Quantitative Analysis on Air-Dispensing Parameters for Manufacturing Dome Lenses of Chip-on-Board LED System

Jeon, Eun-Chae,Lee, Je-Ryung,Je, Tae-Jin,Choi, Doo-Sun,Ham, Young-Bog,Lee, Eung-Sug,Choi, Sang-Kyu,Kim, Hwi Korean Society for Precision Engineering 2014 International Journal of Precision Engineering and Vol.15 No.11

A COB(chip-on-board) LED(light emitting diode) system has advantages such as low cost and good heat dissipation. LED chips are bonded on PCB(printed circuit board) directly without packaging process in case of COB LED systems. Dome lenses made of polymeric resin are generally covered on the bonded LED chips by air dispensing for preventing mechanical damage and making white light. However, it is hard to control the shape of dome lenses precisely due to viscosity of the polymeric resin. We analyzed the relationship of the shape of dome lenses and experimental conditions of the air dispensing in this study. We introduced a new parameter, air pressure * dispensing time, whose physical meaning is impulse per unit area, and obtained much clear relationship of dispensing parameters and shape parameters of the dome lens. This relationship was similar to viscoelastic behavior. The aspect ratio (height over diameter) was increased as the new parameter increased, and was converged to a certain value. The maximum aspect ratio could be calculated by Young's equation of contact angle.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

치주인대 세포의 교원질 생성에 대한 Substance P의 효과

전준영,최제용,경희문,성재현 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.1

Substance P는 교정력이 가해진 치아의 치주인대 중 인장력을 받는 부위에 많이 분포하는 neuropeptide 중의 하나이며, 또한 여러 조직에서 neurogenic inflammation을 야기하는 neuropeptide 중의 하나로도 알려져 있다. 그러나 중요한 세포의 단백기질인 교원질의 생성에 대한 Substance P의 효과는 잘 알려져 있지 않다. 따라서 이 연구의 목적은 배양 치주인대 세포에서 교원질 생성에 대한 Substance P의 효과를 평가하는 것이었다. collagenase-digestion method로 교원질 생성을 평가하였고 mRNA 수준에서 작용효과를 평가하기 위하여 Northern blot hybridization을 시행하였다. 이 연구는 또한 교원질 생성에 대한 prostaglandin과 gelatinase 생성도 포함하였으며 변성된 교원질의 분해를 평가하기 위하여 Zymography를 이용하였다. 비교원성 단백질, 교원성 단백질, 상대교원질에 대한 dose-dependent effect를 보면 Substance P는 비교원성 단백질 합성을 증가시켰으나 교원성 단백질 합성은 감소시켰다. 그리하여 총 단백합성에 대한 상대적인 교원질 생성을 나타내는 상대교원질은 7%에서 3.6%로 감소시켰다. 세포를 indomethacin과 동시에 처리할 때 substance P의 교원질 합성 억제효과는 나타나지 않았다. 이것은 Substance P의 교원질 합성 억제효과가 prostaglandin의 생성 때문이라는 것을 의미한다. Substance P의 교원질 합성 억제효과가 procollagen mRNA의 정상(steady-state)수준에 부합하는가를 평가하기 위하여 northern blot hybridization을 시행한 결과 Substance P는 α1(1) procollagen mRNA의 양적 변동을 일으키지 않았다. Substance P의 교원질 생성 억제효과는 전사이후의 어떤 단계에서 이루어지는 현상임을 나타낸다. 치주인대세포에서 gelatinase 생성에 대한 Substance P의 효과를 알아보기 위하여 zymography를 이용하였다. zymogram을 보면 Substance P는 치주인대세포에서 gelatinase 생성에는 아무 효과도 나타내지 않음을 알 수 있다. Substance P의 교원질 생성 억제효과가 치주인대세포에 대해 선택적인가 아닌가를 알아보기 위하여 MC3T3-E1세포를 이용하였는데 Substance P는 MC3T3-E1세포의 교원질 합성에는 영향을 미치지 않았다. 이상에서 Substance P는 인간의 치주인대세포에서 교원질 합성을 억제하였다. 이 효과는 procollagen mRNA와 gelatinase 생성의 정상(steady-state) 수준의 변화 때문이 아니라 prostaglandin 생성과 연관이 있음을 알았다. Substance P is one of the neuropeptide which presents highly in tension site of periodontal ligament during the orthodontic tooth movement. It has been also known as one of the neuropeptides which cause neurogenic inflammation in various tissues and organs. However, there is no report about the effect of substance P on major extracellualar matrix protein, collagen production. The purpose of this study was to evaluate the collagen production by substance P in human periodontal ligament cell, The collagenase-digestion method was used to evaluate collagen production and also used Northern blot hybridization for the evaluation of collagen mRNA level. This study also included in terms of prostanglandins and gelatinase production with respect to collagen production. For the collagen degradation, zymography was used to estimate denatured collagen degradation. Dose-dependent effect of substance P on noncollagen protein, collagen, and percent collagen was that substance P increased noncollagen protein synthesis, but decreased collagen systnisis. So the percent collagen, which determined by relative collagen production against total protein production, was decreased from 7% to 3.6%. This inhibitory effect of substance P on collagen production was disappeared when cells were treated concomitantly with indomethacin. It means that substance P-induced inhibitory effect on collagen production was due at least in part to the production of prostaglandins. To evaluate whether substance P-induced inhibitory effect on collagen production is correspond to the steady-state levels of procollagen mRNA, Northern blot hybridizartion was performed and it showed that substance P has no effect on the steady-state level of α1(1) procollagen mRNA. It means that the inhibitory effect of substance P on collagen production was due to the change of a certain mechanism after posttranscription. In this context, gelatinase production by substance P in periodontal ligament cells was evaluated by zymography. Zymogram showed that substance P has no effect on gelatinase production in periodontal ligament cells. To explore wheter substance P-induced inhibitory effect on collagen production is selevtive in periodontal ligament cells or not, MC3T3-31 cells which originated from mouse calvaria was used. It showed that substance P has no effect on collagen production in MCDTD-E1 cells. Taken together, substance P inhibits collagen production in human periodontal ligament cells. This effect was not due to the change of the steady-state level of procollagen mRNA and gelatinase production, but due at least in part to the change of prostaglandins production.

