Detection of Puumala and Hantaan Viruses among Bats in Korea by Nested RT-PCR

Lee, Yun-Tai,Yun, Bo-Kyoung,Yoon, Jeong-Joong The Korean Society of Virology 1998 Journal of Bacteriology and Virology Vol.28 No.2

Hantavirus is a genus of the Bunyaviridae family consisting following serotype groups: Hantaan, Seoul, Puumala, Prospect Hill, Thailand, Belgrade, Thotta palayam, Sin Nombre. Most of Hantavirus group have been associated with many clinically similar disease known collectively as hemorrhagic fever with renal syndrome (HFRS). Hantaan virus is the prototype of the genus hantavirus, originally isolated from Apodemus agrarius. Bat was found as a natural host for Hantaan virus in Lee's lab for the first time. Then, Hantaan-like virus was isolated Hantaan-like virus from bat. To identify hantaviruses that are present in Korea among bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area, RNA was isolated from lung and serum. RT-PCR was performed with a universal primer from M segment. Nested RT-PCR was carried out to differentiate Hantaan, Seoul and Puumala virus using serotype specific primers. As we expected, Hantaan viruses were detected in bats and Seoul virus was not detected. Interestingly, Puumala viruses were also detected in bats from Won-Ju, but not in other areas. Puumala virus is originally isolated from Clethrinomys glareolus, and cause light HFRS. Recently, Paradoxomis webbiana, a wild bird turn out to be a reservoir for Puumala virus in Korea. These data indicate that bat is a new natural reservoir of Puumala virus.

Detection of Puumala and Hantaan Virus among Bats in Korea by Nested RT - PCR

Yun Tai Lee,Bo Kyoung Yun,Jeong Joong Yoon 대한바이러스학회 1998 Journal of Bacteriology and Virology Vol.28 No.2

Hantavirus is a genus of the Bunyaviridae family consisting following serotype groups: Hantaan, Seoul, Puumala, Prospect Hill, Thailand, Belgrade, Thotta palayam, Sin Nombre. Most of Hantavirus group have been associated with many clinically similar disease known collectively as hemorrhagic fever with renal syndrome (HFRS). Hantaan virus is the prototype of the genus hantavirus, originally isolated from Apodemus agrarius. Bat was found as a natural host for Hantaan virus in Lee's lab for the first time. Then, Hantaan-like virus was isolated Hantaan-like virus from bat. To identify hantaviruses that are present in Korea among bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area, RNA was isolated from lung and serum. RT-PCR was performed with a universal primer from M segment. Nested RT- PCR was carried out to differentiate Hantaan, Seoul and Puumala virus using serotype specific primers. As we expected, Hantaan viruses were detected in bats and Seoul virus was not detected. Interestingly, Puumala viruses were also detected in bats from Won-Ju, but not in other areas. Puumala virus is originally isolated from Clethrinomys glareolus, and cause light HFRS. Recently, Paradoxomis webbiana, a wild bird turn out to be a reservoir for Puumala virus in Korea. These data indicate that bat is a new natural reservoir of Puumala virus.

