Canonical transient receptor potential channels and their modulators: biology, pharmacology and therapeutic potentials

Yuan-Yuan Gao,Wen Tian,Hui-Nan Zhang,Yang Sun,Jing-Ru Meng,Wei Cao,Xiao-Qiang Li 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.4

Canonical transient receptor potential channels(TRPCs) are nonselective, high calcium permeability cationicchannels. The TRPCs family includes TRPC1, TRPC2,TRPC3, TRPC4, TRPC5, TRPC6, and TRPC7. These channelsare widely expressed in the cardiovascular and nervoussystems and exist in many other human tissues and celltypes, playing several crucial roles in the human physiologicaland pathological processes. Hence, the emergenceof TRPCs modulators can help investigate these channels’applications in health and disease. It is worth noting that theTRPCs subfamilies have structural and functional similarities,which presents a signifi cant diffi culty in screening anddiscovering of TRPCs modulators. In the past few years,only a limited number of selective modulators of TRPCswere detected; thus, additional research on more potent andmore selective TRPCs modulators is needed. The presentreview focuses on the striking desired therapeutic eff ectsof TRPCs modulators, which provides intel on the structuralmodifi cation of TRPCs modulators and further pharmacologicalresearch. Importantly, TRPCs modulators cansignifi cantly facilitate future studies of TRPCs and TRPCsrelated diseases.

Physicochemical Characterization and an Injection Formulation Study of Water Insoluble ZCVI4-2, a Novel NO-Donor Anticancer Compound

Yuan Gao,Jianjun Zhang,Li Li,Feng Su,Zhenhua Gong,Yisheng Lai,Yihua Zhang 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.7

ZCVI4-2 was a novel nitric oxide-releasing glycosyl derivative of oleanolic acid that displayed strong cytotoxicity selectively against human hepatocellular carcinoma in vitro and in vivo. In this study, ZCVI4-2 was characterized by FT-IR spectroscopy, differential scanning calorimetry,powder X-ray diffractometry, Raman spectroscopy, hygroscopicity and stability. A high performance liquid chromatography method was also established for the quantitative determination of solubility and additional stability profile of ZCVI4-2. ZCVI4-2 was found to be an amorphous and stable solid with low solubility of less than 10 μg/mL. Based on the solubilization tests that included methods of cosolvency and micellization, the solution mixture of 5% Solutol HS-15, 5% 1, 2-propylene glycol and 5% anhydrous ethanol was determined to be the system for the preparation of the ZCVI4-2 early injection solution. The effect of pH, temperature, light and injectable isotonic glucose or NaCl solution on ZCVI4-2 injection was also investigated. Good stability was observed at all testing conditions. Under the conditions studied, the NO-releasing rate and amount of ZCVI4-2 from the early injection solution in rat plasma demonstrated a promising therapeutic efficacy while maintaining a good safety profile.

Adaptive Cooperation for Bidirectional Communication in Cognitive Radio Networks

( Yuan Gao ),( Changping Zhu ),( Zhixiang Deng ),( Yibin Tang ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.3

In the interweave cognitive networks, the interference from the primary user degrades the performance of the cognitive user transmissions. In this paper, we propose an adaptive cooperation scheme in the interweave cognitive networks to improve the performance of the cognitive user transmissions. In the proposed scheme for the bidirectional communication of two end-source cognitive users, the bidirectional communication is completed through the non-relay direct transmission, the one-way relaying cooperation transmission, and the two-way relaying cooperation transmission depending on the limited feedback from the end-sources. For the performance analysis of the proposed scheme, we derive the outage probability and the finite-SNR diversity multiplexing tradeoff (f-DMT) in a closed form, considering the imperfect spectrum sensing, the interference from the primary user, and the power allocation between the relay and the end-sources. The results show that compared with the direct transmissions (DT), the pure one-way relaying transmissions (POWRT), and the pure two-way relaying transmissions (PTWRT), the proposed scheme has better outage performance. In terms of the f-DMT, the proposed scheme outperforms the full cooperation transmissions of the POWRT and PTWRT.

