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주부의 인터넷 쇼핑의 유용성 인지가 활용도에 미치는 영향
박미석,이유리 숙명여자대학교 2000 生活科學硏究誌 Vol.15 No.-
This study analyzes the trend of the awareness of usefulness and usage frequency of Internet shopping and closely examines the influential factors of the trend. In addition, in this study the usage rate of Internet shopping are divided into that of search and purchase, so that the relationships of variables are easily derived through a process matrix. After all, the purpose of this methodology was to provide informatized housewives and household with fundamental data by finding a way to increase the awareness of usefulness and usage frequency of Internet shopping. The major findings are follows: (1) Those who are college-educated, married and employed, positive about information household, Internet-educated, highly capable of the Internet, and comfortable with an issue of an effluence of personal data showed high awareness of usefulness of Internet shopping. (2) Those who are young, college graduate or above, and employed showed the high usage rate of search. Those who are age 20 to 30, college graduate or above, employed, and at the early stage of family life style showed the high usage rate of purchase. In addition, those who are positive about information household, high in the tendency of innovation and the awareness of time constraints, exposed to the Internet education, high in the Internet capability, and low in a burden of the Internet bills and the effulence of personal data showed the high usage rate of search and purchase. (3) An awareness of time constraints and a concern for the effulence of personal data directly influence the usage rate of purchase and indirectly influence the usage awareness of internet shopping. And an attitude toward information household' and Internet capability directly influence the usage rate of search and purchase and indirectly influence the usage awareness of internet shopping. Therefore, according to the result of process matrix, an awareness of usefulness of Internet shopping, an attitude of information household, Internet capability are the variables that influence the usage rate of Internet shopping.
인체 췌장암세포주(Capan-1)의 증식에 미치는 amiloride의 억제효과
임대관,김신,김유리,노지훈,이지현,김지연,박무인,정근옥,박건영,구자영 고신대학교 의학부 2004 高神大學校 醫學部 論文集 Vol.19 No.1
Background/Aims Cytoplasmic alkalinization induced by activation of the Na+/H+anti porter which is stimulated upon the addition of growth-promoting agents, such as insulin, epidermal growth factor, phorbol ester, plays an essential role in the initiation on cell proliferation. In the present study the effects of amiloride, a specific and reversible inhibitor of Na+/H+antiporter, on the growth of human pancreatic carcinoma cell line, Capan-1 cells was examined and the effects of 5-fluorouracil (5-FU) were also studied. Cell cycle analysis was done to examine the mechanisms for the inhibitory effects of amiloride. Materials/Methods The growth of Capan-1 cells were examined by counting cell number on two and four days treatment with 1 μM, 5 μM, 10 μM, 20 μM, 40 μM, 80 μM, 160 μM amiloride, and 0.1 ㎍/㎖, 0.3 ㎍/㎖ 5-FU, after plating Capan-1 cells into 35-mm2 plastic dishes at d density of 10x104 cells/dish. The reversibility of the effects of amiloride was examined on two day to eight days treatment with 20 μM amiloride after seeding 2×104 cells/dish. Cell cycle analysis was done on the sells after four days treatment with 20 μM amiloride. Results Amiloride significantly inhibited the growth of Capan-1 cells in a dose-dependent fashion (p<0.05). The inhibitary effect of amiloride on the growth of Capan -1 cells was firstly shown at the concentration of 5 μM, which is not so higher than the concentration of 0.1-0.2 μM attainable by administration of usual dose of amiloride (5-10㎎). Forty-eight percent inhibition of growth was found at an amiloride concentration of 20μM after 4 days treatment, and ninety-three percent inhibition of growth was found at an amiloride concentration of 160μM after 4 days treatment. The inhibitory effect of amiloride on growth of Capan-1 cells was reversible since removal of amiloride by a media change after 48 hours treatment lead to significantly more growth than amiloride treated group (p<0.05). The reversibility of growth inhibition suggests that amiloride in not a non-specific cytotoxin for Capan-1 cells. Amiloride combined with 5-FU significantly inhibited the growth of Capan-1 cells in a dose-dependent fashion compared to an amiloride or a 5-FU alone (p<0.05). After four days treatment with 20 μM amiloride, the faction of cells in G0-G1 phase, S phase and G2-M phase was 47.3%, 35.8%, 16.9% respectively in the amiloride group (20 μM), and 44.3%, 37.1%, 18.6% in the control group. showing no significant differences between the two groups. Conclusions Amiloride significantly inhibited the growth of Capan-1 cells in a dose-dependent fashion, which was reversible. The reversibility of growth inhibition suggests that amiloride is not a non-specific cytotoxin for Capan-1 cells. The concentration of 5 μM, which is not so higher than the concentration of 0.1-0.2 μM attainable by administration of usual dose of amiloride (5-10㎎), which suggests amiloride or its analogues may be used alone or in conjunction with 5-FU for the treatment of pancreatic carcinoma. Further study is needed to clarify the effects of more potent analogues of amiloride on the growth of human pancreatic carcinoma cells.
