해수 저염분 순치과정에서 먹이섭취가 흰다리새우, Litopenaeus vannamei 유생에 미치는 영향

김수경,심나영,조지현,김종현,김수경 한국환경생물학회 2018 환경생물 : 환경생물학회지 Vol.36 No.3

저염분 순치기간에 먹이를 섭취함으로서 얻을 수 있는 초기 유생의 생리적 변화는 다음과 같이 요약할 수 있다. 하루 동안의 순치기간에 염분농도에 따른 유생의 생존율은 차이를 보였지만 각 염분구간별 먹이섭취 유무에 따른 생존효과는 유사하였다. 그러나 먹이섭취 시 유생이 에너지원으로 활용할 수 있는 cholesterol, triglyceride의 증가가 있었으며 삼투압에 영향을 미칠 수 있는 glucose 농도가 유의적으로 증가하였다. 또한 저염분 순치기간 동안에 스트레스, 조직손상 및 대사작용의 지표가 되는 BUN과 creatine의 감소가 있었다. 먹이섭취로 인한 소화효소의 활성이 먹이를 섭취한 실험구 모두에서 증가하였다. 그러므로 유생발달과 함께 중요한 소화, 순환, 생식기관이 형성되며 탈피 성장을 위한 신진대사가 빠르게 진행되는 유생시기에 저염분 순치가 효율적으로 이루어져 향후 지속적인 성장을 유도하기 위해서는 먹이를 섭취하는 것이 더 효과적인 것으로 판단되었다. This study focused on the effects of feeding on postlarvae of shrimp, Litopenaeus vannamei, during the identified acclimation time to low salinity. A total of 5 different salinity groups with or without feeding (32, 24, 16, 8, and 2 psu, 1 liter, triplicates) were prepared, and 30 shrimp were settled at PL21 (postlarvae) and placed in each group. After 24 hours of the experimentation process, the survival rate of the fed and starved groups was observed to be lower in the 2 psu group compared to other salinity groups, with the rate of 86.6% and 81.1%, respectively. The condition index of glucose and triglyceride, which are important factors for osmoregulation and as energy sources, was 4.2-7.6 times and 2.7-3.4 times higher in the fed groups than the starved groups at all the levels of salinities. The creatine level increased by 1.1-1.5 times in the starved groups as compared to the fed groups. Likewise, the activity of all the digestive enzymes like, lipase, α-amylase, trypsin, and alkaline protease were clearly higher in the fed groups (ANOVA, p<0.05). Apparently, it was observed that feeding is effective for the postlarvae of shrimp, which shows a characteristic fast metabolism and larval development, during the acclimation period to low salinity.