An Optimization Method for Designing LDPC Analog Decoders Based on Frequent Subgraph Mining Algorithm

Yuan Gao,Yujie Lin,Jibo Dai 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.10

To lower the mapping complexity of designing analog decoders, a method to optimize the design of low-density parity-check (LDPC) analog decoders is proposed in this paper. Based on factor graphs and the sum-product algorithm, the LDPC decoding process on the factor graph and the construction of analog decoders are exploited. Then the frequent subgraph mining algorithm is introduced to search the isomorphic subgraphs in factor graphs. According to the output of the frequent subgraph mining algorithm which enumerates all the subgraphs in factor graphs, the mapping complexity of a LDPC analog decoder can be significantly reduced. Finally, a (40, 16) LDPC analog decoder is constructed using the proposed method. Simulation results show that the need to place gates and connections can be reduced 90% and 23%, respectively, and the ideal performance is obtained by carefully choosing unit currents and decoding time.

The use of fluorescent techniques in combination with flow cytometry for fast counting of Bifidobacterium longum ATCC BAA-2753 in BIFICO capsule

Yuan Gao,Hong-jing Yu,Bin Wen 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.5

BIFICO is a main microecological drug that the main ingredient is Bifidobacterium longum ATCC BAA- 2753. It’s necessary to detect the number of Bifidobacterium longum ATCC BAA-2753 for quality control during the production of BIFICO. Plate count assay (PCA) is the most commonly used method for counting microbial cells. However, not all microorganisms can be counted by PCA. (e.g. uncultured microorganisms under specific growth conditions). In this study, a method of fluorescent techniques in combination with flow cytometry (FCM) was established for the counting of Bifidobacterium longum BAA-2753. Using SYTO9 and PI stain, flow cytometric analysis could easily differentiate live bacteria and dead bacteria. The number of live bacteria determined by FCM was about fourfold higher than that counted by PCA. Therefore, the FCM method could be a practical tool for the quality control of Bifidobacterium longum BAA-2753 in the industrial production of BIFICO.

Research on reliability of centrifugal compressor unit based on dynamic Bayesian network of fault tree mapping

Gao Yuan,Zhang Liang,Zhou Jiawei,Wei Bojia,Yan Zhongchao 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.5

For the first time, the fault tree mapping dynamic Bayesian network (DBN) method is applied to the reliability research study of centrifugal compressor units, and its usability and reliability are evaluated dynamically. First, the fault data using the K-means method eliminates abnormal data and builds a fault tree model. The structure is mapped to the DBN structure to complete the structure learning, and the logic gate is mapped to the conditional probability to achieve the parameter learning. Then the model is solved bidirectionally. The change rule of system node reliability with time is solved forward, and the posterior probability of the system node is solved reversely to complete fault diagnosis. Finally, Monte Carlo simulation analysis and the Markov process verify the dynamic reliability and steady-state availability. The results show that the Monte Carlo simulation method is almost consistent with the reliability prediction curve of the DBN model. Indicating that the accuracy of the DBN model is reliable and the computational efficiency is improved by about 81434 times. The steady-state availability calculated using the DBN model is approximately 0.99963, which is close to reality compared to the Markov process. This method can better describe centrifugal compressor units’ dynamic reliability and maintainability and provide decision support for regular maintenance of essential parts and enterprise procurement.

Combined pre-detection and sleeping for energy-efficient spectrum sensing in cognitive radio networks

Gao, Yuan,Deng, Zhixiang,Choi, Dongmin,Choi, Chang Elsevier 2018 Journal of parallel and distributed computing Vol.114 No.-

<P><B>Abstract</B></P> <P>In this paper, we propose a cooperative spectrum sensing scheme including a combined pre-detection and sleeping policy. In the scheme, the designed pre-detection sub-phase is applied at the beginning of the detection of the presence of a primary user, where all sensing nodes are involved to improve the detection performance. The sleeping policy is applied for each sensing node respectively at the end of the pre-detection sub-phase and the beginning of the transmission of the local detection results, in order to decrease sensing energy consumption. We formulate the problem of minimizing the maximum average energy consumption per sensing node in Rayleigh fading channels, considering the constraints of the required global detection and false alarm probabilities and the tolerable interference caused to the primary user. Numerical results show that the scheme achieves significant energy saving as compared to a scheme that includes only a sleeping policy and does not consider pre-detection.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We propose a combined pre-detection and sleeping scheme for spectrum sensing. </LI> <LI> The sensing performance is not affected by a high sleeping rate of secondary relays. </LI> <LI> An analytical framework is based on more practical channel conditions. </LI> <LI> A search algorithm is studied to obtain low complexity for solving the optimal problem. </LI> <LI> The proposed scheme achieves significant energy saving for spectrum sensing. </LI> </UL> </P>

