고려인삼의 Cytochrome P450 및 P-Glycoprotein 매개 약물상호작용에 미치는 영향

남기열,양병욱,신왕수,박종대 한국생약학회 2018 생약학회지 Vol.49 No.2

A drug interaction is a situation in which a substance affects the activity of a drug, synergistically or antagonistically, when both are administered together. It has been shown that orally taken ginsenosides are deglycosylated by intestinal bacteria to give ginsenosides metabolites, which has been considered to be genuine pharmacological constituents and to exhibit drug interactions. Animal experimental results demonstrated that ginsenoside metabolites play an important role in the inhibitory or inductive action of both CYPs (cytochrome p450) and P-gp (p-glycoprotein), thereby can be applied as metabolic modulator to drug interactions. Very few are known on the possibility of drug interaction if taken the recommended dose of ginseng, but it has been found to act as CYPs inductor and P-gp inhibitor in any clinical trial, suggesting the risk that side effects will occur. It has been recently reported that interactions might also exist between ginseng and drugs such as warfarin, phenelzine, imatinib and raltegravir. Moreover, medicinal plants are increasingly being taken in a manner more often associated with prescription medicines. Therefore, considering the extensive applications of ginseng for safety, the aim of this review is to present a comprehensive overview of ginseng and drug interactions based upon pharmacodynamic and pharmacokinetic evidences.

Protein Phosphatase 2A의 활성화에 미치는 Lipid Bilayer Membrane의 저해 효과

남기열 한국생물공학회 1992 KSBB Journal Vol.24 No.2

protein phosphatase 2A는 bovine brain homogenate의 세포질 fraction에서 얻어졌다. 기질로서 인산화된 histione H1을 이용하여 측정한 phosphatase 의 활성은 dipalmitoyIphophatidylcholine(DPPC) 혹은 phosphatidylserine/DPPC의 혼합물로 구성된 liposome의 존재하에서 저해되었다. Protein phosphatase 2A의 lipid membrane에의 결합은 다중층 지질막의 혼합물 계에서 liposome 의 양이 증가함에 따라서 상등액 중의 phosphatase의 활성이 감소하는 것으로 확인할 수 있었다. 또한 [$^{125}I$]protein phosphatase 2A가 liposome과 동시에 용출되는 것으로도 확인되었다. 그러나 liposome에 대한 protein phosphatase의 친화력은 높지 않았다. 한편, okadaic acid와 liposome은 협동으로 phosphatase의 활성을 감소시켰다. 이것은 okadaic acid가 lipid membrane이나 membrane에 결함한 phosphatase에는 결합하지 않는다는 것을 의미한다. 그러므로 lipid membrane에 의한 protein phosphatase 2A의 활성 저해 효과는 phosphatase 2A와 lipid membrane과의 결합에 의한 것이라고 설명될 수있다. Protein phosphatase 2A was obtained from a cytosolic fraction of bovine brain homogenate. The phosphatase activity using phosphorylated histone Hl as substrate was suppressed in the presence of liposomes composed of dipalmitoylphosphatidylcholine(DPPC) or the mixture of phosphatidylserine and DPPC. The binding of protein phosphatase to liposome was indicated by the facts that the phosphatase activity of the supernatant of protein phosphatase/multilayer vesicle mixture was decreased with increasing amount of liposome, and that [$^{125}I$]-labeled protein phosphatase was coeluted with liposome. However, the affinity of the protein for phospholipid membrane was not so high. On the other hand, okadaic acid and liposome reduced the phosphatase activity synergistically, which means that okadaic acid binds neither to lipid membrane nor to the membrane-associated phosphatase, The inhibitory effect of liposome was, therefore, ascribed to association of the protein phosphatase 2A with the lipid bilayer membrane.

인삼의 임간재배가 생육 및 품질에 미치는 영향

남기열,손석룡,배효원,Nam, Gi-Yeol,Son, Seok-Ryong,Bae, Hyo-Won 고려인삼학회 1980 Journal of Ginseng Research Vol.4 No.1

