농어촌 인구 정주에 관한 환경 계획적 연구

정성찬,이길영 大田大學校 環境問題硏究所 2002 환경문제연구소 논문집 Vol.6 No.-

The settlement of population in agricultural areas helps to prevent the influx of agricultural population to metropolitan areas and also contributes to improving residential environments of large cities. Constituting a major pillar to support balanced development of a nation, it can be said to be the key task in regional planning. Recently, Korean agricultural society has gone through a tremendous change. The economic policy of urbanization and industrialization enforced in the 1970's resulted in the concentration of industries and economies mostly in cities, which inevitably led to the phenomenon of so-called "leaving countries for cities." This indicates the outflow of agricultural population from countries into cities. As a result, the development of agricultural regions has slowed down, with the population decreasing and the community becoming empty, in contrast to urban areas. Consequently, agricultural areas are facing a crisis, and it is hard even to maintain them as residential areas. Furthermore, the wide regional gap between cities and agricultural areas derived from the process of urbanization and industrialization is recognized as a big barrier to the efficient use and balanced development of the land of the nation. Therefore, plans of rearrangements of residential environments in the agricultural areas are emergingas an urgenttask, both in revitalization of decreasing agricultural areas and in balanced developmentof the national territory. However, academic investment or developmental policies up to now have been mostly concentrated on cities. Matters of agricultural areas, thus, have been neglected as secondary problems to those of cities. It is essential to view the question of the settlement of agricultural population from various perspectives and to have academic involvement. Yet, the settlement of population, the result of movement, is a very complicated phenomenon originated in the relationships between residents, the subjects of settlement, and the surrounding regional circumstances. These phenomena vary according to the behaviors and attitudes of the residents. And the degrees of satisfaction of the residents differ depending on each individual's value system. Therefore, the attempt to establish the settlement of the agricultural population can be seen in this context. It is the biggest mission, then, to simultaneously examine both aspects, the aspect regarding regional environments and the aspect related to residents, the subjects of settlement. Finally, as mentioned before, it is necessary to note that the attributes and conditions are various both in a macroscopic level involving regions and in a microscopic level involving individuals as subjects of settlement. It is also necessary to investigate the perspectives from which the settlement in the agricultural regions is dealt with in the regional, environmental planning. Obviously, this issue needs be handled with external and internal analyses as it involves both the reality and the ideal of the residents' value system. Therefore, this study is not about simply restoring the past in regard to a series of problems with the settlement of agricultural population. This study aims to pursue the natural essence of agricultural areas in which new agricultural regions and cities can be integrated under the conditions of national territory, and at the same time, to deal with the agricultural areas as new space for settlement.

등줄쥐 연수의 Tyrosine Hydroxylase, Dopamine-β-Hydroxylase 및 Phenylethanolamine-N-Methyltransferase 면역반응신경세포

정영길,이남섭,민성기 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.3

손상받은고양이 줄무늬체에서 축삭반응에 관한 미세구조적 연구

양남길,조영열,안의태,고정식,박경호,김진국 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

SM20C 旋削時 加工條件 變化에 따른 칩 形狀에 관한 硏究

정태상,황광성,김명규,김길진,박영태 진주산업대학교 산업과학기술연구소 2007 산업과학기술연구소보 Vol.- No.14

This study was to look for cutting conditions which are safe, comfortable and contributable to the increase in productivity at the time in turning. The shape of chips while turning have a strong effect on cutting processing. If chips fail to be well controlled, systematic cutting is not possible by revolution of chips entangled with workpiece or damage of tool edges, and this causes problems threatening the safety of the operator. This study is designed to identify conditions which chips effectively controlled and able to treat properly, and the results are as follows: 1. Feed rate, which determined the shape of chips, was found to have a stronger impact on the shape of resulting chips than any other cutting condition. 2. When feed rate was 0.25mm or over, controlled chips(coil spring type) were produced under whatever cutting conditions. 3. When feed rate was 0.25mm or over and depth of cut was 1.0mm, well-controlled shorter coil type chips were produced at every spindle speed, which implied they were the most suitable cutting conditions. 4. Feed rate 0.2mm was found to be an unstable section where coil spring and linear mixed type chips were produced together, and as feed rate decreased, more linear chips beyond control were produced. 5. At the same spindle speed, as the diameter of the material became bigger, controlled chips were produced only when the depth of cut was reduced, and vice versa under feed rate 0.2mm. 6. At the same feed rate, the lower spindle speed was, the more stable chips were produced.

