천연항균성 물질을 이용한 Vibrio vulnificus의 살균 및 독소생성 억제효과

조성환,서일원,최종덕,전상수,라택균,정수근,강동훈 國立統營水産專門大學 附設 水産科學硏究所 1993 수산과학연구소보고 Vol.4 No.-

생선회용 해산물에 오염되어, 생식한 경우 발생되는 패혈병의 원인균주인 Vibrio vulnificus의 생육 및 독성생성을 억제할 목적으로 천연 항균제인 Grapefruit종자추출물(GPSE)을 이용하여 그 항균효과를 검토하였다. In vitro 시험결과, Vibrio vulnificus에 대한 GFSE의 생육최소저해농도는 50~100 ppm 정도이었으며, Vibrio vulnificus 균체세포틀 100 ppm 농도의 GFSE 용액으로 처리하고 전자현미경 촬영시료로 조제하여 촬영한 후, 미생물형태을 무처리대조구와 비교, 검토한 결과, GFSE 처리로 세포막 기능이 파괴되어 세포내용물이 균체외부로 유출되어 균체의 생육이 억제되고 균체내부가 빈 ghost 형태의 균체수가 증대되어 GFSE의 항균효과를 확인할 수 있었다. 한편, 생선회용 해산물에 GFSE를 근육주사하고 Vibrio vulnificus를 접종한 수조내에서 사육하여 채취한 어육부를 마쇄하여 일정량씩을 표준사료에 첨가해서 통닭용 병아리를 실험동물로 하여 사양실험을 실시한 결과. 체중증가율, 단백질이용효율, 혈청내 효소활성, 혈청의 주요성분함량 등에 있어서, GFSE 처리수조내에서 사육한 해산물을 첨가한 시료시험구의 경우, 무첨가사료처리구와 비교할 때 뚜렷한 차이를 목격할 수 없었으며, GFSE 첨가에 따른 실험동물의 성장저해 또는 독성현상을 유발하지 않음을 알 수 있었다. 아울러, 각 처리구에서 사양한 해산물의 어육부에 오염된 Vibrio vulnificus의 균수를 측정한 결과, 250ppm 이상의 GFSE 처리구에서는 Vibrio vulnificus를 전혀 검출할 수 없었다. To prevent food-poisoning outbreaks by Vibrio vulnificus the antimicrobial efficacy of grapefruit seed extract (GFSE) was examined. Minimal inhibitory concentration (in vitro) for the microorganism was found to be 50~100 ppm. Transmission electron micrographs of Vibrio vulnificus showed the biocidal action of this natural antimicrobial agent would be related to specific respiratory effect coupled with the destruction of permeable function of microbial cell membrane. After Anguilla japonia GFSE-injected to the body was incubated in the seawater contaminated by Vibrio vulnficus the fish meats were taken up, mixed with control diet and used as a diet in the feeding experiment. In this experiment the effect of GFSE treated with fish muscle on body weight protein efficiency ratio, serum enzymes and serum blood component of broiler chicks was investigated. It is proved from this study that there is neither Vibriosis nor toxicity associated with GFSE itself and fish meats treated with it when it is injected to the fish body at a level of 250 ppm or less.

均一分布荷重을 받는 三角形 Rib의 光彈性 應力 解析

趙澤東 충남대학교 공업교육연구소 1981 論文集 Vol.4 No.2

For a triangular rib loaded by uniformly distributed load, the stress distributions all over the field as well as along the some sections were investigated by a photoelastic experiment. The rib tested had a right-angular shape and the photoelastic material used to make model was epoxy resin. Experimental results showed that the stresses on horizontal and inclined boundaries were constant and the stress state was similar to that of triangular truss loaded at its end.

