A Fermentation Strategy for Anti-MUC1 C595 Diabody Expression in Recombinant Escherichia Coli

Lan, John Chi-Wei,Ling, Tau Chuan,Hamilton, Grant,Lyddiatt, Andrew The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.5

The development of fermentation conditions for the production of C595 diabody fragment (dbFv) in E. coli HB2151 clone has been explored. Investigations were carried out to study the effect of carbon supplements over the expression period, the comparison of C595 dbfv production in synthetic and complex media, the influence of acetic acid upon antibody production, and comparison of one-stage and two-stage processes operated at batch or fed-batch modes in bioreactor. Yeast extract supplied during expression yielded more antibody fragment than any other carbon supply. The synthetic medium presented higher specific productivity (0.066 mg dbFv $g^{-1}$ dry cell weight) when compared to the complex medium (0.044 mg dbFv $g^{-1}$ DCW). The comparison of fermentation strategies demonstrated that (1) one-stage fed-batch fermentation performed higher C595 dbFv production than that operated in batch mode which was significantly affected by acetate concentration; (2) a two-stage batch operation could enhance C595 dbFv production. It was found that a concentration of 12.3 mg $L^{-1}$ broth of C595 dbFv and a cell concentration of 10.8g $L^{-1}$ broth were achieved at the end of two-stage operation in 5-L fermentation.

Efficacy and Safety of Sorafenib for Advanced Non-Small Cell Lung Cancer: a Meta-analysis of Randomized Controlled Trials

Wang, Wei-Lan,Tang, Zhi-Hui,Xie, Ting-Ting,Xiao, Bing-Kun,Zhang, Xin-Yu,Guo, Dai-Hong,Wang, Dong-Xiao,Pei, Fei,Si, Hai-Yan,Zhu, Man Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: Many clinical trials have been conducted to evaluate sorafenib for the treatment of advanced NSCLC, but the results for efficacy have been inconsistent. The aim of this study was to evaluate the efficacy and safety of sorafenib in patients with advanced NSCLC in more detail by meta-analysis. Methods: This meta-analysis of randomized controlled trials (RCTs) was performed after searching PubMed, EMBASE, ASCO Abstracts, ESMO Abstracts, and the proceedings of major conferences for relevant clinical trials. Two reviewers independently assessed the quality of the trials. Outcomes analysis were disease control rate (DCR), progression- free survival (PFS), overall survival (OS) with 95% confidence intervals (CI) and major toxicity. Subgroup analysis was conducted according to sorafenib monotherapy, in combination with chemotherapy or EGFR-TKI to investigate the preferred therapy strategy. Results: Results reported from 6 RCTs involving 2, 748 patients were included in the analysis. Compared to sorafenib-free group, SBT was not associated with higher DCR (RR 1.31 (0.96- 1.79), p=0.09), PFS (HR 0.82 (0.66-1.02), p=0.07) and OS (HR 1.01 (0.92-1.12), p=0.77). In terms of subgroup results, sorafenib monotherapy was associated with significant superior DCR and longer PFS, but failed to show advantage with regard to OS. Grade 3 or greater sorafenib-related adverse events included fatigue, hypertension, diarrhea, oral mucositis, rash and HFSR. Conclusions: SBT was revealed to yield no improvement in DCR, PFS and OS. However, sorafenib as monotherapy showed some activity in NSCLC. Further evaluation may be considered in subsets of patients who may benefit from this treatment. Sorafenib combined inhibition therapy should be limited unless the choice of platinum-doublet regimen, administration sequence or identification of predictive biomarkers are considered to receive better anti-tumor activity and prevention of resistance mechanisms.

