Genome-wide transcriptome analysis of rice genes responsive to chilling stress

Cho, Hyun Yong,Hwang, Sun-Goo,Kim, Dong Sub,Jang, Cheol Seong Canadian Science Publishing 2012 Canadian journal of plant science. Revue canadienn Vol.92 No.3

<P> Cho, H. Y., Hwang, S.-G., Kim, D. S. and Jang, C. S. 2012. Genome-wide transcriptome analysis of rice genes responsive to chilling stress. Can. J. Plant Sci. 92: 447-460. Low temperature is known to be one of the major challenges to rice production. We have selected chilling-tolerant TILLING of Donganbyeo, which showed significantly vigorous growth relative to wild-type plants under chilling stress conditions (10-12°C). We performed a comparative transcriptome analysis using a chilling-tolerant line and wild-type plant with the objective to evaluate genomic responses to chilling stress and to identify chilling inducible genes. Functional enrichment analysis results demonstrated that a large proportion of chilling-inducible genes were associated with certain biological pathways, e.g., monosaccharide catabolic processes, reflecting the energy requirements necessary for adaptation to sub-optimal temperatures in plants. Extremely low correlation coefficients in a range of −0.07 to 0.04 were detected between plant responses to chilling stress and different abiotic stress conditions such as drought, salt, cold, and heat; these results imply that plants might exploit strikingly different response mechanisms against stress conditions. The largest subnetwork, which was composed of 78 chilling-specific inducible genes, was found in the tolerant plants, but not in the wild-type plants, which probably implies the existence of a delicate and harmonious signaling pathway setup in the tolerant plants. Expression patterns of 20 chilling-responsive genes were assessed via abiotic stress treatments and phytohormone treatments. About 80% of the tested chilling-inducible genes were upregulated by exogenous abscisic acid (ABA) treatment. The results of this study may prove useful in elucidating the chilling-response pathway and in the development of chilling-tolerant rice varieties. </P>

Acute and Sub-chronic Oral Toxicity Study of Ammonium Persulfate in Spraque-Dawley Rats

Yong Soon Kim,Min Won Baek,Jae Hyuck Sung,Hyun Youl Ryu,Jin Sik Kim,Hyun Sun Cho,Byung Gil Choi,Min Sub Song,Moon Yong Song,Eun Ju Baik,Young Kuk Choi,Jong Kyu Kim,Il Je Yu,Kyung Seuk Song 한국독성학회 2009 Toxicological Research Vol.25 No.3

The toxicity test of ammonium persulfate was conducted to ensure of its potential toxic effects according to the single-dose acute oral toxicity study (OECD Guideline 423) and 90-day repeated dose sub-chronic oral toxicity study guideline (OECD Guideline 408) for establishing national chemical management system, and matching in the Globally Harmonized Classification System (GHS) category. In acute oral toxicity study, pasty stool, perineal contamination and temporary body weight decrease were observed after dosing 1st and 2nd challenge (300 ㎎/㎏ body weight). All test animals were dead within 6 hours after dosing at 3rd challenge (2000 ㎎/㎏ body weight). Therefore, the GHS class of test substance is considered class 4. In sub-chronic toxicity study, body weight changes, food consumptions, hematological, biochemical and pathological examination did not show any noticeable and significant differences between the administered (5, 20, 80 ㎎/㎏ body weight) and control (vehicle only) group animals. Based on these results, the no observed adverse effect level (NOAEL) is considered above 80 mg/kg body weight.

Acute and Sub-chronic Oral Toxicity Study of Ammonium Persulfate in Spraque-Dawley Rats

Kim, Yong-Soon,Baek, Min-Won,Sung, Jae-Hyuck,Ryu, Hyun-Youl,Kim, Jin-Sik,Cho, Hyun-Sun,Choi, Byung-Gil,Song, Min-Sub,Song, Moon-Yong,Baik, Eun-Ju,Choi, Young-Kuk,Kim, Jong-Kyu,Yu, Il-Je,Song, Kyung-Se Korean Society of ToxicologyKorea Environmental Mu 2009 Toxicological Research Vol.26 No.3