혼합방식을 사용한 OFDM의 PAR 경감방식의 성능 해석

장은영,전제훈,김성곤,변건식 동아대학교 정보기술연구소 2003 情報技術硏究所論文誌 Vol.10 No.2

OFDM should be used for the fourth generation communication for high speed communication. Because of high spectral efficiency and high tolerance to fading channel, OFDM is applied to many high speed wire and wireless communication such as DAB(Digital Audio Broadcast), DVB(Digita1 Video Broadcast), IMT 2000 etc. Inter-modulation, however, is derived from PAR(Peak to Average Power Ratio) of OFDM signals. The paper describes PTS(Partia1 Transmit Sequence) and SLM(Se1ect Mapping) of an existing methods which can reduce PAR. And then this papers proposed the new method that is called "Combine method". The method proposed in this paper is to combine PTS and SLM. As a result of the simulation, Combine PAR method is better than the existing methods.

ICP(Inductively Coupled Plasma) 처리에 따른 n-type SiGe/Metal contact의 SBH(Schottky Barrier Height) 연구

김이곤,장호원,전창민,송영주,강진영,심규환,제정호,이종람 대한금속재료학회 2005 대한금속·재료학회지 Vol.43 No.2

The effect of surface treatment of n-type SiGe using the inductively coupled plasma (ICP) was studied by current-voltage and x-ray photoemission spectroscopy measurements. The ICP treatment produced surface oxides and point defects at the surface of SiGe. The x-ray photoemission spectroscopy measurements showed that atomic ratio of Ge/Si was increased after the etching treatment. These results provide the evidence that Si vacancies were produced at the etched surface. Si vacancies acting as donor for electrons resulted in shift of Fermi level to near the conduction band. As a result, Fermi level could be pinned at such Si vacancies, leading to the remarkable reduction of Schottky barrier height and the reduced dependence of Schottky barrier height on metal work function. (Received September 7, 2004)

