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      • 소 腎臟 Adenosine Deaminase Complexing Protein의 精製와 生物學的 性狀에 關한 硏究

        김철호 인제대학교 1992 仁濟醫學 Vol.13 No.4

        소의 신장 adenosine deaminase complexing protein을 순수 정제하였으며, 생물학적 성상에 관한 연구를 시도하였다. 그 결과 adenosine deaminase complexing protein은 adenosine의 농도에 따라 adenosine deaminase의 활성을 조절하는 역할을 할 것이라 사료된다. ADCP (adenosine deaminase complexing protein) was prepared and purified from bovine kidney. Its purification was done by the treatment of Triton X-100, followed by precipitation with sodium sulfate, ADA-Sepharose affinity chromatography and finally Sephadex G-100 gel filteration. The purified ADCP thus obtained is supposed to exist as a homodimer of molecular weight about 200 KD on SDS-polyacrylamide gel electrophoresis. The presence of ADCP caused a slight decrease in Km and Vmax value for adenosine, but the ADA (adenosine deaminase) binding with ADCP did not modify its behavior of optimal pH and temperature. No effects of varying metal ions, purine metabolites and its derivatives were observed on the activities of ADA in the presence of ADCP, except 6-chloropurine riboside inducing 27% decrease in activity and Fe2+ enhancing 29% of its activity. The binding of ADA with ADCP was optical at 1.7 : 1 molar ratio of ADA/ADCP with no effect of the addition of varying metal ions or purine metabolites except the guanosine and 2,6-diaminopurine, both causing aggravating complex with their concentration. The effect of ADCP on catalytic activity of ADA was studied. The slight elevation (up to 13%) of ADA catalytic activity was observed at the adenosine contents of 0.1∼0.2 mM and ADCP/ADA molar ratio of 1 : 1∼1 : 10, while the decrease in its activity was shown at the same range of adenosine contents, but ADCP/ADA molar ratio of 2 : 1∼10 : 1. The lower activities of ABA were also observed at lesser contents (0.01∼0.05 mM) of adenosine and the same molar ratio of ADCP/ADA. Thus it was shown that ADCP interacted with ADA. And the ADA activity tends to decrease at the physiological concentration of adenosine (about 0.01 mM). These results show that ADCP might play a role in the regulation of ADA activity.

      • KCI등재

        電子商去來와 關聯한 컴퓨터 네트워크上의 紛爭事例硏究

        金喆浩 국제지역학회 1998 국제지역연구 Vol.2 No.2

        By increasing a computer network, internet usage, it has been created for Electronic Commerce to place a cyberspace. This cyberspace is limited to apply for contemporary law and custom because it have distinct characters now. Therefore, it is predicted that many disputes will be happened, in these cyberspaces. It is difficult to find a involved case in Korea, but we can find it easily in U. S. A. This article examines these cases happened in the network and studies a reasonable improvement ways. It has been limited to apply the law and custom the network which can be happened a dispute and has not involved law and custom using computer network yet. Therefore, we should make a self-regulation each other who use network and improved the law and custom involved the network through these methods. We should also establish Alternative Dispute Resolution and try to settle dispute in the cyberspace.