SOL-GEL법을 이용한 강유전체 BaTiO₃박막 및 Ceramics 제조

조채룡,이수재,장민수,김호종,정세영,노동택,김성철 동의대학교 기초과학연구소 1993 基礎科學硏究論文集 Vol.3 No.1

Concentrated solution was prepared for a sol-gel process of BaTiO₃. Barium hydroxide and Ti-isopropoxide were used as the raw materials with methanol and isopropanol as solvents. Dense ferroelectric polycrystalline BaTiO₃thin films with the perovskite structure were fabricated by spin coating of the prepared concentrated solution on a few substrates. From XRD investigation, it was confirmed that tetragonal phase of polycrystalline BaTiO₃powders are formed after heat treatment above 550℃ in air and grain-oriented BaTiO₃thin films are obtained by coating of 0.06M concentrated solution on the substrates. It was observed that surfaces of thin films deposited on the substrates by our methods were formed crack-free and uniformly. The thickness of thin film which depends the concentration of the solution was 400∼900Å for 1 time coating, which was confirmed by SEM analysis. Ceramics and thin films show dielectric constant of 4600 (at 1㎑) and capacitance 300∼800㎊ (at 1㎑) at room temperature, respectively.

大邱地方에서 分離한 Salmonella의 菌型 및 抗菌劑耐性(1973-1980)

趙東澤 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

1973年 1月부터 1980年 12月까지 大邱地方에서 腸티프스 및 類似患者로부터 分離한 1,612株의 Salmonella 菌型의 年度別 變遷은 S. Paratyphi A가 S. typh에 比하여 漸次 增加되는 追勢를 보였다. 分離된 Salmonella 菌의 藥劑耐性檢査 結果 S. Paratyphi A가 1株, S. typhi가 51株로서 耐性株였으며 그中 15株의 S. typhi는 R-plasmid에 의한 傳達性 耐性株였고, 특히 1980年에는 Cm, Su, Ap, Ts, 에 耐性인 1株의 S. typhi가 分離되었다. During the period from January 1973 through December 1980, a total of 1,612 strains of Salmonella were isolated from patients suspected of typhoid fever, and the distribution of types by years and the resistance to antimicrobial drugs were studied. There was a tendency that the isolation frequency of Salmonella paratyphi A is steadily increasing as compared with the frequency of S. typhi. In 1980, 27 strains of S. paratyphi A and 65 strains of S. typhi were isolated. One strain of S. paratyphi A and 51 strains of S. typhi were resistant to one or more drugs tested. Among the drug-resistant S. typhi, 15 carried R plasmids causing resistance to chlormaphenicol, tetracycline, streptomycin, sufisomidine, ampicillin, and/or kanamycin. One strain of S. typhi isolated in 1980 was resistant to chloramphenicol, sulfisomidine, ampicillin, and trimethoprim-sulfamethoxazole, and the complete resistance was transmitted to E. coli.

開業醫 硏修敎育에 關한 調査 硏究

沈雲澤,李棟培,曺永釵 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.2

For the purpose of analyzing the opinions of medical practitioners who already took lectures of medical practitioner training seminars, we conducted the surveys from April I through June 30, 1984. Total subjects were 472 nationwide medical practitioners and their opinion about present training seminar system and about improvement of that in the future were analyzed. Followings are the conclutions therefrom: 1. Of the lectures of medical practitioner training seminar, 47. 5% were sponsered by provincial medical society, 25. 5% were scientific lecture meetings of sectional society and 21.5% were professional chair of medical college. 61. 9% of subjects responsed "may be acceptable", 14.6% were "difficult to grasp the content of lecture" and "no interest", but 6. 9% responsed "interest" 2. About the effect of lectured training seminar, 40.2%, of subjects responsed "clinically" practicable", 34.8% were "modern knowledge but clinically cannot practicable" and 22:0%. were "too theoretical to clinical use" in that order. 3. Only 62. 7% of sudjects knew the scores acquired after training seminar. 59.7% of subjects didn't know the regulation on the shortage of aqcuired scores. 4. For the reason that obstacles about the lecture of training seminar, 47.5% were lack of time, 22. 1% were inappropriate lecture place and 16. 1% were not good details of seminar. 5. As for the opinion about the contributions of lectured seminar, the follow: Acquisition of knowledge about modern medicine and drug 44. 5%, reinforcement of knowledge about clinical medicine 20.1% helpful in the clinical use 12.9,% and promote mutual friendship 6.2%. 6. The places of training seminar wanted by subjects were as it is with 35.5%, lecturing tour to the Eup and Myon with 27.8%, university hospital and training hospital with 20.8%, establish the training course to the university hospital with 13. 3%. 7. Details and methods of training seminar were distribute the teaching material with 42.2%, for the practical technique with 30. 1%, theoretical lectures with 24.6%. And the fields of training seminar were without consideration of major field were 53.4%, consideration of major field only with 40.9%. 20. 4% wanted October for the time of training seminar, 16.3% on November, other months were revealed lower rate.

