Tumor Necrosis Factor-α-Activated Human Adipose Tissue–Derived Mesenchymal Stem Cells Accelerate Cutaneous Wound Healing through Paracrine Mechanisms

Heo, Soon Chul,Jeon, Eun Su,Lee, Il Hwan,Kim, Hoon Soo,Kim, Moon Bum,Kim, Jae Ho The Society for Investigative Dermatology, Inc 2011 The Journal of investigative dermatology Vol.131 No.7

Human adipose tissue–derived mesenchymal stem cells (ASCs) stimulate regeneration of injured tissues by secretion of various cytokines and chemokines. Wound healing is mediated by multiple steps including inflammation, epithelialization, neoangiogenesis, and proliferation. To explore the paracrine functions of ASCs on regeneration of injured tissues, cells were treated with tumor necrosis factor-α (TNF-α), a key inflammatory cytokine, and the effects of TNF-α-conditioned medium (CM) on tissue regeneration were determined using a rat excisional wound model. We demonstrated that TNF-α CM accelerated wound closure, angiogenesis, proliferation, and infiltration of immune cells into the cutaneous wound in vivo. To assess the role of proinflammatory cytokines IL-6 and IL-8, which are included in TNF-α CM, IL-6 and IL-8 were depleted from TNF-α CM using immunoprecipitation. Depletion of IL-6 or IL-8 largely attenuated TNF-α CM-stimulated wound closure, angiogenesis, proliferation, and infiltration of immune cells. These results suggest that TNF-α-activated ASCs accelerate cutaneous wound healing through paracrine mechanisms involving IL-6 and IL-8.

Impaired Memory in OT-II Transgenic Mice Is Associated with Decreased Adult Hippocampal Neurogenesis Possibly Induced by Alteration in Th2 Cytokine Levels

Minho Moon,Seong Gak Jeon,Kyoung Ah Kim,Hyunju Chung,Junghyun Choi,Eun Ji Song,Seung-Yun Han,Myung Sook Oh,Jong-Hwan Park,Jin-il Kim 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.8

Recently, an increasing number of studies have focused on the effects of CD4+ T cell on cognitive function. How-ever, the changes of Th2 cytokines in restricted CD4+ T cell receptor (TCR) repertoire model and their effects on the adult hippocampal neurogenesis and memory are not fully understood. Here, we investigated whether and how the mice with restricted CD4+ repertoire TCR exhibit learning and memory impairment by using OT-II mice. OT-II mice showed decreased adult neurogenesis in hippocampus and short- and long- term memory impairment. Moreover, Th2 cytokines in OT-II mice are significantly increased in peripheral organs and IL-4 is significantly increased in brain. Finally, IL-4 treatment significantly inhibited the proliferation of cultured adult rat hippocampal neural stem cells. Taken together, abnormal level of Th2 cytokines can lead memory dysfunction via impaired adult neurogenesis in OT-II transgenic.

Comparison of inflammatory markers for the prediction of neointimal hyperplasia after drug-eluting stent implantation

Kang, Woong Chol,Il Moon, Chan,Lee, Kyounghoon,Han, Seung Hwan,Suh, Soon Yong,Moon, Jeonggeun,Shin, Mi Seung,Ahn, Taehoon,Shin, Eak Kyun Lippincott Williams Wilkins, Inc. 2011 Coronary artery disease Vol.22 No.8

