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만성 견비통에 대한 동씨침 치료의 무작위 대조군의 임상 연구
김찬영,권나현,신예지,남동우,김건형,김종인,최도영,이윤호,이재동 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2007 東西醫學硏究所 論文集 Vol.2007 No.-
Objectives : To observe the effect of acupuncture treatment in chronic shoulder pain patients. Methods : 36 voluntary patients were randomly assigned to an acupuncture treatment group(E GrouP, n=18) and a control group(C GrouP, n=18). The E Group patients received acupuncture treatment on LI_(15), TE_(14), GB_(21) and Master Dong's acupuncture points, Shin-gwan and Gyun-joong, twice a week for four weeks. The C Group patients received no treatment. All patients in both groups were instructed to practise self exercise in their daily lives. Evaluations were made at baseline and after four weeks of study. The Constant Shoulder Assessment(CSA), Shoulder Pain and Disability Index(SPADI) and the patient's subjective pain was measured by Visual Analogue Scale(VAS). The obtained data was analyzed. Results '. The E Group showed significant(p<0.05) improvement in CSA, SPADI and VAS after four weeks of treatment. The C Group showed significant(p<0.05) improvement in CSA, but the change of SPADI and VAS was insignificant(P>0.05). CSA and SPADI of E Group significantly(p<0.05) improved compared to the C GrouP, but the difference of VAS change in the two groups was insignificant(p>0.05). Conclusions : Four weeks of acupuncture treatment significantly improved CSA, SPADI and VAS. The improvement of CSA and SPADI was significant(p<0.05) compared to untreated patients.
Ji-Hyeon PARK,Sung-Hyun JO,Jae-Seung LEE,Hyo-Jin JEON,Ji-Eun KWON,Ye-Rim KIM,Ji-Hyun BAEK,Min-Gyu KIM,Yun-Gon KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
The human gut commensal bacteria Faecalibacterium prausnitzii is well known for its anti-inflammatory effects that improve host intestinal health. Although several studies reported that inulin, one of the well-known prebiotics, increases the abundance of F. prausnitzii in the intestine, the mechanism under this effect of inulin remains unclear. Here, we applied LC-MS/MS-based multi-omics approaches to confirm the effects of inulin on F. prausnitzii. Interestingly, the proteomic analysis revealed that the putative proteins involved in sucrose utilization of F. prausnitzii are upregulated in the presence of inulin. To investigate the function of the proteins, we cloned the target genes, and observed the ability for sucrose degradation. In addition, we demonstrated that the sucrose degradation activity in F. prausnitzii culture media is enhanced by using inulin as a carbon source compared to glucose. Taken together, this study suggests that the reduction of sucrose induced by inulin could potentially improve host health and prevent gut dysbiosis like type 2 diabetes which is associated with sucrose.
Ji-eun KWON,Sung-Huyn JO,Jae-Seung LEE,Hyo-Jin JEON,Ji-Hyeon PARK,Ye-Rim KIM,Min-Gyu KIM,Ji-Hyun BAEK,Yun-Gon KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Clostridioides difficile is a gram-positive bacterium and is considered as an enteric pathogen. C. difficile colonizes the host with intestinal dysbiosis and produces virulence factors, Toxin A and B. Although the cytotoxicity of C. difficile has been well elucidated, the exact pathogenic mechanisms and biological pathways of C. difficile infection (CDI) are not yet clear. Here, we developed a CDI model using MIMICS to investigate C. difficile-host interactions throughout the early (12 hr) and later (48 hr) stages of infection. We also apply an LC-MS/MS-based multi-omics approach to study the physiological changes in CDI-induced gut epithelial cells at the molecular and cellular levels. The proteomic analysis confirmed that proteins involved in cellular responses such as stress, immune responses and DNA damage responses are upregulated at early stages of infection. In addition, a representative CDI phenotype has been identified in the late stage of the infection. Our in vitro CDI model and multi-omics approach not only provide a better understanding of host-anaerobic pathogen interactions but may also aid in the treatment of C. difficile and other anaerobic infections.