The toxicity test of ammonium persulfate was conducted to ensure of its potential toxic effects according to the single-dose acute oral toxicity study (OECD Guideline 423) and 90-day repeated dose sub-chronic oral toxicity study guideline (OECD Guideline 408) for establishing national chemical management system, and matching in the Globally Harmonized Classification System (GHS) category. In acute oral toxicity study, pasty stool, perineal contamination and temporary body weight decrease were observed after dosing 1st and 2nd challenge (300 mg/kg body weight). All test animals were dead within 6 hours after dosing at 3rd challenge (2000 mg/kg body weight). Therefore, the GHS class of test substance is considered class 4. In sub-chronic toxicity study, body weight changes, food consumptions, hematological, biochemical and pathological examination did not show any noticeable and significant differences between the administered (5, 20, 80 mg/kg body weight) and control (vehicle only) group animals. Based on these results, the no observed adverse effect level (NOAEL) is considered above 80 mg/kg body weight.

대기압 스트리머 코로나방전특성 및 이산화탄소 전환특성

조문수,김학규,곽동주,신용섭 경성대학교 공학기술연구소 2001 공학기술연구지 Vol.8 No.-

Deep interests have been paid on the application of non-thermal plasma technique th solve the environmental pollution problems. CO_2 is one of the severe pollutants which cause the acid rain and global warming. In this study, in order to improve the conversion efficiency of CO_2, the streamer corona discharge plasma and barrier discharge plasma reactors were made, and the conversion characteristics of CO_2 by the corona discharge plasma and some discharge characteristics of these discharge chambers are studied experimentally.

Polysulfone 블럭 공중합체를 이용한 음이온 교환막의 제막 및 전기화학적 특성

황용구,조경섭,강안수 明知大學校 産業技術硏究所 2000 産業技術硏究所論文集 Vol.19 No.-

It was prepared an anion exchange membrane using block copolymer of Polysulfone(PSf) and polyphenylene sulfide sulfone(PPSS) by a two step synthesis: the chloromethylation and amination by triethylamine, and studied the effect of the preparation condition on the electrochemical properties such as the electrical area resistance, the ion exchange capacity, and the static transport number. The optimum chloromethylation condition were mole ratio of BPSf to chloromethyl methyl ether 1:20, reaction time 6 hours, and reaction temperature 25℃. The best electrochemical properties were measured, with area resistance 120.9 Ω·㎠, ion exchange capacity 1.324 meq./ g dry membrane, water content 0.204 g H₂O/g dry membrane, and fixed ion concentration 6.49 meq./g H₂O.

응급실을 통한 정신과 입원환자에 대한 임상적 고찰

소용섭,정영조,오석환 大韓神經精神醫學會 1988 신경정신의학 Vol.27 No.2

Hospital records of 127 neuropsychiatric patients admitted to Seoul Paik Hospital via the E.R. from January 1, 1984 to December 31, 1986 were reviewed, and the following results were obtained. 1) Patients admitted via the E.R. comprised 22.8% of total neuropsychiatric admissions. 2) The readmission rate of patients via E.R. was 36.2%, that of the patients admitted via the O.P.D. was 22.7% 3) The average duration of hospitalization of neuropsychiatric pateints admitted via the E.R. was 36 days that of the patients admitted via the O.P.D. was 48.3 days. 4) 32.3% of the E.R. admission patients were in their twenties ; 55.9% of them were in their twenties or thirties. Male for female ratio was 1.15 : 1 5) 22.8% of the E.R. patients were diagnosed as schizophrenic disorder ; which was the most frequent diagnosis in all patients. 6) 34.6% of the E.R. patients were discharged within 2 weeks, and 60.6%, within 1 month. 7) Presenting problems, in the order of frequency, were paralysis of numbness of extremities, somatic sysmptoms, hallucination of paranoid delusion, and anxiety or panic state. More than half of the patients presented one or more of these symptoms. 8) 36.2% of the E.R. patients were discharged against medical advice. This was higher than A.M.A. discharge rate of 26.7% of the patients admitted via the O.P.D.

