사람 대뇌피질에서 Kappa Opioid 수용체의 이질성

소수미,한낙천,장전,은재순,김기원 의과학연구소 1996 全北醫大論文集 Vol.20 No.1

Experimental data have shown that there are considerable species differences in the distribution of opioid receptor types in mammalian brain. We have evaluated the properties of non-μ non-δ binding of [^3H]-diprenorphine([^3H]DIP), a nonselective opioid antagonist, in human cortex membranes. Bindings to μ and δ site were inhibited by the use of an excess of competing selective agonists, DAMGO(1μM) and DPDPE(1 μM), for these sites. Dynorphine-A(1-13), U-69, 593 and U-50, 488H, selective k_1 agonists, (-)ethyketocyclazocine and nor-binaltorphimine, k_1 and k_2 opioid ligands, inhibited the [^3H] DIP binding to human cortex membranes with high affinities. In the presence of cold ligands, I μM each of DAMGO, DPDPE and U-69, 593, that block μ-, δ-, and k_1 sites, poioid binding still remained. Specific binding of[^3H]DIP to this stie was not influenced by U-50, 488H or β-endorphin even in micromolar dose range, but inhibited y DIP, bremazocine, (-)eyhylketocylazocine and nor-binaltorphimine. The concentration-inhibition curves of these ligands in the presence of DAMGO and DPDPE were displaced further to right by the addition of U-69, 593. These data suggest that there are at least 2 kinds of k-opioid binding sites(k_1 and k_2) in human cortex.

Agrobacterium Mediated Transformation of Nicotiana glauca, a Woody Plant

Kim, Young Soon,Chae, Soo Cheon,Soh, Woong Young,Suh, Mi Chung,Hong, Choo Bong Korean Society of Molecular Biology 1993 Molecules and cells Vol.3 No.3

Agrobacterium Mediated Transformation of Nicotiana glauca, a Woody Plant

Kim, Young Soon,Chae, Soo Cheon,Soh, Woong Young,Suh, Mi Chung,Hong, Choo Bong 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

Nicotiana glauca is a fast growing perennial and bears thousands of seed every year. These characteristics could provide advantages of using N. glauca as a model system for the study of gene expression controls in woody plants. Leaf explants of N. glauca were cocultured with Agrobacterium tumefaciens strain LBA4404 carrying a plasmid pBI121 and in vitro regenerated. Regenerating cells showed strong resistance to kanamycin on the in vitro regeneration medium, and transformants were efficiently selected on the medium containing 300 mg/l of kanamycin. In vitro regenerated N. glauca plants showed specific northern signals for β-glucuronidase transcripts. The seeds obtained from the self-fertilized transformed plants showed a Mendelian pattern of inheritance in kanamycin-resistance. T_2 generation plants from the kanamycin-resistant seeds also showed specific northern signals for β-glucuronidase transcripts.

Ligand binding profiles of U-69 , 593-sensitive and-insensitive sites in human cerebral cortex membranes ; Evidence of Kappa opioid receptors heterogeneity

(Kee Won Kim),(Young A Eun),(Soo Mi Soh),(Jae Soon Eun),(Kyu Park Cho) 전북대학교 의과학연구소 1996 全北醫大論文集 Vol.20 No.2

LIGAND BINDING PROFILES OF U-69,593-SENSITIVE AND-INSENSITIVE SITES IN HUMAN CEREBRAL CORTEX MEMBRANES : EVIDENCE OF Kappa OPIOID RECEPTORS HETEROGENEITY

Kim, Kee-Won,Eun, Young-A,Soh, Soo-Mi,Eun, Jae-Soon,Cho, Kyu-Park 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

