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김창옥,성영미 이화여자대학교 의과대학 1984 梨花醫學誌 Vol.- No.16
Four hundred and three patients with sputue cytologic examination were presented at Ewha womens University Hospital during the period of 3 years from 1981 to 1983. 1) Age distribution was highest in the age of 60 years, 28.9% and the ratio of male to female in the patients was 1.7 : 1. 2) Among the whole 366 cases differentiated by the Pap. class, the class Ⅰ was present in 190 cases (51.9%) class Ⅱ in 123 cases (33.6%), and the class Ⅴ in 38 cases (10.4%). 3) The ratio of make to female according to Pap. class were 114 : 71 in the class Ⅰ. 71 : 51 in the class Ⅱ, 11 : 20 in the class Ⅲ, 3 : 1 in the class Ⅳ. and 28 : 8 in the class Ⅴ. 4) In the Pap. class Ⅳ. Ⅴ cases, percentage of positivity to examined number of sputum cytology was highest in the area from 91% to 100%, as 11 cases (26.2%), but the distribution covered a wide area from 11% to 80%. 5) Among the 403 cases examined by sputum cytology, class Ⅳ. Ⅴ were 42 cases, and their positivity in 4th successive sample was 35 cases (90.4%). 6) Of 34 cases dianosed by the histologic typing, the highest case was spuamous carcinome as 17 cases(50%), Adeno ca. was 7 cases (20.6%) 1 cases(2.9%). 7) In the ratio of male to female histologic typing, that of squamous carcinoma was 14 : 3 with male preponderance. That of adenocarcinoma was 3 : 4 and that of oat cell carcinoma was 8 : 1. 8) In the percentage of positivity to examined number according to histologic type, the positivity of oat cell carcinoma was high, that of adenocarcinoma was low, but that of squamous carcinoma covered a wide area. 9) In positive cases in number according to type of cancer, initial positive number of squamous carcinoma covered from 1st to 9th, that of oat cell carcinoma finished at 2nd. In summery the positivity of the sputum was lower over the 5th sputum samples but it is thought that sputum examination after 5th sputum samples were needed according to individuals. Sputum cytology was easier than other method, and has important role in the diagnosis of the lung cancer.
김창옥 대한기계학회 1991 대한기계학회논문집 Vol.15 No.3
본 연구에서는 균일압축력을 받으며 네변이 단순지지된 비균일 두께를 가진 평판의 좌굴응력을 Rayleigh-Ritz방법으로 해석하여 평판두께를 적절히 분포함으로써 동일한 평판중량으로 더 큰 좌굴하중을 얻을 수 있음을 보이며 또한 동일한 문제를 NASTRAN프로그램을 사용하여 해석함으로써 그 정확도를 입증한다.
실험적으로 유발된 랫드의 뇌종양 모델에 관한 병리학적 연구(Ⅱ)
김창옥,김기석,김대중,안병우,한범석,홍석민,장우영,정상우,천명훈,최광식 식품의약품안전청 1997 식품의약품안전청 연보 Vol.1 No.-
신경교종의 로질에 있어서 세포증식능을 알아보기 위한 하나의 밟법으로서 증식세포 핵항뭔(PCNA) 단택과 bromodeoxy uridine(BrdU)단백의 발현양상을 관찰함으로써 항암제드으이 효과를 알 수 있는 지표로서 연구되어져 왔으며, 종양 발생의 기전과 유전자 치료법 개발 등의 연구를 위해 뇌종양과 관련되는 종양유전자 변화등이 연구되어져 왔다. 따라서 본 연구에서는 전년도에 확립된 9L 세포주를 이용한 랫드의 뇌신경교종모질을 이뭉하여 PCNA와 BrdU의 발현양상과 P53 단백, c-erbB-2 유전자 산물등의 종양단백의 유무를 알아보고자 하였다. 9L 세포준를 8주령된 즐D랫드의 미상핵 부위에 직접 투여한 후 2주후에 관류고정 후 부검을 씨행하였다. PCNA와 BrdU 단백에 대한 면역염색 후 영상분석기를 이용하여 면역염색된 면적을 측정하엿으며 labeling index인 전체 세포수중에서 면역 염색된 세포수의 백분율을 측정하였다. PCNA 단백에 대한 면역조직화학적 염색 소견상 종양전반에 비교적 균일하게 염색되었으며 염색되는 양상은 방추형의 세포의 핵에 염색되었으며, Brdu는 종양의 주변부케 국한하여 세포의 핵에 약양성으로 염색되었다. c-erCB-2는 국소적으로 염색되었으며 염색되는 양·탕은 세포막을 따라 염색되었다. P53 단백은 국소적으로 염색되었으며 종양세포의 핵에 염색되는 양상을 보였다. PCNA에 대해 면역 염색된 면적은 전체 면적에 대하여 평균 5.26% 염색 면적이 측정되었다. 전체 Pixels수인 768×512개로 환산해 보면 평균 393216pixe1s· 개수가 염색뵉었으며 PCNA labeling index는 48%였다. 이상의 결과로 세포증식능의 지표로서 종양전반에 걸펴 비 교적 균일하게 염색되고 labeling index도 대조군으로 적당한 48%정도를 보이는 PCNA글 적합하며 면역조직화학 염색상 P53단백과 c-erbB-2의 유전자 산물에 대한 국소적 양성 반응을 확인하였다. ' Expression of PCNA protein and BrdU protein which is a index of anticancer drug of(ect is a methods to verify cell proliferation in glioma model, and changes of oncogene concerning to braintumor has studied to identify mechanism of turner initiation and to develope gene therapy method- Thisstudy was performed to identify the expression of PCNA, BrdU, P53, c-erbB-2 gene product proteinusing rat brain tumor model induced by 9L ce31 line. After 9L cell lirte was implantated to nucleus partof 8 week-old SD rats, sacrificed them 2 weeks later. After immunohistochmical staining of PCNA andBrdU proteiu, stained cell area and percentage of immunostained cell in total cell, that is 1?beling index,was measured. Stained pattern of PCNA protein was evenly distrihuted to tumor cell and focused on no-cleolus of spindle cell. That of BrdU was distributed to peripheral f)art of tumor and nucleus showedweak positive. c-erbB-2 was focaB along the cytoplasmic membrane and PS3 protein was focal on nucle-us of tumor cell. PCNA stained area was 5.26% of total area. Mean pixel number was 393216, so PCNAlabeling index was 48%. This results indicate that PCNA was most adequate as proliferating index, forit was evefly distribute and it's labeling index showed 48%, and imnlunohistochemical staining of P53protein af'a c- erb8-2 gene product showed focal positive reaction.