단백질원에 따른 흰다리새우 (Litopenaeus vannamei)의 성장효과

김수경,김은지,서형철,장인권,김수경 한국수산과학회 양식분과 2012 한국수산과학회 양식분과 학술대회 Vol.2012 No.6

바이오플락 탈질수가 어린 흰다리새우, Litopenaeus vannamei의 생존율 및 생리특성에 미치는 영향

김수경,장진우,조영록,김준환,김수경 한국환경생물학회 2019 환경생물 : 환경생물학회지 Vol.37 No.2

This study investigates the effect of denitrified biofloc water on changes in the water quality parameters and the physiological characteristics of shrimps. Biofloc rearing water contains a large number of microorganisms and can rapidly stabilize the water quality and energy saving if reusable due to high water temperatures. Rearing water contain floating bacteria with both aerobic and anaerobic bacteria. Therefore, when the carbon source is added in limited air supply, the anaerobic state is activated and the denitrification process is possible. In this study, the denitrification water had the following properties: ammonia (6.9 mg L-1), nitrite (0.3 mg L-1), nitrate concentration (9.2 mg L-1), high pH (8.42) and alkalinity (590 mg L-1). The experimental group consisted of seawater (SW, control), a mixture of Seawater and denitrified biofloc water (DNW) in the ratio of 3:1, 1:1 and DNW only. All experiments were done in triplicate. As a result, the survival rate never changed even when 100% of the denitrification water was utilized. However, a body fluid analysis showed that creatine and BUN were increased due to index of stress and the tissue damage resulting from the high denitrified water content. Body fluid ions (Na+, K+, and Cl-) significantly decreased as the denitrified water content increased. It was recommended that the denitrification water be mixed with a certain ratio (less than 50%) in the future as it may affect the osmotic pressure control in shrimps. 안정된 바이오플락 사육수에는 대량의 미생물들이 존재하고 있으며 사육수온이 높아 재사용이 가능할 경우 빠른 수질안정화 및 에너지 절약을 할 수 있다. 바이오플락 사육수 내 부유하고 있는 자가 및 타가 영양세균은 호기성과 혐기성 세균을 모두 포함하고 있어 탄소원을 넣고 산소를 공급하지 않는 혐기성 상태로 만들면 탈질과정이 가능 하다. 본 연구에서 바이오플락 탈질수의 특성은 암모니아(6.9 mg L-1), 아질산 (0.3 mg L-1), 질산농도 (9.2 mg L-1), 높은 pH (8.42), alkalinity (590 mg L-1)였으며 이 탈질수를 첨가한 사육수의 물리적 환경 변화가 어린새우의 생존 및 생리적 특성에 미치는 영향을 조사하였다. 그 결과 탈질수를 100% 사용하여도 생존율의 변화를 보이지 않았으나 혈림프를 포함한 체액 분석결과 탈질수 혼합에 의한 조직손상 및 스트레스 지표인 크레아틴, 혈중 요소성 질소의 증가가 관찰되었고 탈질수 혼합비율이 높을수록 새우 체내 이온(Na+, K+, Cl-)의 농도가 유의적으로 감소하여 향후 삼투압조절에 영향을 미칠 수 있는 것으로 나타남에 따라 탈질수를 일정비율로 (50% 미만) 혼합하여 사용하는 것이 바람직한 것으로 사료된다.

주부 김수경

김수경 충북연구원 2009 앞,뒤 바라보다 Vol.- No.2

흰다리새우 유생의 저염분 순치방법에 따 른 생화학적 특성변화

김수경,심나영,장진우,전제천,김수경,신윤경 한국환경생물학회 2017 환경생물 : 환경생물학회지 Vol.35 No.1

This study focused on the physiological change of the shrimp, Litopenaeus vannamei postlarvae 15 stages, under different acclimation methods up to the endpoint of 4 practical salinity unit (psu). Besides using sea water as the control, two acclimation methods, fast acclimation (50% salinity reduction every 8 hours) and slow acclimation (50% salinity reduction every day), were adapted. Results show that the survival rate, glucose and blood uric nitrogen of each group were not significantly different. However, the ion profile differed according to the acclimation methods. Magnesium and sodium of shrimps acclimated to low salinity in both the methods, showed lower concentration than shrimps at 32 psu sea water. Especially, Na+ concentration, which directly influences the osmolality of shrimp, decreased sharply in the fast acclimated group during the first eight hours (from 32 psu to 16 psu). To reduce acclimation stress, it is recommended to take more than eight hours during the first step for reducing the salinity.

전신성 경화증 환자에서 발생한 경구 Sildenafil (Phosphodiesterase-5 억제제) 투여로 호전된 수지궤양 1예

김수경,정현영,김지영,김성규,최정윤,박성훈 대한류마티스학회 2007 대한류마티스학회지 Vol.14 No.4

Systemic sclerosis is a connective tissue disease characterized by cutaneous and visceral fibrosis, as well as vascular disease involving arterioles, small and medium arteries of the peripheral circulation. Digital ulcers, defined as necrotic lesions that occur either at distal aspects of digits or over bony prominence, occur in up to 50% of patients with limited or diffuse systemic sclerosis. These lesions are exquisitely painful, heal slowly, and lead to substantial functional disability. We describe a 59-year-old woman with systemic sclerosis, suffering from a painful, non-healing digital ulcer despite conventional therapies, who demonstrated dramatic improvement with oral sildenafil treatment.

