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      • KCI등재후보

        핸드스프링 몸펴 앞공중1회 비틀기 동작의 소요시간 및 각운동량 분석

        권오석,윤양진,서국웅 한국운동역학회 2002 한국운동역학회지 Vol.12 No.2

        남자 기계체조 국가대표 선수 3명과 대학선수 3명을 대상으로 핸드스프링 몸펴 앞공중1회 비틀기 동작을 구간 및 국면별로 분석하고 동작의 숙련도에 따라 역학적 변인이 집단 간 어떠한 차이를 보이는지를 구명하기 위하여 Kwon3D 프로그램을 활용하여 분석한 결과 다음과 같은 결론을 얻었다. 시간요인에서 보폭을 크게 딛는 것이 손을 빠르게 지지 할 수 있고, 핸드스프링 체공시간은 짧게 하여야 발구르기의 압력을 높여 비틀기동작에서 체공 소요 시간이 길어지므로 비틀기 수행의 완성도를 높일 수 있는 것으로 나타났다. 각운동량 요인에서 기술수행 시 전체적으로 좌우(X)축에 대한 각운동량이 전후(Y)축과 수직(Z)축에 대한 각운동량에 비해 더 큰 값을 보였다. 좌우 축 각운동량은 지면에서 이지되어 동작을 수행하는 핸드스프링회전과 앞공중1회 비틀기에서 숙련자가 상대적으로 더 큰 각운동량 보이는 것으로 나타났다. The purpose of this study were a two-fold: ⑴ to analyze event and phase of handspring salto forward stretched with turn; ⑵ to know the differences in the kinematic variables between two groups. A Kwon3D program served for the estimation of this study. The group was divided into three National representative and three well-trained calisthenics in this study. The results of this study revealed that ⑴ the forward somersault performance was increased when duration time in the air was long during the salto forward stretched that the duration time of Handspring is short, the pressure at takeoff is high, the stride is large, and hands are supporting on the ground quickly; ⑵ comparing the angular movement of anterior and posterior y axis and vertical z axis, the angular movement of right and left x axis was higher during the performance. As a result, the national representative players showed better performance in Handspring salto forward stretched with turn.

      • KCI등재

        연 X-선 현미경을 이용한 금 나노입자 세포영상

        권영만,김한경,김경우,김선희,윤홍화,천권수,강성훈,박성훈,정선관,윤권하 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

        연 x-선 현미경은 ‘물의 창’ 영역(2.3~4.4 nm)의 파장을 이용하여, 수십 nm의 분해능으로 세포를 파괴하지 않고 살아있는 상태에서 세포의 내부구조를 관찰할 수 있어 가시광선현미경과 전자현미경을 단점을 보완하는 특징을 갖는 세포 생물학 연구에 적합한 현미경이다. 그러나 기존 연 x-선 현미경은 광원으로 방사선 가속기를 이용하기 때문에 사용이 제한적이었다. 이에, 본 연구에서는 2.88 nm의 연 x-선을 광원으로 사용하는 소형 연 x-선 현미경을 이용하여, 내포작용에 의해 금 나노입자를 포획한 HT1080과 MDA-MB 231 세포의 영상을 약 60 nm 분해능으로 획득하였다. 금 나노입자의 세포에 대한 독성을 제거하기 위하여 폴리에틸렌 글리콜을 캡핑하였고, 2.88 nm 파장의 연 x-선에 대하여 충분한 조영효과로 인하여 세포영상에서 뚜렷한 대조도를 나타내었다. 내포작용에 의해 액포에 포함되어 있는 다양한 크기의 금 나노입자 군집을 확인하였으며, 세포내부의 액포의 분포상태도 관찰할 수 있었다. 따라서 고분해능을 가진 소형 연 x-선 현미경을 이용하여 금 나노입자를 세포내의 미세기관이나 특정 단백질에 표지하면 연 x-선에 대한 조영효과의 증가에 의하여 더욱 유용한 정보를 획득할 수 있을 것으로 생각한다. A compact soft x-ray microscope operated in the ‘water window’ wavelength region (2.3~4.4 nm) was used for observing cells with nano-scale spatial resolution. To obtain cellular imaging captured with colloidal gold nanoparticles using a compact soft x-ray microscope. The colloidal gold nanoparticles showed higher contrast and lower transmission more than 7 times than that of cellular protein on the soft x-ray wavelength region. The structure and thickness of the cell membrane of the Coscinodiscus oculoides (diatome) and red blood cells were seen clearly. The gold nanoparticles within the HT1080 and MDA-MB 231 cells were seen clearly on the soft x-ray microscopy. The gold nanoparticles were aggregated within vesicles by endocytosis.