Expression of the Pokemon Proto-oncogene in Nasopharyngeal Carcinoma Cell Lines and Tissues

Jiao, Wei,Liu, Fei,Tang, Feng-Zhu,Lan, Jiao,Xiao, Rui-Ping,Chen, Xing-Zhou,Ye, Hui-Lan,Cai, Yong-Lin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

To study the differentiated expression of the proto-oncogene Pokemon in nasopharyngeal carcinoma (NPC) cell lines and tissues, mRNA and protein expression levels of CNE1, CNE2, CNE3 and C666-1 were detected separately by reverse transcription polymerase chain reaction (RT-PCR), real-time PCR and Western-blotting. The immortalized nasopharyngeal epithelial cell line NP69 was used as a control. The Pokemon protein expression level in biopsy specimens from chronic rhinitis patients and undifferentiated non keratinizing NPC patients was determined by Western-blotting and arranged from high to low: C666-1>CNE1>CNE2> CNE3>NP69. The Pokemon mRNA expression level was also arranged from high to low: CNE1>CNE2>NP69>C666-1>CNE3. Pokemon expression of NP69 and C666-1 obviously varied from mRNA to protein. The Pokemon protein level of NPC biopsy specimens was obviously higher than in chronic rhinitis. The data suggest that high Pokemon protein expression is closely associated with undifferentiated non-keratinizing NPC and may provide useful information for NPC molecular target therapy.

Multicolour carbon dots with excitation‑independent emission by microwave solvothermal reaction

Xiao‑Lan Wei,Qin‑Ling Shi,Lan Jiang,Yu Qin 한국탄소학회 2023 Carbon Letters Vol.33 No.1

Carbon dots (CDs) with tunable fluorescence emissions have been developed from a wide range of small organic molecules with various bottom-up syntheses. However, most of them were prepared under high temperatures and high pressures with long reaction times and tedious purification processes. In addition, previously reported carbon dots frequently displayed excitation-dependent emissions, which restrict their further applications. Herein, we present a simple and rapid microwaveassisted solvothermal synthesis of multicolour carbon dots with excitation-independent emissions. In ethylene glycol, the green (G)-CDs emitting at 537 nm with a quantum efficiency (QY) of 15% were obtained by using a single precursor of phloroglucinol, and blue (B)- and yellow (Y)-CDs emitting at 436 nm and 557 nm with QYs of 55% and 28% were derived with additives of o- and m-phenylenediamine, respectively. Analyses of their chemical structures and optical processes suggest that highly polymeric carbon dots were uniformly formed from the small molecules and their fluorescences were predominantly originated from rapid direct recombination. Furthermore, emissions at different wavelengths were mainly attributed to different degrees of oxidation (13.9%, 15.2% and 16.4% oxygen in B-, G- and Y-CDs, respectively) and different proportions of pyrrolic nitrogen (10.4% and 1.40% in B- and Y-CDs, respectively). To demonstrate the application feasibility, the obtained carbon dots were utilized for ion detection and anti-counterfeiting. Based on static quenching of the carbon dots’ fluorescence, micro amounts of ferric ion in water samples were detected selectively and reproducibly. Moreover, the anti-counterfeiting pattern constructed by the carbon dots emitted fluorescence under ultraviolet illumination, but concealed perfectly under daylight. This achievement is of great potential for developing multicolour carbon dots of high qualities.

Association of the Single-Nucleotide Polymorphism and Haplotype of the Complement Receptor 1 Gene with Malaria

Yan Lan,Le-Gen Nong,Chuan-Dong Wei,Wen-Cheng Chen,Jun-Li Wang,Chun-Fang Wang,Guo-Gang Pan,Ye-Sheng Wei 연세대학교의과대학 2015 Yonsei medical journal Vol.56 No.2

Purpose: Although the polymorphisms of erythrocyte complement receptor type 1 (CR1) in patients with malaria have been extensively studied, a question of whether the polymorphisms of CR1 are associated with severe malaria remains controversial. Furthermore, no study has examined the association of CR1 polymorphisms with malaria in Chinese population. Therefore, we investigated the relationship of CR1 gene polymorphism and malaria in Chinese population. Materials and Methods:We analyzed polymorphisms of CR1 gene rs2274567 G/A, rs4844600 G/A, and rs2296160 C/T in 509 patients with malaria and 503 controls, using the Taqmangenotyping assay and PCR-direct sequencing. Results: There were no significantdifferences in the genotype, allele and haplotype frequencies of CR1 gene rs2274567 G/A, rs4844600 G/A, and rs2296160 C/T polymorphisms between patientswith malaria and controls. Furthermore, there was no association of polymorphismsin the CR1 gene with the severity of malaria in Chinese population. Conclusion:These findings suggest that CR1 gene rs2274567 G/A, rs4844600 G/A, and rs2296160 C/T polymorphisms may not be involved in susceptibility to malariain Chinese population.

