Nephrin phosphorylation regulates podocyte adhesion through the PINCH-1-ILK-α-parvin complex

( Dong Qing Zha ),( Cheng Chen ),( Cheng Chen ),( Xing Hua Chen ),( Tean Ma ),( Hong Xia Yang ),( Harry Van Goor ),( Guo Hua Ding ) 생화학분자생물학회 2013 BMB Reports Vol.46 No.4

Nephrin, a structural molecule, is also a signaling molecule after phosphorylation. Inhibition of nephrin phosphorylation is correlated with podocyte injury. The PINCH-1-ILK-?-parvin (PIP) complex plays a crucial role in cell adhesion and cytoskeleton formation. We hypothesized that nephrin phosphorylation influenced cytoskeleton and cell adhesion in podocytes by regulating the PIP complex. The nephrin phosphorylation, PIP complex formation, and F-actin in Wistar rats intraperitoneally injected with puromycin aminonucleoside were gradually decreased but increased with time, coinciding with the recovery from glomerular/podocyte injury and proteinuria. In cultured podocytes, PIP complex knockdown resulted in cytoskeleton reorganization and decreased cell adhesion and spreading. Nephrin and its phosphorylation were unaffected after PIP complex knockdown. Furthermore, inhibition of nephrin phosphorylation suppressed PIP complex expression, disorganized podocyte cytoskeleton, and decreased cell adhesion and spreading. These findings indicate that alterations in nephrin phosphorylation disorganize podocyte cytoskeleton and decrease cell adhesion through a PIP complex-dependent mechanism.

Molecular Orbital Gating Surface-Enhanced Raman Scattering

Guo, Chenyang,Chen, Xing,Ding, Song-Yuan,Mayer, Dirk,Wang, Qingling,Zhao, Zhikai,Ni, Lifa,Liu, Haitao,Lee, Takhee,Xu, Bingqian,Xiang, Dong American Chemical Society 2018 ACS NANO Vol.12 No.11

<P>One of the promising approaches to meet the urgent demand for further device miniaturization is to create functional devices using single molecules. Although various single-molecule electronic devices have been demonstrated recently, single-molecule optical devices which use external stimulations to control the optical response of a single molecule have rarely been reported. Here, we propose and demonstrate a field-effect Raman scattering (FERS) device with a single molecule, an optical counterpart to field-effect transistors (a key component of modern electronics). With our devices, the gap size between electrodes can be precisely adjusted at subangstrom accuracy to form single molecular junctions as well as to reach the maximum performance of Raman scattering via plasmonic enhancement. Based on this maximum performance, we demonstrated that the intensity of Raman scattering can be further enhanced by an additional ∼40% if the orbitals of the molecules bridged two electrodes were shifted by a gating voltage. This finding not only provides a method to increase the sensitivity of Raman scattering beyond the limit of plasmonic enhancement, but also makes it feasible to realize addressable functional FERS devices with a gate electrode array.</P> [FIG OMISSION]</BR>

Helicobacter pylori Chaperone-Like Protein CagT Plays an Essential Role in the Translocation of CagA into Host Cells

( Ding Hong Lei ),( Hao Zeng ),( Lin Ping Huang ),( Yan Dong Dong ),( Yi Jun Duan ),( Xu Hu Mao ),( Gang Guo ),( Quan Ming Zou ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.10

Most of the Helicobacter pylori strains containing the cag pathogenicity island (PAI) have been associated with more severe gastric disease in infected humans. The cag PAI is composed of 27 proteins, and some of the components are required for CagA translocation into host cells as well as induction of proinflammatory cytokines, such as interleukin-8 (IL-8); however, the exact function of most of the components remains unknown or poorly characterized. In this study, we demonstrated that CagT (HP0532), which is an essential structural component of the cag PAI apparatus, plays an important role in the translocation of CagA into host epithelial cells. In addition to being located on the bacterial surface, CagT is also partially localized in the inner membrane, where it acts as a chaperone-like protein and promotes CagA translocation. However, CagT secretion was not detected by immunoprecipitation analysis of cell culture supernatants. Meanwhile, CagT was related to the introduction of IL-8 of the host cell. These results suggest that CagT is expressed on both the inner and outer bacterial membranes, where it serves as a unique type IV secretion system component that is involved in CagA secretion and cag PAI apparatus assembly.

