유색미에 관한 연구 : Ⅲ. 유색미 유망계통에 대한 주요 생육 특성

김광수,최윤표,김선택,최현구,정종태,김보경,유지홍,이희봉 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

국내외 유색미 수집종을 돌연변이원에 의해 유기된 우수 계통과 수집종간 상호교배에 의해 선발된 CNU 20계통을 파종하여 얻어진 주요 작물학적 특성을 살펴보면 다음과 같다. 1. 간장은 유색 메벼인 CNU126 계통이 대조품종인 동진벼와 같이 가장 컸고 메성인 CNU 128계통에서 가장 작았다. 2. 주당 분얼수는 CNU3, CNU50. CNU56, CNU112 계통에서 가장 많았고 대조품종과 CNU 128 계통에서 10개 미만으로 가장 적게 나타났다. 3. 수장은 육성 계통 중 CNU88 계통에서 가장 길었으나 대조구보다 짧고, 육성계통 중에서는 CNU 126 계통이 가장 짧았다. 4. 주당 영화수는 대조구인 동진벼보다 CNU50 계통에서 2배 이상 많았고, CNU158 계통에서 가장 적게 나타났다. 5. 천립중은 CNU113 계통에서 30g이상으로 대조구와 같이 가장 무거웠고, CNU128 계통에서 20g으로 가장 적었다. 6. 주당 수량은 대조구의 26.6g에 비해 CNU50, CNU112 계통에서 두배정도 높았고, CNU128, CNU158, CNU200 계통에서 가장 낮게 타나났다. Aims of this study were carried out to develop the useful lines induced from mutation and pedigree breeding methods among the collected genetic resources from national and domestic areas. In this study, Stem height of CNU126 line and check among them were high, while CNU128 was lower than other lines. Spiklet length of CNU88 was longer, but that of CNU126 was shorter than check. Number of spiklets per plant of CNU50 among lines have twice time than check. 1,000 grains weight of CNU113 was higher than check. In yield per plant, CNU50 and CNU112 were higher than check, Dongjinbyeo.

동일한 시료에 대한 국내 기관간의 STR 분석결과 비교 : STR 유전자좌 분석법의 표준화 설정을 위하여

박종태,신경진,양윤석,우광만,이숭덕,이승환,이정빈,정연보,조승희,한길로,한면수,홍승범 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

This paper described a collaborative exercise intended to see what kinds of short tandem repeat (STR) loci are used in different DNA typing laboratories in Korea and to compare their results for the demonstration whether uniformity of DNA profiling results from different laboratory could be achieved in Korea Laboratories were asked to test five tissue DNAs using methods routinely used in each laboratory and to report the results to the coordinating laboratory. The exercise demonstrated that each laboratory was using different STR loci for the typing with different STR numbers,2 VNTRS,36 STRs and amelogenin in total, and the direct comparison of the results from all the laboratory for the 18 loci could not be done as only one laboratory submitted typing results. Among 21 loci for which several laboratories submitted typing results, results for 14 loci were the same and results for the other 7 loci were different depending on the participating laboratory. D1S80, F13A01, D16S539, D21S11, D18S51, D3S1744 were the loci with different typing results. Even in the cases where commercial kits were used, the results were not the same depending on the machines used, that is the capillary electrophoresis or the gel based electrophoresis. The reason for the different results, points about the standardization of the methods arid the profiling data were described.

창원지역에서의 대기오염물질 배출량 산정

김유근,박상철,문윤섭,오인보,황미경 부산대학교 환경문제연구소 2002 環境硏究報 Vol.20 No.-

Emissions of air pollutants (TSP, SO2, NO2, CO, and HC) was estimated in Changwon city that has the industrial complex and basin terrain. We found that the emissions of TSP, SO2, NO2, CO, and HC were 897 tons, 3,287 tons, 5,706 tons, 10,057 tons, and 1,470 tons for a year, respectively and the main source of air pollutants were vehicle. The ratios of vehicle emissions to total emissions ware TSP 63.8%, SO2 26.1%, NO2 65.5%, CO 91.0%, HC 94.4%. The ratios of industry emissions to total emissions were TSP 30.8%, SO2 60.2%, NO2 25.2%, CO 6.4%, HC 3.7%. The ratio of life emissions to total emissions are TSP 5.4%, SO2 13.7%, NO2 9.3%, CO 2.6%, HC 1.9%. The concentration distributions of air pollutants simulated by ISCST3 model were similar to its emission distributions, and relatively high concentrations of air pollutants occurred in the Palyong-dong (industrial area) and Banlim and Chungang (residual area).

