Canonical transient receptor potential channels and their modulators: biology, pharmacology and therapeutic potentials

Yuan-Yuan Gao,Wen Tian,Hui-Nan Zhang,Yang Sun,Jing-Ru Meng,Wei Cao,Xiao-Qiang Li 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.4

Canonical transient receptor potential channels(TRPCs) are nonselective, high calcium permeability cationicchannels. The TRPCs family includes TRPC1, TRPC2,TRPC3, TRPC4, TRPC5, TRPC6, and TRPC7. These channelsare widely expressed in the cardiovascular and nervoussystems and exist in many other human tissues and celltypes, playing several crucial roles in the human physiologicaland pathological processes. Hence, the emergenceof TRPCs modulators can help investigate these channels’applications in health and disease. It is worth noting that theTRPCs subfamilies have structural and functional similarities,which presents a signifi cant diffi culty in screening anddiscovering of TRPCs modulators. In the past few years,only a limited number of selective modulators of TRPCswere detected; thus, additional research on more potent andmore selective TRPCs modulators is needed. The presentreview focuses on the striking desired therapeutic eff ectsof TRPCs modulators, which provides intel on the structuralmodifi cation of TRPCs modulators and further pharmacologicalresearch. Importantly, TRPCs modulators cansignifi cantly facilitate future studies of TRPCs and TRPCsrelated diseases.

Endophytic Fungi from Dalbergia odorifera T. Chen Producing Naringenin Inhibit the Growth of Staphylococcus aureus by Interfering with Cell Membrane, DNA, and Protein

Yuan Gao,Yubin Ji,Wenlan Li,Fuling Wang,Fuling Wang,Xiaomeng Zhang,Zhihui Niu,Lulu Zhou,Lijun Yan 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.2

This study focused on the antibacterial effects of the endophytic fungi producing naringenin from Dalbergia odorifera T. Chen against Staphylococcus aureus. The antibacterial activity was measured by the inhibition diameters, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). The time-killing curve was also used to evaluate its antibacterial efficacy. The results of antibacterial activity determinations showed that endophytic fungi secondary metabolites can inhibit the growth of five pathogenic bacteria (S. aureus, Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, and Bacillus subtilis) and the most sensitive strain was S. aureus that had the MIC and MBC values of 0.13 and 0.50 mg/mL, respectively. The membrane permeability study was measured by a DNA leakage assay and electrical conductivity assay. Furthermore, the whole-cell protein lysates and DNA fragmentation assay was evaluated. The morphology of S. aureus treated with the endophytic fungi products was observed by scanning electron microscopy (SEM). The probable antibacterial mechanism of endophytic fungi secondary metabolites was the increased membrane permeability that leads to leaks of nucleic acids and proteins. SEM results further confirmed that the extracts can interfere with the integrity of S. aureus cell membrane and further inhibit the growth of bacteria, resulting in the death of bacteria. This study provides a new perspective for the antibacterial functions of endophytic fungi secondary metabolites for biomedical applications.

Physicochemical Characterization and an Injection Formulation Study of Water Insoluble ZCVI4-2, a Novel NO-Donor Anticancer Compound

Yuan Gao,Jianjun Zhang,Li Li,Feng Su,Zhenhua Gong,Yisheng Lai,Yihua Zhang 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.7

ZCVI4-2 was a novel nitric oxide-releasing glycosyl derivative of oleanolic acid that displayed strong cytotoxicity selectively against human hepatocellular carcinoma in vitro and in vivo. In this study, ZCVI4-2 was characterized by FT-IR spectroscopy, differential scanning calorimetry,powder X-ray diffractometry, Raman spectroscopy, hygroscopicity and stability. A high performance liquid chromatography method was also established for the quantitative determination of solubility and additional stability profile of ZCVI4-2. ZCVI4-2 was found to be an amorphous and stable solid with low solubility of less than 10 μg/mL. Based on the solubilization tests that included methods of cosolvency and micellization, the solution mixture of 5% Solutol HS-15, 5% 1, 2-propylene glycol and 5% anhydrous ethanol was determined to be the system for the preparation of the ZCVI4-2 early injection solution. The effect of pH, temperature, light and injectable isotonic glucose or NaCl solution on ZCVI4-2 injection was also investigated. Good stability was observed at all testing conditions. Under the conditions studied, the NO-releasing rate and amount of ZCVI4-2 from the early injection solution in rat plasma demonstrated a promising therapeutic efficacy while maintaining a good safety profile.