Ubiquitination of p53 is Involved in Troglitazone Induced Apoptosis in Cervical Cancer Cells

Chen, Hui-Min,Zhang, Ding-Guo,Wu, Jin-Xiz,Pei, Dong-Sheng,Zheng, Jun-Nian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Peroxisome proliferator-activated receptor gamma (PPAR-${\gamma}$), a ligand-dependent nuclear transcription factor, has been found to widely exist in tumor tissues and plays an important role in affecting tumor cell growth. In this study, we investigated the effect of PPAR-${\gamma}$ on aspects of the cervical cancer malignant phenotype, such as cell proliferation and apoptosis. Cell growth assay, Western blotting, Annexin V and flow cytometry analysis consistently showed that treatment with troglitazone (TGZ, a PPAR-${\gamma}$ agonist) led to dose-dependent inhibition of cervical cancer cell growth through apoptosis, whereas T0070907 (another PPAR-${\gamma}$ antagonist) had no effect on Hela cell proliferation and apoptosis. Furthermore, we also detected the protein expression of p53, p21 and Mdm2 to explain the underlying mechanism of PPAR-${\gamma}$ on cellular apoptosis. Our work, finally, demonstrated the existence of the TGZ-PPAR-${\gamma}$-p53 signaling pathway to be a critical regulator of cell apoptosis. These results suggested that PPAR-${\gamma}$ may be a potential therapeutic target for cervical cancer.

Cordblood-Based High-Throughput Screening for Deafness Gene of 646 Newborns in Jinan Area of China

Shou-Xia Li,Ding-Li Chen,Su-Bin Zhao,Li-Li Guo,Hai-Qin Feng,Xiao-Fang Zhang,Li-Li Ping,Zhi-Ming Yang,Cai-Xia Sun,Gen-Dong Yao 대한이비인후과학회 2015 Clinical and Experimental Otorhinolaryngology Vol.8 No.3

Objectives. Infants with slight/mild or late-onset hearing impairment might be missed in universal newborn hearing screening (UNHS). We identified the mutation hot spot of common deaf gene in the newborns in Jinan area population by screening the mutation spot with neonate cord blood, in order to make clear whether the neonate cord blood for screening is feasible. Methods. Six hundred and forty-six newborns were subjected to both UNHS and genetic screening for deafness by using neonate cord blood. The newborn genetic screening targeted four deafness-associated genes, which were commonly found in the Chinese population including gap junction beta-2 protein (GJB2), gap junction beta-3 protein (GJB3), solute carrier family 26 member 4 (SLC26A4), and mtDNA 12S rRNA. The most common 20 spot mutations in 4 deaf genes were detected by MassARRAY iPLEX platform and mitochondrial 12S rRNA A1555G and C1494T mutations were sequenced using Sanger sequencing. Results. Among the 646 newborns, 635 cases passed the UNHS and the other 11 cases (1.7%) did not. Of the 11 failures, two cases were found to carry homozygous GJB2 p.R143W pathogenic mutation, one case was found to have heterozygous GJB2 235delC mutation, and another one case carried heterozygous GJB3 p.R180X pathogenic mutation. Six hundred and thirty-five babies passed the newborn hearing screening, in which 25 babies were identified to carry pathogenic mutations, including 12 heterozygotes (1.9%) for GJB2 235delC, eight heterozygotes (1.3%) for SLC26A4 IVS7-2A>G, one heterozygote (0.2%) for p.R409H, two homozygotes (0.3%) for m.1494C>T, and two homozygotes (0.3%) for m.1555A>G. Conclusion. Newborn genetic screening through the umbilical cord blood for common deafness-associated mutations may identify carriers sensitive to aminoglycoside antibiotic, and can effectively prevent or delay hearing loss occurs.

Morphological and Molecular Phylogenetic Data of the Chinese Medicinal Fungus Cordyceps liangshanensis Reveal Its New Systematic Position in the Family Ophiocordycipitaceae

( Yao Wang ),( Yong-dong Dai ),( Zhong-lin Yang ),( Rui Guo ),( Yuan-bing Wang ),( Zhu L. Yang ),( Lei Ding ),( Hong Yu ) 한국균학회 2021 Mycobiology Vol.49 No.4

A cordycipitoid fungus infecting Hepialidae sp. in Nepal was supposed to be identical to Cordyceps liangshanensis, originally described from southwestern China, and thus, transferred to the genus Metacordyceps or Papiliomyces in previous studies. However, our multi-gene (nrSSU-nrLSU-tef-1a-rpb1-rpb2) phylogenetic and morphological studies based on the type specimen and additional collections of C. liangshanensis revealed that the fungus belongs to the genus Ophiocordyceps (Ophiocordycipitaceae). Therefore, a new combination O. liangshanensis was made, and a detailed description of this species was provided.