쇄석의 진동다짐 특성에 관한 실험적 고찰

정길수,박병수,홍영길,유남재 江原大學校 産業技術硏究所 2004 産業技術硏究 Vol.24 No.B

This Study is results of experimental works to investigate the characteristics of vibro-compaction of crushed stones having coarse grain sizes. For testing material, crushed stone, sieved within very narrow ranges of grain size distribution, was used. Cyclic loading apparatus was used to apply cyclic loading to the specimen prepared in the mold. Tests were performed by changing the ratio of the maximum to the minimum stress, frequency and the magnitude of the maximum and the minimum stresses. Settlement of specimen due to cyclic loading was measured to analyze the compaction efficiency and sieve analysis test after cyclic loading test was also carried out to find the crushing rate of the specimen. As results of cyclic loading test, normalized settlement in terms of specimen height tends to be converged around loading cycle number of 1500. The magnitude of normalized settlement is in the range of 3.11 ~ 8.57%. The crushing rate is in the range of 4.46 ~ 8.78%. Normalize settlement and the crushing rate tend to increase with decreasing the ratio of the maximum to the minimum stresses and they tend to increase with increasing the frequency and the magnitude of the maximum and the minimum stresses for the given ratio. In conclusions, compaction rate of crushed stone is controlled by the dynamic stress (difference between the maximum and the minimum stresses) and the crushing rate is dominated by applied energy to the specimen.

저분자량 폴리에틸렌이민을 이용한 유전자 송달 및 발현 유효성 연구

정길재,박귀례,신지영,최한곤,오유경 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.4

Branched and linear polyethylenimines (PEIs) have been studied as efficient and versatile agents for gene delivery in vitro and in vivo. PEIs exist in a linear or branched topology and are available in a wide range of molecular weight (Mw). Most studies have been done using PEIs with Mw higher than 10Kd. This study was aimed to test the transfection efficiency and the cell viability following gene delivery using PEI of Mw 2Kd, a relatively lower Mw cationic polymer. We used murine interleukin-2(mIL-2) plasmid DNA complexed with branched PEI 2Kd or 25Kd, and transfected them into a myoblast muscle cell line, C2C12. The cellular uptake of mIL-2 plasmid DNA was determined using quantitative polymerase chain reaction. RNA transcript levels were studied in the myoblast cells. Our results show that PEI 2Kd was as effective as PEI 25Kd in celluar gene delivery and transfection efficiency in C2C12 cells. Moreover, MTT assay indicated that PEI 2Kd/DNA complexes did not significantly reduce the cell viability regardless of N/P ratios. These results suggest that PEI of Mw 2Kd might play a role as effective and low toxic nonviral vector systems for muscular cell lines.

흰쥐의 감각신경절세포에 대한 Methylmercuric chloride의 독성효과

鄭榮吉,白承和,韓斗錫,柳道坤,朴承澤 한국전통의학연구소 1998 한국전통의학지 Vol.8 No.1

흰쥐의 배양 척수감각신경절세포에 대한 methylmercuric chloride(MMC)의 세포독성을 조사하기 위하여 여러 농도의 MMC가 포함된 배앙액에서 24시간 동안 배양한 후 MTI assay법에 의하여 세포의 생존율을 조사하였다. MMC는 농도에 비례하여 세포의 생존율을 감소시켰다. 또한 여러 농도의 MMC가 포함된 배앙액에서 흰쥐의 척수 감각신경절(dorsal root ganglion, DRG) 세포를 24시간 동안 처리한 결과 MTI_(50) 값이 25μM MMC에서 나타났다. MMC는 Borenfreund등(1988)의 독성판정기준에 의하여 흰쥐의 배앙 DRG 세포에 고독성인 것으로 나타났다. 이상의 결과는 MMC가 흰쥐의 배양 척수감각신경절세포에 강한 신경독성 효과가 있음을 제시하였으며 또한 배양신경세포는 약제의 효과를 검색하는데 효과적이었다. To examine the cytotoxic effect of methyl mercuric chloride(MMC) on the cultured rat spinal dorsal root ganglion(ORG) neurons, cell viability was measured by MTT assay after rat spinal ORG neurons were incubated with media containing various concentrations of MMC for 24 hours The results were as follows ; 1. MMC decresed cell viability of rat spinal ORG neurons in a dose-dependent manner. 2. MIT_(950) value was a 25uM MMC after spinal ORG neurons were cultured for 24 hours at various concentrations of MMC. 3. MMC was heighly toxic on the cultured ORG neurons by the toxic criteria of Borenfreund et al.(1988). From above the results, it is suggested that methylmercury involves in neurotoxicity by the decrease of cell viability in cultured rat spinal DRG neurons, and cultured neurons are effective in screening the putative agents.

야생 등줄쥐 (Apodemus agrarius coreae) 간뇌의 Dopamine성 신경세포

정영길,이남섭,민성기,김무강 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.6

중등 예비교사 체육수업에서의 피드백 분석

신영길,최원준,정향순,서호성 경북대학교 사범대학부속 중등교육연구소 2003 중등교육연구 Vol.51 No.2

The purpose of this study is to analysis on the feedbacks of middle school student teachers in physical education classes during a period of practical training and to find effective feedback for enhance the quality of classes. In order to achieve the purpose of this study, data were collected through record the 9 student teachers' classes on video tape and analysed by using feedback analysis profile(FAP). The result of this study showed that the FAP of student teachers were severe and both teachers and students. First, feedback type for subject was in large part personal feedback and mainly feedback was prescriptive feedback about not correct skill, and the feedback was given during or after perform skill. Second, student teachers rather gave negative feedback to learners than positive. They showed correction complex. Third, student teachers didn't understand the content of class completely, so they didn`t show development of content and harmony between task and practice, feedback.

