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근권 길항세균 Pseudomonas fluorescens KR164 에 Bacillus thuringiensis HD - 1 유전자의 삽입과 발현
김영일,이영환,강흔수 한국농화학회 1992 Applied Biological Chemistry (Appl Biol Chem) Vol.35 No.4
The plasmids pSUPBT and pSUPBTR were constructed with a vector pSUP2021 and the BT toxin gene in the plasmid pES 1. The plasmids constructed were introduced into the antagonistic rhizobacteria P. fluorescens KR164 by conjugation and P. fluoyescens having pSUPBT and pSUPBTR were named P. fluorescens KR164(pSUPBT) # 2, KR164(pSUPBT) # 3, KR164(pSUPBTR) # 2 and KR164(pSUPBTR) # 3, respectively. The BT toxin gene were identified in all transformants by Southern hybridization and the final product of BT toxin gene was identified only in P. fluorescens KR164(pSUPBTR) # 3 by SDS-PAGE. This crystal toxin protein were also observed in electron microscopy.
Synthesis of New Anthracyline Derivatives Containg Acetylsalicyclic or Palmitic Acid Moiety.
김완중,박시호,유동진,강흔수,정순량,Kim, Wan Jung,Park, Si Ho,Yu, Dong Jin,Gang, Heun Su,Jeong, Sun Ryang 대한화학회 2001 Bulletin of the Korean Chemical Society Vol.22 No.6
The potential anticancer agents new anthracycline derivatives (2~9) have been synthesized from daunomycin (1a) and doxorubicin (1b) ad starting materials. Compounds 2 and 6 were prepared by the nucleophilic displacement type esterification of a 14-bromodaunomycin (1c) with a acetylsalicylic and palmitic acid in triethylamine, respectively. Compound 3 and 7 were obtained from daunomycin (1a) by direct amidation with the corresponding acids in the presence of EDCI and PP as esterification regents. Whereas 4 and 8 were prepared by reaction of doxorubicin (1b) with one equivalent of acetylsalicylic and palmitic acid using DCC/DMAP, respectively, 5 and 9 were obtained from 1b by acylation with two equivalents of the corresponding acids using EDCI/PP reagents.
Pseudomonas fluorescens KR - 164 에 BT toxin gena 의 삽입과 식물 병원성 사상균에 미치는 영향
김영일,이영환,김종현,강흔수 한국농화학회 1991 Applied Biological Chemistry (Appl Biol Chem) Vol.34 No.2
고추, 오이 등의 시설원예 연작 토양에서 식물 병원성 사상균에 결항력을 갖는 Pseudomonas fluorescens KR-164의 chromosome에 BT toxin gene를 conjugation으로 삽입하여 형질변환된 P.fluorescens KR 164의 변이주를 조재하고, 모균주 (KR-164)와 형질 변환된 균주가 병원성 사상균, 작물의 발아율 및 암소배양에 미치는 영향 등을 검토하였으며 실험 결과를 요약하면 다음과 같다. 1. P.fluorescens KR-164에 BT toxin gene을 삽입하기 위하여 plasmid pES1과 pSUP2021을 이용하여 BT gene을 갖는 conjugation용 plasmid를 구성하였다. 2. Conjugation을 이용한 transposition으로 P.fluorescens KR-164의 choromosome에 위의 plasmid를 삽입시켜 KR-164가 형질변환된 P.fluorescens KR-164 변이주를 조제하였다. 3. 형질변환된 균주의 DNA를 Southern blotting으로 조사한 결과 모두 한 개 이상의 BT toxin gene이 삽입되었으며, BT toxin gene이 삽입된 chromosome의 위치가 균주마다 상이하였다. 4. 형질변환된 균주와 모균주의 생화학적 성질 등은 동일하였다. 5. 균주를 처리하여 오이에 대한 암소배양을 한 결과 무처리구에 비해 생육기간이 현저하게 연장되었다. 6. 균주가 배추 참깨의 발아율에 미치는 영향을 검정한 결과 무처리구에 비해 처리구에서 전반적으로 발아율이 향상되었다.
Synthesis of New Anthracycline Derivatives Including Butyric or Retinoic Acid Moiety.
김완중,박시호,유동진,강흔수,정순량,Kim, Wan Jung,Park, Si Ho,Yu, Dong Jin,Gang, Heun Su,Jeong, Sun Ryang 대한화학회 2001 Bulletin of the Korean Chemical Society Vol.22 No.6
The potential anticancer agents, new anthracycline analogues (2-9) have been synthesized from the glycosides daunomycin (1a) and doxorubicin (1b). Compounds 2 and 6 were prepared by nucleophilic displacement esterification of a 14-bromodauomycin(1c) with sodium or potassium salts of butyric and all trans retinoic acid, respectively. Compounds 3 and 7 were obtained from daunomycin (1a) by direct amidation with a butyric and all trans retinoic acid in the presence of EDCI and PP, respectively. Compounds 4 and 8 were obtained from doxorubicin (1b) by reaction with the corresponding acids in the same manner. Compounds 5 and 9 were prepared from doxorubicin (1b) by acylation with two equivalents of the corresponding acids under the same reaction conditions.