韓國에서 分離한 Leptospira의 細菌學的 特性에 관하여

李淵台,張大一 단국대학교 1988 論文集 Vol.22 No.-

Bacteriological dharacters of strains that kindly furnished from the division of pathogenic bacteria, NIH, school, were performed. All the strains were isolated from the wild-rat of Yea Ju (Kyong-Gi), Won ju (Knag-won) Kwang San, Ccheon-Nam) from Sseptember to November, 1985 and stock cultured in that laborlatory, The results were as follow; 1. Fifteen Leptospira interrogans was isolated from 50 wild-rat collexted in Yeoju, and Kwangsan areas. 2. According to tha rodent species, the isolation rate was different; the isolation rate from Apodemus agarius was 32.6% and the rate from Rattus novegicus was 16.7%. 3. Leptospira isolates had motility, photoplasmic spiral, axial filament and enveloping membrane, and hook in the one end or both end of the cell. The size of the cell was 0.1㎛ in diameter and 6∼12㎛ in length, and sixteen to twenty times coiled along the axial filament of the cell. 4. The growth rate was best in the media in which 8 to 10% rabbit serum was contained under 29℃ culture condition. The cell was inhibited to the growth at the addition of 225㎛ /ml of 8-azaguanine in the media. 5. The isolates was shown as peroxidase positive, cat alase positive and oxidase positive. 6. The microcopical histo-pathological sign from the infected guinea pig with Leptospira was diffused pale-redness spot in the lung, pale-whiteness in the kidney. 7. Microscopical histo-pathological sign was shown as follows; The swelling of the epihelial cell in tubule urethra with vaccuole or ulcer was shown in the kidney, and hydropic dgeneration in the interstitial tissue after the duration of 5 days from the guinea pig inoculated with Leptospira. Congestion and haemorrhagic sign was most characteristic in the lung after the duration of 5 days from the guinea pig incoculated with Leptospira. Epithelial cell in bronchi was increased and red cells and degenerated epithelial cells was also observed. 8. The LD_50 value inoculated by intra peritoneal route into guinea pig 8.9×10 exp (4) of the cells.

人體에서 分離한 葡萄糖 非醱酵菌의 抗菌劑 耐性에 關한 硏究

李淵台,崔承求,朴哲熙,曺圭鳳 단국대학교 대학원 1991 學術論叢 Vol.15 No.-

This experiment was conducted to classify for the 82 strains of glucose nonfermentative bacteria obtained from K hospital, in 1988, on the biological differentials and to test the resistance reaction on the drugs for those strains. The results were as follows : 1. Eighty two strains were classified into 65 strains of 79.3% of Ps. aeruginosa, 10 strains of 12.2% of Ps. cepacia, and 7 strains of 8.5% of Ac. calcoaceticus as results of 0 F sugar test, oxidase test, and DNase test. 2. It showed that Ps. aeruginosa was coincided with AP, CM, GM, KM, CF, and TC from the disk and MIC methods in the test of resistance reaction to antimicrobial agent, and especially, that AK and TOB KM and GM were observed high resistance reactive from MIC method. Resistnace to antibiotics for Ps. cepacia showed the identical reaction to AP, CB, CM, AK, SPT, CF, TC, TOB, KM and GM were observed high resistance reative from MIC method. Ac. calcoaceticus was identified the same resistance reaction to antibiotics to CB, KM, GM, AK, CF, and TOB, AP, SPT and TC showed high resistance reaction from the disk method. 3. CF and AP were observed the highest resistance reactions among 10 kinds of drugs used in this experiment and following was CM, KM and TC in order. 4. Ps. aeruginosa, Ps. cepacia, and Ac. calcoaeticus showed all multiple resistance reactions in the test of resistance to drugs, and Ps. aeruginosa was identified high resistance reaction to 6 drugs in 24.6%, Ps. cepacia was 9 drugs in 60%, and Ac. calcoaceticus showed resistance reaction to various kinds of drugs. 5. It showed important question in result of these studies that resistance percentage of NFB decreased by optimal antimicrobial selection and effective treatment established from serious antimicrobial treatment.

