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Expression profiles of microRNAs in skeletal muscle of sheep by deep sequencing
Zhi-Jin Liu,Cun-Yuan Li,Xiao-Yue Li,Yang Yao,Wei Ni,Xiang-Yu Zhang,Yang Cao,Wureli Hazi,Dawei Wang,Renzhe Quan,Shuting Yu,Yuyu Wu,Songmin Niu,Yulong Cui,Yaseen Khan,Shengwei Hu 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.6
Objective: MicroRNAs are a class of endogenous small regulatory RNAs that regulate cell proliferation, differentiation and apoptosis. Recent studies on miRNAs are mainly focused on mice, human and pig. However, the studies on miRNAs in skeletal muscle of sheep are not comprehensive. Methods: RNA-seq technology was used to perform genomic analysis of miRNAs in prenatal and postnatal skeletal muscle of sheep. Targeted genes were predicted using miRanda software and miRNA-mRNA interactions were verified by quantitative real-time polymerase chain reaction. To further investigate the function of miRNAs, candidate targeted genes were enriched for analysis using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment. Results: The results showed total of 1,086 known miRNAs and 40 new candidate miRNAs were detected in prenatal and postnatal skeletal muscle of sheep. In addition, 345 miRNAs (151 up-regulated, 94 down-regulated) were differentially expressed. Moreover, miRanda software was performed to predict targeted genes of miRNAs, resulting in a total of 2,833 predicted targets, especially miR-381 which targeted multiple muscle-related mRNAs. Furthermore, GO and KEGG pathway analysis confirmed that targeted genes of miRNAs were involved in development of skeletal muscles. Conclusion: This study supplements the miRNA database of sheep, which provides valuable information for further study of the biological function of miRNAs in sheep skeletal muscle.
Seismic response of combined retaining structure with inclined rock slope
Yu-liang Lin,Jie Jin,Zhi-hao Jiang,Wei Liu,Hai-dong Liu,Rou-feng Li,Xiang Liu 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.84 No.5
A gravity wall combined with an anchoring lattice frame (a combined retaining structure) is adopted at a typical engineering site at Dali-Ruili Railway Line China. Where, the combined retaining structure supports a soil deposit covering on different inclined rock slopes. With an aim to investigate and compare the effects of inclined rock slopes on the response of combined retaining structure under seismic excitation, three groups of shaking table tests are conducted. The rock slopes are shaped as planar surfaces inclined at angles of 20°, 30°, and 40° with the horizontal, respectively. The shaking table tests are supplemented by dynamic numerical simulations. The results regarding the horizontal acceleration response, vertical acceleration response, permanent displacement mode, and axial anchor force are comparatively examined. The acceleration response is more susceptible to outer structural profile of combined retaining structure than to inclined angle of rock slope. The permanent displacement decreases when the inclined angle of the rock slope increases within a range of 20°-40°. A critical inclined angle of rock slope exists within a range of 20°-40°, and induces the largest axial anchor force in the combined retaining structure.
Yu Hai Wang,Zhe Dai,Chao Yue Zhang,Guo Wen Sun,Zhong Wei Lu,Xiu Ping Gao,Geng Zhi Sun,Wei Lan,Zhen Xing Zhang,Xiao Jun Pan,Jin Yuan Zhou 한국물리학회 2020 Current Applied Physics Vol.20 No.9
It was demonstrated that the electrochemical performance enhancements in KOH-activated carbon materials should be mainly due to the created polar oxygen-containing functional groups (OFGs, such as such as C–O, C–– O, –OH, and O–C–– O), while the role of each OFGs on the electrochemical enhancements is still unclear. In this work, KOH activation treatments were systematically conducted on carbon nanotubes (CNTs) to explore the role of each OFG on the performance enhancements of Li–S batteries (LSBs). Results showed that the capacity of activated-CNT-sulfur (a-CNT-S) cathodes is 33% higher than that of the pristine CNT-S cathodes, and their rate capability and cycling stability are also enhanced. And the electrochemical analysis combining with Fourier transform infrared spectroscopy indicated that the formed C–O bonds are the real factor for the enhanced electrochemical performances of a-CNT-S cathodes. Furthermore, the optimal activation conditions on CNTbased cathodes for LSBs were optimized to be 10 min at 700 ℃.