肺結核患者의 難治化에 미치는 要因에 關한 調査硏究

沈雲澤,李棟培,曺永釵 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

To find out the factors affecting to the intractability of pulmonary tuberculosis, we analyzed the 1,047 pulmonary tuberculosis patients, registered and treated at the Clinic of Chungnam Branch of Korean National Tuberculosis Association for entire 8 years from January 1st, 1978 to the December 31st, 1985. Following variables were find out to be the affecting factors to the intractability of pulomonary tuberculosis. 1. General characteristics : age of patient, residential area, education, occupation, marital status and smoking status; above 30 years of age, rural area, low educational background, physical workers and heavy smokers were more apt to be intractable pulmonary tuberculosis patient. 2. Laboratory findings : chest P-A, direct sputum smear and culture, and rate of antituberculous drug resistance; moderately advanced and far advanced, smear and culture highly positive and higher occurrence rate of antituberculotic resistance. 3. Medical facility they treated : primary and retreatment; the patient who treated at the drug store and health center were find out: more easily become intractable pulmonary tuberculosis patient.

Congo Red에 의한 이질균 세포막 단백의 변화

강동민,이상화,이유철,설성용,조동택 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.1

To study the correlation between virulence and Congo red binding capacity of Shigella, 2 invasive strains of Shigella flexneri to HeLa cell were subjected to investigate the regulation of levels of membrane proteins mediated by Congo red. Two tested strains were pigmented on the agar plate containing Congo red. Virulent S. flexneri grown in the presence of Congo red at 37℃ showed increased levels of three proteins with molecular weight of 43,000, 58,000, 63,000 daltons in the sodium lauryl sarcosinate(SLS) soluble membrane fractions and those proteins were also found in the total membrane fractions. But those proteins were not found in the SLS insoluble fractions. At 32℃ or 42℃, the protein levels in the SLS soluble fractions or SLS insoluble fractions unaffected by the presence of Congo red. The expression of these proteins was dependent to the incubation temperature.