大腸菌의 痢疾菌의傳達性 R-plasmid 에 關한 硏究

李淵台,박경란 대한감염학회 1983 감염 Vol.15 No.1

One hundred and twenty-five strains of Escherichia coli isolated from either stools of Human or drainage were tested for their resistance to antimicrobial drugs and their R-plasmid transfer. 1. The frequency of resistance strains were highest to tetracycline(Tc) with 60%, and followed by ampicillin(Ap), streptomycin(Sm), kanamycin(Km) in the decreasing order, ranging from 57.6% to 18.4% and only five strains were resistant either one or more antibiotics of gentamicin(Gm), rifampicin(Rf), and amikacin(Ak). The frequency of resistant strains were much higher among human isolates than drainage one. There was difference in drug resistance between human and drainage isolated. 2. Seventy-two of isolated from human strains 94% were resistant to one or more drugs tested, and fifty-three strains isolated from drainage strains fourty-seven were resistant to one or more drugs tested. However strains resistant to four or more drugs were much more appear among human isolates than drainage isolated, with the increasing tendancy of mutiple resistant drugs among human isolates, following the increase in the number of resistant drugs. 3. In the transfer test of drug resistance by conjugation of sixty-eigth strains which isolated from human fourty-three strains(63.2%) were transferable, whereas of twenty-eight strains isolated from drainage eight strains(32%) were transferable. 4. A total of 117 strains of Shigella isolated in Korea from 1982 to 1983 were tested for gruds resistance and distribution of R-plasmid. Most strains were shown multiples drug resistance and transfer experiments of multiple drug resistance to E. coli ML 1410 showed that all the strains resistant to three or more drugs carried R-plasmids.

장티프스환자의 다형핵 백혈구에 대한 Chemotaxis

李淵台,李鍾訓 大韓免疫學會 1979 大韓免疫學會誌 Vol.1 No.1

In this study, an attempt was made to measure the chemotatic activity of PMNs in Salmonellosis. Each 15 ml of heparinized blood was obtained from 6 patients of typhoid fever in the Northwick Park Hospital and Clinical Research Centre from June in 1978 to Novemer. Each 1 ml of 3% dextran was added and the treated blood was incubated at 37°C for 4C minutes. The supernatant was harvested, washed two times with TC-199 medium, and the concentration of cells was adjusted at I X 10' ml. The casein (0.5 %) was used as a chemotactic factor with Boyden chamber modified by Larson (1977) used. The pores of the membrane millipore filters were 0.8pm and 0, 45pm in diameter (filter type SC made in France). The chamber inserted filters was filled with 0.4 ml of PMNs and kept at 37°C for 2. +hrs. Then, picked out the filter, we stained it with Harris haematoxicillin and counted only five fields through the microscope. The results were as follows: 1. The chemotactic activity of PMNs in control had no significant differences. 2. The longer the measuring time was, the more migrated the cells were. But, in this study a chamber was activated for 2. + hrs. 3. In typhoid fever, the chemotactic activity of PMNs appeared with a remarkable difference between one early infected stage and the other convalescent. Namely, the reactivity of the early infected stage was lower than that of the convalescent one.

1975년도 사람 및 가금(닭) 혈청내에 일본뇌염바이러스의 항체보유율

李淵台,李鍾訓 대한감염학회 1976 감염 Vol.8 No.1

Japanese encephalitis had been one of the most serious endemic disease during summer time from 1949 to 1968 in Korea, but since 1969 it has markedly been decreased by the unknown factors. However, Japanese encephalitis has still occurred in some areas and it was much concerned to the public as a endemic disease every year. For these reasons, the present investigation was performed by using hemagglutination inhibition (HI) test (Clarke & Casals, 1958). The total of 594 of human sera from the out-patients of St. Mary's hospital were tested and 1,008 fowl sera were collected in four different areas of Korea from July to November, 1975, respectively. The following results were obtained. 1. The total 56.4% of the 594 cases were confirmed as a HI antibody and 58.3% in 278 male cases out of the 594 and 54.8% of 316 cases the rate of female. 2. In the group whose age was from 41 to 50, it was 71.2% and this shows that it was markedly higher than others. 3. Seasonal distribution of HI antibody was shown to be 63.1% in 306 cases in September, 1975 but in other season it was to have a low. 4. The total 8.3% of the 1,008 fowl sera were confirmed to have an antibody on the four areas. 5. Geographical distribution of antibody in the four fowl areas were different from each other, and Seoul areas is the highest as 9.8% in 634 cases. 6. Seasonal distribution of HI antibody in the fowls were shown to be 16.4% in 165 cases in August.

