Endophytic Fungi from Dalbergia odorifera T. Chen Producing Naringenin Inhibit the Growth of Staphylococcus aureus by Interfering with Cell Membrane, DNA, and Protein

Yuan Gao,Yubin Ji,Wenlan Li,Fuling Wang,Fuling Wang,Xiaomeng Zhang,Zhihui Niu,Lulu Zhou,Lijun Yan 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.2

This study focused on the antibacterial effects of the endophytic fungi producing naringenin from Dalbergia odorifera T. Chen against Staphylococcus aureus. The antibacterial activity was measured by the inhibition diameters, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). The time-killing curve was also used to evaluate its antibacterial efficacy. The results of antibacterial activity determinations showed that endophytic fungi secondary metabolites can inhibit the growth of five pathogenic bacteria (S. aureus, Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, and Bacillus subtilis) and the most sensitive strain was S. aureus that had the MIC and MBC values of 0.13 and 0.50 mg/mL, respectively. The membrane permeability study was measured by a DNA leakage assay and electrical conductivity assay. Furthermore, the whole-cell protein lysates and DNA fragmentation assay was evaluated. The morphology of S. aureus treated with the endophytic fungi products was observed by scanning electron microscopy (SEM). The probable antibacterial mechanism of endophytic fungi secondary metabolites was the increased membrane permeability that leads to leaks of nucleic acids and proteins. SEM results further confirmed that the extracts can interfere with the integrity of S. aureus cell membrane and further inhibit the growth of bacteria, resulting in the death of bacteria. This study provides a new perspective for the antibacterial functions of endophytic fungi secondary metabolites for biomedical applications.

The Chinese Version of the Work Control Scale for Nurses: Modifying the Translation and Psychometric Testing

Wen Fule,Wang Lin,Fang Zhiyin,Zhu Jinjin,Zhang Yaqing 한국간호과학회 2020 Asian Nursing Research Vol.14 No.2

Purpose: The aim of the study was to modify and test the psychometric properties of the Chinese version of the work control scale (C-WCS). Methods: The translated scale was administered to 840 nurses in Shanghai. Validity was assessed in terms of content validity and construct validity using exploratory factor analysis and confirmatory factor analysis. Internal consistency and testeretest reliability were estimated using Cronbach a and the intraclass correlation coefficient. Results: Psychometric analyses of the C-WCS indicate high reliability and good content and construct validity. Conclusion: The C-WCS has good psychometric properties and can be used as a valid tool for measuring work control among nurses in China. The C-WCS will help to further explore the correlations between perceived work control and organizational quality indicators such as nurses' satisfaction, job stress, wellbeing, or intention to stay. It can also be used in nursing outcome studies of work control strategies.

Preparation and characterization of boron films used for boron-lined gaseous neutron detectors

Deng Chao,Wang Qibiao,Wu Yadong,Peng Shuming,Liu Fule,Li Huailiang,Cheng Jianfeng,Tuo Xianguo 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.79 No.7

Boron-lined gaseous neutron detectors are being widely used in neutron detection to replace 3He proportional counters, and the boron film’s parameters comprise the key factors influencing the performance of such detectors. However, the method of characterizing boron film is relatively simple at present. In this study, boron films stuck to ultrathin glass substrate with different mass proportions of epoxy to natural boron (MPENBs) were prepared. A variety of characterization methods, including scanning electron microscopy, energy-dispersive X-ray spectroscopy, white-light interferometry, and multiple tape tests, were used to test the boron films simultaneously, and the test results are discussed herein. Moreover, neutron imaging was conducted to analyze the uniformity of boron-10 atoms. These characterization results demonstrate that the optimized MPENB formulation is 0.16 with the boron atomic ratio of chemical elements (ARCE) at approximately 68.8% and surface roughness Sa = 1.457 μm and that the structure of boron film is uniform and fluffy, contributing to improving the boron-lined method.

