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이서래,김화영,최삼섭,승정자,오명숙,김동재 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-
In order to study the prevalence of constipation among female college students in Korea and the effect of yogurt, 3,243 students in Seoul area were surveyed by questionnaires for the status of stool evacuation and 197 students with possible constipation were sample and fed apple yogurt II from Pasteur Dairy Company for 3-weeks period. Students of constipation amounted to a high ratio of 53.4%, which consisted of 13.4% in heavy constipation(1-2 times evacuation per week)and 40.0% in mild constipation(3-4 times evacuation per week)during the past one-year period. To 90 of heavy constipation and 107 of mild constipation students were fed 290ml of yogurt every morning for 3-weeks period and examined for the status of stool evacuation by subsequent questionnaires. Frequency of stool evacuation was significantly improved in 51.3% of the students after 1 week, 57.9% after 2 weeks and 60.4% after 3 weeks(p<0.05). The effect of improvement in stool frequency was greater in heavy group than in mild group through the 3-weeks period. The number of students who experienced the disappearance of inconvenience in evacuation after drinking the yogurt was 52.3% after 1 week, 72.1% after 2 weeks and 76.1% after 3 weeks. Discomfort such as stomach disorder and mild diarrhea during the drinking period was observed in 24% students and it appeared that a longer drinking period was needed for adaptation of digestive tracts. The number of students maintaining the improved stool frequency was 38.7% after 1 week and 37.6% after 1 month since discontinuing yogurt drinking. The number of students reverted to the previous habit of evacuation after discontinuing was 51.6% after 1 week and 63.5% after 1 month, which were not significantly different between heavy and mild groups(p>0.05).
Bacillus cereus에 있어서 Phospholipase C활량에 관한 몇가지 계면활성제의 영향
권오용,윤희주,한수자 충남대학교 자연과학연구소 1987 忠南科學硏究誌 Vol.14 No.2
This study demonstrates the effects of four detergents and three on the activity change of phospholipase C which is prepared from the cultural broth of Bacillus cereus. This assay of phospholipase C activity was carried out with phosphatidylcholine micelles which were mixed with the detergents and sterols, with increase a molar ratio of these compounds to phosphatidylcholine. Phospholipase C was highly activated at 0.5 molar ratio of Tween 80-mixed micelles. In the case of Triton X-100, it was activated at higher molar ratio indicated 1.0. Sodium dodecyl sulfate was more effective than that of the other detergents at lower molar ratio. However sodium deoxycholate-mixed micelles increased the activity more 7 times than that of the control group. This maximum activation was occurred at 2.5 molar ratio and the turbidity was gradually decreased. Cholesterol and ergosterol increased the activity until molar ratio reached up to 0.9. But the activity was higher at 0.3 molar ratio for stigmasterol. Consequently, it was indicated that phospholipase C was activated with the supplement of detergents until the mixed micelles made a number of small and spherical shape.