An Evaluation Method of Combat Aircraft Contribution Effectiveness Based on Mission Success Space Design

Yuan Gao,Hu Liu,Yaoming Zhou 한국항공우주학회 2019 International Journal of Aeronautical and Space Sc Vol.20 No.1

In the military field, equipment development has increasingly focused on the system-of-systems (SoS) oriented combat. To answer the question “how to judge the success of a mission in SoS combat”, this paper proposes a concept of “Mission Success Space (MSS)”. After designing MSS and computing Mission Success Rate (MSR), an evaluation method of effectiveness is defined to quantify the contribution of a combat aircraft to a combat SoS by comparing MSRs of the SoS with and without this aircraft. During MSS design procedure, Mission Success Function (MSF) plays an important role since it gives key parts where the success criterion is changed. In the simulation case, a quadratic function and an S-curve function are used as MSFs to verify themethod proposed. In addition, based on the Gaussian fitting results, a new function using inverse design is studied by finding bilateral quantiles of the fitting curves. Finally, the MSS of inverse design is chosen as the best for this case after analyzing the contribution results of these three functions.

Combined pre-detection and sleeping for energy-efficient spectrum sensing in cognitive radio networks

Gao, Yuan,Deng, Zhixiang,Choi, Dongmin,Choi, Chang Elsevier 2018 Journal of parallel and distributed computing Vol.114 No.-

<P><B>Abstract</B></P> <P>In this paper, we propose a cooperative spectrum sensing scheme including a combined pre-detection and sleeping policy. In the scheme, the designed pre-detection sub-phase is applied at the beginning of the detection of the presence of a primary user, where all sensing nodes are involved to improve the detection performance. The sleeping policy is applied for each sensing node respectively at the end of the pre-detection sub-phase and the beginning of the transmission of the local detection results, in order to decrease sensing energy consumption. We formulate the problem of minimizing the maximum average energy consumption per sensing node in Rayleigh fading channels, considering the constraints of the required global detection and false alarm probabilities and the tolerable interference caused to the primary user. Numerical results show that the scheme achieves significant energy saving as compared to a scheme that includes only a sleeping policy and does not consider pre-detection.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We propose a combined pre-detection and sleeping scheme for spectrum sensing. </LI> <LI> The sensing performance is not affected by a high sleeping rate of secondary relays. </LI> <LI> An analytical framework is based on more practical channel conditions. </LI> <LI> A search algorithm is studied to obtain low complexity for solving the optimal problem. </LI> <LI> The proposed scheme achieves significant energy saving for spectrum sensing. </LI> </UL> </P>

Spectrum Hole Utilization in Cognitive Two-way Relaying Networks

( Yuan Gao ),( Changping Zhu ),( Yibin Tang ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.3

This paper investigates the spectrum hole utilization of cooperative schemes for the two-way relaying model in order to improve the utilization efficiency of limited spectrum holes in cognitive radio networks with imperfect spectrum sensing. We propose two specific bidirectional secondary data transmission (BSDT) schemes with two-step and three-step two-way relaying models, i.e., two-BSDT and three-BSDT schemes, where the spectrum sensing and the secondary data transmission are jointly designed. In the proposed cooperative schemes, the best two-way relay channel between two secondary users is selected from a group of secondary users serving as cognitive relays and assists the bi-directional communication between the two secondary users without a direct link. The closed-form asymptotic expressions for outage probabilities of the two schemes are derived with a primary user protection constraint over Rayleigh fading channels. Based on the derived outage probabilities, the spectrum hole utilization is calculated to evaluate the percentage of spectrum holes used by the two secondary users for their successful information exchange without channel outage. Numerical results show that the spectrum hole utilization depends on the spectrum sensing overhead and the channel gain from a primary user to secondary users. Additionally, we compare the spectrum hole utilization of the two schemes as the varying of secondary signal to noise ratio, the number of cognitive relays, and symmetric and asymmetric channels.

Punicic Acid from Trichosanthes kirilowii Seed Oil Is Rapidly Metabolized to Conjugated Linoleic Acid in Rats

Gao-Feng Yuan,Jing-Qun Yuan,Duo Li 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.2

The incorporation and metabolism of orally administered punicic acid (PA), one isomer of conjugated linolenic acid (CLNA), in rat tissues and plasma were studied over a 24-hour period. The punic acid was derived from Trichosanthes kirilowii Maxim seed oil, a unique PA-containing material, and identified and analyzed by high-performance liquid chromatography and gas chromatography-mass spectrometry. The results show that PA was incorporated and metabolized to 9c,11t-conjugated linoleic acid (CLA) in rat plasma, liver, kidney, heart, brain, and adipose tissue. The level of PA and CLA in liver and plasma was higher than in brain, heart, kidney, and adipose tissue, and the lowest accumulation occurred in the brain. The observation that PA can be converted into 9c,11t-CLA has gained increased importance since it has been demonstrated that 9c,11t-CLA exerts many biological activities. Therefore natural resources containing CLNA, especially edible T. kirilowii seed, could be a potential dietary source of CLA, following PA metabolism. PA is expected to be used as a functional food and nutraceutical.

