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지방간에 대한 혈청 r-glutamyl transferase ( GGT ) 의 진단적 유용성 및 다른 인자와의 상관관계
최권(Kwon Choi),김병익(Byung Ik Kim),조용균(Yong Kyun Cho),박창영(Chang Young Park),손정일(Jung Il Sohn),전우규(Woo Kyu Jeon),김향(Hyang Kim),정을순(Eul Soon Chung),금동극(Dong Geuk Keum),이화영(Hwa Young Lee),이상종(Sang Jong Lee) 대한내과학회 1999 대한내과학회지 Vol.57 No.6
N/A Background : Gamma-glutamyl transferase(GGT) has found wide application as a diagnostic test in hepatobiliary disease, and has been used as the best single marker of alcohol intake. In spite of the wide use of GGT in clinical practice, knowledge concerning the distribution and the determinants of this risk factor in the normal population is spared in Korea. We tried to obtain a better evaluation of specificity of serum GGT by analysis of a large population of health examination. Methods : GGT was measured in 17,140 males aged 17-86 years and 12,125 females aged 18-90 years screened in a health survey program. Results : In multiple regression analyses, serum GGT level showed strong positive association with fatty liver, body mass index, serum levels of AST, ALT triglyceride, uric acid, alkaline phosphatase, systolic blood pressure, and diastolic blood pressure, and weakly positive association with serum levels of creatinine, total cholesterol, and fasting blood sugar. In females, menopause were positively associated with GGT. Conclusions : An elevated serum GGT levels is a strong indicator of hepatobiliary dysfunction or fatty liver. However, proper interpretation of a serum GGT elevation should be carefully considered in correlation with clinical data and laboratory findings. (Korean J Med 57:1006-1013, 1999)
금동극,김병익 대한알레르기학회 1999 천식 및 알레르기 Vol.19 No.6
Background and objective : The selection of allergen panels is a prerequisite to effectively test for innumerable allergens scattered throughout the environment. However, the selection of the pre-existing panel has been vague and contains some allergens that have not been verified as being common in Korea. This study was aimed to produce allergen panels in Korea. Methods : For 12 months in 1996, sera were tested by the chemiluminescent assay of Multiple allergen simultaneous test (MAST-CLA: Immunosystems, Mountain view, U.S.A.). A total of 2, 467 specimens that either tested positive or were negative but had high total IgE level were pooled together. The pooled ser a were assayed for 60 allergens supplied by Dexall Acti Tip System (Dexall biomedical Labs. Inc., Gaithersburg, U.S.A.), a recently developed enzyme immu- noassay. According to the Allerg Ens Unit (Allergen Unit:AU), 12 of the most frequently encountered and 6 of the leaot frequent allergens with reactions between classes 3 and trace were selected. Results : The 12 most frequently encountered allergens were : Dermatophagoides pteronyssinus, Dermatophagoides farinae, house dust, timothy grass, perennial rye, mugwort, birch, oak, hazel nut, common ragweed, alder and dog dander. The 6 least freque- ntly encountered were : wheat, egg-white, cat epithelium, milk, cockroach and shrimp. Conclusion : The 12 allergens we chose proposed to be the minimally required panel of frequently encountered allergens in allergy testing. We conclude that the 12 most frequent allergens should be tested with the total IgE level as a major panel (panel-M) and that the 6 least frequently encountered allergens may be tested separately when needed, as a minor panel (panel-m).
