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Carotenoids 식이와 알코올이 혈액학적 성분과 간조직에 미치는 영향에 관한 연구
조만희,김연선,이상한,우기민,장예진,김창세 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.2
Alcohol is well known agent which can damage the human tissues such as liver via stimulating lipid peroxidation and storage, denaturation of macromolecules, and inhibiting protein metabolism. On the other hand, carotenoids in addition to vitamins A, C, E and lipoic acid, play important roles in protecting these oxidative damages as well as preventing the production of free radicals. This study was carried out to elucidate the precise effects of alcohol administration into rats on the antioxidative functions of dietary carotenoids and isolated β-carotene, and to find out any parameters to uncover more detailed biochemical mechanisms of these agents. For these purposes, two different approaches were performed: 1) examination of the changes in hematological parameters (e.g., total proteins, A/G ratio, ALT/AST et. al) and statistical correlations among inter- and intragroups, 2) examination of the histopathological changes by an electron microscope. The results were analyzed and summarized as following; (1) Carrot diet for one week caused a slight increase in albumin. A/G ratio and AST levels, and a slight decrease in ALT, ALP, BUN and uric acid levels. Two-week alcohol administration following carrot diet increased total proteins, albumin A/G ratio and BUN levels. However, both groups were shown to have little significant changes in cholesterol concentrations. (2) Cheese diet for one week caused a significant increase in total proteins, albumin, AST, ALP, uric acid and total cholesterol levels, and a significant decrease in A/G ratio and glucose concentrations. However, two-week β-carotene diet following cheese intake increased total proteins albumin, A/G ratio, BUN and total cholesterol levels, whereas AST, ALT, ALP and uric acid levels were decreased. (3) Alcohol administration for one week caused an increase in AST activities and a decrease in total proteins, albumin, ALT, ALP and BUN levels. A successive β-carotene diet following alcohol administration increased total proteins, albumin, ALT, ALP and BUN levels, whereas decreased AST and uric acid levels. However, there were not significant changes in A/G ratio, glucose and total cholesterol levels in both groups. (4) β-carotene diet for one week caused a slight increase in albumin, glucose, AST and uric acid levels, and a slight decrease in ALP and BUN levels. Two-weeks alcohol administration following β-carotene diet increased albumin, glucose, BUN and total cholesterol levels, whereas decreased AST, ALT, ALP and uric acid levels. (5) Although the pathological investigation on the liver did not reveal significant changes, cheese diet group (CH-BC/1-CH) was shown to have some lipid deposits. Some results were unexpected and different from typical hematological changes shown by other researchers. Nonetheless, these results strongly suggest that the liver damage or hepatism caused by alcohol intake affects many kinds of biochemical metabolisms, which results in significant changes in many hematological parameters. In addition, dietary carotenoid and isolated β-carotene were shown to have protective roles against the biochemical changes by alcohol intake.
Study on the Environmental Risk Assessment of Transgenic Chinese Cabbage
Ye Sun Chung,Young Doo Park,Eun Taek Woo,Kuen Woo Park,Kyu Hwan Chung,Sang Yong Lee,Mun Il Ryoo,Jong Ok Ka,Min Jea Kim,Dong Ho Lee,Youn Hyung Lee,Dong Jin Lee,Chan Lee,Dae Yeul Son,Tae Sung Park,Hong 한국원예학회 2008 한국원예학회 기타간행물 Vol.- No.-
Vibrio crassostreae PKA 1002의 알긴산 분해 조효소 생산 최적 조건과 조효소의 특성
선우찬 ( Sun Woo Chan ),김꽃봉우리 ( Koth Bong Woo Ri Kim ),김동현 ( Dong Hyun Kim ),정슬아 ( Seul A Jung ),김현지 ( Hyun Jee Kim ),정다현 ( Da Hyun Jeong ),정희예 ( Hee Ye Jung ),임성미 ( Sung Mee Lim ),홍용기 ( Yong Ki Hong ),안 한국미생물생명공학회(구 한국산업미생물학회) 2012 한국미생물·생명공학회지 Vol.40 No.3
This study was conducted to screen an alginate-degrading microorganism and to investigate the characteristics of the alginate-degrading activity of its crude enzyme. Amarine bacterium which produces extracellular alginate-degrading enzymes was isolated from the brown alga Sargassum thunbergii. 16S rRNA sequence analysis and physiological profiling resulted in the bacterium`s identification as a Vibrio crassostreae strain, named Vibrio crassostreae PKA 1002. Its optimal culture conditions for growth were pH 9, 2% NaCl, 30℃ and a 24 hr incubation time. The optimal conditions for the alginate degrading ability of the crude enzyme produced by V. crassostreae PKA 1002 were pH 9, 30℃, a 48 hrincubation time and 8% alginic acid. The alginate degrading crude enzyme produced 3.035 g of reducing sugar per liter in 4% (w/v) alginate over 1 hr.
