http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Effects of Water-Soluble Propolis on tert-Butyl Hydroperoxide-Induced Acute Oxidative Stress in Mice
김하림,Min ju Kim,Noh Eun-mi,Hur Yong Kap,Seon-Young Kim 건강기능식품미래포럼 2022 건강기능식품미래포럼 학술지 Vol.2 No.1
Propolis is a resinous substance collected by honey bees from various plant sources, that has strong anti-oxidant and anti-bacterial properties. In the present study, the two forms of water-soluble propolis extracts that were Unibee liquid propolis (LP) and Unibee powder propolis (PP) and their effects were tested in vivo. Mice were administered LP (30 mg/kg) or PP (6 mg/kg) orally once a day for five days. One hour after the administration of LP or PP on fifth day, tert-butyl hydroperoxide (t-BHP) (1.5 mmol/kg), a strong prooxidant was injected intraperitoneally to induce oxidative stress (OS). Twenty-four hours later, mice were sacrificed, weights of liver and spleen were measured, and antioxidant enzyme activities and cytokine levels were assayed from the serum. t-BHP increased the weights of liver and spleen significantly, but the increases were inhibited by pretreatments with LP and PP significantly. t-BHP decreased the activity of superoxide dismutase and level of glutathione in serum significantly, but the decreases were completely inhibited by pretreatment of PL and PP. t-BHP also increased the levels of interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-α. LP and PP inhibited the increases of IL-6 and IL-1β but not of TNF-α. These results suggest that both Unibee water soluble extracts have the activity enough to prevent the OS in vivo, and are suitable as a health functional food for the purpose of reducing OS.
Artemisia princeps Pampanini의 complete freund"s adjuvant 유발 관절염에 대한 개선 효과
김하림(Ha-Rim Kim),김솔(Sol Kim),김선영(Seon-Young Kim) 한국생명과학회 2021 생명과학회지 Vol.31 No.8
강화사자발쑥(Artemisia princeps Pampanini)은 항산화, 항염증 및 항균제와 같은 면역 기능 관련 질병에 널리 사용되는 약초이다. 이 연구에서 우리는 RAW 264.7 세포에서 AP 추출물의 항염증 효과를 조사하고 관련 메커니즘을 평가하였다. AP 추출물의 효과는 complete Freund’s adjuvant (CFA) 유도 관절염 및 lipopolysaccharide(LPS) 유도 마우스 모델에서도 평가되었다. RAW 264.7 세포에서 AP 추출물은 LPS에 의해 유도 된 산화질소(NO) 생성과 inducible NO synthase 및 cyclooxygenase-2 단백질 발현을 현저하게 억제했다. RAW 264.7 세포에서 LPS로 유도된 mitogen-activated protein kinase 와 nuclear factor-κB의 인산화 또한 AP 추출물에 의해 유의하게 억제되었다. AP 추출물의 경구 투여는 CFA 처리 마우스 그룹에 비해 발의 부종 및 비장 지수 증가를 억제하였다. 조직학적으로 CFA 처리 마우스 군에서는 cartilage와 synovium에서 염증 세포의 침윤이 증가한 반면 AP 추출물 투여군에서는 억제되었다. 더욱이, AP 추출물은 염증성 사이토카인으로 알려진 tumor necrosis factor-α 수준을 CFA 및 LPS 처리 마우스 모델에서 현저하게 감소시켰다. 결론적으로, AP 추출물의 항염증 및 항 관절염 효과는 in vitro 및 in vivo 모델 모두에서 확인되었으며, 이는 Artemisia princeps Pampanini가 관절염 치료의 후보 물질이 될 수 있음을 시사한다. Artemisia princeps Pampanini is an herbal medicine widely used to immune function-related diseases, such as anti-oxidative, anti-inflammatory, and antibacterial agents. In this study, we investigated the anti-inflammatory effects of AP extract and underlying mechanisms were evaluated in RAW 264.7 cells. The effects of AP extract were also studied in a complete Freund’s adjuvant (CFA)-induced arthritis and lipopolysaccharide (LPS)-induced inflammation mouse model. In RAW 264.7 cells, AP extracts significantly inhibited the LPS-induced nitric oxide (NO) production and inducible NO synthase and cyclooxygenase-2 protein expression. The LPS-induced phosphorylation of mitogen-activated protein kinases and nuclear factor-κB was also significantly blocked by AP extract in RAW 264.7 cells. Oral administration of AP extract suppressed the increase in mouse paw edema and spleen index compared to CFA-treated mice group. Histologically, the infiltration of inflammatory cells was increased in cartilage and synovium in the CFA-treated mouse group, whereas it was suppressed in the AP extract-administered group. Furthermore, AP extract treatment significantly reduced the inflammatory cytokine, tumor necrosis factor-α, levels in CFA and LPS-treated mouse. In conclusion, the anti-inflammatory and anti-arthritis effect of AP extract was confirmed in both in vitro and in vivo models, suggesting that Artemisia princeps Pampanini may be a candidate material for arthritis treatment.
