다양한 생물반응기 형태가 인삼(Panax ginseng C.A. Meyer) 부정근의 생장과 Ginsenoside 생산에 미치는 영향

김윤수,한은주,백기엽,Kim, Yun-Soo,Hahn, Eun-Joo,Paek, Kee-Yoeup 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.3

생물반응기의 형태에 따른 인삼부정근의 생장과 ginsen-side의 생산능력에 대하여 조사한 결과, 원형의 상부와 하부에 5 cm의 bubble column을 가진 bulb type bubble bioreactor (BU)에서 건물중은 41.92 g으로 가장 많이 증가하였으며, cylindric tube bioreactor (CT)에서 건물중이 38.55g으로 가장 낮게 나타났다. 이들 두 생물반응기의 초기 kLa 값은 BU 생물반응기에서 6.98 h$^{-1}$로 가장 높게 측정되었고, 반대로 bubble column이 없는 CT 생물반응기에서 5.25 h$^{-1}$으로 가장 낮게 측정되었다. 그러나 이와 같은 초기 kLa값의 차이는 부정근내의 이차대사산물인 ginsenoside의 함량에는 영향을 주지 않는 것으로 나타났다. 또한 BU 생물반응기에서 bubble column의 길이를 기존의 5 cm에서 10 cm로 연장시켰을 때, 초기 kLa값이 6.52 h$^{-1}$에서 7.80 h$^{-1}$로 증가하면서 인삼부정근의 생장을 42.13 g에서 50.30 g으로 약 16% 증가시킨 것으로 나타났다. The type of air lift bioreactor affected the root growth in ginseng adventitious root cultures. Among bioreactors used in this experiment, bulb type bubble bioreactor (BU) was the best to increase root growth (41.92 g dry weight). The kLa value representing the oxygen transfer capacity from medium to explants (6.98 h$^{-1}$ ) in BU with 5 cm bubble column was higher than other bioreactors. On the other hand, cylindric tube bioreactor (CT) without bubble column resulted in minimum root growth (38.55 g dry weight) and kLa value (5.25 h$^{-1}$ ). Furthermore, the root growth (50.30 g dry weight) in BU with 10 cm bubble column more increased than 5 cm bubble column. However, the kLa value do not affected the secondary metabolite such as ginsenosides. These results show that the bubble column in air lift bioreactor increase kLa value and increased kLa value stimulate the growth of ginseng adventitious roots.

남아선호사상에 기반한 출생 성비에 관한 확률론적 고찰

김윤수,최은선,차경준,Kim, Yun-Soo,Choi, Eun-Sun,Cha, Kyung-Joon 한국수학사학회 2008 Journal for history of mathematics Vol.21 No.4

우리나라의 출생 성비는 자연 상태에서의 출생 성비(natural sex ratio at birth)로 추정되는 105를 초과하고 있는데, 그 원인 중 하나로 남아선호사상으로 인한 인위적인 출산이 있다(e.g. 임신중절 등). 본 연구에서는 임신 중절 없이 남아를 낳을 때까지만 출산을 하려는 가정(family)들의 존재가 출생 성비를 높이는 데에 기여하는지를 알아보기 위하여, 이러한 가정들과 출생 성비(sex ratio at birth)의 관계를 분석하였다. 그 결과, 남아 한 명을 낳을 때까지만 출산을 하려는 가정의 수가 무한으로 발산할수록 출생 성비는 자연 상태에서의 출생 성비에 확률적으로 수렴하는 것을 알 수 있었다. 즉, 남아를 낳을 때까지만 출산을 하려는 가정들의 존재는 출생 성비에 영향을 미치지 않음을 확인하였다. The sex ratio at birth of South Korea is exceeding the natural sex ratio at birth, which is estimated to be about 105. One of the reasons of high sex ratio at birth is due to sex-selective abortion which is caused by strong son preference. The main objective of this study is to identify whether the families which are trying to bear children only until they acquire one son contribute to high sex ratio at birth. As a result, we obtain the theorem that if the number of such families diverge, the sex ratio at birth converges to the natural sex ratio almost surely. Therefore, we conclude that the existence of the families which are trying to bear children only until they receive one son does not affect the sex ratio at birth.

인삼의 우수재배관리 기준 이행을 위한 주요관리점

김윤수,이병철,조병구,서지호,배봉석,Kim, Yun-Soo,Lee, Byung-Chul,Cho, Byung-Goo,Seo, Jiho,Bae, Bong-Seok 고려인삼학회 2019 고려인삼연구와 산업 Vol.13 No.1

IBA와 NAA 처리에 의해 생성된 Ethylene이 인삼(Panax ginseng C.A. Meyer) 부정근의 생장과 발달에 미치는 영향

김윤수,한은주,백기엽,Kim, Yun-Soo,Hahn, Eun-Joo,Paek, Kee-Yoeup 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2

The effect of IBA and NAA on adventitious root cultures of Panax ginseng C.A. Mater were investigated. Results indicated differences in growth and development of the roots according to 5mg/L IBA and 2mg/L NAA. IBA resulted in a normal root development and a higher growth compared to NAA. The roots formed on NAA-containing media were shorter and thicker than those in IBA, showing a hypertrophy of the root tip. NAA induced more than 1.6 times higher ethylene production compared to IBA, which caused inhibition of the root growth. Under the ventilation, in the other hand, on difference was observed in ethylene concentration and the root growth between IBA and NAA treatments. Under ventilation ethylene production was not detected until 10 days of culture, while detected from the initial stage under on ventilation. The results suggested the importance of ventilation during the culture for the growth and development of ginseng adventitious roots.