硏究論文 : 중국 사막지역의 방풍책 높이와 공극률에 따른 방풍효과 및 설치비용 비교분석

박기형 ( Ki Hyung Park ),( Guo Dong Ding ),( Guang Ling Fang ),김찬범 ( Chan Beom Kim ),( Bin Wu ),( Yan Feng Bao ),( Guang Lei Gao ),정성철 ( Sung Cheol Jung ),문강민 ( Kang Min Moon ) 한국환경복원기술학회(구 한국환경복원녹화기술학회) 2012 한국환경복원기술학회지 Vol.15 No.6

This study was conducted in Ningxia Hui autonomous region, located at southern part of Mu Us sand land in China. To investigate relationships between windbreak effect and installation cost of sand barriers, plastic net is utilized by using four kind of heights (0.2, 0.3, 0.4 and 0.5m) and four kind of porosities (20, 30, 50 and 70%). These heights and porosities are measured for estimating distances for effective windbreak. It is shown that porosity and the distance have a positive relationship at same heights and porosity on ground indicates a constant figure when height reaches a certain level, regardless of the porosity. This implies that there is a difference of level of windbreak with different porosities; however, distance of windbreak effect is same at the same height of sand barrier. As a result of comparison between porosity of sand barrier on the ground and installation cost in each sand barrier with various heights and porosities (16 combinations), 0.4m and 0.5m height sand barriers describe highest economical efficiency. Within two variables, we concluded that height has a higher impact on windbreak effect than porosity.

Analysis of the Interference Effects in CMOS Image Sensors Caused by Strong Electromagnetic Pulses

Yang Zhikang,Wen Lin,Li Yudong,Zhou Dong,Wang Xin,Ding Rui,Zhong Meiqing,Meng Cui,Fang Wenxiao,Guo Qi 한국전자파학회 2024 Journal of Electromagnetic Engineering and Science Vol.24 No.2

With the electromagnetic environment becoming increasingly complex, it is crucial to address the risk posed by electromagnetic pulse, which critically impairs the performance and reliability of electronic systems based on complementary metal oxide semiconductor (CMOS) image sensors. In this context, research on the failure types of CMOS image sensors in a high-power electromagnetic environment, caused by strong electromagnetic pulses and the rapid evaluation method of interference immunity, has garnered significant interest. This paper conducts electromagnetic pulse simulation experiments on CMOS image sensors to first study their failure types, such as image abnormalities and functional interruption, and then identify the corresponding failure criteria. Furthermore, this study builds on the small sample test evaluation method to investigate the interference threshold of functional interruptions in CMOS image sensors by calculating the failure probability at different field strengths. The obtained data were combined with the Weibull distribution function for fitting, the results of which found the interference threshold to be at 40.4 kV/m. The findings of this study provide a basis for evaluating the survivability of CMOS image sensors and their associated reinforcement technology in high-power electromagnetic environments.

대도시의 소규모 생물서식공간(Biotop)조성과 관리 방안

박율진 ( Yool Jin Park ),정국동 ( Guo Dong Ding ) 한국녹지환경디자인학회 2008 녹지환경학회지 Vol.4 No.3

In the treatise this author suggested measures for creation and management of small scale Biotop in megalopolis. Basic direction of plan for creating Biotop was researched through available literature. Actual problems related to work execution and management were analysed and rational measures at the time of design and ex post facto management were presented. Contents of research include status of ecological ponds, pond for dragonflies, ponds for frogs, ecological streams, its types and classification and design standard. Result of searching for measures for creating and managing Biotop based on study of above mentioned status and problems is as follows: When record of carrying out the projects for 4 years by Seoul City is reviewed it could be found that the scale of the project was about 89,700m2 of the target area of 7 parks inclusive of Namsan Park (around Chunil Mineral Water Spring) and ecological ponds, erection of observation decks and planting dietary plants. The required budget was about 1,814 million won. It is annually engaged in creating a city for symbiosis of man and organism through creating environment where variety of animals and plants can live together by creating small scale Biotop possible for improving and development of diversity of organism.

Broadband Absorption Enhancement in µc-Si:H Thin-Film Solar Cells Based on Silver Nanoparticle Arrays

Shi-E Yang,Ping Liu,Dong Ding,Qiaoneng Guo,Yong-Sheng Chen 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.3

The thin-film solar cell structure with a broadband absorption enhancement is reported. We designed spherical silver (Ag) nanoparticle arrays on or embedded partially into indium tin oxide (ITO) layer of the hydrogenated microcrystalline silicon (µc-Si:H) thin-film solar cells. The geometrical parameters, such as nanoparticle radius (R), array period (P) and ITO layer thickness (d) are optimized by using the finite element method (FEM). The numerical results show that the key parameter that influences the integrated absorption is period/radius ratio (P/R) for Ag nanoparticle arrays. Embedding nanoparticle arrays partially into ITO layer with the appropriate thickness can improve broadband light-trapping. The optimized structure shows 50.1% enhancement in the integrated absorption compared to the reference cell when P = 500 nm, R = 100 nm and d = 70 nm. Furthermore, physical mechanisms of absorption enhancement in different wavelength range are discussed according to the electrical field amplitude distributions in the solar cells.