새로운 Morindaparvin A 유도체들의 합성 (Ⅱ)
박시호,로영쇠,권윤자,강흔수,조인호,Park, Si Ho,Rho, Young S.,Kwon, Yoon Ja,Kang, Heun Soo,Cho, In Ho 대한화학회 1995 대한화학회지 Vol.39 No.3
이미 합성하여 보고한 1,2-Dihydroxyanthraquinone 유도체(9, 10)에 매우 간편하고 효율적인 방법으로 $-N(CH_3)_2,\;-OMe,\;-NH_2,\;-COOH,\;-CHClC(O)NH_2,\;-CHClCH_2OH,\;-CHClC(O)CH_3$ 등이 붙은 여러 종류의 새로운 Morindaparvin A(2~8)의 유도체를 합성하였다. A simple and efficient synthesis of new kind of Morindaparvin A derivatives (2~8) containing $-N(CH_3)_2,\;-OMe,\;-NH_2,\;-COOH,\;-CHClC(O)NH_2,\;-CHClCH_2OH\;and\;-CHClC(O)CH_3$ was completed with the reported 1,2-dihydroxyanthraquinone derivatives (9, 10).
Metabolic loading of guanosine induces chondrocyte apoptosis via the Fas pathway
김동조,정준호,류은경,임정효,류윤식,박소현,김경태,강흔수,정홍근,박상철 생화학분자생물학회 2006 Experimental and molecular medicine Vol.38 No.4
Although the apoptosis of chondrocytes plays an important role in endochondral ossification, its mechanism has not been elucidated. In this study, we show that guanosine induces chondrocyte apop - tosis based on the results of acridine orange/ ethidium bromide staining, caspase-3 activation, and sub-G1 fraction analysis. The potent inhibitory effect of dipyridamole, a nucleoside transporter blocker, indicates that extracellular guanosine must enter the chondrocytes to induce apoptosis. We found that guanosine promotes Fas-Fas ligand interaction which, in turn, leads to chondrocyte apoptosis. These findings indicate a novel mechanism for endochondral ossification via metabolic regulation.
달맞이순 (Oenothera laciniata) 에탄올 추출물 섭취가 고지방식이로 유도한 비만 마우스에서 항산화 및 비만억제효과
곽충실,김미주,김선기,박순영,김인규,강흔수 한국영양학회 2019 Journal of Nutrition and Health Vol.52 No.6
Purpose: Sprouts of evening primrose (Oenothera laciniata, OL) were reported to have high contents of flavonoids and potent antioxidant activity. This study examined the antioxidant and antiobesity activities of OL sprouts to determine if they could be a natural health-beneficial resource preventing obesity and oxidative stress. Methods: OL sprouts were extracted with 50% ethanol, evaporated, and lyophilized (OLE). The in vitro antioxidant activity of OLE was examined using four different tests. The antiobesity activity and in vivo antioxidant activity from OLE consumption were examined using high fat diet-induced obese (DIO) C57BL/6 mice. Results: The IC 50 for the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging and superoxide dismutase (SOD)-like activities of OLE were 26.2 μg/mL and 327.6 μg/mL, respectively. OLE exhibited the ferric reducing antioxidant power (FRAP) activity of 56.7 μg ascorbic acid eq./mL at 100 μg/mL, and an increased glutathione level by 65.1% at 200 μg/mL compared to the control in the hUC-MSC stem cells. In an animal study, oral treatment with 50 mg or 100 mg of OLE/kg body weight for 14 weeks reduced the body weight gain, visceral fat content, fat cell size, blood leptin, and triglyceride levels, as well as the atherogenic index compared to the high fat diet control group (HFC) (p < 0.05). The blood malondialdehyde (MDA) level and the catalase and SOD-1 activities in adipose tissue were reduced significantly by the OLE treatment compared to HFC as well (p < 0.05). In epididymal adipose tissue, the OLE treatment reduced the mRNA expression of leptin, PPAR-γ and FAS significantly (p < 0.05) compared to HFC while it increased adiponectin expression (p < 0.05). Conclusion: OLE consumption has potent antioxidant and antiobesity activities via the suppression of oxidative stress and lipogenesis in DIO mice. Therefore, OLE could be a good candidate as a natural resource to develop functional food products that prevent obesity and oxidative stress.