콩 종간교잡에서 주요형질의 유전분석

이정동,권택화,조호영,황영현 慶北大學校農業科學技術硏究所 2002 慶北大農學誌 Vol.20 No.-

재배종과 야생종의 인공교잡 분리세대에서 주요형질들의 변이와 유전력을 조사하여 야생종을 이용한 콩 품종육성의 기초자료를 얻고자 수행한 결과를 요약하면 다음과 같다. 두 인공교배의 F_2 집단에서 100립중을 제외한 나머지 조사 형질들은 초원분리를 보였다. F_1과 F_2의 평균 개화일수는 양친의 중간정도로 정규 분포를 보였다. 백립중의 F_1 평균은 은하콩, 소백나물콩 두 조합 각각6.5, 5.7g으로 소립종 쪽으로 치우친 정규분포를 보였다. F_2의 평균수량은 양친의 중간정도이었다. F_1의 덩굴성은 두 조합 각각 6.4, 6.6이었으며 F_2 평균은 은하콩 조합이 6.0으로 덩굴성 쪽으로 치우쳐 덩굴성이 부분적인 우성으로 보였으나, 소백나물콩 조합에서는 4.6으로 은하콩과는 다른 결과를 보였다. 경장, 개회일수, 개체당 협수, 수량, 도복, 덩굴성 등은 높은 광의의 유전력을 보였으며 100립중은 협의의 유전력이 은하콩 조합에서 52.3%, 소백나물콩 조합에서 65.6%로 평가되어 초기세대에서의 선발이 가능한 것으로 나타났다. This study was conducted to obtain the information of varietal development using wild soybean through investigation of variation and heredity of major agronomic characters in F_2 generation of interspecific cross between Glycine max and G. soja. In segregating populations of two crosses, all characters observed except 100-seed weight showed transgressive segregation. Days to flowering showed normal distribution; mean days to flowering in F_1 and F_2 was approximately mean of parent. Mean of F_1 for 100-seed weight was 6.2g and 5.7g for Eunhakong/KLG10084 and Sobaegnamulkong/KLG10084, respectively, which were somewhat skewed distribution to small seeded parent. Mean of F_2 was about mean of two parents. Degree of vine was 6.4 and 5.7 in F_1 for two crosses but it was 6.0 in F_2 for Eunhakong/KLG10084, which revealed the degree of vine as partial dominance while 4.6 for Sobasgnamulkong/KLG10084, somewhat different results from the previous cross. Broad-sense heritability(h^2B) for plant height, days to flowering, pods per plant, seed yield, and degree of vine was comparatively high and narrow-sense heritability(h^2N) for 100-seed weight which is the most important character in the development of small seed-size sprout soybean was 52.3% and 65.6% for Eunhakong/KLG10084 and Sobaegnamulkong/KLG10084, respectively, which indicated that selection for the character in early generation was possible.

공단 근로자들의 혈증 중금속 농도에 관한 비교 연구

심운택,이동배,조영채,이영수,윤창규 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

For the purpose to estimate the exposure levels of heavy metals and to present a basis of occupational health managements to the industrial workers exposed to heavy metals, this study conducted to know the lead and chromium levels of whole blood of industrial workers. The study population were divided into three groups to the exposure levels ; directed exposure group, indirect exposure group and non-exposure group, and the sample number of workers who exposed to lead were 80, chromium were 77, and control group were 31. The contents of lead and chromium were analyzed by atomic absorption spectrophotometer. The results were summerized as follows : 1. Sex distribution of studied group were 77.1 % in male, and 22.9 % in female. Age distribution were 74.4% in 30-49 age group. The work in years were 43.1 % in 5-10 years, 29.8% in 1-4 years. 2. Mean blood level of lead were 33.53㎍/㎗ in direct exposure, 23.84㎍/㎗ in indirect exposure group and 11.77㎍/㎗ in control group. 3. Mean blood levels of chromium were 1.9㎍/㎗ in direct exposure, 1.51㎍/㎗ in indirect groups and 0.95㎍/㎗ in control group. 4. Mean lead levels of direct exposure group was higher in male than female as 36.44㎍/㎗, 27.35㎍/㎗ respectively, but there was no difference in indirect and control group by sex. Mean chromium levels of direct exposure group were higher levels 2.26㎍/㎗ in female than 1.84㎍/㎗ in male, but there were no difference in indirect and control group by sex. 5. The age group of 29 or younger among the direct exposed had high level of lead and chromium as 35.7㎍/㎗ 2.41㎍/㎗ respectively, but that age group had no evident difference in the level of lead and chromium among the indirect exposed and non-exposed.

산업근로자들의 소음성난청 발생에 관한 조사연구

이동배,조영채,심운택 忠南大學校 環境問題硏究所 1988 環境硏究 Vol.6 No.1

We surveyed 42 industries in Chungchong province for measurement of noise level with hearing test of 1,474 workers from there. 8.1% of total 1,474 workers had their hearing loss at over 45dB in 4000Hz. Mining occupied the highest percentage hearing loss with 11.5%. There showed a tendency of the increased occurrence of hearing loss with the career of service and the age of the workers. And also, C??-dip phenomenon was seen.