Receptor binding studies were performed to characterize the properties of subtypes of k opioid receptors in membrane preparations of human cerebral cortex. [^3H]U69,593 ([^3H]U69), a selective k_1-agonist, and [^3H]diprenorphine ([^3]DIP), a non-selective opioid antagonist, in the presence of l uM each of DAMGO, DPDPE and U-69 to block u-, 8-, and k_l-sites, labeled single population of binding sites, respectively. [^3H]U-69 binding sites (Kd= 3.8±0.2 nM, Bmax=6.3±0.2 fmol/mg protein) had a binding profile that correspond to k_l-receptor. That is, dynorphin A (1-13) (Dyn A), bremazocine (BZC), U50,488H (U50), (-)ethylketocyclazocine (EKC) and nor-binaltorphimine (nor-BNI) bound to this site with high affinities. [^3H]DIP labeled binding sites (Kd=7.3±0.2nM, Bmax=102±9 fmol/mg protein) that were not sensitive to U-50, but to BZC, EKC and nor-BNI. These results indicate that k_1 and k_2 opioid receptors exist in human cerebral cortex with different ligand binding profiles.

아세틸콜린 수용체 항체(Acetylcholine receptor autoantibody) 검사의 의의

유소연,임수연,백송란,서미혜,문형호,유선희,Yoo, Soh-Yeon,Lim, Soo-Yeon,Pack, Song-Ran,Seo, Mi-Hye,Moon, Hyung-Ho,You, Sun-Hee 대한핵의학기술학회 2011 핵의학 기술 Vol.15 No.1

아세틸콜린 수용체는 아세틸콜린에 대한 신경근 접합부의 수용체로 중증 근무력증에서는 이 수용체에 대한 자가항체인 아세틸콜린 수용체 항체가 생산되어 수용체 단백질과의 사이에 항원항체 결합체를 형성하기 때문에 신경흥분의 전달장애를 가져오는 것으로 알려져 있어 아세틸콜린 수용체 항체의 측정은 중증 근무력증의 진단에 중요하다. 중증 근무력증(Myasthenia Gravis)은 근육의 힘이 비정상적으로 약해지거나 피로해지는 병으로 안검하수, 복시, 근력약화 등을 나타내는 자가 면역성 질환이다. 모든 근무력증 환자에서 이 자가항체가 존재하는 것은 아니고, 전신성 근무력증 환자의 80~90% 가량이 발견되며 안근육에 국한된 근무력증의 71%가 검출된다. 그러나 선천성 근무력증에서는 검출되지 않는다. 아세틸콜린수용체 항체 농도와 임상적 중증도와의 상관관계는 없는 것으로 알려져 있다. 본 연구에서는 아세틸콜린 수용체 항체의 검사법을 소개함으로서 중증 근무력증의 진단에 유용한 정보를 제공하고자 하였다. 대상은 본원에 내원한 환자들 중 2010년 8월부터 9월까지 총 19명의 아세틸콜린 수용체 항체 검사가 의뢰된 검체를 대상으로 검사를 시행하였고, 측정키트는 R사의 키트로 면역침강법(immuno-precipitation)을 이용한 비경쟁반응법이다. cut off 값은 0.2 nmol/L로 결과값 중 음성값(negative)은 0.2 nmol/L미만이며, 양성값(positive)은 0.2 nmol/L 이상으로 판정한다. 검사를 시행한 19명의 환자 중 7명이 양성으로 보고되었으며(36.8%), 7명 중 6명이 근무력증(MG)으로 진단되었다. 중증 근무력증의 확진에는 이 검사법뿐만 아니라 다른 여러 가지 추가 검사들이 필요하다. 희귀병을 진단하는 검사법이라 많이 보편화된 검사는 아니지만 중증 근무력증은 아세틸콜린 수용체에 대한 항체가 생겨서 기능장애를 초래하는 질병이기 때문에 이 항체의 측정은 그의 진단에 유용할 것이라고 사료된다. Purpose: Acetylcholine receptor antibodies cause acetylcholine receptor loss, which is responsible for failure of the neuromuscular junction in the acetylcholine receptor autoantibody. The disease characterized by muscle weakness and fatigue, myasthenia gravis(MG) occurs when the body inappropriately produces antibodies against acetylcholine receptors, and thus inhibits proper acetylcholine signal transmission. And this reason, the measurement of acetylcholine receptor antibodies can be of considerable value in disease diagnosis. Methods: From 2010. August to September, we tested orderd AchRAb 19 samples to get the results. 1. Pipette $5{\mu}{\ell}$ undiluted patient sera and kit control and add 125I AChR $50{\mu}{\ell}$ and incubate at R.T for 2 hours. 2. Pipette $50{\mu}{\ell}$ of anti-human IgG into each tube, and incubate at $2{\sim}8^{\circ}C$ for 2 hours. 3. Pipette $25{\mu}{\ell}$ precipitation enhancer into each tube and add 1mL washing solution into all tubes. 4. Centrifuge each tube for 20minutes at $2{\sim}8^{\circ}C$ at 1500g. 5. Aspirate or decant the supernatant. 6. Pipette 1 mL washing solution into all tubes and resuspend the pellet and repeat centrifugation. 7. Aspirate or decant the supernatant and count all tubes on a gamma counter. Results: Cut off value is 0.2 nmol/L and the results taken below 0.2 nmol/L are negative, the results above that identified as being positive values. We assayed the 19 patients samples and got 7 positive results. Of which, 6 patients were diagnosed as MG.(85.7%). Conclusions: Acetylcholine Receptor autoantibody test is intended for use by persons only for the quantitative determination of it in human serum. Even if measurement of the antibodies is not a routine test, it can be of considerable value in disease diagnosis.