Protective effects of gomisin N against hepatic steatosis through AMPK activation
Yun, Ye-Rang,Kim, Ji-Hyun,Kim, Ji Ha,Jung, Myeong Ho Elsevier 2017 Biochemical and biophysical research communication Vol.482 No.4
<P><B>Abstract</B></P> <P>Gomisin N (GN) is a phytochemical derived from <I>Schisandra chinensis</I>. It has been reported to exert a protective effect against hepatic steatosis by attenuating endoplasmic reticulum (ER) stress. However, the detailed mechanism by which GN inhibits hepatic steatosis remains to be elucidated. In this study, we examined whether GN activates AMP-activated protein kinase (AMPK) and exerts therapeutic effects on liver X receptor (LXR)- or palmitic acid (PA)-induced triglyceride (TG) accumulation in HepG2 cells. Furthermore, <I>in vivo</I> protective effects of GN against hepatic steatosis were assessed in high-fat diet (HFD)-induced obese mice. GN stimulated the phosphorylation of AMPK, acetyl-CoA carboxylase (ACC), and sterol regulatory element-binding protein 1c (SREBP1c) in HepG2 cells. It decreased the expression of lipogenesis genes, but increased the expression of fatty acid oxidation genes. Additionally, GN decreased the expression of lipogenesis genes induced by the LXR agonist T0901317 or PA in HepG2 cells, resulting in reduced intracellular TG content. However, preincubation with compound C, an AMPK inhibitor, prevented GN-mediated effects. Administration of GN to HFD-induced obese mice decreased HFD-induced liver weight, hepatic TG accumulation, and cytoplasmic lipid droplet. These findings demonstrate that GN activates the AMPK pathway and ameliorates HFD-induced hepatic steatosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> GN stimulated the phosphorylation of AMPK, ACC and SREBP1c in HepG2 cells. </LI> <LI> GN downregulated lipogenesis genes and upregulated fatty acid oxidation genes. </LI> <LI> GN inhibited LXR or PA-induced lipogenesis and TG accumulation in HepG2 cells. </LI> <LI> GN inhibited hepatic steatosis in HFD obese mice. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Biological activity of Backhousia citriodora complex extract
Ji Hae Yun,Ye Ji Han,Mi Ran Kong 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Stress and lack of sleep cause reactive oxidant production in modern people, cuasing harmful effects such as decreased inmmunity. Therefore, antioxidant products capable of controlling oxidizing substances are in the spotlight. Through this study, we tried to confirm the possibility of use as a health functional food by 1:1:1(A) and 3:3:1(B) extracted ratios of Backhousia citriodora and Lyope platyphyla and Angelica gigas. Functional substances were mesured total polyphenol (TPC) and flavonoid content (TFC), and antioxidant activity was tested through DPPH, ABTS, SOD-like activity. As a result, A(TPC:71.41 mg/g, TFC: 16.3 mg/g) is showed higher functional material content than B(TPC: 33.46 mg/g, TFC: 6.55 mg/g). ABTS, DPPH radical scavenging also showed higher antioxidant activity in A(DPPH: 74.87% and ABTS: 44.19%) than B(DPPH: 46.77% and ABTS:33.38%) at 250 μg/mL concentrations. As a result of measuring the functional content and the ability to remove free radicals, the Backhousia citriodora complex extract was closely related to the antioxidant effect, The possibility of using the Backhousia citriodora composite as an antioxidant functional material was confirmed.
Biological activity of wine by-products by varieties of grapes
Ji Hae Yun,Ye Ji Han,Ho Jeong Bang,Dong Gyu Lee,Mi Ran Kong 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Grapes are produced in Korea amounted to about 175,000 tons at 2018. Wine by-products of grapes operated are simply consumed as feed or manure. In this study, we attempted to prove the possibility of grape by-products as antioxidants. Using three species of by-products of Vitis coignetiae (VC), Vitis Vinifera Muscat (VM), and Vitis Vinifera L (VL), we were used samples. According to result, total polyphenol (TPC), flavonoid (TFC), water-soluble protein (SPC), and reducing sugar (RS) we analyzed, they wer VC(TPC: 69.72 mg/g, TFC: 11.27 mg/g, SPC: 263.69 mg/g, RS: 263.86mg/g), VM(TPC:67.34 mg/g, TFC: 17.46 mg/g, WSP: 246.2 mg/g, RS: 159.15 mg/g), VL(TPC: 43.47 mg/g, TFC: 21.38 mg/g, WSP: 335.29 mg/g, RS: 106.86 mg/g). And antioxidant activity was tested through ABTS scavening activity and nitrite scavening ability (NSA). In order to VC, VM, and VL ABTS scavening activity was all higher than 97% at a concentration of 20 mg/mL. In addition, NSA at pH 1.2 showed high activity of 90%. Based on the above results, It is expected that grape by-products with high antioxidant effects and nitrite scavening ability can be processed and used as functional raw materials in the food industry.
Ji Ye Mok,Seung-Il Jeong,Jung-Keun Cho,Ji-Won Choi,Sang-Yun Nam,Won-Chil Chang,Byung-Eun Moon,Seon Il Jang 한국콘텐츠학회 2009 ICCC International Digital Design Invitation Exhib Vol.2009 No.12
Atopic dermatitis (AD) is a chronically relapsing prurtttc inflammatory skin disease. The effective microorganism fermentation extract (EM-X) is an antioxidant cocktail derived from the fermentation of plant material with effective microorganisms, it shows an anti-inflammatory, anticancer, and antioxidant effects. To find new anti¬inflammatory products for skin inflammatory disease such as AD and contact dermatitis, we produced the effective microorganism fermentation substance (EM-S) by fermentation of medicinal plants with effective microorganisms including photosynthetic bacteria, lactic acid bacteria and yeast, screened the effects of EM-S on mouse models of acute and chronic inflammatory responses. The effects of EM-S treatment inhibited 12-O-tetradecanoylphorbol 13-actate (TPA)-induced ear edema in Balb/c mice. Topically applied EM-S significantly reduced ear thickness, severity score and histological grade in AD-like NC/Nga mouse model by the house dust mite antigen (Dermatophagoides farinse extract) sensitization. In addition, the serum IgE and tissue Th2 chemokine levels were significantly reduced by EM-S. These results demonstrate that topical application of EM-S may be improve the AD-like skin lesion by suppressing IgE and Th2 chemokines.