BACKGROUND: We compared the relationship between inflammatory markers and neointimal hyperplasia (NIH) after drug-eluting stent (DES) implantation. METHODS: We implanted a single DES in 42 consecutive patients with stable angina. The plasma high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), and matrix metalloproteinase-9 (MMP-9) levels were measured before, and 24 and 72 h after the procedure. Angiography and intravascular ultrasound were performed. RESULTS: No relationship was noted between the baseline hs-CRP level and NIH. A significant positive correlation was noted between NIH and the hs-CRP level obtained at 24 h (r=0.435, P=0.004), and 72 h (r=0.334, P=0.031) after the procedure. Interestingly, there was a positive correlation between the change (&Dgr;) in the hs-CRP level and NIH at 24 h (r=0.414, P=0.006). The fourth quartile of the hs-CRP at 24 h after percutaneous coronary intervention (PCI) had significantly larger volume of NIH than the first quartile (20.1±25.1 vs. 2.7±6.4 mm, P<0.05). Moreover, NIH in the fourth quartile (20.9±26.4 mm) was higher than the first quartile (3.3±8.6 mm) of the &Dgr; hs-CRP level at 24 h (P<0.05) after the procedure. Although the IL-6 level at the baseline and 72 h after the procedure were positively correlated with NIH (r=0.337, P=0.029 and r=0.435, P=0.004, respectively), the &Dgr; IL-6 level at any stage was not correlated with NIH. Neither the MMP-9 level nor the &Dgr; MMP-9 level at any stage was correlated with NIH. CONCLUSION: This prospective intravascular ultrasound study showed the inflammatory response after PCI, as measured by hs-CRP levels, but not the baseline hs-CRP level, predict NIH after DES implantation. Neither a change in the IL-6 nor MMP-9 levels at any stage after PCI reflected NIH.

배출수 처리공정의 진단

문용택,최일환 대한상하수도학회 2002 상하수도학회지 Vol.16 No.6

結晶의 32個 三次元的 點群의 誘導

徐日煥,金光柱,徐種命,李珍昊,林星秀,金文執 충남대학교 1990 忠南科學硏究誌 Vol.17 No.1

結晶들의 外的인 모양은 매우 規則的이며 많은 對稱性을보인다. 結晶들이 나타내는 外的인 對稱要素들은 10가지로 分類되는데 이 對稱要素들은 모든 結晶들을 7個의 系로 分類한다. 이 對稱要素들이 한 固定點올 지나도록 모을 수 있는 個別의 集合의 數는 32個뿐으로 이를 三次元的 點群이라 한다. 이 論題에서는 32個 點群을 誘導하고 整理하였다. Crystals are very regular in external shape and clearly exhibit a great deal of symmetry. The external symmetry elements exhibited by crystals are categorized into a limited number of ten and they classify all crystals into the seven crystal systems. The total number of distinctly different sets of the symmetry elements passing through a fixed point comes out to be only thirty two which are termed as the three-dimensional point groups. The thirty two point groups are derived and summarized here.

7個 結晶系의 Patterson 函數

徐日煥,金光柱,李珍昊,金文執 충남대학교 1990 忠南科學硏究誌 Vol.17 No.2

적은數의 重原子를 갖은 分子의 結晶構造는 重原子法(heavy-atom method 또는 Patterson method 라고도 함)으로 解析될수 있다. 本 論題에서는 重原子法에 利用되는 -般的인 Patterson 函數 및 7個 結晶系名名의 Patterson 를 誘導하였으며 이 函數를 使用하여 決定된 三個의 結晶構造를 實例로 들었다. Crystal structures of certain molecules containing a relatively small number of heavier stoms could be analyzed by the Patterson method which is alternatively called heavy-atom method. In this topic, the general Patterson function and the one for each of the seven crystal systems used for the heavy-atom method were derived and three crystal structures determined by these functions were illustrated as examples.

X-線 廻折方法에 依한 Sodium Chloride 單結晶의 構造 硏究

金文執,尹和重,徐日煥 충남대학교 자연과학연구소 1981 忠南科學硏究誌 Vol.8 No.1

The procedure for the crystal structure analysis by means of the X-ray powder diffraction method has been shown using the sodium chloride as a sample. The twelve X-ray diffraction intensity peaks for sodium chloride powder were collected rotating 2θhkl. Bragg angle of X-ray diffractometer from 20 to 160 degrees, in which Cu Kα radiation filtered by nickel had been used. For assigning indices of each reflecting plane, the relationship between lattice parameter and interplanar spacing has been used. This result has shown that sodium chloride is face-centered cubic, space group ?? with unit cell dimension a=5.63Å and z=4. The experimental and theoretical integrated intensities of the reflecting planes are in good agreement with each other.

