급성 췌장염의 시대적 변화

김배영(Bai Young Kim),김효종(Hyo Jong Kim),정형근(Hyung Keun Chung),김영관(Young Kwan Kim),동석호(Seok Ho Dong),김병호(Byung Ho Kim),이정일(Jung Il Lee),장영운(Young Woon Chang),장린(Rin Chang) 대한내과학회 1993 대한내과학회지 Vol.45 No.5

Background: According to the rapid changes in social environment such as increasing alcohol consumption and the marked improvement in diagnostic technique, we expect that there have been major changes in various aspects of acute pancreatitis during the recent 12 years in Korea. Methods: The medical records of 317 patients with acute pancreatitis diagnosed from 1980 through 1992 were reviewed about clinical, laboratory and radiological findings in the three different time period. Results: 1) There has been an increasing incidence in male sex about three folds. 2) There has been an increasing trends in the duration of hospitalization. 3) A significant increase was observed in the proportion of patients with alcoholic pancreatitis in the three different time period; 26.9%, 45.7%, 50.7% respectively. 4) The complication rates have been slightly increased in the three different time period; 46.3%, 47.1%, 59.1% respectively. But, the type of complications has not been changed. 5) There is increasing severity in non-gallstone group, but no significant differences in gallstone group. There is a declining trends in the number of patients with muld severity index, but increasing trend in the number of patients with moderate severity index in non-gallstone group. 6) There was no significant differences in mortality rate. Conclusions: There has been major changes in the etiology ans severity of acute pancreatitis from 1980 through 1992. Especially, significant increase in the proportion of patients with alcoholic pancreatitis was observed in the three differnt time period. These results might be due to socioeconomic changes.

인삼이 흰쥐의 일반활동에 미치는 영향

김병호,서정민,장임수,Kim, Byung-Ho,Suh, Chung-Min,Chang, Im-Soo 대한생리학회 1973 대한생리학회지 Vol.7 No.2

A study was planned to see if the Panax Ginseng has any influence upon the general activity of rats. Seventeen male rats (mean body weight: 204 g) was divided into a ginseng group (N=9) and a saline group (N=8). Once a day for 5 days they received subscutaneously 0. 5m1/100g body weight of ginseng extract solution (4 mg of ginseng alcohol extract in 1 ml of saline), and same amount of saline, respectively. For 20 days beginning from the 1st day of medication on, each rat received activity measurement by means of beam interruption method. Following results were obtained : 1. Activities at night (6 p.m. to 6 a.m. next morning) and in 24hours on the 10 th, 12th, and 13 th day were significantly increased in the ginseng group compared with activities of the saline group. 2. Summed total night and 24-hour activities observed for 15 days after termination of medication were also significantly augmented in the ginseng group compaired with those of the saline group, although no remarkable difference existed between the 2 groups with regard to the summed total daytime activity. It is inferred that the ginseng augments general activity of rats especically at night.

신라고비의 모형제작

김병호,이용희,김창석,Kim, Byeong-Ho,Lee, Yong-Hui,Kim, Chang-Seok 국립문화재연구소 1989 保存科學硏究 Vol.10 No.-

담관암세포주에서 내인성 peroxisome proliferator-activated receptor gamma 배위자인 15-deoxy-delta(12,14)-prostaglandin J2의 항암 효과

정성훈 ( Sung Hoon Jung ),김병호 ( Byung Ho Kim ),김영일 ( Young Il Kim ),심재준 ( Jae Jun Shim ),황보영 ( Bo Young Hwang ),장재영 ( Jae Young Jang ),동석호 ( Seok Ho Dong ),김효종 ( Hyo Jong Kim ),장영운 ( Young Woon Chang ),장 대한내과학회 2010 대한내과학회지 Vol.78 No.1