      • 利益變動原因分析의 모델

        金喆鎬 弘益大學校 1982 弘大論叢 Vol.14 No.1

        This paper provides some tools to analyze variation factors is a firm's normal profit. For the purpose of developing these, this abstract begins with the analysis of variation causes in sales, cost of goods sold, operating expenses and the other revenues. and expenses, and finally concentrates on the correlation analysis of cost-volume-profit. 1. Analysis of variation in sales Increase of market price & decrease of quantity: ΔS_a=(P'-P)Q'+P(Q'-Q)={P(1+r₁)-P}Q'+P{Q(1-r₂)-Q} Decrease of market price & increase of quantity: ΔS_b=(P'-P)Q+P'(Q'-Q)={P(1-r₁)-P}Q+P'{Q(1+r₂)-Q} Increase (or Decrease) of market price & quantity: ΔS_c={(P'-P)Q+½(P'-P)(Q'-Q)}+{P(Q'-Q)+½(P'-P)(Q'-Q)}={[P(l±r₂)-P)Q+½P(1±r₁)-P)][Q(l±r₂)-Q]}+{P[Q(1±r₂)-Q)+½P(1±r₁)-P][Q(l±r₂)-Q]} (S:sales, P:previous market price, P':current market price Q:previous. quantity, Q':current quantity, r₁:rate of variation in price, r₂:rate of variation in quantity) 2. Analysis of variation in CGS. Increase in CGS per unit & decrease in quantity: ΔC_(pa)=(p'-p)Q'+p(Q'-Q) Decrease in CGS per unit & increase in quantity: ΔC_(pb)= (p'-p)Q+p'(Q'-Q) Increase (or Decrease) in CGS per unit & quantity: ΔC_(pa)={(p'-p)Q+½(p'-p)(Q'-Q)}+{p(Q'-Q)+½(p'-p)(Q'-Q)} (p : previous CGS per unit, p' : current CGS per unit) 3. Analysis of variation in operating expenses Increase in the rate of operating variable expenses per unit &decrease in quantity: ΔC_(o₁)=(f_0'-f_0)+(p'·r₄'-p·r₄)Q'+p·r₄(Q'-Q) Decrease in the rate of operating variable expenses per unit & increase in quantity: ΔC_(o₂)=(f_0'-f_0)+(p'·r₄'-p·r₄)Q+p'·r₄'(Q'-Q) Increase (or Decrease) ,in the .rate of operating variable expenses per unit & quantity: ΔC_(o₃)=(f_0'-f_0)+{(p'·r₄'-p·r₄)Q+½p'·r₄'-p·r₄)(Q'-Q)}+{p·r₄(Q'-Q)+½p'·r₄-p·r₄)(Q'-Q)} (f_0 : previous operating fixed expenses, f_0' : current operating fixed expenses, r₄ : previous rate of operating variable expenses, r₄' : current rate of operating variable expenses) 4. Analysis of variation in the other revenues & expenses. Other revenues: ΔN₁=(I₁'-I₁)+(A₁'-A₁)+(M₁'-M₁) Other expenses: ΔN₂=(I₂'-I₂)+(D'-D)+(A₂-A₂)+(M₂'-M₂) (I₁ : receivable interest, I₂ : payable interest, A₁ : valuation income, A₂ : valuation expense, D : depreciation, M₁ : miscellaneous income, M₂ : miscellaneous expenses) :5. Analysis of the correlation of cost-volume-profit. Gross Margin (G₁) : ΔG₁=ΔS-ΔC Operation Margin (G₂) : ΔG₂=ΔS-(ΔC+ΔC_0) Normal profit (Earning before special gains or losses & income taxes)(G₃) : ΔG₃=(ΔS+ΔN₁)-(ΔC+ΔC_0+ΔN₂) (N₁ : non-operating income, N₂:non-operating expenses) 'Since the ultimate purpose of analysis of variation in profit is to provide some informations for management, the further analysis for each product, region & customer in sales has to be included in the study on the variation in margin. Therefore, I think,. a more careful and sufficient study on this field should be forthcoming.

      • KCI등재

        B2C Cross-border e-Commerce관련 법규범에 관한 연구

        김철호 한국무역통상학회 2018 무역통상학회지 Vol.18 No.4

        Recently, the B2C cross-border e-commerce has been rapidly increasing. According to this trend, the laws and regulations related B2C cross-border e-commerce should be prepared to clarify the concepts and areas between e-trade and B2C cross-border e-commerce. First, B2C cross-border e-commerce laws and regulations should be prepared to improve the laws related to B2C cross-border e-commerce. Second, it is urgent to unify the terms related to B2C cross-border e-commerce, which is used variously in relation to B2C cross-border e-commerce. Third, it is necessary to revise various support systems for e-commerce and e-trade infrastructure according to the characteristics of B2C cross-border e-commerce. Fourth, B2C cross-border e-commerce professional support center and committee for dispute settlement are necessary. Also, in order to efficiently support and manage B2C cross-border e-commerce, it is necessary to unify management institutions.

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