의 다양한 자연 및 문화적 자원과 숙박시설을 이용해 새로운 기회로 전환할 수 있다고 예상하였다. MICE산업은 문화적, 경제적 파급효과가 높아 국내외적으로 관심이 증가하고 있는 추 세이다. 이러한 시점에서 지역사회에 숙박시설의 개관은 차별화된 MICE산업의 특화도시로 거듭나기 위한 기회이며 청송의 새로운 이미지 창출의 기회이다. 이에 본 팀은 청송만의 이미지 확립의 필요성을 인식하고 청송의 자연 및 문화적 배경 을 바탕으로 '쉼' 과 '놀다' 라는 컨셉을 도출하게 되었다. 2. 홍보방안 R&B 대학생 서포터즈 선발 및 운영 실시간, 쌍방향 커뮤니케이션 채널 개설, 기자, 교 수, 파워블로거, 기업체 교육 담당자 등 전문가 초청 팸투어 진행, 경상북도 교육청에 자율 학기 프로그램 소개 및 중고등학교에 안내문 전달 3. 기대효과 청송의 강점과 기회를 살려 다양한 MICE프로그램의 개발과 홍보를 통해 차별화된 MICE 산업도시로 발돋움하게 되면서 일자리 창출, 지역민 자긍심 고취 그리고 인구 유입 등을 효과를 얻을 수 있다. 지역경제의 발전도시재생을 기반으로 도시재생산이 가능할 것으 로 예상되며 이러한 도시재생산과정은 도식으로 표시하면 다음과 같다.

세대 차이에 따른 스토킹 인식 분석: 베이비붐 세대, X세대, M세대, Z세대를 중심으로

박예은,곽대훈 한국범죄심리학회 2022 한국범죄심리연구 Vol.18 No.3

Existing previous studies that investigated stalking perception focused on investigating specific age groups such as college students rather than various age groups. However, as can be seen from the phrase contemporary asynchronism, people in one society live with different values. Therefore, the purpose of this study is to empirically verify how the perception of stalking differs between generations by classifying the generation who shared a specific historical experience into the baby boomers, Generation X, Generation M, and Generation Z. Using a South Korean adult sample(n=336), we examine stalking perceptions according to generation differences were analyzed. The results show that there were statistically significant differences between generations in the classification of stalking punishment opinions and stalking behavior. Implications for practice and future research are discussed. 스토킹 인식을 조사한 기존 선행연구들은 다양한 연령층보다는 대학생과 같은 특정 연령층을 대상으로 조사하는 것에 중점을 두었다. 그러나 동시대의 비동시성이라는 말에서 알 수 있듯이 한 사회 내에서 사람들은 다른 가치관을 가지고 살고 있다. 따라서 본 연구는 특정한 역사적 경험을 공유한 세대를 베이비붐세대, X세대, M세대, Z세대로 구분하여 스토킹에 대한 인식, 특히 스토킹 처벌 의견 및 행위 구분에 있어 세대 간에 어떤 차이가 있는지 실증적으로 검증하고, 이를 통해 현행 스토킹처벌법의 개정 방향성을 도출하기 위한 자료 제공을 목적으로 한다. 이를 위해 17세 이상 한국 거주 일반인 336명을 대상으로 설문조사를 실시하였으며, 이를 바탕으로 세대 차이에 따른 스토킹 인식을 다양한 방법을 통하여 통계적으로 분석하였다. 그 결과 스토킹 처벌의견 및 스토킹에 대한 행위를 구분함에 있어 세대 간의 유의미한 차이가 있는 것으로 분석되었으며. 특히 스토킹 처벌에 있어 스토킹 피해의 실절적 대상자인 MZ세대가 강력한 대응방안을 촉구하고 있는 것으로 조사되었다. 이러한 연구결과는 향후 스토킹처벌법의 개정과 교육을 위한 기초자료 제공에 기여할 수 있을 것이라고 예상되며, 세대라는 변수를 통해 다양한 연령층의 스토킹 인식을 확인할 수 있었음에 의의가 있다.

발아메밀에서 키토산 자스몬산 처리가 페닐프로파노이드 축적에 미치는

박예은,박창하,박상언 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10

Background : Fagopyrum esculentum Moench (common buckwheat) is an important pseudocereal due to high agricultural and medicinal values. It contains various minerals, fiber, and flavonoids. Additionally, flavonoids in buckwheat have various health effects. Thus, this study is aim to optimize the concentration of chitosan, salicylic acid (SA), and jasmonic acid (JA), for the production of phenolics in germinated buckwheat using high-performance liquid chromatography (HPLC). Methods and Results : The treatment with 0.1% chitosan increased the accumulation of all 7 phenolic compounds compared with the control, 0.01 and 0.5% chitosan treatments (p < 0.05). Furthermore, the germinated buckwheat treated with JA at the specific concentrations of 50, 100, and 150 μM increased the accumulation of total phenolic compounds. The germinated buckwheat grown in 150 μM of JA showed the highest amount of total phenolics which was approximately 2.47 times higher than that of control. Particularly, the accumulation of gallic acid, rutin, catechin, chlorogenic acid, and (-)-epicatechin were approximately 2.00, 2.38, 1.76, 2.81, and 7.95 times higher in JA-treated buckwheat than in the control buckwheat samples. A total of seven phenolics, including gallic acid, catechin, chlorogenic acid, caffeic acid, (-)-epicatechin, benzoic acid, and rutin, were detected in germinated buckwheat. Apparently, JA and chitosan treatment enhanced the accumulation of phenolic compounds in the germinated buckwheat. Particularly, the treatments with 0.1 % chitosan and 150 μM JA were the most effective on the accumulation of phenolic compounds. According to the time-course analysis, a 72 h chitosan treatment enhanced the production of phenolics. Similarly, the germinated buckwheat treated with 48 and 72 h showed the accumulation of higher levels of phenolic compounds than the control buckwheat. Conclusion : This study aimed to optimize the concentrations and treatment period of elicitors, chitosan and JA, for the enhanced production of phenolic compounds in germinated buckwheat. Thus, these results might help build sturdy strategies to enhance the production of phenolics in germinated buckwheat as a good nutritional source for human consumption.

Highly flexible and compressible biowaste-derived cellulose-based carbon aerogel reinforced by 1D/2D carbon nanotubes and graphene for zinc-metal-air batteries

박예은,안성훈 한국공업화학회 2021 한국공업화학회 연구논문 초록집 Vol.2021 No.1