Background/Aims: Peroxisome proliferator-activated receptor (PPAR)-γ ligand is known to inhibit the growth of several kinds of cancer cells, yet its effect on cholangiocarcinoma is indecisive. We investigated the effect of an endogenous ligand of PPAR-γ, 15-deoxy-δ (12,14)-prostaglandin J2 (15-deoxy-PGJ2) on cholangiocarcinoma cells that were established from intrahepatic cholangiocarcinoma tissue of Korean patients. Methods: Four cholangiocarcinoma cell lines, Cho-CK, Choi-CK, JCK and SCK, were studied. The mRNA expression of PPAR-γ, bcl-2, and bax were examined by RT-PCR. Cell viability was determined by MTT assay. The cell cycle was analyzed by flow cytometry, and apoptosis by cell death detection ELISA kit. Caspase activity was measured by colorimetric assay. The effect of caspase inhibitors on 15-deoxy-PGJ2-induced apoptosis was determined by measuring cell viability using the MTT assay. Results: PPAR-γ mRNA was expressed in all cholangiocarcinoma cells. 15-deoxy-PGJ2 inhibited proliferation of all cells in a dose- and time-dependent manner. All cells treated with 15-deoxy-PGJ2 showed increased dose-dependent apoptosis. Caspase 3 was activated in all cells and caspase 9 was activated in all but JCK cells after 15-deoxy-PGJ2 treatment. Caspase 8 activity showed no significant change. The pan-caspase inhibitor, Z-VAD-FMK, and the caspase-3 inhibitor, Z-DEVD-FMK, blocked 15-deoxy-PGJ2-induced apoptosis in all cells dose-dependently. The expression of bcl-2 was decreased in Cho-CK, Choi-CK and SCK cells, and bax expression was not changed significantly after 15-deoxy-PGJ2 treatment. Conclusions: PPAR-γ mRNA was expressed in all Korean cholangiocarcinoma cells. Our data suggest that 15-deoxy-PGJ2 exerts an antineoplastic effect against cholangiocarcinoma cells by inducing apoptosis through caspase activation. (Korean J Med 78:75-86, 2010)

속박 (束縛) 과 침수 (浸水) 로 유도된 흰쥐의 스트레스성 급성 (急性) 위점막병변 (胃粘膜病變) 발생에 있어서 위산분비의 (胃酸分泌) 역할

민영일(Young Il Min),박충기(Choong Kee Park),장린(Rin Chang),김병호(Byung Ho Kim),장영운(Young Woon Chang),이정일(Jung Il Lee),박승준(Seung Joon Park),정주호(Joo Ho chung),정지창(Jee Chang Jung) 대한소화기학회 1989 대한소화기학회지 Vol.21 No.1

N/A The possible roles of gastric acid secretion in the pathogenesis of stress ulcers induced by restraint with water-immersion for 7 hours in pylorus-ligated rats were investigated. Control rats with pylorus-ligation only did not show any gastric mucosa} lesion, and their mean gastric acid output was 449.2 ± 36.1 umol/rat. In contrast, the stressed rats, group II, showed multiple, punctate and hemorrhagic erosions in gastric mucosa, and mean gastric acid output was decreased to the level of 253.7 ± 27.3 umol/rat. Subcutaneous administration of histamine(40 mg/kg every 2.5 h for a total three times) to stressed rats augmented the lesion with severe multiple, linear and hemorrhagic erosions and mean acid output increased significantly (328.1 ± 31.5 umol/, p<0.005) compared with group II. Pretreatment with rantidine(20 mg/kg) or prostaglandin E2 (30 ug/kg) before exposure to stress reduced gastric lesion formation slightly with no significance. In ranitidine pretreated rats, mean gastric acid output was decreased significantly (120.1 ± 15.3 umol/rat, p<0.005) compared with group II. However, in prostaglandin pretreated rats, mean gastric acid output showed no significant changes. Therefore, it is suggested that increased gastric acid secretion seems to be an aggravating or a permissive factor rather than a major factor in the pathogenesis of stress-induced gastric mucosal lesions.

SV40 T 항원의 온도조건부 변이형 유전자가 포함된 Amphotropic Retrovirus 에 의한 사람 태아 간세포의 불멸화

이정일(Joung Il Lee),동석호(Seok Ho Dong),김효종(Hyo Jong Kim),김병호(Byung Ho Kim),장영운(Young Woon Chang),장린(Rin Chang),성세라(Se Ra Seong),박재경(Jae Kyung Park),김승보(Seung Bo Kim),이상목(Sang Mok Lee) 대한내과학회 1999 대한내과학회지 Vol.57 No.1

Human cells are almost never spontaneously immortalized in vitro. We tried to immortalize human fetal hepatocytes (h-FH) and evaluate the differentiational status and its change. Methods : Hepatocytes were isolated from a liver fragment of 20 week old fetus and infected with amphotropic recombinant retrovirus containing a temperature- sensitive mutant of SV40 large T antigen and neomycin phosphotransferase gene. G418 resistant colonies were cloned and expanded. The cells which were able to divide more than 30 times were used to analyze various functions. Results : The immortalization rate was 3.3 x 10-8 and two cell lines (C11, D21) were established. C11-60, C11-80, D21-30 and D21-60 (suffix number means the cell division counts) were evaluated. D21-30 was thougt to be imcompletely immortalized because a considerable portion of cells died during culture. The morphology was similar to that of epithelial cells except for D21-30 which looked like fibroblast. The cells grew rapidly at 33oC but stopped growing at 39oC. T antigen and p53 was expressed at 33oC but disappeared at 39oC, which suggest that T antigen binds to p53. Chromosomal changes were so marked that it was impossible to discriminate exact number. Albumin was secreted as about 1/10 as that of h-FH, but alpha-fetoprotein secretion stopped after immortalization. Telomerase was activated in both cell lines except for the incompletely immortalized cells D21-30. Telomere was elongated in competely immortalized cell lines, but it was rather shortened in D21-30 compared to that of h-FH. Macroscopic colonies did not develop in soft agar assay. Conclusions : We successfully immortalized human fetal hepatocytes. Although the cells are not likely to have oncogenicity, the functions are not so good, possibly due to marked chromosomal changes which are thought to occur before telomerase is activated during immortalization step.