( Yu-ri Park ),( Eun-mi Kim ),( Do-hyun Han ),( Dae-young Kang ),( Sang-geon Yeo ),( Choi-kyu Park ) 한국가축위생학회 2017 韓國家畜衛生學會誌 Vol.40 No.1
A two-tube reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was designed for the rapid visual detection of the M gene of all subtypes of avian influenza virus (AIV) and the H5 gene of the H5 subtype of highly pathogenic AIV (HPAIV). The reaction carried out in two tubes in a single step at 58°C for 40 min, and the assay results could be visually detected by using hydroxynaphthol blue dye. Using M or H5 gene-specific primers, the assay successfully detected all subtypes or H5 subtypes of AIVs, including the Korean representative H5N1 and H5N8 HPAIVs. The detection limit of the assay was approximately 10<sup>2.0</sup> EID<sub>50</sub>/reaction for the M and H5 genes of H5N1 HPAIV, respectively, and was more sensitive than that of previously reported RT-LAMP and comparable to that of real-time RT-PCR. These results suggest that the present RT-LAMP assay, with its high specificity, sensitivity, and simplicity, will be a useful diagnostic tool for surveillance of currently circulating H5 HPAIVs and other subtypes of AIV in bird population, even in under-equipped laboratories.
( Yu-ri Park ),( Jae-eun Park ),( Hae-mi Yang ),( Se-hee Won ),( Hyeon Hwang ),( Eun-seo Jung ),( Kyeong-hee Lee ) 융합연구학회 2019 융합연구학회지 Vol.3 No.2
The study conducted a survey of medical and non-medical personnel living in Seoul and Gyeonggi province for about two months from April to June 2018 to explore ways to improve people's awareness on the need to include dental hygienists in the category of medical personnel and obtained the following results. Looking at the factors that affect the changes that would be made to the dental hygienists if they were to be included in the category of medical personnel, we found that people who thought it was appropriate or fair for dental hygienists to be included as medical personnel (p<0.001) and those who had a high opinion on the role of dental hygienists (p<0.001) tended to have a positive awareness on the changes that would occur after the inclusion of dental hygienists as medical personnel. Based on the above results, it is believed that the government needs to raise people's awareness on the role of dental hygienists and actively inform the public of the need for dental hygienists to be classified as healthcare professionals.
Prevalence of antibodies against hepatitis E virus in wild boars (Sus scrofa) in Korea
Park, Yu-Ri,Park, Ji-Young,Kang, Dae-Young,Han, Do-Hyun,Yoon, Hachung,Jeong, Wooseog,An, Dong-Jun,Yeo, Sang-Geon,Park, Choi-Kyu The Korean Society of Veterinary Service 2016 韓國家畜衛生學會誌 Vol.39 No.4
We determined the nationwide seroprevalence of hepatitis E virus (HEV) infection in the wild boar population in Korea. Enzyme-linked immunosorbent assay (ELISA) results showed that 42% of the 528 wild boars that were hunted between 2013 and 2014 were anti-HEV antibody positive. Furthermore, all Korean provinces showed an HEV seroprevalence between 9.8% and 51.1%, suggesting that wild boar HEV infection occurs throughout the country. Importantly, infected wild boar could act as a potential reservoir for HEV and could aid transmission to other animals and humans.