The effects of LNG-tank sloshing on the global motions of FLNG system
Zhi-Qiang Hu,Shu-Ya Wang,Gang Chen,Shu-Hong Chai,Yu-Ting Jin 대한조선학회 2017 International Journal of Naval Architecture and Oc Vol.9 No.1
This paper addresses a study of inner-tank sloshing effect on motion responses of a Floating Liquefied Natural Gas (FLNG) system, through experimental analysis and numerical modeling. To investigate hydrodynamic characteristics of FLNG under the conditions of with and without LNG-tank sloshing, a series of numerical simulations were carried out using potential flow solver SESAM. To validate the numerical simulations, model tests on the FLNG system was conducted in both liquid and solid ballast conditions with 75% tank filling level in height. Good correlations were observed between the measured and predicted results, proving the feasibility of the numerical modeling technique. On the verified numerical model, Response Amplitude Operators (RAOs) of the FLNG with 25% and 50% tank filling levels were calculated in six degrees of freedom. The influence of tank sloshing with varying tank filling levels on the RAOs has been presented and analyzed. The results showed that LNG-tank sloshing has a noticeable impact on the roll motion response of the FLNG and a moderate tank filling level is less helpful in reducing the roll motion response.
Analysis of Operating Efficiency of China’s Agricultural Listed Companies
Zhi-Run Li,Shi-Yong Piao,Yu-Cong Sun,Shuang-Yu HU,Xuan-You Jin,Jong-In Lee 경상대학교 농업생명과학연구원 2020 농업생명과학연구 Vol.54 No.6
In recent years, China's agricultural listed companies have developed rapidly. This paper studies the operating efficiency of listed agricultural companies in China. exploring the factors that affect the operating efficiency of listed agricultural companies, and proposes targeted countermeasures for the development of listed agricultural companies. And give some suggestions to Korean agricultural companies and A share investors. This paper uses the DEA model, selects the 40 best-developed Chinese agricultural listed companies in 2018 as a sample, analyzes the operating efficiency of these companies, and added two new input variables: asset impairment losses (AIL) and business tax and surcharge (BTS), which will also have an impact on operating efficiency. This paper can provide reliable suggestions for the development of agricultural listed companies, and thus guide the healthy operation of agricultural companies.
( Zhi-ke Liu ),( Qiu-yu Zhang ),( Ning-ning Yang ),( Ming-guo Xu ),( Jin-feng Xu ),( Ming-long Jing ),( Wen-xing Wu ),( Ya-dong Lu ),( Feng Shi ),( Chuang-fu Chen ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.3
Salmonellosis is a highly contagious bacterial disease that threatens both human and poultry health. Tests that can detect Salmonella in the field are urgently required to facilitate disease control and for epidemiological investigations. Here, we combined loop-mediated isothermal amplification (LAMP) with a chromatographic lateral flow dipstick (LFD) to rapidly and accurately detect Salmonella. LAMP primers were designed to target the Salmonella invA gene. LAMP conditions were optimized by adjusting the ratio of inner to outer primers, MgSO<sub>4</sub> concentration, dNTP mix concentration, amplification temperature, and amplification time. We evaluated the specificity of our novel LAMP-LFD method using six Salmonella species and six related non-Salmonella strains. All six of the Salmonella strains, but none of the non-Salmonella strains, were amplified. LAMP-LFD was sensitive enough to detect concentrations of Salmonella enterica subsp. enterica serovar Pullorum genomic DNA as low as 89 fg/μl, which is 1,000 times more sensitive than conventional PCR. When artificially contaminated feed samples were analyzed, LAMP-LFD was also more sensitive than PCR. Finally, LAMP-LFD gave no false positives across 350 chicken anal swabs. Therefore, our novel LAMP-LFD assay was highly sensitive, specific, convenient, and fast, making it a valuable tool for the early diagnosis and monitoring of Salmonella infection in chickens.
A damage localization method based on the singular value decomposition (SVD) for plates
Zhi-Bo Yang,Jin-Tao Yu,Shao-Hua Tian,Xue-Feng Chen,Guan-Ji Xu 국제구조공학회 2018 Smart Structures and Systems, An International Jou Vol.22 No.5
Boundary effect and the noise robustness are the two crucial aspects which affect the effectiveness of the damage localization based on the mode shape measurements. To overcome the boundary effect problem and enhance the noise robustness in damage detection, a simple damage localization method is proposed based on the Singular Value Decomposition (SVD) for the mode shape of composite plates. In the proposed method, the boundary effect problem is addressed by the decomposition and reconstruction of mode shape, and the noise robustness in enhanced by the noise filtering during the decomposition and reconstruction process. Numerical validations are performed on plate-like structures for various damage and boundary scenarios. Validations show that the proposed method is accurate and effective in the damage detection for the two-dimensional structures.