방향족 무수물 기반의 에폭시 변성 아크릴레이트 바인더 합성 및 SiO₂ 레지스트 제조에 관한 연구
김하림(Ha-Rim Kim),김도현(Do-Hyun Kim),김영운(Young-Woon Kim),이운영(Woon-Young Lee),배진영(Jin-Young Bae) 한국고분자학회 2018 폴리머 Vol.42 No.4
본 연구에서는 유기 절연막에 적용 가능한 새로운 광경화 에폭시 변성 아크릴레이트의 합성 및 그 특성에 대해 살펴보았다. 우선 다양한 에폭시 변성 아크릴레이트를 합성하기 위하여 산 무수물과 하이드록시 아크릴 단량체의 조합에 의해 diesteric acid(DA) 유도체를 반응시켰다. 합성된 diesteric acid(DA)에 에폭시 화합물인 글리시딜 메타아크릴레이트(glycidyl methacrylate)를 반응시켜 광경화 타입의 에폭시 변성 아크릴레이트를 합성하였다. 그리고 실리카 분산액인 NANOBYK-3650과의 호환성을 살펴보았다. 사용한 단량체의 구조에 기인하여 실리카와 최적의 바인더를 선정하고 이를 고투과율·저유전 실리카 레지스트에 적용시키고자 배합을 하여 유기 절연막을 제조하였다. 실리카 함량에 따른 투과율, 패턴성, 열 안정성 그리고 전기적 특성(유전 상수)을 확인하였다. In this study noble UV-curable epoxy modified acrylates binder was designed and synthesized to apply an organic insulating film. Firstly, diesteric acid (DA) derivatives were prepared from anhydrides and hydroxy acrylates to synthesize epoxy modified acrylates. UV-curable epoxy modified acrylates were prepared from synthesized diesteric acid (DA) and epoxy chemicals, glycidyl methacrylate (GMA). The miscibility with silica millbase ‘NANOBYK-3650’ was investigated. Appropriate binder with silica was chosen by considering the monomer chemical structure. To obtain an organic insulating film with high transmittance and low k silica resist, optimum composition of UV-curable epoxy modified acrylates was also formulated. Transmittance, patternability, and electrical properties (dielectric constant) was measured.
이다영,김남석,김하림,김민선,이창현,오미진,오찬호,김종석,김명순,권진,Lee, Da young,Kim, Nam Seok,Kim, Ha Rim,Kim, Min Sun,Lee, Chang Hyun,Oh, Mi Jin,Oh, Chan Ho,Kim, Jong Suk,Kim, Myung Soon,Kwon, Jin 한국식품영양학회 2018 韓國食品營養學會誌 Vol.31 No.2
This study was investigating the anti-obesity effects of diets with a mixture of cheese, red ginseng and fermented mature fruit of Rubus coreanus Miquel. We fed the experimental diets (SC5, SC10) supplemented with 5% or 10% cheese containing of fermented mixture, then orally administered the extract of fermented mixture (RB100, RB300) at a concentration of 100 or 300 mg/kg body weight to SD rats with high fat diet (HFD). The results were as follows: Sample cheese groups decreased visceral fat mass and adipocyte size of stomach compared to that of HFD group. Additionally, lipid droplets of liver in sample cheese groups were smaller than that in HFD group. The serum triglyceride (TG), total cholesterol (tChol), glucose, leptin, and insulin levels in sample cheese group were lower than that in HFD group. But, the serum adiponectin and HDL-cholesterol (cHDL) levels were higher than that in HFD group. These results suggest that fermentation of red ginseng and Rubus coreanus mixed with cheese might be helpful in preventing obesity in high fat diet-fed rats.