Dot-Blot Hybridization을 利用한 Chlamydia trachomatis의 診斷
금동극,최태열,조삼현 대한감염학회 1995 감염 Vol.27 No.2
배경: C.trachomatis의 진단은 군체 배양의 어려움이 많이 있어 최근 gene probe 및 PCR등의 분자 생물학적 방법이 널리 사용되고 있다. 이에 저자들도 C.trachomatis의 MOMP를 암호 하는 gene probe "pFEN 50"을 진단에 사용하여 세포배양 결과와 비교 분석하였다. 방법: pFEN 50를 gene probe로 사용하여 표준균주 및 자궁내막염 환자 80예의 자궁경부 검체에서 C. trachomatis를 검출하기 위하여 세포배양과, 더불어 dot-blot hybridization을 실시하였다. 결과: Gene probe pFEN 50는 Bam HI으로 처리한 C.trachomatis DNA의 9.8kb 편절과 강하게 반응하였다. pFEN 50는 14종의 C.trachomatis와 반응하여 모두 양성반응을 나타내었으나 C.pneumoniae의 원인균인 TWAR 뿐만아니라 S.aureus, Coagulase negative staphylococcus, Gram positive bacilli, α-hemolytic streptococcus, β-hemolytic streptocococus, E.cloacae, A.lowffi, P. aeruginosa, E.coli, K.pneumoniae와는 일체 반응하지 않는 종특이 gene probe였다. pFEN 50의 예민도는 LGV typeⅡ의 DNA를 희석하여 실험한 결과 100pg까지 측정 가능하였다. 임상검체 80예에서 gene probe 및 세포배양에서 양성은 3명, gene probe 양성 배양음성은 3예, gene probe 음성 배양양성은 1명 그리고 모두 음성은 73명 이었다. 결론: 상기 결과로 보아 pFEN 50 gene probe를 이용한 ,Dot-blot hybridization은 C.trachomatis 진단에 유용하게 나타났다. Background: Chlamydia trachomatis is an important cause of sexually transmitted disease. Diagnostic tests based on the recognition of DNA sequences have been developed. The DNA probe assay has been described to detect specific parts of chlamydial nucleic acids. We evaluated the gene probe( pFEN 50) encoding the MOMP of C.trachomatis for detection of trachomatis from cervical specimens of 80 patients with pelvic inflammatory disease. Methods: Chlamydial culture was performed on cyclohexamide(2㎍/ml)-treated McCoy cell monolayer in shell vials, and the specimens were spotted onto Nytran-plus after boiling monolayer in shell vials, and the specimens were spotted onto Nytran-plus after boiling in lysing buffer(50mM Tris-HCl, pH 7.5; 1% Triton X-100; 1mM EDTA, ProteinaseK 400㎍/ml). Hybridization was then carried out. Results: The nucleic acid(pFEN 50) was hybridized with one 9.8 kb fragment from Bam HI-cleaved C.trachomatis(L2) DNA. The sensitivity of the pREN50 was 100pg DNA. No binding of the pFEN 50 to C.pneumoniae(TWAR) and other microorganisms(Staphylococcus aureus, coagulase negative staphylococcus, Grams-positive bacilli, α-hemolytic streptococcus, β-hemolytic streptococcus, Enterobacter cloacae, Acinetobacter lwoffi, Pseudomonas aeruginosae, Escherichia coli, Klebsiella pneumoniae) was observed. Three were positive for chlamydial culture and dot-blot hybridization. Four of eighty cases showed inconsistent results. Three of the four cases were culture negative, gene probe positive, which might be explained as false negativity of culture. One case was culture positive and gene probe be explained as false negativity of culture. One case was culture positive and gene probe negative, which might be due to the presence of dot-blot hybridization inhibitors in sample or the absence of inclusion bodies. Conclusion: We conclude that the dot-blot hybridization assay can be used to demonstrate the presence of C. trachomatis in many clinical specimens simultaneously. In addition, the pFEN 50 nucleic acid is a highly sensitive and specific gene probe for the detection of all serovar of C.trachomatis in clinical specimens.
비임균성 요도염 환자에서 Chlamydiazyme을 이용한 Chlamydia trachomatis의 검출에 관한 연구
김춘원,최태열,정규봉,금동극,김기동 한양대학교 의과대학 1986 한양의대 학술지 Vol.6 No.1
Chlamydia trachomatis causes a wide variety of human disease and especially importance is noted as a causative organism of non-gonococcal urethritis (NGU) and/or postgonococcal urethritis (PGU) and we here report the result of detection of C. trachomatis among patients with NGU and PGU with brief review of literature. The Chlamydiazyme test (Abbott Laboratories) is a new enzyme immunoassay for detecting C. trachomatis antigen is specimens from the urethra in men and the endocervix in women. We performed a prospective evaluation of the Chlamydiazyme assay in a V.D. clinic of Choong-ku Public Health Center in Seoul. The results were following: 1. Total positive rate was 17.5% (10/57) 2. Positive rate in NGU was 17% (9/53). 3. Positive rate in PGU was 25% (1/4).