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이영철,이금용,김형찬,박기범,유익제,안평욱,최춘언,손세형 한국산업미생물학회 1992 한국미생물·생명공학회지 Vol.20 No.6
산업적으로 산도 17.0% 이상의 고산도 식초를 생산하기 위하여 반연속식인 1st stage와 유가식인 2nd stage로 구성된 two stage 초산 발효를 온도 30 ℃, 교반속도 600 rpm, 통기량 0.1 vvm에서 실시하였다. 1st stage에서 초기 에탄올 농도를 50.0 g/ℓ, 잔류 에탄올 농도를 5.0 g/ℓ로 정하여 반연속적으로 초산발효를 하고, 2nd stage 에서 발효시간의 경과에 따라 에탄올을 유가식으로 첨가하여 초산발효액내의 에탄올 농도를 5.0 g/ℓ에서 10.0 g/ℓ로 유지했을 때 산도가 17.6%인 고산도 식초를 생산할 수 있었으며, 또한 이 ??의 최대 초산 생산성은 3.3 g/ℓ·hr였다. The production of vinegar containing 16.0 ∼18.0% of acetic acid was examined in two stage fermentation consisting of semi-continuous and fed-batch type. The optimum conditions were obtained when the fermentation was carried out at agitation of 600 rpm, aeration of 0.1 vvm and temperature of 30℃. The initial and residual ethanol concentration in 1st stage were 50.0 g/ℓ and 5.0 g/ℓ, respectively, and the ethanol concentration in 2nd stage was maintained from 5.0 to 10.0 g/ℓ. The maximum productivity was 3.3 g/ℓ-hr and the acidity was 17.6% after the two days of acetic acid fermentation.
재조합효모 배양에서 비이온성 계면활성제가 외래 Glucoamylase 생산 및 분비에 미치는 영향
차형준,유영제 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6
비이온성 계면활성제인 Triton X-100과 Tween 80의 재조합효모 배양에서의 외래 glucoamylase 생산과 분비에 대한 영향에 대하여 조사하였다. Tween 80을 첨가한 경우 분비에의 증대 역할은 수행하지 못하였다. Triton X-100 첨가의 경우에는 세포성장에 저해가 있는 반면에 분비효율의 증대가 있어났다. Triton X-100의 세포성장 저해 효과를 방지하기 위하여 배양 1일 후에 첨가한 결과 세포성장은 저해를 받지 않았으며 분비가 증가하여 배양액으로 분비된 glucoamylase 활성이 약 12% 증가하였다. The effects of nonionic surfactants (Triton X-100 and Tween 80) on cloned glucoamylase production and secretion in recombinant Saccharomyces cerevisiae culture were studied. Even though the extracellular glucoamylase activity was increased by addition of Tween 80 due to the increase of the cell mass, Tween 80 did not play a role in the increase of glucoamylase secretion. On the addition of Triton X-100 addition, the secretion efficiency was increased while the cell growth was inhibited. Triton X-100 was added to the culture broth after 24 hr of culture to minimize the inhibition of the cell growth, and consequently the glucoamylase activity in the culture broth was increased by 12%
난소절제후 Estrogen을 투여한 흰쥐 대동맥에서 Protein Kinase C-α와 Fas 발현의 변화
이왕수,이상엽,안지현,송영빈,김학진,이광재,유재격,김상욱,김태호,김치정,류왕성 중앙대학교 의과대학 의과학연구소 2001 中央醫大誌 Vol.26 No.3
Estrogen is believed to decrease coronary artery disease. Protein kinase C-α(PKCα)appear to be important in signal conduction pathways. Estrogen treatment increases catalytic activity of PKCα,and activation of PKCαcan modulate estrogen receptor levels and responsiveness. And it was reported that activation of PKC can protect cells from apoptosis induced by Fas ligation. But the beneficial effect of estrogen on PKCαwas not clearly demonstrated. The objective of this study was to evaluate the role of PKCαafter ovariectomy and estrogen therapy in rat aorta. The results were summarized as below: 1. The significant pathological changes were not observed in the rat aorta irrelevant to ovariectomy and estrogen therapy. 2. The expression of Fas was decreased in the aorta of estrogen-treated rats than that of ovariectomized rats without estrogen therapy. 3. Increased expression of PKCαwas more marked in the aprta of estrogen-treated rats than that of ovariectomized rat without estrogen therapy. In conclusion,PKCαmay be important in signal conduation pathway on the effect of estrogen. Activation of PKCαby estrogen reduced Fas expression, suggesting that PKCαactivation may play a role in protection against atherasclerosis. Further studies are needed to elucidate the role of PKCαafter estrogen therapy.
麻黃의 사람 비점막 섬유아세포 monocyte chemotactic protein 중 MCP - 1 , MCP - 2 , 및 MCP - 3 분비에 대한 효과
김현미,이향숙,김정선,조정제,유영천,안현종,최훈,임강현 대한본초학회 2002 大韓本草學會誌 Vol.17 No.1
Recent reports have proposed that Ephedrae Herba may modulate the immune response on allergy or asthma. Human nasal mucosal fibroblasts are a rich source cytokines, inflammatory mediators, and chemokines. Chemokines are important for the recruitment of leukocytes to sites of infection, which is essential in host defense. Objectives : The objective of this study was to investigate the effect of Ephedrae Herba on the release of the chemokines such as monocyte chemotactic protein(MCP)-1, MCP-2, and MCP-3 in human nasal mucosal fibroblasts after stimulation with cytokines like tumor necrosis factor -α(TNF-α), interferon-γ(IFN-γ), and interleukin -1β(IL-1β).
YOO, YOUNG JE,Cha, Hyung Joon 한국화학공학회 1996 NICE Vol.14 No.1
It is important to understand the differences in characteristics between plasmid-harboring recombinant yeast and chromosome-integrated recombinant yeast for the design and optimization of bioprocess employing recombinant yeast. In the present study, heterologous glucoamylase gene was inserted into yeast. The glucoarnylase activity per gene copy number of chromosome-integrated recombinant yeast MMSL-CS-A-I-5 was 3 to 6 folds higher than that of plasmid-harboring recombinant yeast MIMYSUCSlA. And the genetic stability of chromosome-integrated recombinant yeast (99%) was far better compared to plasmid-harboring recombinant yeast (65). Better genetic stability and glucoamylase activity per gene copy number of chrunusome-integrated recombinant yeast can provide advantages in higher final expression level, especially in continuous culture, compared to the plamid-harboring recombinant yeast. The optimal glucose concentration for maximum expression of glucoamylase in chromosome-integrated recombinant yeast was lower than that in plasmid-harboring recombinant yeast
YOO, YOUNG JE,Cha, Hyung Joon 한국화학공학회 1996 NICE Vol.14 No.3
For the enhanced secretion of foreign proteins, the mathematical modeling for overall recombinant yeast culture considering protein secretion dynamics is important, because we can understand and predict the behavior of biosynthesis and secretion of foreign protein and can develop control strategies for feeding with these model equations. In this research, the mathematical modeling, and simulation considering protein secretion dynamics. for recombinant yeast cultures that contain multicopy plasmids or chromosomally integrated genes were performed for optimization of foreign glucoamylase production. The optimal feeding policy for maximizing glucoamylase production was suggested in fed-batch culture. By introducing this optimal feeding policy, final glucoamylase activity and productivity of fed-batch culture were significantly increased compared with those of batch culture in both recombinant yeasts.