환경적 footprint 분석을 통한 토양경작법과 화학적산화법의 비교

김윤수,임형석,박재우,Kim, Yun-Soo,Lim, Hyung-Suk,Park, Jae-Woo 한국지하수토양환경학회 2015 지하수토양환경 Vol.20 No.3

In this study, land farming and chemical oxidation of a diesel-contaminated site is compared to evaluate the environmental impact during soil remediation using the Spreadsheet for Environmental Footprint Analysis by U.S. EPA. Each remediation process is divided into four phases, consisting of soil excavation, backfill and transportation (Phase 0), construction of remediation facility (Phase 1), remediation operation (Phase 2), and restoration of site and waste disposal (Phase 3). Environmental footprints, such as material use, energy consumption, air emission, water use and waste generation, are analyzed to find the way to minimize the environmental impact. In material use and waste generation, land farming has more environmental effect than chemical oxidation due to the concrete and backfill material used to construct land farming facility in Phase 1. Also, in energy use, land farming use about six times more energy than chemical oxidation because of cement production and fuel use of heavy machinery, such as backhoe and truck. However, carbon dioxide, commonly considered as important factor of environmental impact due to global warming effect, is emitted more in chemical oxidation because of hydrogen peroxide production. Water use of chemical oxidation is also 2.1 times higher than land farming.

생물반응기내의 공기주입량 및 Sparger 형태가 인삼 (Panax ginseng C.A. Meyer) 부정근의 생장과 Ginsenoside 함량에 미치는 영향

김윤수,한은주,신차균,백기엽,Kim Yun-Soo,Hahn Eun-Joo,Shin Cha-Gyun,Paek Kee-Yoeup 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.2

The two different ways to supply air inside the bioreactor were examined in the adventitious root cultures of Panax ginseng C.A. Meyer. First, the aeration rate varied at 0.05, 0.1, 0.2 and 0.3 vvm, respectively which were supplied during the whole culture period. Second, the amount of air supply was increased from 0.05 to 0.3 vvm at 10-day intervals in proportion to the root growth. Both the root growth and the ginsenoside accumulation were maximized to 175.8 g dry wt. of root growth and 4.3 mg/g dry wt. of ginsenoside accumulation when the aeration rate was increased gradually. The effect of the sparger pore size (15, 30 and $60\;{\mu}m$) in the bioreactor was also investigated, which suggested the greatest root growth (175.9 g dry wt.) in the $15{\mu}m$-sized sparger and the highest ginsenoside content (4.3 mg/g dry wt.) in the $60\;{\mu}m$ size. Finally, the diameter of a sparger ($15\;{\mu}m$-sized) varied at 1.5, 3.0, 5.0 and 8.0 cm, respectively. The highest root growth (191.9 g dry wt.) and the ginsenoside content (4.9 mg/g dry wt.) were obtained in the sparger diameter of 8.0 cm. 생물반응기 배양을 통하여 인삼 부정근을 대량생산하고자 할 때, 가장 이상적인 공기주입량의 조절은 배양초기부터 말기까지 각각의 농도 (0.05, 0.1, 0.2, 0.3 vvm)를 가지고 동일하게 주입하는 방법보다는 인삼 부정근의 생장이 증가함에 따라 공기주입량을 약 10일 간격을 두고 $0.05{\sim}0.3\;vvm$으로 서서히 증가시키는 것이 인삼 부정근의 생장(175.8 g dry wt)과 총 ginsenoside 함량 (4.3 mg/g dry wt)을 최대로 증가시켰다. 또한 생물반응기내 sparger의 다공 크기를 15, 30, $60\;{\mu}m$으로 각각 제작하여 배양하였을 경우, 인삼 부정근의 생장은 $15\;{\mu}m$ sparger (175.9 g dry wt)에서 양호하였으나 총 ginsenoside 함량은 $60\;{\mu}m$ sparger (4.9 mg/g dry wt)에서 우수하게 나타났다. 마지막으로 sparger의 직경을 1.5, 3.0, 5.0, 8.0 cm로 각각 제작하여 배양하였을 때 (공기주입량은 $0.05{\sim}0.3$ vvm, sparger의 다공크기는 $15\;{\mu}m$로 채택), 8.0 cm sparger에서 인삼 부정근의 생장(191.9 g dry wt)과 총 ginsenoside 함량 (4.9 mg/g dry wt)이 다른 처리구에 비하여 가장 우수하게 나타났다.

