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한국에 서식하는 조류와 다람쥐의 Hantavirus 감염에 대한 생태학적 연구
이연태,박철희,조규봉,송재옹,박은병,최승구,Lee, Yun-Tai,Park, Chul-Hee,Cho, Kyu-Bong,Song, Jae-Ong,Park, Eun-Byung,Choi, Sung-Gu 대한미생물학회 1996 Journal of Bacteriology and Virology Vol.26 No.1
Hantavirus is the etiologic agent of hemorrhagic fever with renal syndrome (HFRS). It has been known that the natural reservoirs of Hantavirus are not only field mice but also other animals in parts of the world. In this study, to research on the host range of Hantavirus, immunofluorescent antibody against Hantavirus was investigated in wild birds from 1991 to 1992, duck from 1991 to 1992 and squirrels (Tamias sibiricus) in 1990 in Korea. The results were as follows: 1. Of total 179 wild birds of 14 species, Emberiza elegans elegans and Passer montanus dy-bowsky were antibody positive. The positive rates were 3.92% (2 out of 51) and 1.64% (1 out of 61), respectively. 2. The antibody titers of wild birds were 1:16 and 1:64 in Emberiza elegans elegans, 1:16 in Passer montanus dybowsky. 3. The positive rate of antibody in ducks was 2.3% (3 out of 129). 4. The positive rate of antibody in squrrels was 48.10% (38 out of 79). According these results, we newly showed that passer montanus dybowsky, domestic ducks and Tamias sibiricus possessed the antibody against Hantavirus.
PS ( Y - 15') 세포상에서 아보·바이러스의 플라크 형성능
이연태,황기선,박길수 대한바이러스학회 1971 Journal of Bacteriology and Virology Vol.1 No.1
The plaque techniques of Dulbecco (1952) was first applied to the titration of arboviruses in culture of chicken fibroblast cells, also Poterfield (1959) described modificatory the plaque technique for titrating the infectivity of animal viruses. Most of arboviruses multiply in mammalian cells giving a variable amount of cytopathic degeneration. Some will produce plaque quite readily a monolayer of these cells under agar and agarose but other fail to do so. At a time when efforts were being made in this laboratory to improve the sensitivity of plaque reduction in mammalian cell by arboviruses, report were published (Miles, 1963 and Suitor, 1965) coneerning plaque production with arbovirus. They showed that several arboviruses produce minute plaques on monolayer under agar and agarose because it was inhibited by sulfate polysaccharide. They further demonstrated that this sulfated polysaccharide, which is a break down product regularly present in agar or agarose, can be adsorbed to and inactivated by diethylaminoethyl (DEAE)-dextran or protamine sulfate. When these substances were added to the agar or agarose in an appropriate dose the susceptible some arbovirus produced plaques quite as large and clear cut as those of the normal strain. This paper describes experimental modification of the plaque technique and reports obtained employing different overlay systems. The cells were used Inoues a clonal line from porcine kidney stable PS (Y-15') throughout this study. This cell cultured in Inoue's or Westaway's medium in 2 oz prescription bottle until monolayer formed. Virus strains, all of the 2 group A(Chikungunya, Western equine encephalitis) and 4 group B(Dengue II and IV, West nile, Yellow fever) arboviruses tested to produce plaques. Protamine sulfate and DEAE- dextran prepared as a various in distilled water. This stock solution were sterilized by autoelave(dextran) or filtration. They were added to the melted noble agar or agarose in appropriate amounts immediately before pouring. In those cases chikungunya virus, the plaque size are nearly same in diameter with each of six overlay systems but number of plaques are slightly difference. WEE virus that plaque size shows a range from small to large. The total number of plaques show a less than two fold difference from bottle to bottle. Dengue II virus with six overlay systems are seen. The plaque size shows a range from small (0.3 - 0.4) to large (l.5 - 2.0) The total number of plaque, both large and small, show a less than two fold difference from bottle to bottle. This virus shows variation in plaque size between agar and agarose overlay. In Dengue IV virus, plaque size shows a range from small to large and number of plaques were less than three fold difference in overlay systems. In West nile and Yellow fever virus, plaque size show small and large also total plaque number were variable under six overlay systems. West nile virus was effected with DEAE dextran in agarose overlay. The addition of these chemical reagents in optimal concentrations do not inhibit plaque sizes and number, those plaques by some viruses bear polymorphic appearances.