동시 병발한 그레이브스병과 중증근무력증의 수술적 치험 1예

정윤석,진윤미,소의영,김명욱,류기선,류한영,주인수 대한내분비학회 1997 Endocrinology and metabolism Vol.12 No.3

Graves disease occur in association with myasthenia gravis is rare. We report a case of Graves disease and myasthenia gravis treated by bilateral subtotal thyroidectomy and total thymectomy simultaneously. A 37 year old woman was admitted with anterior neck mass and ptosis. Various examinations were compatible with combined Graves disease and myasthenia gravis. The bilateral subtotal thyroidectomy and total thymectomy were done simultaneously. The pathologic diagnosis was Graves disease and thymic hyperplasia. The patients postoperative course was uneventful. The thyroid function of patient became euthyroid and the clinical symptoms related with myastenia gravis resolved during follow up period. (J Kor Soc Endocrinol 12:473-477, 1997)

Nonisotropic Propagation of Light in Human Forearm

Sang-Hyun Park,Chuho Choi,윤길원,Hak-Soo Shin,Joon-Mo Yang,Kwang-Sup Soh,Sun Mi Choi,Yeonhee Ryu 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.48 No.II

Light propagation in the skin of the human forearm was investigated in vivo and in situ. In this study, a sensitive photomultiplier tube with a spectral range of 300 650 nm was used to detect the extremely weak light that is propagated in skin from 17 mm up to 33 mm. We devised a control system that enabled us to guide the optical fibers so that the input and the detection could be exactly located on the skin in complete darkness. We obtained an effective attenuation coefficient whose values were 0.21 ± 0.03 cm.1 and 0.28 ± 0.06 cm.1 in the longitudinal and the transversal directions, respectively, over a long distance ( 17 mm). Those value were much smaller than the previously known value of 2.7 3.3 cm.1 for a short distance ( 2 mm). In addition, we found that light propagated better longitudinally than transverse, which contrasted with the isotropic propagation at short distances. These results might have significance for phototherapy. Light propagation in the skin of the human forearm was investigated in vivo and in situ. In this study, a sensitive photomultiplier tube with a spectral range of 300 650 nm was used to detect the extremely weak light that is propagated in skin from 17 mm up to 33 mm. We devised a control system that enabled us to guide the optical fibers so that the input and the detection could be exactly located on the skin in complete darkness. We obtained an effective attenuation coefficient whose values were 0.21 ± 0.03 cm.1 and 0.28 ± 0.06 cm.1 in the longitudinal and the transversal directions, respectively, over a long distance ( 17 mm). Those value were much smaller than the previously known value of 2.7 3.3 cm.1 for a short distance ( 2 mm). In addition, we found that light propagated better longitudinally than transverse, which contrasted with the isotropic propagation at short distances. These results might have significance for phototherapy.