항MRSA (Methicillin Resistant Staphylococcus aureus) 활성을 나타내는 해양미생물의 분리
우예주 ( Ye Ju Woo ),도태웅 ( Tae Woong Do ),남예지 ( Ye Ji Nam ),안효정 ( Hyo Jung Ahn ),김미선 ( Mi -sun Kim ),전상아 ( Sang A Jeon ),정성윤 ( Seong Yun Jeong ) 대구가톨릭대학교 자연과학연구소 2011 자연과학연구논문집 Vol.9 No.1
Abstract:It was isolated marine bacterium, isolate YJ-1 which produced a bactericidal antibiotics against MRSA. This isolate was identified as tested by examining its morphological and biochemical properties, and 16S rDNA sequencing analysis for identification. This isolate YJ-1 was identified to the genus Pseudomonas. Therefore, this isolate was designated Pseudomonas sp. YJ-1. Pseudomonas sp. YJ-1. relatively grows well in the 25℃, pH 7.0, NaCl 1.0 %. For the purification of the bioactive compounds, YJ-1 was fermented in 37 L-PPES-Ⅱ medium, and culture filtrates of YJ-1 was extracted by ethyl acetate, hexane, and 80% MeOH. The 80% MeOH fraction of YJ-1 was showed the significant anti-MRSA (methicillin-resistant Staphylococcus aureus) activity against MRSA.
Short Communication : A Peptide Produced by Pseudomonas tolaasi, Tolaasin Binds to Metal Ions
( Sun Hee Lee ),( Geun Hyeong Jo ),( Do Seok Hwang ),( Yoon Kyung Woo ),( Young Giu Lee ),( Yeon Joong Yong ),( Kyung Rai Kang ),( Ji Ye Hyun ),( Young Kee Kim ),( Dong Woon Kim ),( Yoong Ho Lim ) 한국응용생명화학회(구 한국농화학회) 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Brown blotch disease in mushrooms is caused by Pseudomonas tolaasin, which produces a peptide toxin, tolaasin I, and zinc ion inhibits the channel formed by tolaasin I. NMR experiments revealed that zinc, sodium, and calcium ions can bind to tolaasin I and their binding position on tolaasin I is the lactone ring.
( Sun Young Ham ),( Ye Sol Bak ),( Tae Ho Kwon ),( Jeong Woo Kang ),( Kang Duk Choi ),( Tae Young Han ),( Il Young Han ),( Young Yang ),( Seung Hyun Jung ),( Do Young Yoon ) 한국응용생명화학회(구 한국농화학회) 2014 Journal of Applied Biological Chemistry (J. Appl. Vol.57 No.2
A1E is an extract from traditional Asian medicinal plants that has therapeutic activities against cancers, metabolic disease, and other intractable conditions. However, its mechanism of action on cervical cancer has not been studied. In order to ascertain if A1E would have pronounced anti-cervical cancer effect, cervical cancer cells were incubated with A1E and apoptosis was detected by nuclear morphological changes, annexin V-FITC/ PI staining, cell cycle analysis, western blotting, Reverse-transcription polymerase chain reaction, and measurement of mitochondrial membrane potential. Expression of human papiloma virus E6 and E7 oncogenes was down-regulated in A1E-treated cervical cancer cells, while p53 and retinoblastoma protein levels were enhanced. A1E also perturbed cell cycle progression at sub-G1 and altered cell cycle regulatory factors in SiHa cervical cancer cells. A1E activated apoptotic intrinsic pathway markers such as caspase-9, caspase-3 and poly ADP-ribose polymerase, and down-regulated expression of Bcl-2 and Bcl-xl. A1E induced mitochondrial membrane potential collapse and cytochrome c release, and inhibited phosphatidylinositol 3-kinase (PI3K)/Akt, key factors involved in cell survival signaling. Taken all these results, A1E induced apoptosis via activation of the intrinsic pathway and inhibition of the PI3K/Akt survival-signaling pathway in SiHa cervical cancer cells. In conclusion, A1E exerts anti-proliferative action growth inhibition on cervical cancer cells through apoptosis which demonstrates its anti-cervical cancer properties.