ASYMUNT: 21개 Noncentric 점군이 갖는 X선 데이터의 asymmetric Units

서일환,박정란,박영수,이진호,이창희,김문집 순천향대학교 기초과학연구소 1999 순천향자연과학연구 논문집 Vol.5 No.2

결정학적 가설 ??은 anomalous dispersion을 나타내는 원자를 갖고있는 비대칭 결정에 대해서는 성립하지 않는다. 만약 이들의 결정구조가 Lane group 보다는 오히려 noncentric point group의 X선 강도 데이터의 asymmetric unit로부터 정해질 수 있다면, 그 구조는 강도 데이터가 2배로 증가하기 때문에 보다 신뢰성이 증가한다. 본 논문에서는, 21개 noncentric point group 각각에 들어가는 강도 데이터의 asymmetric unit 의 정확한 범위를 계산하는 프로그램을 제안하였다.

X-線粉末 廻折法에 의한 Cadmium Sulfide의 精密 格子常數 測定

金文執,徐日煥 순천향대학교 1983 논문집 Vol.6 No.3

In our previous paper the crystallographic structure of cadmium sulfide had been determined. In the present paper we calculated the precise lattice parameter of them. We gave the method of extrapolation a trial for CdS having hexagonal and cubic structures. The final values calculated from the method are A=6.723128 Å, B=4.148240 Å for hexagonal CdS, and A=5.8604 Å for cubic. The correct result has been proved by the good agreement with other reported values.

구강악안면 영역의 치성 감염 환자에 대한 세균학적 연구

김일규,윤승환,오성섭,최진호,오남식,김의성,이성호,배수환,강문수 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.4

Oral & maxillofacial infections are most commonly odontogenic in origin. Although such infections are usually self-limiting, they may occasionally spread deeply into fascial spaces or planes far from the initial site of involvement. If early diagnosis and appropriate therapy is delayed, complications such as mediastinal extension, retropharyngeal spread and airway obstruction could happen to the patients. For the study of the microbiology, we have retrospectively analysed the oral & maxillofacial infected patients in the Dept. of Oral & Maxillofacial Surgery, In-Ha University Hospital from 1997 September to 2000 April. The results were as follows 1.The male patients were more common than female, with male 61.9% and female 38.1%. 2.Dental originated infections were most common cause with the incidence of 62%. 3.Most common fascial space involved was buccal space 42cases(37.2%) followed by submandibular space 13cases(11.5%), infraorbital space 13cases(11.5%), masseteric space 11cases(9.7%) , periapical abscess 11cases(9.7%) . 4.The causative organisms isolated from the pus culture were Gram Positive Bacterial species, which were 46cases(31.9%) of Streptococcus viridans, 16cases(8.6%) of α and β-hemolytic streptococcus, 4cases(3.1%) of Strep.-group D non enterococci, 7cases(5.1%) of Staphylococcus Coa. Neg., 5cases(3.9%) of Staphylococcus aureus, 3cases(2.3%) of Enterococcus faecalis, 1case(0.8%) of Bacillus species, 1case(0.8%) of Peptostreptococcus, 1case(0.8%) of Clostridium and Gram negative bacterial species, which were 4cases(3.1%) of Acinetobacter baumannii, 2cases(1.6%) of Pseudomonas aeruginosa, 2cases(1.6%) of Burkholderia cepacia, 1case(0.8%) of Neisseria species, 1case(0.8%) of Klebsiella pneumoniae, 1case(0.8%) of Klebsiella oxytoca, 1case(0.8%) of Escherichia coli. 5.In drug sensitivity test, high resistant tendency was found in Penicillin system(Penicillin G 83.3%, Ampicillin 60%) and Aminoglycosides (Gentamycin 50%, Tobramycin 45.5%), but tertiary Cephalosporin system(Cefoperazone 9.1%, Ceftazidime 18.2%), and glycopeptides system (Teicoplanin 0% , Vancomycin 0%) showed lower resistancy.