Optimization the extraction of anthocyanins from blueberry residue by dual-aqueous phase method and cell damage protection study

Yuan Gao,Yubin Ji,Fuling Wang,Wenlan Li,Xiaomeng Zhang,Zhihui Niu,Zhuo Wang 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.13

Blueberry residue is usually discarded as waste,but has a high anthocyanins content. The extraction methodof anthocyanins from blueberry residue with ultrasonicassisted dual-aqueous phase system was optimized. Interms of the principle of central group and design (CCD)experimental design, three-factor and five-level responsesurface analysis was adopted to optimize the extractionconditions with the extraction rate of anthocyanins. Theoptimum extraction rate of anthocyanin was12.372 ± 0.078 mg/g. Anthocyanin extract could protectthe pBR322 DNA oxidative damage induced by Fentonreagent, increase the superoxide dismutase(SOD) and glutathioneperoxidase (GSH-Px) enzyme activities, anddecrease the H2O2-induced cell apoptosis of human normalliver cell (LO2 cell). The study indicates that the extractionrate of anthocyanin was increased by optimized ultrasonicassisted dual-aqueous phase system. The anthocyaninextract could protect DNA and LO2 cell from oxidativedamage.

A Fully Integrated SoC for Smart Capsule Providing In-Body Continuous pH and Temperature Monitoring

Heng Liu,Hanjun Jiang,Jingpei Xia,Zhexiang Chi,Fule Li,Zhihua Wang 대한전자공학회 2016 Journal of semiconductor technology and science Vol.16 No.5

This paper presents a SoC (System-on-a-Chip) dedicated for a single-chip smart capsule which can be used to continuously monitor human alimentary canal pH and temperature values. The SoC is composed of the pH and temperature sensor interface circuit, a wireless transceiver, the power management circuit and the flow control logic. Fabricated in 0.18 μm standard CMOS technology, the SoC occupies a die area of ~9 mm². The SoC consumes 6.15 mW from a 3 V power supply, guaranteeing the smart capsule battery life is no less than 24 hours when using 50 mAh coin batteries. The experimental results show that measurement accuracy of the smart capsule is ±0.1 pH and ±0.2°C for pH and temperature sensing, respectively, which meets the requirement of in-body pH and temperature monitoring in clinical practice.

A Fully Integrated SoC for Smart Capsule Providing In-Body Continuous pH and Temperature Monitoring

Liu, Heng,Jiang, Hanjun,Xia, Jingpei,Chi, Zhexiang,Li, Fule,Wang, Zhihua The Institute of Electronics and Information Engin 2016 Journal of semiconductor technology and science Vol.16 No.5

This paper presents a SoC (System-on-a-Chip) dedicated for a single-chip smart capsule which can be used to continuously monitor human alimentary canal pH and temperature values. The SoC is composed of the pH and temperature sensor interface circuit, a wireless transceiver, the power management circuit and the flow control logic. Fabricated in $0.18{\mu}m$ standard CMOS technology, the SoC occupies a die area of ${\sim}9 mm^2$. The SoC consumes 6.15 mW from a 3 V power supply, guaranteeing the smart capsule battery life is no less than 24 hours when using 50 mAh coin batteries. The experimental results show that measurement accuracy of the smart capsule is ${\pm}0.1$ pH and ${\pm}0.2^{\circ}C$ for pH and temperature sensing, respectively, which meets the requirement of in-body pH and temperature monitoring in clinical practice.

Design and properties analysis of total internal reflection gratings for pulse compressor at 1053 nm

Qunyu Bi,Jiangjun Zheng,Ailin Guo,Meizhi Sun,Jianpeng Wang,Fuling Zhang,Qingwei Yang,Xinglong Xie,Zunqi Lin 한국물리학회 2011 Current Applied Physics Vol.11 No.1

High-efficiency compression gratings based on total internal reflection (TIR) are promising alternatives of compressor gratings because of their high diffraction efficiency, potential high damage resistant ability,and compact structure. Dependence of the 1 order diffraction efficiencies on grating parameters is analyzed for TE- and TM-polarized incident light of 1053 nm at Littrow angle, which is calculated by using the rigorous coupled-wave analysis. A more intuitional view on the relation is offered through three-dimensional slicing figures instead of two-dimensional ones. The performances of high-efficiency gratings are compared and regarded as criteria for further choices, including spectral bandwidth, angle bandwidth, dispersion, and intensity distribution. For TE- and TM-irradiations, similar spectral bandwidth and angle bandwidth can be achieved by different grating parameters. However, the computer simulation result on the intensity distributions of the two polarized waves shows that such design should be used under the illumination of TE-polarized wave for lower intensity enhancement ratio,which is an important factor related to the gratings’ damage threshold.