Galactosamine으로 급성간부전이 유도된 흰쥐에서 간세포이식의 효과

김병호(Byung Ho Kim),은연기,김효종(Hyo Jong Kim),장영운(Young Woon Chang),장린(Rin Chang),이정일(Jung Il Lee) 대한내과학회 1991 대한내과학회지 Vol.40 No.4

Although the mortality rate following acute hepatic failure is still high, it is possible to recover completely if patients survive. So temporary hepatic support that can sustain life during the period of hepatic insufficiency is important in the treatment of acute hepatic failure. Despite the fact that various therapeutic modalities have been described for treating acute liver failure, none has proven to be clinically useful except for the liver transplantation. But liver transplantation is not used widely because of the difficulty in obtaining cadaver donor organs especially in eastern countries, techinical difficulties, and high cost. As the alternatives to liver transplantaion, several investigators developed various techniques of hepatocellular transplantation, which has significantly enhanced the survival rate in the animal from chemically or surgically-induced acute liver failure. According to the evidence provided by the aforementioned studies, we investigated the efficacy of allogeneic hepatocellular transplantation on the survival rate following galactosamine-induced acute liver failure in the Sprague-Dawley male rat, The results were as follows: 1) In the preliminary study for determining the amount of d-galactosamine HC1 (Sigma), intraper- itoneal injection of 1.3g/kg of galactosamine induced a mortality rate of 75% within 5 days in 12 rats (210~280g). 2) Isolation of hepatocytes was performed by a modification of the method of Seglen, collagenase (Sigma, type I) two-step perfusion technique. The mean number of obtainable total hepatocytes from 7 rats was 2.2 ×108±0.7×108, with an average viability of 50±3%. 3) There was no significant difference in the survival rate between group I, which was administered only galactosamine, and group II, which was treated by intraperitoneal transplantation of 2×107 viable hepatocytes at 51 to 55 hours after administration of galactosamine (33% VS 40%). In conclusion, we couldn't note any improvement of survival after hepatocellular transplantation in rats with galactosamine-induced acute hepatic failure. But this result doesn't mean that hepatocellular transplantation had no effect in the treatment of acute hepatic failure. In the future, we will need further study about our method of hepatocytes isolation to increase the viability of isolated hepatocytes, adequate time of the transplantation after galactosamine administration, and syngeneic hepatocellular transplantation in order to evaluate more exactly the efficacy of hepatocellular transplantation.

내시경적 정맥류 결찰요법의 단기 치료성적 및 안정성 - 내시경적 경화요법과의 비교 -

김병호 ( Byung Ho Kim ),김효종 ( Hyo Jong Kim ),김영관 ( Young Kwan Kim ),장린 ( Rin Chang ),이정일 ( Joung Il Lee ),동석호 ( Seok Ho Dong ),장영운 ( Young Woon Chang ) 대한소화기학회 1994 대한소화기학회지 Vol.26 No.5

N/A Endoscopic sclerotherapy is an effective method for bleeding esophageal varices, however it is often associated with local and systemic complications which may limit its usefulness. A newly developed endoscopic treatment for bleeding esophageal varices, endoscopic variceal li- gation, which uses small rubber bands to occlude and eradicate the varices, may cause less damage to the esophagus and have no systemic complication theoretically. We compared the effectiveness and safty of the two techniques retrospectively. The patients had proved bleeding from esophageal varix and showed advanced liver cirrhosis. Nineteen patients were treated with ligation, and 20 patients with sclerotherpy. They were followed for a mean of 4 months. Acitve bleeding was controlled by ligation in 2 of 3 patients(67% ) and by sclerotherapy in 3 of 4 patients(75%). The eradication of varices was achieved by ligation in 6 of 14 patients(43 A ) and by sclerotherapy in 10 of 15 patients(67% ). But the mean number of treatments with ligation was lower than that with sclerotherapy(3.4 vs 4.2) ar J than other ligation treatment group(4-5). There was no significant difference in recurrent hernorrhage between two groups. There were one case of pleural effusion and one case of sepsis with acute renal failure in scle- rotherapy treated-patients, whereas none of these complications occurred in ligation treated patients. Two cases of treatment induced bleeding were developed in ligation group. Endoscop- ic variceal ligation has fewer local and/or systemic complications than sclerotherapy. To eradi- cate varices more completely and to achieve denudation of lower esophageal mucosa for pre- vention of recurrent hemorrhage, combination with sclerotherapy would be effective rather than ligation alone.(Korean J Gastroenterol 1994; 26: 765 770)