신고리 지역 기상관측을 통한 계절별 대기안정도 특성분석

송상근,김유근,오인보,정주희,조윤미,우경은,강태훈 부산대학교 환경문제연구소 2003 環境硏究報 Vol.21 No.-

In this study, we analyzed the characteristics of atmospheric stability to investigate local environment change at an atomic power plant in Gori region. The used data was obtained from field observation of major meteorological variables such as wind direction and speed, air temperature, relative humidity, and solar radiation using AWS (Automatic Weather System) in Gori region from February to September in 2003. As a result, when strong northerly winds (more than 4~6 m/s) and weak northwesterly winds (2~3m/s) blow, atmospheric stability was neutral and stable from winter to spring, respectively. Atmospheric stability (neutral) in summer was similar to that in two other seasons (winter and spring), but it was stable for weak southwesterly winds (2~3 m/s). Finally, atmospheric stability was neutral for strong winds (over 4~6 m/s) in fall without respect to wind direction, while that was stable for very weak winds (less than 2 m/s).

Partial Sequence Analysis of Puumala Virus M Segment from Bats in Korea

Yun, Bo-Kyoung,Yoon, Jeong-Joong,Lee, Yun-Tai The Korean Society of Virology 1999 Journal of Bacteriology and Virology Vol.29 No.1

Hantavirus is a genus of the Bunyaviridae family causing two serious diseases, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Puumala virus is a member of hantavirus originally found in Europe, and its natural reservoir is Clethrionomys glareolus. It is also associated with the human disease nephropathia epidemica, a milder form of HFRS. To identify the hantaviruses in bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area in Korea, and nested RT-PCR was performed with serotype specific primer from M segment. Interestingly, Puumala virus was detected in bats (Rhinolophus ferrum-equinum) only from Won-Joo. The 327 bp nested RT-PCR product, was sequenced. The sequence database search indicates that the sequence is homologous to the published sequence of Puumala viruses. The sequence similarities were ranged from 71% to 97%. The highest sequence similarity was 97% with Puumala virus Vranicam strain, and the lowest was 71% with Puumala virus K27 isolate. Puumala virus Vranicam strain was isolated from a bank vole (Clethrionomys glareolus) in Bosnia-Hercegovina. Puumala virus K27 was isolated from human in Russia. This analysis confirms that bats (Rhinolophus ferrum-equinum) in Korea are natural reservoir of Puumala virus.

Effects of Storage Buffer and Temperature on the Integrity of Human DNA

( Yun-tae Kim ),( Eun-hee Choi ),( Bo-kyoung Son ),( Eun-hee Seo ),( Eun-kyoung Lee ),( Je-kwon Ryu ),( Gi-won Ha ),( Jin-seon Kim ),( Mi-ran Kwon ),( Jae-hoon Nam ),( Young-jin Kim ),( Kyoung-ryul Le 대한임상검사과학회 2012 대한임상검사과학회지(KJCLS) Vol.44 No.1

In this study, we have examined the effects of the storage time and temperature on DNA quality and have also studied the effects of the hydration buffer in which DNA is dissolved. This study was performed using 160 human blood samples collected with informed consent from 2007 to 2008 in the hospital where this cohort study was performed. The DNA extracted was dissolved using distilled water (DW) or Tris-EDTA (TE) buffer, and stored in the deep freezer or refrigerator for up to 10 weeks at -70℃, -20℃, 4℃, and 25℃, respectively. DNA integrity was determined by the degree of smearing of DNA on the gel. After four weeks, all of the 20 DNA samples dissolved in DW and stored at 25℃ were entirely degraded. After 10 weeks, 6 of the 20 DNA samples dissolved in TE buffer and stored at 25℃ were fairly degraded, and 4 of the 20 DNA samples dissolved in DW and stored at 4℃ were fairly degraded. The 20 DNA samples dissolved in TE buffer and stored at 4℃ were stable for 10 weeks. DNA samples stored at -20℃ and -70℃ did not appear to degrade in either DW or TE buffer, even at the 10-week point. We suggest that TE buffer should use for DNA elution, in order to protect against degradation and to preserve DNA for a long period of time, and the samples should be stored at -20℃ or -70℃.