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

북한 벼 품종의 지대별 출수반응

양원하,김덕수,전용희,조영찬,김제규,이문희 한국국제농업개발학회 2004 韓國國際農業開發學會誌 Vol.16 No.1

북한벼의 출수 반응을 검토하기 위하여, 작물시험장 인공기상실과 표고가 다른 수원, 철원, 진부 등 3 지역에서 이앙기를 달리하여 1998년과 1999년에 시험 수행한 결과를 요약하면 다음과 같다. 가. 지대별 이앙기 변동에 다른 출수일수 변화는 표고가 낮은 지역일수록, 이앙기가 늦어질수록 출수일수가 짧아졌으며, 지역과 이앙기 이동에 따른 경향을 살펴보면, 표고가 높은 진부지역에서 이앙기가 늦어질수록 출수일수가 단축되는 정도가 뚜렷하게 나타났다. 2. 북한 벼의 기본영양생장성은 조생종은 대조 품종인 오대벼보다 짧은 편이었고, 중생종은 오대벼에 비하여 긴 편이었다. 다. 감온성은 대비품종인 오대벼보다 대체로 예민한 것으로 나타났으며 극조생종인 올벼 2호는 감온성이 매우 예민한 반응을 보여 ′98년에는 42일, ′99년에는 39일이었다. The experiment was carried out to determine heading response of North Korean rice cultivars at NCES(national crop experiment station). RDA. South Korea in 1998 and 1999. This experiment was tested at three different locations which were Suwon, Cheolwon and Jinbu with 5 transplanting times with 10 cultivars. Duration of heading stage of the rice plant was shortened at lower altitude and in later transplanting date, respectively. Bases on the heading days, it was classified as the basic vegetative phase(BVP) and thermo-sensitive phase(TSP) in tested cultivars. BVPs and TSPs among treated varieties were grouped with four groups. BVPs of North Korean rice cultivars for early maturity were shoter than that of Odaebyeo of Korean rice cultivars, however, those for medium maturity were longer than that of the control cultivar, Odaebyeo. Meanwhile, TSPs of North Korean rice cultivars were more sensitive than that of the check cultivar of Odaebyeo, Correlation between 1998 and 1999 was more significant in basic vegetative phase than in thermo-sensitive stage. Therefore, the heading time of the rice plant primarily depended on the period of basic vegetative phase growth.

Inhibitory Effect of Agrimonia pilosa Extract of Apoptosis by Amyloid Beta Treatment in Neurocellular HT-22

Je Hun Choi(최재훈),Yong Rae Cho(조영래),Yun Ji Lee(이윤지),Kwan Woo Kim(김관우),Da Hye Yoon(윤다혜),Je Seung Jeon(전제승),Young Seob Lee(이영섭),Dae Young Lee(이대영) 한국약용작물학회 2023 한국약용작물학회 학술대회논문집 Vol.2023 No.1

Properties of a Plasmid-Encoded Extracellular Nuclease of Serratia marcescens Purified from E. coli JM 107 Transformant

Jeon, Young Hwan,Kim, Kee Hong,Kwon, Heun Young,Shin, Yong Chul,Cho, Moo Je,Lee, Sang Yeol 慶尙大學校 기초과학연구소 1992 基礎科學硏究所報 Vol.8 No.-