In order to increase the production of ginseng, a cultural experiment was carried out under different types of natural forest condition. Seedlings were transplanted with three spacing (70,90 and plants per 1.62m2) under the broad leaved, needle and mixed forest. The obtained results are as follows. 1 Growth of aerial part of ginseng plant. 1) Vegetative growth under forest condition of very poor as compared with ordinary cultivation, but there was no significant in number of leaf and teasel. 2) Stem diameter and stem length under the different forest types were a little difference. However petiole length and number of leafet showed an increasing trend in broad leaved forest as compared with other forest types. 3) The withering date of aerial part of ginseng plant in the needle forest was later than of others. 2. Fresh weight of ginseng root per plant was decreasing in the order of broad leaved forest, needle and mixed forest, needle and sized forest respectively. However the root weight was much smaller than that of ordinary cultivated one. 3. No big difference was observed in the growth of both aerial and root among the planting density 4. Nitrogen content in ginseng root under forest was lower, but calcium content In root was higher than that of ordinary one. 5. Fat and fiber content of ginseng root under forest showed higher than that of ordinary one. 6. The saponin content of ginseng root grown under forest condition was higher than that of ordinary ginseng root. According to high performance liquid chromatogram of saponin, only difference from ordinary cultivated ginseng root was that ginsenoside Re showed higher peak than ginsenoside Rg1.

인삼의 한의학적 및 현대임상적 측면에서의 복용량 검토 ( Usage and Dosage of Ginseng Radix ( Panax ginseng C. A. Meyer ) Based upon Traditional and Recent Scientific Clinical Applications )

남기열,박종대 고려인삼학회 2000 Journal of Ginseng Research Vol.24 No.2

인삼의 임간재배가 생육 및 품질에 미치는 영향

남기열 충북대학 대학원 1980 大學院論文集 Vol.6 No.-

본문참고

인삼의 임간재배가 생육 및 품질에 미치는 영향 ( Effect of Cultivation under Forest on the Growth and Quality of Ginseng )

남기열,손석용,배효원 고려인삼학회 1980 Journal of Ginseng Research Vol.4 No.1

인삼의 품질과 약리활성 물질과의 상관성 ( Relationship of Saponin and Non-saponin for the Quality of Ginseng )

남기열,고성룡,최강주 고려인삼학회 1998 Journal of Ginseng Research Vol.22 No.4

모듈조립공정 운영개선을 위한 시뮬레이션 모델링

남기열 대한산업공학회 2015 대한산업공학회 춘계학술대회논문집 Vol.2015 No.4

본 연구는 휴대폰, OLED 모듈, TFT-LCD 모듈 등의 모듈조립공정에 대한 시뮬레이션 모델을 제시하였다. 본 시뮬레이션 모델은 모듈조립공정에서 생산성에 영향을 미치는 설비고장, 작업자 휴지시간, 자재공급방식, 양품율, 버퍼 사이즈, 로트 사이즈, 공정내 물류방식 등 다양한 요소를 반영하였다. 재공배수 최소화를 위해 공정내의 운영 파합지터값인 투입량, 합지버퍼, 합지로트 사이즈과 여러 공정 운영 대안들에 대한 평가결과를 제공한다. 최선의 개선대안 선택을 위해 ARENA의 OptTek Systems사의 OptQuest라는 소프트웨어 패키지를 사용하였다. 시뮬레이션 모델링을 통해 공정의 문제점을 파악하였고, 재공배수의 최소화를 위한 최선의 투입량, 합지버퍼, 합지로트 사이즈를 결정하여 기존공정대비 생산량 5.8%증가, 재공 90%감소, 재공배수 90% 감소 결과를 도출하였다.

도료 적용을 위한 생체고분자의 이론

남기열 한국공업화학회 1997 한국공업화학회 심포지엄 Vol.0 No.-

고려홍삼의 주요 성분과 효능

남기열,양덕춘 한국자원식물학회 2003 한국자원식물학회 학술심포지엄 Vol.2003 No.-

Fruit ripening represents a genetically synchronized system that involves developmental process unique to plant species, The phenomenon of ripening includes changes in color, texture, respiration rate, flavor, and aroma. Ripe fruits generally exhibit increased susceptibility to pathogen infection. However, fruits as a reproductive organ have their own protection mechanism against pathogens to maintain their integrity during seed maturation. In several nonclimacteric fruits, such as cherry, grape, and pepper, that do not have an ethylene burst during ripening, resistance against phytopathogens increases during ripening. Colletotrichum gloeosporioides is a causal agent of anthracnose disease in pepper plants (Capsicum annuum). We have established that C. gloeosporioides has susceptible and resistant interactions with pepper fruits during pre- and post-ripening stages, respectively. And we have interested in looking for a molecular mechanism that would explain the fungal resistance during ripening of nonclimacteric pepper fruit. In this presentation, a molecular characterization of the pepper esterase gene (PepEST) that is highly expressed in the resistant response will be demonstrated as an example of development and industrial applications of versatile-usable genes of plant.