Overexpression of PRE1 and its Homologous Genes Activates Gibberellin-dependent Responses in Arabidopsis thaliana

Lee, Sumin,Lee, Seunghee,Yang, Ki-Young,Kim, Young-Mi,Park, So-Yeon,Kim, Soo Young,Soh, Moon-Soo Oxford University Press 2006 Plant & cell physiology Vol.47 No.5

<P>Gibberellins control various aspects of growth and development. Here, we identified a gene, designated <I>paclobutrazol resistance1</I> (<I>PRE1</I>), by screening <I>Arabidopsis</I> activation-tagged lines. <I>PRE1</I> encodes a helix–loop–helix protein and belongs to a small gene family. Physiological and genetic analysis indicated that overexpression of <I>PRE1</I> altered various aspects of gibberellin-dependent responses such as germination, elongation of hypocotyl/petiole, floral induction and fruit development, and suppressed gibberellin-deficient phenotypes of the <I>ga2</I> mutant. Expression of some gibberellin-responsive genes was also affected by <I>PRE1</I>. Expression of <I>PRE1</I> was shown to be early gibberellin inducible in the wild-type plants and under control of SPY and GAI, upstream negative regulators of gibberellin signaling. The shortened hypocotyl length phenotype of the <I>gai-1</I> mutant was suppressed by <I>PRE1</I> overexpression. Ectopic overexpression of each of the four <I>PRE1</I>-related genes conferred pleiotropic phenotypes similar to <I>PRE1</I> overexpression, indicative of overlapping functions among the <I>PRE</I> gene family. Our results of gain-of-function studies suggest that <I>PRE</I> genes may have a regulatory role in gibberellin-dependent development in <I>Arabidopsis thaliana.</I></P>

백서와 기니픽의 대뇌피질에서 Opioid Kappa 수용체의 특성에 관한 연구

김기원(Kee-Won Kim),노혜원(Hye-Won Rho),김형일(Hyoung-Il Kim),은재순(Jae-Soon Eun),소수미(Soo-Mi Soh),조규박(Kyu-Park Cho) 대한약리학회 1994 대한약리학잡지 Vol.30 No.2

In this study, we tested the influences of several κ opioid ligands on the [³H]diprenorphine binding in rat and guinea pig cortex membrane preparations. Using paradigm to block μ and δ opioid receptors with DAMGO(1μM) and DPDPE(1μM), [³H]diprenorphine labeled κ sites. Competition analysis in both rat and guinea pig cortex has shown a single population of [³H]diprenorphine binding site with different Kd values, respectively. There is a significant difference in Ki values of (-) WIN44441 and (+)WIN44441 in both rat and guinea pig cortex. Bremazocine, (-)ethylketocyclazocine, (-)cyclazocine, nor-binaltorphimine effectively inhibited the [³H]diprenorphine binding with different Ki values in rat and guinea pig cortex. U-69,593, U-50,488H and dynorphine-A (1-8) did not inhibit the [³H]diprenorphine binding in rat but in guinea pig cortex. Nor-binaltorphimine was a ligand discriminate the κ₁, and κ₂ receptor most effectively. We, also, examined the influence of Na ion and GTPγS, a nonhydrolyzable guanine nucleotide analog, on the inhibition of [³H]diprenorphine binding by diprenorphine, (-)ethyl-ketocyclazocine, U-69,593 and bremazocine. By the replacement of NaCl with N-methy-D-glucamine or addition of GTPγS, Ki values of diprenorpnine were not changed and that of ethylketocyclazocine were changed significantly in both rat and guinea pig cortex. The Ki value of bremazocine was decreased by removal of Na ion, and increased by GTPγS, however, was not changed by any one of either. These results suggest that there are 2 kinds of subtypes of κ opioid receptor, κ₁, and κ₂, showing different Ki values for various κ opioid ligands, also, bremazocine possess the antagonistic property at κ₂ site which is dominant subtype of K receptor in rat cortex.