Ethylene Glycol Monoethyl Ether가 흰쥐 정소의 형태와 복합당질의 Lectin 결합 양상에 미치는 영향

염영옥,정길남,조운복 부산대학교 과학교육연구소 2007 교사교육연구 Vol.46 No.2

Ethylene glycol monoethyl ether(EGEE)를 투여한 휜쥐 정소의 조직학적 구조와 glycoconjugates에 대한 lectin 결합양상에 미치는 영향을 규명할 목적으로 250~300g의 Sprague-Dawley 계 수컷 흰쥐를 사용하여 대조군과 EGEE 투여군으로 나누고, EGEE 투여군은 다시 EGEE 1주, 2주, 3주 및 4주 투여군으로 나누었다. 조직학적인 변화는 H-E 및 PAS 염색으로 관찰하였고, glycoconjugates의 당잔기 변화는 avidin-biotin-peroxidase complex 법으로 biotinylated lectin (DBA, SBA, PNA, BSL-1, sWGA, RCA-1, UEA-1, Con A, LCA)를 사용하였다. EGEE 2, 3, 및 4주 투여군에서는 대조군에 비해 정소의 크기, 정세관의 직경, 정모세포와 정자세포의 수가 감소되었다. 정조세포와 버팀세포의 위축 또는 파괴가 모든 EGEE 투여군에서 관찰되었으며 이러한 변화는 투여기간이 길어질수록 심하였다. 정모세포와 정자세포는 EGEE 4주 투여군에서는 관찰되지 않았다. EGEE 투여군에서는 대조군에 비해 정조세포의 PNA, RCA-1 및 sWGA 반응성이 감소하였으며 특히 LCA, Con A의 반응은 뚜렷하게 감소하였다. 대조군에 비해 EGEE 투여군의 정모세포는 SBA는 조금 증가하고, PNA 반응은 크게 증가하였으나 LCA와 Con A의 반응은 현저히 감소하였다. 정자세포는 EGEE 투여군에서 대조군에 비해 PNA는 크게 증가하였다. 버팀세포의 PNA, UEA-1과 sWGA 반응은 EGEE 투여군에서 감소되어 소실되는 경향을 나타내었다. 사이질세포에서는 UEA-1 반응성은 소실되었으며 BSL-1 및 Con A 반응성은 EGEE 1주 투여군에서 약간 증가하였으나 Con A는 4주 투여군에서 현저히 증가하였다. 결론적으로 EGEE는 흰쥐 정소 정세관의 정세포 발생과 정자 형성 및 정소의 glycoconjugates 대사에도 심한 영향을 미치는 것 같다. This study was performed to investigate the effects of ethylene glycol monoethyl ether(EGEE) on the histological structure and glycoconjugates of seminiferous tubules of the rat. Sprague- Dawley male rats weighing about 250~300g were divided into a control and EGEE-administrated groups. EGEE-administrated groups were subdivided into 1, 2, 3 and 4 weeks of administrated group. To find the histological changes, H-E and PAS stainings were used and to investigate the changes of sugar residues of glycoconjugates, biotinylated lectins (DBA, SBA, PNA, BSL-1, sWGA, UEA-1, LCA, and Con A) were applied with avidin-biotin-peroxidase complex method. Decrease in size of testis, diameter of seminiferous tuble, number of spermatocyte and spermatid were observed in 2, 3, and 4 weeks-administrated group. The shrinkage or demolition of spermatogonia and Sertoli's cells was observed frequently in all EGEE-administrated groups. These changes became more severe as the administration continued. In 4 weeks administrated group, no spermatocyte and spermatid were found. The binding of PNA, RCA-1 and sWGA of spermatogonia tended to decrease, and the reactivity of LCA and Con A decreased significantly in the EGEE-administrated group. In the spermatocytes, SBA affinity increased slightly, PNA reaction increased abundantly, but the affinity of LCA and Con A decreased remarkably in all administrated groups compared with the control group. In the spermatid, the binding of PNA increased notably in the administrated group compared with the control group. In the Sertoli's cells, PNA, UEA-1 and sWGA affinity showed the pattern of decreasing and disappearance in the administrated groups compared with control group. In the Leydig's cells, UEA-1 affinity disappeared, BSL-1 and Con A binding tended to increase slightly in 1 week-administrated group, whereas Con A affinity increased remarkably in 4 weeks-administrated group. In conclusion, it was assumed that EGEE induced seriously toxicity which affected spermatogenesis and spermiogenesis of seminiferous tubules, and metabolism of glycoconjugates of the testis.