冷血動物(개구리)에서 日本腦炎 바이러스의 抗體보유에 관한 硏究

이연태,기영진,김광현,최성학,홍장선 단국대학교 신소재기술연구소 1993 신소재 Vol.3 No.-

일본뇌염 바이러스는 극동지방에서 여름철에 Culex tritaeniorhynchus 홍모기를 매개로 사람과 가축에 감염되여 질병을 유발하고 치명적인 결과를 초래하는 무서운 바이러스 질환의 원인체이다. 그동안 끊임없는 연구결과로 1970년 후반부터 일본뇌염 환자발생이 현저하게 감소하였다. 그러나 자연계에서 일본뇌염 바이러스의 생태학적 규명은 아직도 연구되어야 할 과제들이 산적되어 있다. 따라서 이 바이러스의 보유동물에 대한 자연계의 생활환경에 관한 연구가 필요하여 개구리의 항체보유여부를 규명 하였다. 6개도의 야외에서 채집한 개구리 681마리 혈청에 대하여 HI 방법에 의한 항체 분석을 시행하여 다음과 같은 결과를 얻었다. 1. 개구리 총 681마리의 일본뇌염바이러스에 대한 HI항체 보유율은 9.83%였다(p<0.01). 2. 일본뇌염바이러스에 대한 성별 HI 항체 보유율은 수컷이 2.63%(531마리 중 14마리). 암컷이 35.33%(150마리 중 53마리)로 암컷이 약 13배 높은 항체보유율을 나타냈다(p<0.01). 3. 종류별 HI항체 보유율은 북방산 개구리가 100%(1마리 중 1마리), 산개구리 66.66%(3마리 중 2마리), 금개구리 50.00%(36마리 중 18마리), 옴개구리 33.33%(6마리 중 2마리), 참개구리 6.92%(635마리 중 44마리)로 나타났다(p<0.01). 4. 월별 HI 항체 보유율은 11월이 66.66%(9마리 중 6마리)로 가장 높았으며 10월이 37.50%(32마리 중 6마리), 9월이 26.05%*142마리 중 37마리), 8월이 22.85%(35마리 중 8마리), 9월이 26.05%(142마리 중 37마리), 8월이 22.85%(35마리 중 8마리), 6월이 1.65%(241마리 중 4마리), 5월은 0%(222마리 중 0마리)이였다(p<0.01). 5. 지역별 일본뇌염바이러스에 대한 HI 항체보유율은 경기 16.56%(157마리 중 26마리, 충남 15.69%(172마리 중 27마리), 전북 7.14%(168마리 중 12마리)였고, 충북(71마리)과 강원(78마리)은 0%였다(p<0.010. Japanese Encephalitis virus(JEV) infects human and domestic animals via mosquitoes, Culex tritaeniorhynchus, and cause diseases that results in serious consequencies in Far East area during summer season. Thanks to the continuous research on this virus, the frequency of occurrence has been greatly diminished since the second half of 1970. But, there are still much work needs to be done on the virus, especially on their ecology in nature. We studied the antibodies in frogs based on the perspective that the research must be done on the carrier animals of the virus. We tested sera by HI method from 681 frogs captured randomyl in the six provinces. The results are summarized as following 1. Antibody positive rate to HI was 9.83%(67 out of 681). 2. Among these positive frogs, positive rate for male was 2.63%(14 out of 531), and positive rate for famale was 35.33%(53 out of 150). the positive rate for female is higher than that of male by about 13 times. 3. Different positive rates were shown among different species; 100%(1 out of 1) in Rana temporaria ornativentris, 66.66%(2 out of 3) in Rana temporaria orativentris, 50%(18 out of 36) in Rana plancyi chosenica, 33.33%(2 out of 6) in Rana rugosa and 6.92%(44 out of 635) in Rana niglomaculata. 4. When the monthly positive rates were compared, November was the highest as 66.66%(6 out of 9), 37.5%(6 out of 32) in October 26.05%(37 out of 142) in September, 22.85%(8 out of 35) in August, 65%(4 out of 241) in June and 0% in May 5. Areal distribution of positive rate to HI was 16.56%(26 out 157) in Kyung Kee, 15.69%(27 out of 172) in Chung Nam, 7.14%(12 out of 168) in Chun Buk, and 0% both in Chung Buk and Gang Won.