      • KCI등재

        현행 중등학교 과학 실험 , 실습 교육 실태 조사 및 그 운영 진단 (Ⅱ) : 고등학교 과학 실험 , 실습 교육을 중심으로

        김영호,이윤종,정원우,강용희,양승영,윤성효,안병호,윤일희,권용주,임성규,오철한,기우항,전명남,김중욱 한국과학교육학회 1998 한국과학교육학회지 Vol.18 No.3

        본 연구는 96년도에 실시한 우리 나라 중학교 과학 실험·실습 교육의 실태 조사 연구에 따른 계속 연구의 일환으로 고등학교 과학 실험·실습 교육의 실태를 조사하기 위한 것이다. 연구내용은 고등학교 과학 교과서의 분석, 실험·실습 실시 현황 분석, 현행 실험·실습 교육의 문제점 분석, 현행 학교 구성원들의 실험·실습 교육 운영 실태 조사 및 현행 실험·실습 교육의 개선을 위한 연구과제 도출을 주요 연구내용으로 다루었다. 연구방법은 전국의 고등학교 80개교를 대상으로 이들 학교의 학생 1,977명, 교사 165명, 학교장 80명에게 적용하였다. 본 연구에서 밝혀진 결과를 중심으로 우리 나라 고등학교 과학 실험 실습 교육의 정상화를 위한 실험 내적인 연구과제를 제시하면 다음과 같다. (1)교재 개발에 대한 연구, (2)실험 킷트 개발에 대한 연구, (3)교사교육 및 재교육 프로그램 개발에 대한 연구, (4)교실 밖 실험·실습 활동 프로그램 개발에 대한 연구, (5)과학 실험·실습 소재의 개발에 대한 연구. This study was accomplished to analysis and survey on the experimental and practical science education of high school in korea for the consecutive study of the an analysis and survey on the experimental and practical science education of middle school in korea(Lee, Yoon-Jong et al., 1997). The status of facilities, management for the experiment, practices, teaching methods in high schools have been investigated. The present status and reasonable management of the high school science education have been grasped from the questionaires. To do this 165 high school science teachers, 1977 students and 80 principals of high schools in Korea are administered questionaires of Science Education Research Institute of Kyungpook National University(1997). The results of this study are as follows : The reasonable management for experiments and practices of science education were scanty in the high school around the urban and rural school owing to the shortage of facilities and equipments, crowded class, excessive class works for teacher, excessive contents of present textbooks and insufficiency of the administrative supports etc. The current teaching method of high school science has emphasized knowledge. This fact does not satisfy the objective of learning due to lack of the teaching method. Desirable directions for the improvement of present status of high school science education were proposed in this paper.

      • 비피리딘과 금속이온을 이용한 기능성 포접체의 생성에 관한 연구

        권윤정 건국대학교 산업기술연구원 2000 건국기술연구논문지 Vol.25 No.-

        The crystal structure of the title complexes was determined by X-ray diffraction measurements. Tightly packed porphyrin molecules stack to form arrays of parallel channels in which a remarkable variety of guest species are accommodated. Construction of inner cavities in the solid composed mainly of organic compoments is attractive since function of the inner cavity become designable. This paper reports two kinds of complexes composed of metallic ion and bipyridine(bpy) when Cd2+ was treated with bay in different composition of solvents. Identification of the features of the molecular structure responsible for the versatility of tetraarylporphyrin-based clathrate lays the groundwork for the development of new microporous materials.

