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( Qiuke Hou ),( Yongquan Huang ),( Changrong Zhang ),( Shuilian Zhu ),( Peiwu Li ),( Xinlin Chen ),( Zhengkun Hou ),( Fengbin Liu ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2018 Journal of Neurogastroenterology and Motility (JNM Vol.24 No.4
Background/Aims MicroRNAs (miRNAs) were reported to be responsible for intestinal permeability in diarrhea-predominant irritable bowel syndrome (IBS-D) rats in our previous study. However, whether and how miRNAs regulate visceral hypersensitivity in IBS-D remains largely unknown. Methods We established the IBS-D rat model and evaluated it using the nociceptive visceral hypersensitivity test, myeloperoxidase activity assay, restraint stress-induced defecation, and electromyographic (EMG) activity. The distal colon was subjected to miRNA microarray analysis followed by isolation and culture of colonic epithelial cells (CECs). Bioinformatic analysis and further experiments, including dual luciferase assays, quantitative real-time polymerase chain reaction, western blot, and enzyme-linked immunosorbent assay, were used to detect the expression of miRNAs and how it regulates visceral hypersensitivity in IBS-D rats. Results The IBS-D rat model was successfully established. A total of 24 miRNAs were differentially expressed in the distal colon of IBS-D rats; 9 were upregulated and 15 were downregulated. Among them, the most significant upregulation was miR-200a, accompanied by downregulation of cannabinoid receptor 1 (CNR1) and serotonin transporter (SERT). MiR-200a mimic markedly inhibited the expression of CNR1/SERT. Bioinformatic analysis and luciferase assay confirmed that CNR1/SERT are direct targets of miR-200a. Rescue experiments that overexpressed CNR1/SERT significantly abolished the inhibitory effect of miR-200a on the IBS-D rats CECs. Conclusions This study suggests that miR-200a could induce visceral hyperalgesia by targeting the downregulation of CNR1 and SERT, aggravating or leading to the development and progression of IBS-D. MiR-200a may be a regulator of visceral hypersensitivity, which provides potential targets for the treatment of IBS-D. (J Neurogastroenterol Motil 2018;24:656-668)
( Qiuke Hou ),( Yongquan Huang ),( Yan Wang ),( Liu Liao ),( Zhaoyang Zhu ),( Wenjie Zhang ),( Yongshang Liu ),( Peiwu Li ),( Xinlin Chen ),( Fengbin Liu ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.10
Our previous report determined that miR-144 is a key regulator of intestinal epithelial permeability in irritable bowel syndrome with diarrhea (IBS-D) rats. Recent evidence has shown that lactobacilli play an important role in the relief of IBS-D symptoms. However, few studies have addressed the mechanisms by which microRNAs and lactobacilli exert their beneficial effects on intestinal epithelial permeability. Hence, to elucidate whether miRNAs and lactobacilli play roles in intestinal epithelial barrier regulation, we compared miRNA expression levels in intestinal epithelial cells (IECs) under Lactobacillus casei (L. casei LC01) treatment. IECs and L. casei LC01 were co-cultured and then subjected to microRNA microarray assay. qRT-PCR, western blot and ELISA were used to detect the expression of occludin (OCLN) and zonula occludens 1 (ZO1/TJP1). The interaction between miRNAs and L. casei LC01 acting in IECs was investigated through transfection of RNA oligoribonucleotides and pcDNA 3.1 plasmid. The results are as follows: 1) L. casei LC01 decreased the expression of miR-144 and FD4 and promoted OCLN and ZO1 expression in IECs; 2) L. casei LC01 enhanced the barrier function of IECs via downregulation of miR-144 and upregulation of OCLN and ZO1; 3) Under L. casei LC01 treatment, OCLN and ZO1 overexpression could partially eliminate the promoting effect of miR-144 on intestinal permeability in IECs. Our results demonstrate that L. casei LC01 regulates intestinal permeability of IECs through miR-144 targeting of OCLN and ZO1. L. casei LC01 can be a possible therapeutic target for managing dysfunction of the intestinal epithelial barrier.
Qiang Xu,Shengxiang Lei,Yongquan Zhu,Wei Zhao,Cong Wang,Dapeng Wang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.9
The deformation pattern of the stratum caused by constructing a new metro tunnel crossing an existing tunnel is different from the deformation pattern caused by general tunnel construction. However, the prediction results by the ordinary surface settlement prediction model often bring significant errors because the complex influence of existing tunnels on the surface settlement caused by the excavation of new tunnels is always neglected. Based on the equivalent layered method and stochastic medium theory, a prediction model for the surface settlement due to excavating a new tunnel under an existing tunnel in the heterogeneous stratum was established. By equating the bending stiffness of an existing tunnel before and after applying the equivalent layered method, the layer index was determined. The critical parameters of the stochastic medium theory were derived based on the relationship between the critical parameters of both the Peck empirical formula and the stochastic medium theory. The surface settlement of some typical projects was predicted and compared by the prediction model in this paper and the ordinary prediction model. The comparison shows that the proposed prediction model and parameter determination method in this paper had high accuracy and applicability. The results of the prediction model in this paper fit the results of numerical calculation. The research of this paper can provide a new method for the theoretical prediction of surface settlement caused by the excavation of a new tunnel under an existing tunnel in the heterogeneous stratum and the determination of critical parameters of the stochastic medium theory.problem in the construction industry, and helps reducing the material waste and budget cost.
Isobaric vapor-liquid equilibrium for methanol+benzene+1-octyl-3-methylimidazolium tetrafluoroborate
Qunsheng Li,Baohua Wang,Wei Zhu,Yongquan Fu,Magtuf Zuhir,Lun Li 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.7
Isobaric vapor-liquid equilibrium (VLE) data for {methanol (1)+benzene (2)+1-octyl-3-methylimidazolium tetrafluoroborate (3)} where 3 is an ionic liquid ([OMIM]+[BF4]−) at atmospheric pressure (101.32 kPa) were measured with a modified Othmer still. The results showed that the ionic liquid studied can transfer the azeotropic point and eliminate the azeotropic phenomena when its concentration is up to x3=0.30. This means that [OMIM]+[BF4]− can be used as a promising entrainer in the application of extractive distillation. The measured ternary data were correlated using the NRTL model.
Tushuai Li,Li Chen,Xiao Fu,Zhihong Liu,Shenglong Zhu,Yongquan Chen,Jie Zhang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.112 No.-
Chemodynamic therapy (CDT) based on multifunctional nanoparticles (NPs) has occurred as an attractivecancer treatment that covers the shortage of nonnegligible limitations from single therapy. Herein, wesynthesize a safe and universal CDT nanoparticle—iron single-atom nanoparticle (Fe SANP), in whichFe single-atom as the active catalytic site is anchored in a carbon framework with encapsulated doxorubicin(DOX). Fe SANPs work as catalase-like nanozyme for hydroxyl radicals (OH) production. The preparedNPs (denoted as DOX@Fe SANPs) can efficiently mediate peroxidase-like activity with the existenceof H2O2 that enhances cancer cell elimination by generating abundant OH. DOX@Fe SANPs exhibit a pHinduceddegradable character that contributes to specific drug release in the tumor microenvironment(TME). DOX@Fe SANPs produce ignorable systematic toxicity after biodegradation and drug delivery processes. Collectively, this study highlights the efficient anti-tumor performances of the iron single-atomnanocatalysts containing an anti-cancer drug DOX.