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권지웅 ( Ji Wung Kwon ),이희권 ( Hee Kwon Lee ),박희전 ( Hee Jeon Park ),송지영 ( Ji Young Song ) 한국식품조리과학회(구.한국조리과학회) 2012 한국식품조리과학회지 Vol.28 No.1
This study was carried out to evaluate biological activities concerning extracts according to extraction methods from unripened fruit of Rubus coreanus Miq. The extraction methods were HWE (hot water extraction for 4 hr at 100℃), SFE (extraction for 3 hr at 40℃ under 300 bar, 100% of CO2 fluid), USE (ultrasonification extraction for 4 hr at 50℃ with water), USE+HWE (hot water extraction for 2 hr at 100℃ after ultrasonification process for 2 hr), VE (vacuum extraction for 4 hr at 90℃ under 0.9 bar with water). VE extract showed the highest contents of total polyphenol (178.78±3.79 mg/g) and total flavonoid (40.93±0.68 mg/g). IC50 values of DPPH radical scavenging activity, linoleic acid peroxidation inhibition activity and LDL (low density lipoprotein) oxidation inhibition activity of HWE extract showed the lowest 35.39±0.25 μg/mL, 12.61±0.31 μg/mL and 1.31±0.02 μg/mL among other all extracts, respectively. IC50 values of α-glucosidase inhibitory activities of VE and HWE extracts showed lower 14.34±0.20 μg/mL and 15.83±0.20 μg/mL than those of other extracts, respectively. Specifically, HWE and VE extracts have relatively better biological activities than other extracts; these could be potentially used as a bioactive source for health functional foods.
Opuntia ficus-indica 다당 A-1의 특성 및 알코올유도 간 산화스트레스의 보호 효과
류일환,권지웅,이어진,윤용갑,권태오,Ryu, Il-Hwan,Kwon, Ji-Wung,Lee, Eoh-Jin,Yun, Young-Gab,Kwon, Tae-Oh 대한한의학방제학회 2009 大韓韓醫學方劑學會誌 Vol.17 No.2
Reactive oxygen species(ROS) can induce hepatotoxicity and trigger apoptosis in the liver. In this study, we investigated the sulfated polysaccharide A-1 from Opuntia ficus-indica against alcoholic oxidative stress in human liver Hep G2 cell. An antioxidant substance A-1 obtained from the enzymatic extract of Opuntia ficus-indica fruit was purified by DEAE-cellulose ion exchange and sephadex G-100 gel permeation chromatography. The purification yield and molecular weight were 14.3% and 1.8 KDa, respectively. The A-1 predominately contained arabinose, galactose, rhamnose and also sulfate group. The structure of A-1 was investigated by periodate oxidation, FT-IR spectroscopy, $^1H$-NMR spectroscopy. The A-1 mainly composed of alternating unit of ${\rightarrow}4$)-$\alpha$-L- Rapp-2-$SO_3^-$-$\alpha$-L-Galp-($1{\rightarrow}$ and branched linkage of $\beta$-D-Arbp- ($5{\rightarrow}$. The antioxidative activity was measured using the SOD, CAT activity and GSH assay, respectively. The expression of Nrf2 protein was analyzed by western blotting. The viable cell count analyzed by autofluorescence. Oxidative stress induced by ethanol(1 M) were dramatically reduced by A-1 treatment. A-1 also prevented cell death induced by oxidative stress. It also increased expression Nrf2 protein level. We concluded that sulfated polysaccharide A-1 from Opuntia ficus-indica effectively protect Hep G2 liver cell from alcoholic oxidative stress.
초음파 처리에 의한 검정라즈베리 부위별 에탄올 추출물의 산화방지 활성
김기안(Ki An Kim),권지웅(Ji Wung Kwon),김용석(Yong-Suk Kim),박필재(Pill Jae Park),채규서(Kyu Seo Chae) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.4
본 연구는 검정라즈베리 부산물인 줄기, 잎 및 씨 초음파 추출물의 산화방지 활성에 미치는 영향을 살펴보고, 산화방지 활성을 가지는 식품 및 의약품의 개발 가능성을 알아보기 위하여 수행되었다. 검정라즈베리 부위별을 물 및 25, 50, 75, 100% 에탄올을 추출용매로 초음파 추출하여 실험을 진행한 결과 총 폴리페놀 함량의 경우 검정라즈베리 줄기의 25, 50% 에탄올 추출물이 각각 348.21±5.40, 343.39±5.94 ㎎/g으로 가장 높은 함량을 보였고, 잎은 50% 에탄올 추출물이 161.06±3.57 ㎎/g, 씨의 경우는 75% 에탄올 추출물이 266.29±2.51 ㎎/g으로 나타나 줄기의 총 폴리페놀 함량이 가장 높음을 확인하였다. 페놀성 화합물 10종의 분석 결과 모든 시료에서 갈산과 엘라그산이 가장 높은 함량의 화합물로 분석되었고, 쿼르세틴, 페룰산, 쿠마르산, 카페산, 루틴, 루테올린은 모든 추출물에서 분석되었다. 총 플라보노이드 함량을 측정한 결과에서는 추출용매간에 함량에 차이가 나타나는 것을 확인하였는데, 줄기는 25% 에탄올 추출물이 76.02±4.23 ㎎/mL로 가장 높은 함량을 보였고, 잎의 경우는 50% 에탄올 추출물이 82.28±0.54 ㎎/mL로 가장 높았으며, 씨의 경우는 75% 에탄올 추출물이 82.40±2.49 ㎎/mL로 가장 높게 나타났다. 산화방지 활성 검증을 위한 DPPH 및 ABTS 라디칼 소거활성의 EC<SUB>50</SUB> 값을 비교한 결과 총 폴리페놀 함량이 가장 높았던 줄기의 활성이 가장 높았으며, 씨>잎 순으로 높은 라디칼 소거활성을 보였고, 추출용매 조건에 따라서 활성의 차이가 크게 나타났다. 또한 환원력을 측정한 결과에서도 줄기>씨>잎 순으로 높은 환원력을 보였고, 검정라즈베리 부산물인 줄기, 잎 및 씨 모두 높은 수준의 산화방지 활성을 가지는 것으로 확인되었으며, 추출조건으로는 줄기의 경우 25, 50% 에탄올, 잎의 경우 50% 에탄올, 씨의 경우 75% 에탄올 용매 조건에서 산화방지 활성이 가장 우수한 것으로 나타났다. 