      • 2차원 및 3차원 초음파를 이용한 태아표면의 해부학적 선별 검사에 관한 연구

        윤석범,이해혁,이정재,최경훈,이순곤,이임순,이권해 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.2

        Objective : Our purpose of this study was to determine the clarity and consuming time of fetal surface screening in thirty-eight fetuses from 24 to 32 weeks of gestation by use of two and three-dimensional ultrasonography. Methods : From May 1998 to July 1998, we performed the fetal surface screening of the fetal face, extremities, and spine. Thirty-eight fetuses of 24-32 weeks of gestation (thirty-seven normal fetuses and one abnormal fetus) were studied by use two-dimensional (AI 5200S, 3.5-5.0MHz; Acoustic Imaging Technologic Cooperation, U.S.A.) and three-dimensional ultrasonography (Volusion 530D, 3.5-5.0 MHz ; Kretz-Medicine, Korea). On the fetal face, we evaluated both orbits, nose, lips and brow. Both hands and feet, five digits and toes should be seen in this study. We evaluated cervical spine and lumbosacral junction of the spine. Results : The maternal average age of 38 patients was 29.9 years and the average gestational age was 29.7 weeks. The results of fetal surface screening were as follows : face (both orbits 89.5%, lips 92.1%, nose 92.1%, and brow 89.5%), extremities (both hands 68.4% and feet 73.7%), and spine (cervical spine 94.7% and lumbosacral junction 97.4%) by use of two-dimensional ultrasonography. By use of three-dimensional ultrasonography, results were as follows : face (both orbits 76.3%, lips 81.6%, nose 81.6%, and brow 78.9%), extremities (both hands 76.3% and feet 78.9%), and spine (cervical spine 71.1% and lumbosacral junction 73.7%). Consuming time for two-dimensional ultrasonography were 4.7 minutes for face, 11.6 minutes for extremities, and 5.7 minutes for spine. Using three-dimensional ultrasonography results were 12.8 minutes for face, 17.9 minutes for extremities, and 11.7 minutes for spine. Average consuming time of all fetal anatomic screening was 22.0 and 42.4 minutes by use of two and three dimensional ultrasonography. In one abnormal fetus had cleft lip and polydactyly of right foot which were detected all by three-dimensional ultrasonography, but we couldn't detect the polydactyly by two-dimensional ultrasonography. Conclusion : Three-dimensional ultrasonography was more time-consuming method, but we suggested that three-dimensional ultrasonography was superior than two-dimensional ultrasonography in fetal surface screening.

      • KCI등재

        무균돈사내 급배기구의 적정위치설정에 관한 연구

        권순정,손덕영,최윤호 한국의료복지시설학회 2008 의료·복지 건축 Vol.14 No.4

        Construction and operation of Germ Free Pigs' facilities are very expensive because pigs' rooms and other major rooms of the facility require germfree environments. Especially, running the HVAC system of aseptic facilities requires a lot of expenses. However proper location and efficient shape of outlets/inlets for the ventilation of the room can reduce the excessive running cost. In order to do that, this study proposes alternative location and shape of ventilation outlets/inlets to the existing design pattern in germfree pigs' room. The design condition of this study is the maintenance of adequate temperature(24'C), NHs concentration level(below 1.5 ppm), and air stream speed(be1ow .25m/sec) in the pigs' room for the summer and the winter together. As the Software Program, FLUENT(Ver. 6.2) has been used for the analysis of proposed ventilation patterns, In conclusion, wall inlets and ceiling inlet/outlet are advisable in summer, wall inlets and ceiling outlets is advisable in winter. As far as the shape is concerned, diffuser type for the ceiling outlet is desirable.