책 페이지에 구현된 만화의 흑백 연출 의미 분석

윤보경(Yun, Bo-Kyoung) 한국만화애니메이션학회 2015 만화애니메이션연구 Vol.- No.40

만화에서 공간의 역할은 곧 영화에서 시간의 역할과 비교된다. 그렇기에 만화의 서사성은 공간과 불가분의 관계에 놓여 있고, 공간 구성이 중요성을 띠게 된다. 만화의 공간은 여러 개의 칸과 칸새가 인접, 병렬되어 다수의 프레임으로 구성되어 있는데, 디지털 만화에서는 칸과 칸 사이의 결집력이 상대적으로 약해지고 칸이 독립적인 프레임을 이루는 경향이 크지만, 페이지 만화에서는 여전히 칸이 모여 이뤄내는 장의 개념이 강조된다. 시각 매체에서 공간의 기본 틀을 구성하는 우선된 요소는 어두움과 밝음 즉 명암으로, 이는 만화 뿐 아니라 다른 시각 매체에도 고려해야 할 주요 조형 요소 가운데 하나이다. 만화에서도 이미지 구성을 위해 명암의 중요성이 강조되는데, 타매체와는 다르게 극단의 흑색과 백색의 사용이 두드러지는 것이 특이할 점이다. 매체적 측면으로는 판화를 배급 기반으로 했던 만화의 태생적 환경과, 형식적 측면으로는 만화 언어의 가독성을 바탕으로 자연스레 흑백의 이미지 사용이 이끌어졌다. 흑백 만화는 인쇄술의 발전과 컬러 만화의 등장으로 완전히 사라진 것이 아니라 여전히 창작되고 읽혀지는데, 이는 컬러 만화에서 표현하기 어려운, 흑백 만화만이 갖는 의미와 효과가 있다는 것을 뒷받침 한다. 만화의 흑백 표현은 색상에 대한 정보의 누락이라 저평가되지 않고 오히려 높은 가독성, 형식 언어의 강조와 이미지의 모던함을 불러온다. ‘만화에 있어 흑과 백은 실체적인 색이 아니라 상징적 의미를 포괄하는 복합적 요소’이며 형상의 ‘존재와 부재’를 드러내는 개념으로서 기능하고 있기 때문이다. 이 연구는 만화의 환경이 디지털화되면서 자칫 간과할 수 있는 흑백의 표현이 가져다주는 의미와 기능, 역할을 재조명하는 데에 그 목적을 두려한다. 흑백의 다양한 이미지와 형상을 통해 만화의 형식적 공간이 어떻게 구성되고 채워지며 서사 전달이 구체화 되는가를 실제의 작품을 예시로 분석하고 파악한다. The purpose of this study is to determine the meaning and function of black and white in the comic strip, which are mounted on the book pages. The role of space in comic strip, compared to the role of time in the cinema, enable to feel the role of the time in the book. Therefore storytelling of comic strip has been placed in the inseparable relationship with space. My aim is to think about the black and white meaning in the visual media, especially the use of black and white which is highlighted in the comic strip. Black and white doesn"t mean a loss of color in the comic strip but it works around 2 important notions : readability, modernity (with minimalism). In comic strip, ‘black and white’ is not a true colour but it is complex items which indicate symbolic significances. Moreover it is also a notion which enhance ‘existence and absence’ and ‘visible and invisible’. That"s why to analyze and to understand how the space is organized in comic strip page, i chose several examples extract from black and white comic strip.