결과적으로 검정라즈베리 부산물인 줄기, 잎 및 씨 모두 산화방지 활성이 우수한 천연소재로서의 이용이 가능할 것으로 판단되었고, 특히 줄기의 경우 산화방지 활성이 매우 뛰어나 건강기능 식품 및 의약품과 같은 산업적 이용 가치가 높을 것으로 판단된다. This study was carried out to investigate the antioxidant effects of different parts (stems, leaves, and seeds) of the black raspberry for utilization as food materials. Different parts of the black raspberry were subjected to extraction via ultra-sonication extraction methods using water and ethanol at various concentrations (25, 50, 75, and 100%). Antioxidant capability of the extracts were determined by amounts of phenolic compounds, with flavonoid contents, radical scavenging activity, and reducing power. Irrespectively of ethanol concentration, extracts of stem showed the highest total phenolic compounds and antioxidant activities among different parts of black raspberry. The total phenolic compounds extracted from the black raspberry stem using 25 and 50% ethanol showed 348.21±5.40 and 343.39±5.94 ㎎/g, respectively. Fifty percent ethanol extracts of the black raspberry stem showed the highest DPPH (EC50 value: 60.89 ㎍/mL) and ABTS radical scavenging activities (EC50 value: 82.57 ㎍/mL). Further, 25% ethanol extacts of the black raspberry stem (0.263±0.004) was found to have the highest reducing power. The highest antioxidant activity of black raspberry stem indicates that black raspberry stem may be useful source for functional food.
최혜란(Hye Ran Choi),이수정(Su Jung Lee),이정현(Jung-Hyun Lee),권지웅(Ji Wung Kwon),이희권(Hee Kwon Lee),정종태(Jong Tae Jeong),이태범(Tae-Bum Lee) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.12
We investigated the effects of unripe black raspberry water extract (UBR-W) and oxidation-LDL treatment on cholesterol levels. Experiments using an established human hepatocellular carcinoma cell line (HepG2) showed a time-dependent increase in expression of LDL receptor after UBR-W treatment. Expression of LDL receptor-related genes, such as SREBP1 and 2, increased upon UBR-W treatment. However, expression of HDL-related genes was unaffected by UBR-W. HMG-CoA reductase activity was reduced by UBR-W treatment, whereas HMG-CoA mRNA expression significantly increased. In addition, the ApoB/ApoA1 mRNA level, which is a predictor of cardiovascular risk, was reduced in a time-dependent manner by UBR-W treatment. Macrophage-like cells (RAW 264.7) showed increased expression of ox-LDL-related genes, such as CD36, scavenger receptor-A, adipophilin, and PPAR-gamma, upon ox-LDL treatment compared to untreated control cells, and quantitative lipid analysis indicated a dramatic increase in lipid accumulation. However, UBR-W treatment significantly reduced expression of ox-LDL-related genes and largely prevented lipid accumulation. The results indicate that UBR-W mediates a cholesterol-lowering effect via inhibition of cholesterol synthesis and induction of LDL uptake through SREBP.
젖산발효에 의한 복분자, 아로니아, 오디, 블루베리의 이화학적 특성 및 항산화활성 변화
채규서(Kyu Seo Chae),류은혜(Eun-Hye Ryu),박희란(Hee Ran Park),권지웅(Ji Wung Kwon),박희전(Hee Jeon Park),송지영(Ji-Young Song) 한국식품조리과학회 2017 한국식품조리과학회지 Vol.33 No.5
Purpose: This study was carried out to investigate the changes in physicochemical properties and antioxidant activities of berries (black raspberry, black chokeberry, mulberry and blueberry) occurred during lactic acid fermentation. Methods: Berries were fermented at 30°C for 24 hours using Leuconostoc mesenteroides SRL6, after which total polyphenolic, total flavonoid, and total anthocyanin contents, DPPH and ABTS radical scavenging activities, and reducing power were measured. Results: Total acidities of lactic acid-fermented berries increased while pH values decreased. Fermented berries were measured for their total polyphenolic, total flavonoid, and total anthocyanin contents, DPPH and ABTS radical scavenging activities, and reducing power. Contents of total polyphenols and total anthocyanins of berries were slightly elevated after fermentation. Further, DPPH and ABTS radical scavenging activities were elevated after fermentation by Leu. mesenteroides SRL6. Reducing power of fermented berries was higher than that of non-fermented berries. Conculsion: These results suggest that black raspberry, black chokeberry and blueberry fermented by Leu. mesenteroides subsp. mesenteroides SRL6 have potential as functional materials in the food industry.