      • 한국인에서의 CYP2E1 유전자형

        권준택,김형기,손동렬,염윤기 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

        Cytochrome P4502E1 (CYP2E1) is inducible by ethyl alcohol and activates procarcinogenic N-nitrosodimethylamine (NDMA), benzene, urethane and other lower molecular weight compound. CYP2E1 is also involved in metabolism of certain drugs, for example, alcohol, acetaminophen, tamoxifen, theophylline, fluorinated anesthetics, and chlorozoxazone. CYP2E1 activity was shown to be polymorphically distributed in humans and has been suggested to play a role in hepatocellular carcinoma and alcoholic related disorders. Although genetic predisposition to alcoholism and alcoholic liver disease has been reported, genetic susceptibility to alcoholic pancreatitis is still a matter of debate. The aims of this study were to investigate the allelic frequency of CYP2E1 Rsa I polymorphism in Koreans. We investigated the frequency distribution of CYP2E1 Rsa I polymorphism in 212 unrelated healthy Koreans and 42 hepatocellular carcinoma patients. Detection of the CYP2E1 alleles was performed by polymerase chain reaction and restriction fragment length analysis. The genomic DNA was isolated from peripheral blood with conventional phenol: chloroform extraction method. The allelic frequencies of c1 and c2 in healthy volunteers were 0.85 and 0.15, respectively. The rare type (c2/c2) of CYP2E1 Rsa I polymorphism was 0.005 in healthy volunteers.

      • 제지 폐수의 질소·인산 동시 제거를 위한 생물학적 처리기술 개발

        권기석,노윤숙 안동대학교 환경연구소 2001 환경연구 Vol.1 No.-

        주변에서 원유로 오염된 토양과 난분해성 화합물을 포함하고 있는 폐수 등에서 균주를 분리하여 40여종의 분해균주 중 가장 분해력이 우수한 분해균주 4종을 LB(Luria Bertani)배지로 최종분리하였다. 자연계의 균원 시료로부터 분리된 균주 중 점성을 갖는 균주를 선별하여 그 중 표준 점토광물인 kaolin clay에 대한 응집활성이 뛰어난 34종류이 균주를 선택하였으며, 폐수 내에서 응집활성이 높은 응집균주 4종을 최종 분리하였다. 균주 #46(Bacillus circulans), O24(Pseudomonas sp.)2종을 제외한 나머지 균주들의 동정결과는 #24T3, #24P3, #24B3는 Bacillus sp.로 동정되었고 #81, #152, 29은 미동정 되었다. 분해균주의 경우 TOC가 84%, T-N이 98%, T-P가 68%의 처리효율을 보였다. 응집균주의 경우 TOC가 80%, T-N 98%, T-P가 71%의 처리효율을 보였다. 분해+응집균주가 microcosom을 형성한 경우 TOC가 85%, T-N의 경우 98%이상, T-P의 경우 70%을 동시에 처리할 수 있다. From the enrichment culture, 40 strains were isolated, Among them, four strains were screend as having significant biodegradation activity of crude oil and toxic compounds containing wastewater. Also, for the screening of the microorganims, 34 bacterial strains were selected. Among them, four strains were screened as producing the most excellent flocculating material. The strains were identified that 46 was Bacillus circulates, 024 was Pseudomonas sp., 24T3, 24B3, 24P3 were identified Bacillus sp., respectively, And 81, 151, 29 were non-identification. The optimum cultural conditions were as follows; Bioflocculant producing strains : Glucose 20.0g, NH_(4)NO_(3) 2.0g, K_(2)HPO_(4) 0.8g, KH_(2)PO_(4) 0.6g, MgSO_(4)·7H_(2)O 0.05g, MnSO_(4)·4H_(2)O 0.05g, Soytone 0.lg, Tryptone 0.lg Yeast extract 0.lg, CaCO_(3) 0.3g in 1 liter of distilled water, 30℃ for temperature and pH 7.0. Biodegrade strains : LB(Luria Bertani) medium, 30℃ for temperature and pH 6.8∼7.5. The removal rates of total nitrogen and total phosphate by bioflocculant producing strains against pulp wastewater were T-N 98% and T-P 71%, respectively. In the case of biodegrade strains shown that the removal rates were T-N 98% and T-P 68%, respectively. In the case of mixed biodegrade strains and bioflocculant producing strains shown that the removal rates were T-N 99% and T-P 76%, respectively. The strains will be apply simultaneously T-N 98% and T-P 70% from pulp waserwater.

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