Functional Implication of β-Carotene Hydroxylases in Soybean Nodulation

Kim, Yun-Kyoung,Kim, Sunghan,Um, Ji-Hyun,Kim, Kyunga,Choi, Sun-Kang,Um, Byung-Hun,Kang, Suk-Woo,Kim, Jee-Woong,Takaichi, Shinichi,Song, Seok-Bo,Lee, Choon-Hwan,Kim, Ho-Seung,Kim, Ki Woo,Nam, Kyoung He American Society of Plant Biologists 2013 PLANT PHYSIOLOGY - Vol.162 No.3

<P><I>Carotenoids are essential for nodule development in soybean.</I></P>

Effects of Storage Buffer and Temperature on the Integrity of Human DNA

Kim, Yun-Tae,Choi, Eun-Hee,Son, Bo-Kyoung,Seo, Eun-Hee,Lee, Eun-Kyoung,Ryu, Je-Kwon,Ha, Gi-Won,Kim, Jin-Seon,Kwon, Mi-Ran,Nam, Jae-Hoon,Kim, Young-Jin,Lee, Kyoung-Ryul Korean Society for Clinical Laboratory Science 2012 대한임상검사과학회지(KJCLS) Vol.44 No.1

In this study, we have examined the effects of the storage time and temperature on DNA quality and have also studied the effects of the hydration buffer in which DNA is dissolved. This study was performed using 160 human blood samples collected with informed consent from 2007 to 2008 in the hospital where this cohort study was performed. The DNA extracted was dissolved using distilled water (DW) or Tris-EDTA (TE) buffer, and stored in the deep freezer or refrigerator for up to 10 weeks at $-70^{\circ}C$, $-20^{\circ}C$, $4^{\circ}C$, and $25^{\circ}C$, respectively. DNA integrity was determined by the degree of smearing of DNA on the gel. After four weeks, all of the 20 DNA samples dissolved in DW and stored at $25^{\circ}C$ were entirely degraded. After 10 weeks, 6 of the 20 DNA samples dissolved in TE buffer and stored at $25^{\circ}C$ were fairly degraded, and 4 of the 20 DNA samples dissolved in DW and stored at $4^{\circ}C$ were fairly degraded. The 20 DNA samples dissolved in TE buffer and stored at $4^{\circ}C$ were stable for 10 weeks. DNA samples stored at $-20^{\circ}C$ and $-70^{\circ}C$ did not appear to degrade in either DW or TE buffer, even at the 10-week point. We suggest that TE buffer should use for DNA elution, in order to protect against degradation and to preserve DNA for a long period of time, and the samples should be stored at $-20^{\circ}C$ or $-70^{\circ}C$.

Enzymatic production of 3,6-anhydro-l-galactose from agarose and its purification and in vitro skin whitening and anti-inflammatory activities

Yun, Eun Ju,Lee, Saeyoung,Kim, Ji Hye,Kim, Bo Bae,Kim, Hee Taek,Lee, Sun Hee,Pelton, Jeffrey G.,Kang, Nam Joo,Choi, In-Geol,Kim, Kyoung Heon Springer-Verlag 2013 Applied microbiology and biotechnology Vol.97 No.7

<P>3,6-Anhydro-L-galactose (L-AHG) constitutes 50% of agarose, which is the main component of red macroalgae. No information is currently available on the mass production, metabolic fate, or physiological effects of L-AHG. Here, agarose was converted to L-AHG in the following three steps: pre-hydrolysis of agarose into agaro-oligosaccharides by using acetic acid, hydrolysis of the agaro-oligosaccharides into neoagarobiose by an exo-agarase, and hydrolysis of neoagarobiose into L-AHG and galactose by a neoagarobiose hydrolase. After these three steps, L-AHG was purified by adsorption and gel permeation chromatographies. The final product obtained was 95.6% pure L-AHG at a final yield of 4.0% based on the initial agarose. In a cell proliferation assay, L-AHG at a concentration of 100 or 200 μg/?mL did not exhibit any significant cytotoxicity. In a skin whitening assay, 100 μg/?mL of L-AHG showed significantly lower melanin production compared to arbutin. L-AHG at 100 and 200 μg/?mL showed strong anti-inflammatory activity, indicating the significant suppression of nitrite production. This is the first report on the production of high-purity L-AHG and its physiological activities.</P>