인문사회학편 : 우수 사관생도 선발을 위한 입학제도 개선방안 연구 -입학사정관계의 도입 검토를 중심으로-

김윤수(경제학박사) ( Yun Soo Kim ),임도한(교수) ( Do Han Lim ),김득수(부교수) ( Deuk Su Kim ),장영일(부교수) ( Young-il Jang ),윤유경(교수) ( You Kyung Yoon ),장성화(조교수) ( Sung Hwa Jang ),김기둥(조교수) ( Ki Doong Kim ) 공군사관학교 2015 空士論文集 Vol.66 No.2

미래 국방환경의 연화는 사관학교의 경쟁력 제고를 요구하고 있다. 경쟁력 세고는 인적 자원의 질 문재와 직결되으로 무엇보다도 우수한 인재를 선발하는 문제가 중요하다고 할 수 있다. 이를 위 하여 본 연구에서는 민간대학에서 시행중인 입학사정관제를 분석하여 도입방안을 검토하였다. 입학사정관제에서는 학교가 원하는 인재상을 설정한 뒤 해당 가치를 근거로 하여 인재 선발을 하고 있으며 정성적 평가의 객관성 확보를 위하여 구체적인 지표를 마련하고 있음을 확인하였다. 이에 따라 본 연구에서는 국정과제 및 공군버전, 컨설팅 결과 등을 참조하여 미래 국방환경이 요구 하는 사관학교 인재상을 설정한 뒤, 해당 역량의 구체적 평가전형 요소를 개발하였다. The competitiveness of military academies must De improved in accordance with changes in the future environment of national defense. Since this improvement will be directly connected lO the Quality of military hwnan resources, it is paramount that talented cadets be admitted to the academies. This study analYzed the admission officer system of civilian universities. investigating its application to the military academies. In the admission officer system. universities first define characteristics of their target model and select prospectice students accordingly. We confirmed that the universities established a detailed index of characteristics to ensure objectivity in lheir Q.ualitative evaluations. Wilh reference to the current national policies. the ROK Air Force visions. and the professional consultation results of the ROK Air Force Academy, we defined characteristics of the Academy larget model and developed specific evaluation indicators for these characteristics.

전단 파괴형 철근콘크리트 보에서 순환골재의 치환율에 따른 음향방출 특성

김윤수 ( Kim Yun-soo ),이영오 ( Lee Young-oh ),전수만 ( Jeon Su-man ),윤현도 ( Yun Hyun-do ),유영찬 ( You Young-chan ) 한국구조물진단유지관리공학회 2006 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.10 No.2

Recently, construction wastes are increasing at every year. So problem of environmental pollution and lack of natural aggregates are serious. To solve this problem, the use of recycled aggregate is necessary. In this paper, failure behavior of recycled coarse aggregates reinforced concrete beam using recycled coarse aggregates with different replacement levels of recycled coarse aggregates are investigated using acoustic emission(AE). For these purpose, four recycled coarse aggregates replacement level (i.e 0%, 30%, 60% and 100%) were considered: As the load increased, AE signals were generated by internal crack propagation within the specimen. These AE signals were detected with AE sensors attached on the front of surface of the specimen. Result from this study show AE signal, AE method can apply to investigate a failure mode of reinforce concrete beam according to recycled coarse aggregates replacement level.

골재종류에 따른 콘크리트의 압축파괴 및 음향방출특성

김윤수 ( Kim Yun-soo ),김선우 ( Kim Sun-woo ),윤현도 ( Yun Hyun-do ),서수연 ( Seo Soo-yeon ),최창식 ( Choi Chang-sik ),최기봉 ( Choi Ki-bong ) 한국구조물진단유지관리공학회 2006 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.10 No.1

When concrete is subjected to uniaxial compression, the failure process is normally initialed from a localized zone. The localization of failure governs structural behaviors of concrete. Therefore, it is necessary to investigate the extent of the localized failure zone in compression. In this study, acoustic emission(AE) method is applied to distinguish the damage processes of concrete cylinder in compression, because the method can be applied to quantify the damage in concrete. Acoustic emission characteristics of concrete were investigated during the entire loading period. For these purpose, three kinds of concrete were used: NN series[Natural coarse aggregate and fine aggregate], NR series[Natural coarse aggregate and natural fine aggregate (50%) and recycled fine aggregate(50% substitute)] RN series[Recycled coarse aggregate and natural fine aggregate]. The influences of different aggregate in concrete on AE characteristics were investigated as well.

도시철도 최적 운영계획 수립에 관한 연구

김윤수(Kim Yun-Soo),박정수(Park Jung-Soo) 한국철도학회 2009 한국철도학회 학술발표대회논문집 Vol.2009 No.11월

In Seoul, Urban Railroad companies(total 9 line) have observed the position as the urban traffic system which solve the problem such as traffic gam and environment pollution in a response to traffic generation of ten million of the citizens of Seoul from opening of line 1 to line 9. Based on the safe operation, the operation planning of urban railroad is very important enough to consider economic efficiency, rationality, efficiency and user side. This study intend to be possible for good service as well as efficient operation by inquiring into establishment of The Best Operation Planing which transportation-demander comfortably and safely make use in Urban Railroad. Contrary to a leading study focused on only train diagram, train competition, this study aim at inquire into substantially necessary and overall factor on the basis of the train operation plan.