Optimization the extraction of anthocyanins from blueberry residue by dual-aqueous phase method and cell damage protection study

Yuan Gao,Yubin Ji,Fuling Wang,Wenlan Li,Xiaomeng Zhang,Zhihui Niu,Zhuo Wang 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.13

Blueberry residue is usually discarded as waste,but has a high anthocyanins content. The extraction methodof anthocyanins from blueberry residue with ultrasonicassisted dual-aqueous phase system was optimized. Interms of the principle of central group and design (CCD)experimental design, three-factor and five-level responsesurface analysis was adopted to optimize the extractionconditions with the extraction rate of anthocyanins. Theoptimum extraction rate of anthocyanin was12.372 ± 0.078 mg/g. Anthocyanin extract could protectthe pBR322 DNA oxidative damage induced by Fentonreagent, increase the superoxide dismutase(SOD) and glutathioneperoxidase (GSH-Px) enzyme activities, anddecrease the H2O2-induced cell apoptosis of human normalliver cell (LO2 cell). The study indicates that the extractionrate of anthocyanin was increased by optimized ultrasonicassisted dual-aqueous phase system. The anthocyaninextract could protect DNA and LO2 cell from oxidativedamage.

Kinesin Family Member 11 Enhances the Self-Renewal Ability of Breast Cancer Cells by Participating in the Wnt/β-Catenin Pathway

Yuan-yuan Pei,Gao-chi Li,Jian Ran,Xin-hong Wan,Feng-xiang Wei,Lan Wang 한국유방암학회 2019 Journal of breast cancer Vol.22 No.4

Purpose: Our previous studies have shown that kinesin family member 11 (KIF11) is markedly overexpressed in human breast cancer cells or tissues and positively correlated with distant metastasis and prognosis in patients with breast cancer, suggesting an important role in the regulation of cancer stem cells. Herein, we examined the role of KIF11 in breast cancer stem cells. Methods: In the current study, we validated our previous findings through analysis of data collected in The Cancer Genome Atlas. Endogenous KIF11 was stably silenced in MCF-7 and SKBR-3 cells. Flow cytometry was used to measure the proportion of side-population (SP) cells. Mammosphere culture and tumor implantation experiments in immunodeficient mice were used to assess the self-renewal ability of breast cancer cells. Real-time polymerase chain reaction, western blot, immunofluorescence staining, luciferase reporter assays and Wnt agonist treatment were conducted to investigate the signaling pathways regulated by KIF11. Results: We found that the expression level of KIF11 was positively correlated with stem cell-enrichment genes. The proportion of SP cells was significantly reduced in KIF11-silenced cells. Silencing endogenous KIF11 not only reduced the size and number of mammospheres in vitro, but also reduced the ability of breast cancer cells to form tumors in mice. Simultaneously, we found that KIF11 was involved in regulating the activation of the Wnt/β-catenin signaling pathway. Conclusion: Endogenous KIF11 enhances the self-renewal of breast cancer cells by activating the Wnt/β-catenin signaling pathway, thereby enhancing the characteristics of breast cancer stem cells.

Punicic Acid from Trichosanthes kirilowii Seed Oil Is Rapidly Metabolized to Conjugated Linoleic Acid in Rats

Gao-Feng Yuan,Jing-Qun Yuan,Duo Li 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.2

The incorporation and metabolism of orally administered punicic acid (PA), one isomer of conjugated linolenic acid (CLNA), in rat tissues and plasma were studied over a 24-hour period. The punic acid was derived from Trichosanthes kirilowii Maxim seed oil, a unique PA-containing material, and identified and analyzed by high-performance liquid chromatography and gas chromatography-mass spectrometry. The results show that PA was incorporated and metabolized to 9c,11t-conjugated linoleic acid (CLA) in rat plasma, liver, kidney, heart, brain, and adipose tissue. The level of PA and CLA in liver and plasma was higher than in brain, heart, kidney, and adipose tissue, and the lowest accumulation occurred in the brain. The observation that PA can be converted into 9c,11t-CLA has gained increased importance since it has been demonstrated that 9c,11t-CLA exerts many biological activities. Therefore natural resources containing CLNA, especially edible T. kirilowii seed, could be a potential dietary source of CLA, following PA metabolism. PA is expected to be used as a functional food and nutraceutical.