Protection of the hematopoietic system against radiation-induced damage: drugs, mechanisms, and developments

Yuanyun Wei,Yaqi Gong,Shuang Wei,Yonglin Chen,Jian Cui,Xiang Lin,Yueqiu Yu,Hongxia Yan,Hui Qin,Lan Yi 대한약학회 2022 Archives of Pharmacal Research Vol.45 No.8

Sometimes, people can be exposed to moderateor high doses of radiation accidentally or through the environment. Radiation can cause great harm to several systemswithin organisms, especially the hematopoietic system. Severaltypes of drugs protect the hematopoietic system againstradiation damage in diff erent ways. They can be classifi edas “synthetic drugs” and “natural compounds.” Their cellularmechanisms to protect organisms from radiation damageinclude free radical-scavenging, anti-oxidation, reducinggenotoxicity and apoptosis, and alleviating suppression ofthe bone marrow. These topics have been reviewed to providenew ideas for the development and research of drugsalleviating radiation-induced damage to the hematopoieticsystem.

Direct Purification of Burkholderia Pseudomallei Lipase from Fermentation Broth Using Aqueous Two-Phase Systems

Chien Wei Ooi,Beng Ti Tey,Siew Ling Hii,Arbakariya Ariff,Ho Shing Wu,John Chi Wei Lan,Ruey Shin Juang,Siti Mazlina Mustapa Kamal,Tau Chuan Ling 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.6

An aqueous two-phase purification process was employed for the recovery of Burkholderia pseudomallei from fermentation broth. The partition behavior of B.pseudmallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts. Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study An aqueous two-phase purification process was employed for the recovery of Burkholderia pseudomallei from fermentation broth. The partition behavior of B.pseudmallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts. Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study

Plenary Lecture 2 : The differentiation of embryonic stem cells and induced pluripotent stem cells to ovarian granulosa cells in human

( Chen Wei Lan ),( Mei Jou Chen ),( Pey Shynan Jan ),( Hsin Fu Chen ),( Hong Nerng Ho ) 대한산부인과학회 2014 서울심포지움 Vol.19 No.-

Impact of climate change in marine ecosystems on the spatiotemporal age structure of tuna population in the Pacific Ocean

Kuo-Wei Lan,Yan-Lun Wu,Ting-Yu Liang,Lu-Chi Chen 한국수산과학회 양식분과 2022 한국수산과학회 양식분과 학술대회 Vol.2022 No.10

A Fermentation Strategy for Anti-MUC1 C595 Diabody Expression in Recombinant Escherichia coli

John Chi-Wei Lan,Tau Chuan Ling,Grant Hamilton,Andrew Lyddiatt 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.5

The development of fermentation conditions for the production of C595 diabody fragment (dbFv) in E. coli HB2151 clone has been explored. Investigations were carried out to study the effect of carbon supplements over the expression period, the comparison of C595 dbfv production in synthetic and complex media, the influence of acetic acid upon antibody production, and comparison of one-stage and two-stage processes operated at batch or fed-batch modes in bioreactor. Yeast extract supplied during expression yielded more antibody fragment than any other carbon supply. The synthetic medium presented higher specific productivity (0.066 mg dbFv g-1 dry cell weight) when compared to the complex medium (0.044 mg dbFv g-1 DCW). The comparison of fermentation strategies demonstrated that (1) one-stage fed-batch fermentation performed higher C595 dbFv production than that operated in batch mode which was significantly affected by acetate concentration; (2) a two-stage batch operation could enhance C595 dbFv production. It was found that a concentration of 12.3 mg L-1 broth of C595 dbFv and a cell concentration of 10.8 g L-1 broth were achieved at the end of two-stage operation in 5-L fermentation.