S. marcescens의 세포외 nuclease gene이 cloning된 E. coli JM 107 균주로부터 nuclease를 순수분리하여 이 효소의 생화학적 특성을 연구하였다. S. marcescens의 열불활성화를 실험한 결과, 열처리의 시간에 대하여 1차 반응을 나타내었으며 60℃ 까지의 열처리에는 비교적 안정함을 알 수 있었다. 이 효소는 cofactor로서 Mg^2+이나 Mn^2+을 요구하였으며 최대 활성은 1mM의 Mg^2+ 존재하에서 나타났고, 다른 금속이온으로는 이러한 Mg^2+ 이나 Mn^2+의 요구를 대체할 수 없었다. 이 효소는 pH6에서 10사이에서 안정하였고, 최대 활성은 pH 8 부근에서 나타났다. 이 효소는 trypsin및 S. marcescens가 세포 밖으로 분비하는 Serratia의 세포외 protease에 의하여 전혀 분해되지 않았다. 그러나, 열처리에 의하여 구조가 변성된 nuclease는 이들 단백질 분해 효소에 의하여 완전하게 proteolysis 됨으로서 단백분해에 대한 저항성은 nuclease 효소의 구조적 특징에 기인함을 알 수 있었다. 또한, 대조실험으로 사용한 bovine serum albumin은 같은 실험조건에서 1/10의 trypsin만을 처리하여도 완전하게 분해되었다. 이 효소의 활성은 1mM EtBr의 첨가에 의하여 상당하게 저해되었다. We have previously described the purification procedures of the extracellular nuclease of Serratia marcescens from a recombinant E. coli JM 107 (Jeon and Lee, 1992). Using the purified nuclease, we examined the various biochemical properties of this enzyme. Kinetic parameters of thermal inactivation followed the first order kinetics. Heating the nuclease to 60℃ or higher temperature reduced enzymatic activity. It required 1mM Mg^2+ or Mn^2+ ions for its maximal activity and no other metal ions can be replaced for the cofactor require ment of this enzyme. It was stable at pH in the range of pH 6 to 10, and its maximal activity was exhibited around pH8. It was resistant to proteolysis by trypsin and an extracellular protease produced from S. marcescens. However, heat-denatured nuclease and bovine serum albumin were completely degraded when they were treated with the same proteases at the same assay conditions. Its activity was significantly inhibited by the addition of 1mM ethidium bromide.

Substitution of Heavy Complementarity Determin-ing Region 3 (CDR-H3) Residues Can Synergistically Enhance Functional Activity of Antibody and Its Binding Affinity to HER2 Antigen

Young Je Yoo,Seung Kee Moon,So Ra Park,Ami Park,Hyun Mi Oh,Hyun Jung Shin,Eun Ju Jeon,Seiwhan Kim,Hyun June Park,Young Joo Yeon 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.3

To generate a biobetter that has improved therapeutic activity, we constructed scFv libraries via random mu-tagenesis of several residues of CDR-H3 and -L3 of hu4D5. The scFv clones were isolated from the phage display libraries by stringent panning, and their anti-proliferative activity against HER2-positive cancer cells was evaluated as a primary selection criterion. Conse-quently, we selected AH06 as a biobetter antibody that had a 7.2-fold increase in anti-proliferative activity (IC50: 0.81 nM) against the gastric cancer cell line NCI-N87 and a 7.4-fold increase in binding affinity (KD: 60 pM) to HER2 compared to hu4D5. The binding energy calculation and molecular modeling suggest that the substitution of residues of CDR-H3 to W98, F100c, A101 and L102 could stabilize binding of the antibody to HER2 and there could be direct hydrophobic interactions between the aromatic ring of W98 and the aliphatic group of I613 within HER2 domain IV as well as the heavy and light chain hydrophobic interactions by residues F100c, A101 and L102 of CDR-H3. Therefore, we speculate that two such interactions were exerted by the residues W98 and F100c. A101 and L102 may have a synergistic effect on the increase in the binding affinity to HER2. AH06 specifically binds to domain IV of HER2, and it decreased the phosphorylation level of HER2 and AKT. Above all, it highly increased the overall level of p27 compared to hu4D5 in the gastric cancer cell line NCI-N82, suggesting that AH06 could potentially be a more efficient therapeutic agent than hu4D5.