Synthesis and Charaterization Of Guanidylated Chitosan

김수경,Kaushik Singha,김원종 한국고분자학회 2008 한국고분자학회 학술대회 연구논문 초록집 Vol.33 No.2

臟器內 Pyruvic Acid 및 α-Ketoglutaric Acid 含量變動에 關한 硏究

金洙慶 우석대학교 의과대학 1967 우석의대잡지 Vol.4 No.2

The variations of both pyruvic acid and α-ketoglutaric acid value in the animal organs, viz. liver, brain, small intestines and gluteus muscles were measured for observing some metabolic changes of the animals during eight days fasting and refeeding after a period of four days starvation. The animals used were adult male albino rats from a pure strain, weighing 250-350g. The results obtained are summerized as follows; 1. The α-ketoglutaric acid contents in the organs decreased distinctly during early stage of starvation (small intestines and muscles were on the first day, and liver and brain were in the 2nd day of fast) compared with the control group. 2. The α-ketoglutaric acid value in the organs much more changed rather than that of pyruvic acid during 8 days starvation. 3. The pyruvic acid and α-ketoglutaric acids contents in the organs didn't show a signifcant change during 4 to 8 days starvation. 4. When refeeding after 4 days starvation, the α-ketoglutaric acid in the muscle significantly increased for the first 24 hours whereas the pyruvic acid and α-ketoglutaric acid in the liver, small intestines and brain once considerably decreased, thereafter they increased steadly. 5. When refeeding after 4 days starvation, the puruvic acid and α-ketoglutaric acid contents in the muscle was significantly changed than the other organs. 6. There was no significant difference on the keto acids values among the groups refed different quality of the diets for the period of 48 hours after four days fast. Therefore, as the above results showed. the most significant metabolic changes in the body does occur in the very early stage of fasting and refeeding.

Toll-like Receptors and Antimicrobial Peptides Expressions of Psoriasis: Correlation with Serum Vitamin D Level

김수경,박선,이은소 대한의학회 2010 Journal of Korean medical science Vol.25 No.10

To evaluate the association of Toll-like receptors (TLRs), antimicrobial peptides (AMPs) and vitamin D receptors (VDRs) in psoriasis, lesional (PP) and perilesional skin (PN) from psoriasis,atopic dermatitis (AD) patients and healthy controls (NN) were studied by immunohistochemistry. Compared with PN, AD and NN skin, dysregulated expression of TLRs, AMPs and VDR was detected in PP skin. Noteworthy, our results showed altered correlation between TLR2 and VDR expression in PP and PN skin. Human beta defensin 2(HBD2) and cathelicidin (LL-37) expressions in the PP skin were higher in serum vitamin D sufficient (VDS) groups than serum vitamin D deficient (VDD) groups. Negative correlation was found between TLR2 and VDR expression in the PP skin of VDD groups. However,positive correlation was noted in the PP skin of VDS groups. Based on the present results,therapies targeting the activity of TLRs, AMPs and vitamin D, including modulation of the TLR-VDR pathways, might provide new therapeutic approaches to the psoriasis and other inflammatory skin diseases.

미혼남녀의 자기관이 결혼가치관에 미치는 영향: 이성관계의 경험의 매개효과

김수경 한국여성심리학회 2019 한국심리학회지 여성 Vol.24 No.4

This study has investigated the mediating effects of heterosexual dating experiences on the relations between self-construals and marital values of 266 single males and females (134 males and 132 females) in their 20s and early 30s. As a result, first, significant differences were observed in self-construals, marital value, and dating experiences according to demographic variables such as gender, education, and economic level. Second, as a result of examining the main factors for predicting marital intention, the heterosexual dating experience, educational level, and marital values predicts marital intention significantly. The marriage intention was high. Third, as a result of conducting path analysis to verify the mediating effect of dating experience in the relationship between self-construals and marriage value of single males and females, interdependent self-construals influenced marital values through the experience of heterosexual relationship. Independent self-construals, on the other hand, had a direct effect on conservative marrital values. The results of this study showed that marital values differed according to the types of self-construals, and interdependent self-construals predicted the formation of a positively positive marital-values through a healthy dating relationship experiences.