Alendronate 복용으로 유발된 위궤양 1 예

김병호(Byung Ho Kim),정용희(Young Hee Joung),장영운(Young Woon Chang),민선기(Sun Kee Min),문주영(Ju Young Moon),한요셉(Yo Seb Han),이동근(Dong Keun Lee),동석호(Seok Ho Dong),김효종(Hyo Jong Kim),이정일(Jung Il Lee),장린(Rin Chang) 대한소화기학회 2001 대한소화기학회지 Vol.38 No.4

Alendronate (Fosamax), an aminobisphosphonate and a selective inhibitor of osteoclast-mediated bone resorption, is used to treat postmenopausal osteoporosis and Paget's disease. In recent years, reports of severe esophageal injury in patients who took alendronate have evoked attention to the irritant effects of this drug on the mucosa of the upper gastrointestinal tract. We present a case of gastric ulcer caused by alendronate. The patient chiefly complainted of epigastric pain during 14 days. She had taken mefenamic acid for pain control for 3 years and she took once-weekly 70 mg of alendronate for 2 weeks for treatment of osteoporosis before the symptom started. Upper gastrointestinal endoscopy revealed two active gastric ulcers in the low body. The results of rapid urease test (CLO test) and modified Giemsa test for the detection of Helicobacter pylori infection were negative. After withdrawal of alendronate, the symptom resolved completely in one week. Six weeks later, follow-up endoscopy showed complete healing of the ulcer. (Korean J Gastroenterol 2001;38:284-287)

간경변환자의 Na 및 수분저류에 있어서 혈중 Atrial Natriuretic Polypeptide 의 병인론적 역할

김병호(Byung Ho Kim),이정일(Joung Il Lee),장영운(Young Woon Chang),장린(Rin Chang),민영일(Young Il Min) 대한내과학회 1989 대한내과학회지 Vol.36 No.6

N/A The aim of this study was to investigate the possible role of ANP in sodium and water retention in patients with liver cirrhosis. The dynamic change of plasma ANP to acute water loading (intravenous loading of 5% dextrose solution, 20 ml/kg of body weight within 20-30 minutes) in 14 stable cirrhotic patients and 6 normal controls was measured. In addition, the change of urinary excretion of sodium after water loading was also observed. 1) Fourteen patients with liver cirrhosis were classified in 2 groups according to the rate of urinary excretion to water load, that is, patients excreting more than 85% of the water load over the next 160 minutes were defined as excretor (group II) and patients excreting less than 85% as nonexcretor (group III). Six normal controls were defined as group I. 2) The basal level of plasma ANP was significantly higher in cirrhotic patients (group II; 21±7.5pg/ml, group III; 27.2±13.5 pg/ml) than in normal control subjects (undetectable level). The plasma ANP level was also more increased in the cirrhotics of group II and group III(130.4±25.9 pg/ml, 154.2±27 pg/ml) at 40 minutes after water loading than in normal controls (47.6±6.1 pg/ml, group II; p<0.05, group III; p<p.025). 3) At 40 minutes after water loading, urinary excretion of sodium was increased significantly in all 3 groups (p<0.005, p<0.005, p<0.025). But the level of urinary sodium excretion of group III was markedly lower than that of normal controls (p<0.005), and patients in group II excreted a similar amount of sodium to normal controls. 4) The maximal ANP level correlated with the maximal urinary sodium excretion after water loading in normal controls (r = 0.7, p < 0.1), but there was a tendancy of a reverse correlation in group II (r = -.074, p<0.05). 5) Ten cirrhotic patients with ascites had no significant correlation between 1 plasma ANP and basal plasma AVP (r = 0.13), And there was no significant correlation between basal plasma ANP and basal plasma norepinephrine in 7 cirrhotic patients with ascites (r =-0.46). In conclusion, increased ANP in cirrhatics might have occurred as a result of decreased response to ANP in the kidney, and this decreased response to ANP might cause sodium and water retention in patients with liver cirrhosis. In the future, more detailed study about the ANP receptor might be required to clarify the cause of decreased sensitivity to ANP in the kidney.