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최혁재,Shingo Kaneko,Masashi Yokogawa,송관필,김대신,강신호,Yoshihisa Suyama,Yuji Isagi 한국식물학회 2013 Journal of Plant Biology Vol.56 No.4
The conservation status of Euchresta japonica Hook. f. ex Regel in Korea was investigated, with an emphasis on its genetic diversity. From field surveys, we obtained the only locality record for a wild population in Korea, which contained eight individuals. Genotyping was performed using nine microsatellite markers for all 20 remaining individuals, including those in ex situ collections. Among nine microsatellite loci that amplified within this group, five showed polymorphism with low hererozygosities, and a total of 12 multilocus genotypes were detected. Wild-specific alleles were detected in two individuals, and ex situ-specific alleles were detected in six individuals. Five individuals proved to have individual-specific alleles. The Korean population was also distinguished from the previously reported Japanese population by different alleles and higher diversity. To conserve this species more effectively in Korea, we recommend the following: (1) fencing the remaining wild population; (2) no relocation of wild individuals, as nine ex situ plants are already available; (3) complete ex situ conservation of all genetic diversity via clonal propagation of wild individuals; and (4) continuous protection and monitoring of the wild population.
Takuya Ito,Shingo Kaneko,Masashi Yokogawa,송관필,최혁재,Yuji Isagi 한국식물분류학회 2013 식물 분류학회지 Vol.43 No.1
Hydrangea luteovenosa is a critically endangered plant species of Jeju Island in Korea, though it is widely distributed in western Japan. We isolated and characterized five microsatellite loci in this species. The number of alleles ranged from 3 to 27, observed heterozygosity from 0.27 to 0.86, and expected heterozygosity from 0.34 to 0.91. The markers described here will be useful for investigating the genetic diversity, genetic structure, and gene flow of H. luteovenosa, and the genetic findings would contribute to the establishment of effective conservation measures for this species in Korea.
Hajime Ohira,Koichi Sato,Tadaaki Tsutsumi,Shingo Kaneko,최혁재 국립중앙과학관 2018 Journal of Asia-Pacific Biodiversity Vol.11 No.3
Pseudoscorpions are one of the common arthropod in soil mesofauna but are infrequently studied in East Asia. The fauna in South Korea is not adequate enough, and practical faunistic survey of pseudoscorpions have not been conducted in last 20 years. In this article, the current pseudoscorpion fauna in South Korea is reviewed, and the results of the survey in Chungcheongnam-do Province, the DNA barcoding (mitochondrial DNA cytochrome c oxidase I sequencing) and molecular phylogenetic analysis are shown. A total 64 specimens, including four species, Allochthonius (Allochthonius) buanensis, Bisetocreagris japonica, Bisetocreagris turkestanica (first record from South Korea), and Microbisium pygmaeum, were collected. The result of molecular phylogenetic analysis based on the cytochrome c oxidase I sequences (427 bp) implied the existence of a possible cryptic species within A. (A.) buanensis. Faunistic survey and DNA barcoding of South Korean species can greatly contribute to the understanding of East Asian pseudoscorpion systematics.
Choi, Hyeok-Jae,Ito, Takuya,Yokogawa, Masashi,Kaneko, Shingo,Suyama, Yoshihisa,Isagi, Yuji The Korean Society of Plant Taxonomists 2017 식물 분류학회지 Vol.47 No.1
The population status of Hydrangea luteovenosa Koidz. in Korea was investigated, with an emphasis on its genetic diversity. From field surveys, we obtained the only locality record for a wild population in Jeju Island, which contained 285 individuals in total. Genotyping was performed using five microsatellite markers for the all extant plants in Korea. Three Japanese populations were also genotyped for the comparative analyses. The genotyping result showed that the Jeju population consisted of only two multilocus genotypes, including identical heterozygous genotypes at two loci; it had been maintained mostly by vegetative reproduction; and although the Jeju population is geographically far from Japanese populations, all alleles observed in the Korean population were shared with Japanese populations, suggesting the possibility that H. luteovenosa in the Jeju Island had been recently migrated or introduced from Japan. Future ecological and genetic studies associated with negative effects of low genetic variation will be essential for determining the conservation direction of the threatened Korean population of this species.