An Optimization Method for Designing LDPC Analog Decoders Based on Frequent Subgraph Mining Algorithm

Yuan Gao,Yujie Lin,Jibo Dai 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.10

To lower the mapping complexity of designing analog decoders, a method to optimize the design of low-density parity-check (LDPC) analog decoders is proposed in this paper. Based on factor graphs and the sum-product algorithm, the LDPC decoding process on the factor graph and the construction of analog decoders are exploited. Then the frequent subgraph mining algorithm is introduced to search the isomorphic subgraphs in factor graphs. According to the output of the frequent subgraph mining algorithm which enumerates all the subgraphs in factor graphs, the mapping complexity of a LDPC analog decoder can be significantly reduced. Finally, a (40, 16) LDPC analog decoder is constructed using the proposed method. Simulation results show that the need to place gates and connections can be reduced 90% and 23%, respectively, and the ideal performance is obtained by carefully choosing unit currents and decoding time.

Adaptive Cooperation for Bidirectional Communication in Cognitive Radio Networks

( Yuan Gao ),( Changping Zhu ),( Zhixiang Deng ),( Yibin Tang ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.3

In the interweave cognitive networks, the interference from the primary user degrades the performance of the cognitive user transmissions. In this paper, we propose an adaptive cooperation scheme in the interweave cognitive networks to improve the performance of the cognitive user transmissions. In the proposed scheme for the bidirectional communication of two end-source cognitive users, the bidirectional communication is completed through the non-relay direct transmission, the one-way relaying cooperation transmission, and the two-way relaying cooperation transmission depending on the limited feedback from the end-sources. For the performance analysis of the proposed scheme, we derive the outage probability and the finite-SNR diversity multiplexing tradeoff (f-DMT) in a closed form, considering the imperfect spectrum sensing, the interference from the primary user, and the power allocation between the relay and the end-sources. The results show that compared with the direct transmissions (DT), the pure one-way relaying transmissions (POWRT), and the pure two-way relaying transmissions (PTWRT), the proposed scheme has better outage performance. In terms of the f-DMT, the proposed scheme outperforms the full cooperation transmissions of the POWRT and PTWRT.

454 Pyrosequencing Analysis of Bacterial Diversity Revealed by a Comparative Study of Soils from Mining Subsidence and Reclamation Areas

( Yuan Yuan Li ),( Long Qian Chen ),( Hong Yu Wen ),( Tian Jian Zhou ),( Ting Zhang ),( Xia Li Gao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.3

Significant alteration in the microbial community can occur across reclamation areas suffering subsidence from mining. A reclamation site undergoing fertilization practices and an adjacent coal-excavated subsidence site (sites A and B, respectively) were examined to characterize the bacterial diversity using 454 high-throughput 16S rDNA sequencing. The dominant taxonomic groups in both the sites were Proteobacteria, Acidobacteria, Bacteroidetes, Betaproteobacteria, Actinobacteria, Gammaproteobacteria, Alphaproteobacteria, Deltaproteobacteria, Chloroflexi, and Firmicutes. However, the bacterial communities` abundance, diversity, and composition differed significantly between the sites. Site A presented higher bacterial diversity and more complex community structures than site B. The majority of sequences related to Proteobacteria, Gemmatimonadetes, Chloroflexi, Nitrospirae, Firmicutes, Betaproteobacteria, Deltaproteobacteria, and Anaerolineae were from site A; whereas those related to Actinobacteria, Planctomycetes, Bacteroidetes, Verrucomicrobia, Gammaproteobacteria, Nitriliruptoria, Alphaproteobacteria, and Phycisphaerae originated from site B. The distribution of some bacterial groups and subgroups in the two sites correlated with soil properties and vegetation due to reclamation practice. Site A exhibited enriched bacterial community, soil organic matter (SOM), and total nitrogen (TN), suggesting the presence of relatively diverse microorganisms. SOM and TN were important factors shaping the underlying microbial communities. Furthermore, the specific plant functional group (legumes) was also an important factor influencing soil microbial community composition. Thus, the effectiveness of 454 pyrosequencing in analyzing soil bacterial diversity was validated and an association between land ecological system restoration, mostly mediated by microbial communities, and an improvement in soil properties in coalmining reclamation areas was suggested.