의과대학생의 자연최면경험과 최면유도점수의 상관관계

이정태,최윤정,전양환,성양숙,박원명,김광수,유태열 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.5

목 적 : 저자들은 자연최면경험과 최면감수성간의 관계를 알아보기 위하여 자연최면점수와 최면유도 척도로 얻은 최면유도점수 및 유도점수와의 관계를 알아보고, MBTI(Myers-Briggs Type Inventory)의 성격유형과 이들의 상호관련성을 알아보려고 하였다. 방 법 : 가톨릭대학교 의과대학에 재학중인 63명의 의대생들을 대상으로 하여 자연최면경험에 대한 설문지와 MBTI를 작성하게 하고 최면유도척도로 최면을 유도하면서 최면유도점수와 유도점수를 얻었다. 결 과 : 1) 자연최면점수와 유도점수 사이에서 양의 상관관계가 있었고, 최면유도점수와 유도점수 사이에도 양의 상관관계가 있었다. 2) 자연최면점수와 최면유도점수 사이에서는 통계적으로 유의한 상관관계가 없었다. 3) MBTI의 각 성격유형들은 최면유도점수와 유도점수 사이에서 양의 상관관계가 있는 것으로 나타났다. 4) 외향형과 사고형의 성격유형은 자연최면점수와 최면유도점수에서 각각 양의 상관관계가 있었고, 자연최면점수와 유도점수 사이에서도 양의 상관관계가 있었다. 5) 직관형과 판단형은 자연최면점수와 유도점수 사이에서 각각 양의 상관관계를 보였다. 결 론 : 자연최면점수는 유도점수와 상관관계가 있었다. MBTI의 외향형과 사고형에서는 자연최면경험이 많을수록 최면유도가 잘 된다는 것을 시사하였다. Objective : We attempted to investigate the relation between naturally occurring hypnotic experiences and hypnotizability. We examined the correlations among hypnotic induction profile scores, natural hypnotic scores, induction scores and MBTI personality types. Methods : Sixty-three medical students completed NHQ(natural hypnotic questionnaire) developed from a list of naturally occuring hypnotic-like experiences and MBTI(Myers-Briggs Type Inventory). HIP(Hypnotic Induction Profile) was also administered to all the subjects. HIP score and IND(induction score) were obtained. Results : 1) The NHS(natural hypnotic score) was significantly correlated with IND in all the subjects. The IND also showed significant correlation with HIP score. 2) There was no significant correlation between NHS and HIP scores. 3) HIP scores were positively correlated with induction scores in all types of MBTI. 4) Natural hypnotic scores were positively correlated with HIP scores and induction scores in introvert and thinking types. 5) There were positive correlations between natural hypnotic scores and induction scores in intuition and judgment types. Conclusion : The natural hypnotic scores were correlated with induction scores. It is suggested that the more the naturally-occurring hypnotic experience is experienced, the better the hypnotic induction is induced in introvert and thinking types of MBTI personality types.

補益藥의 補血機轉에 對한 本草學的 解釋

이제현,이한구,김진숙,김연태,김정숙 대한본초학회 1997 大韓本草學會誌 Vol.12 No.2

Hematopoietic actions induced by administration of Astragali radix, Angelicae gigantis radix, Rehmanniae radix preparat and Cervi pantotrichum cornu were compared in SAM R1 and P6. We measured complete blood cells such as RBC, Hb and Hct using Coulter's method and compared the results each other in a view of traditional Korean medicine. The administration of Angelicae gigantis radix did not induce hernatopoietic actions in SAMs and also was quite different those from the other 3 herbs. Thus, the mechanisms of the hematopoietic action by Astragali radix, Angelicae gigantis radix, Rehmanniae radix preparat and Cervi pantotrichum cornu could be different each other.