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Measurements of charge exchange neutral particles in Heliotron J
masashi Kaneko,F. Sano,H. Okada,K. Hanatani,K. Nagasaki,K. Kondo,S. Kobayashi,S. Murakami,T. Mizuuchi,Y. Suzuki,Y. Nakamura 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.III
The ion behavior in Heliotron J plasmas is investigated through measurements of the ion energy distribution function, which is measured with a charge-exchange neutral particle analyzer system. The bulk ion temperature is estimated from the measured energy spectrum. In NBI heated plasma, the pitch angle distribution of energy spectra is measured. Dependence of the high energy tail and bulk ion temperature of injection power is investigated in an ion-cyclotron range of frequency heating experiment.)
Masashi Emoto,Masakazu Kaneko,Yoshiko Emoto 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.2
Macrophages (Mo|) play a pivotal role in the protection system by recognizing and eliminating invading pathogenic bacteria. Phagocytosis and the killing of invading bacteria are major effector functions of Mo|. Although the phagocytic and bactericidal activitiesof Mo| have been analyzed via several methods using a light microscope, a fluorescence microscope, or a fluorescence-activatedcell sorter, expensive materials and equipment are usually required, and the methods are rather complicated. Moreover, it is impossible to determine both the phagocytic and bactericidal activities of Mo| simultaneously using these methods. In this review,we describe a simple, reproducible, inexpensive, yet old-fashioned method (antibiotic protection assay) for determining the phagocytic and bactericidal activities of Mø.
Novel fabrication technologies of planar nano-gap electrodesfor single molecule evaluation
Ken Tsutsui,Masashi Nakata,Masayuki Morita,Masahide Tokuda,Kazuyuki Nagatsuma,Harumasa Onozato,Tadao Kaneko,Tomohiko Edura,Yoshio Mita,고이누마히데오미,Yasuo Wada 한국물리학회 2007 Current Applied Physics Vol.7 No.4
Present information technologies use semiconductor devices and magnetic/optical disc. However, they are all foreseen to face funda-mental limitations within a decade. Therefore, superseding devices are required for the next paradigm of high performance informationare expected as the most probable candidate to supersede the present semiconductor devices. The rst milestone towards the realizationof single molecule devices in future electronics requires quantitative evaluation of single molecule characteristics, which inevitably needsplanar nano-gap electrodes between which single molecules are sandwiched, observed their structures and measured their electrical char-acteristics. Nano-meter electrode pattern fabrication was achieved by electron beam lithography and metal lift-o, while planarizationprocessing technologies are described in this paper to realize nm-planar nano-scale electrodes.
최혁재,Shingo Kaneko,Masashi Yokogawa,송관필,김대신,강신호,Yoshihisa Suyama,Yuji Isagi 한국식물학회 2013 Journal of Plant Biology Vol.56 No.4
The conservation status of Euchresta japonica Hook. f. ex Regel in Korea was investigated, with an emphasis on its genetic diversity. From field surveys, we obtained the only locality record for a wild population in Korea, which contained eight individuals. Genotyping was performed using nine microsatellite markers for all 20 remaining individuals, including those in ex situ collections. Among nine microsatellite loci that amplified within this group, five showed polymorphism with low hererozygosities, and a total of 12 multilocus genotypes were detected. Wild-specific alleles were detected in two individuals, and ex situ-specific alleles were detected in six individuals. Five individuals proved to have individual-specific alleles. The Korean population was also distinguished from the previously reported Japanese population by different alleles and higher diversity. To conserve this species more effectively in Korea, we recommend the following: (1) fencing the remaining wild population; (2) no relocation of wild individuals, as nine ex situ plants are already available; (3) complete ex situ conservation of all genetic diversity via clonal propagation of wild individuals; and (4) continuous protection and monitoring of the wild population.
Ensiled Green Tea Waste as Partial Replacement for Soybean Meal and Alfalfa Hay in Lactating Cows
Kondo, Makoto,Nakano, Masashi,Kaneko, Akemi,Agata, Hirobumi,Kita, Kazumi,Yokota, Hiroomi Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.7
The purpose of this study was to evaluate the effects of protein supplementation of green tea waste (GTW) on the performance of lactating cows. Another aim was to increase resource utilization and to eliminate any environmental negative impact from the tea waste. GTW from a beverage company was ensiled at a low pH (<4.0) and high acetic acid and lactic acid concentration, and it contained high crude protein (CP, 34.8%), total extractable tannins (TET, 9.2%) and condensed tannin (CT, 1.7%). Two experiments were conducted to investigate the palatability and performance in lactating cows fed GTW. In the palatability trial, three lactating cows were allocated to three dietary treatments in a 3$\times$3 Latin square design. The animals were offered a total mixed ration (TMR) including GTW at rates of 0, 2.5 and 5.0% on a dry matter (DM) basis. Total DM intake was not different among the treatments. In the performance trial, four lactating cows were used in a 2$\times$2 Latin square design with a 3 week sampling period. GTW was incorporated into TMR at a rate of 5.0% on a DM and 10.0% on a CP basis. Thus GTW replaced alfalfa hay and soybean meal at a level of 25.0% on a DM. DM and CP intake were not affected by the inclusion of GTW, whereas TET and CT intake were significantly increased (p<0.001). Milk production, milk composition and the efficiency of milk production were not altered by the GTW inclusion. Although ruminal pH and VFA, and blood urea nitrogen were not changed, ruminal $NH_{3}-N$ and plasma total cholesterol were relatively low in the GTW group, but not significantly different. The excretion of urinary purine derivatives and estimated MN supply were also not significantly affected by GTW treatment. It is therefore concluded that GTW can be used as a protein source without any detrimental effects on the performance of lactating cows.
Takuya Ito,Shingo Kaneko,Masashi Yokogawa,송관필,최혁재,Yuji Isagi 한국식물분류학회 2013 식물 분류학회지 Vol.43 No.1
Hydrangea luteovenosa is a critically endangered plant species of Jeju Island in Korea, though it is widely distributed in western Japan. We isolated and characterized five microsatellite loci in this species. The number of alleles ranged from 3 to 27, observed heterozygosity from 0.27 to 0.86, and expected heterozygosity from 0.34 to 0.91. The markers described here will be useful for investigating the genetic diversity, genetic structure, and gene flow of H. luteovenosa, and the genetic findings would contribute to the establishment of effective conservation measures for this species in Korea.
Choi, Hyeok-Jae,Ito, Takuya,Yokogawa, Masashi,Kaneko, Shingo,Suyama, Yoshihisa,Isagi, Yuji The Korean Society of Plant Taxonomists 2017 식물 분류학회지 Vol.47 No.1
The population status of Hydrangea luteovenosa Koidz. in Korea was investigated, with an emphasis on its genetic diversity. From field surveys, we obtained the only locality record for a wild population in Jeju Island, which contained 285 individuals in total. Genotyping was performed using five microsatellite markers for the all extant plants in Korea. Three Japanese populations were also genotyped for the comparative analyses. The genotyping result showed that the Jeju population consisted of only two multilocus genotypes, including identical heterozygous genotypes at two loci; it had been maintained mostly by vegetative reproduction; and although the Jeju population is geographically far from Japanese populations, all alleles observed in the Korean population were shared with Japanese populations, suggesting the possibility that H. luteovenosa in the Jeju Island had been recently migrated or introduced from Japan. Future ecological and genetic studies associated with negative effects of low genetic variation will be essential for determining the conservation direction of the threatened Korean population of this species.