Study on production process of graphite for biological applications of 14C-accelerator mass spectrometry

하영수,김계령,Yong-Sub Cho,최규민,Chaewon Kang 대한방사성의약품학회 2020 Journal of radiopharmaceuticals and molecular prob Vol.6 No.1

Accelerator mass spectrometry (AMS) is a powerful detection technique with the exquisite sensitivity and high precision compared with other traditional analytical techniques. Accelerator mass spectrometry can be widely applied in the technique of radiocarbon dating in the fields of archeology, geology and oceanography. The ability of accelerator mass spectrometry to measure rare 14C concentrations in microgram and even sub-microgram amounts suggests that extension of 14C-accelerator mass spectrometry to biomedical field is a natural and attractive application of the technology. Drug development processes are costly, risky, and time consuming. However, the use of 14C-accelerator mass spectrometry allows absorption, distribution, metabolism and excretion (ADME) studies easier to understand pharmacokinetics of drug candidates. Over the last few decades, accelerator mass spectrometry and its applications to preclinical/clinical trials have significantly increased. For accelerator mass spectrometry analysis of biological samples, graphitization processes of samples are important. In this paper, we present a detailed sample preparation procedure to apply to graphitization of biological samples for accelerator mass spectrometry.

Study on production process of graphite for biological applications of ¹⁴C-accelerator mass spectrometry

Ha, Yeong Su,Kim, Kye-Ryung,Cho, Yong-Sub,Choe, Kyumin,Kang, Chaewon 대한방사성의약품학회 2020 Journal of radiopharmaceuticals and molecular prob Vol.6 No.1

Accelerator mass spectrometry (AMS) is a powerful detection technique with the exquisite sensitivity and high precision compared with other traditional analytical techniques. Accelerator mass spectrometry can be widely applied in the technique of radiocarbon dating in the fields of archeology, geology and oceanography. The ability of accelerator mass spectrometry to measure rare ¹⁴C concentrations in microgram and even sub-microgram amounts suggests that extension of ¹⁴C-accelerator mass spectrometry to biomedical field is a natural and attractive application of the technology. Drug development processes are costly, risky, and time consuming. However, the use of ¹⁴C-accelerator mass spectrometry allows absorption, distribution, metabolism and excretion (ADME) studies easier to understand pharmacokinetics of drug candidates. Over the last few decades, accelerator mass spectrometry and its applications to preclinical/clinical trials have significantly increased. For accelerator mass spectrometry analysis of biological samples, graphitization processes of samples are important. In this paper, we present a detailed sample preparation procedure to apply to graphitization of biological samples for accelerator mass spectrometry.

Anti-Cancer Effect of Ginsenoside F₂ against Glioblastoma Multiforme in Xenograft Model in SD Rats

Ji Yon Shin,Jung Min Lee,Heon Sub Shin,Sang Yong Park,Jung Eun Yang,Somi Kim Cho,Tae-Hoo Yi 고려인삼학회 2012 Journal of Ginseng Research Vol.36 No.1

The glioblastoma multiforme (GBM) is the most common malignant brain tumor in adults. Despite combination treatments of radiation and chemotherapy, the survival periods are very short. Therefore, this study was conducted to assess the potential of ginsenoside F₂ (F2) to treat GBM. In in vitro experiments with glioblastoma cells U373MG, F2 showed the cytotoxic effect with IC?? of 50 ㎍/mL through apoptosis, confi rmed by DNA condensation and fragmentation. The cell population of cell cycle sub-G1 as indicative of apoptosis was also increased. In xenograft model in SD rats, F2 at dosage of 35 ㎎/㎏ weight was intravenously injected every two days. This reduced the tumor growth in magnetic resonance imaging images. The immunohistochemistry revealed that the anticancer activity might be mediated through inhibition of proliferation judged by Ki67 and apoptosis induced by activation of caspase-3 and -8. And the lowered expression of CD31 showed the reduction in blood vessel densities. The expression of matrix metalloproteinase-9 for invasion of cancer was also inhibited. The cell populations with cancer stem cell markers of CD133 and nestin were reduced. The results of this study suggested that F2 could be a new potential chemotherapeutic drug for GBM treatment by inhibiting the growth and invasion of cancer.

SnO₂-Coated 3D Etched Cu Foam for Lithium-ion Battery Anode

Um, Ji Hyun,Kim, Hyunwoo,Cho, Yong-Hun,Yoon, Won-Sub The Korean Electrochemical Society 2020 Journal of electrochemical science and technology Vol.11 No.1

SnO₂-based high-capacity anode materials are attractive candidate for the next-generation high-performance lithium-ion batteries since the theoretical capacity of SnO₂ can be ideally extended from 781 to 1494 mAh g^-1. Here 3D etched Cu foam is applied as a current collector for electron path and simultaneously a substrate for the SnO₂ coating, for developing an integrated electrode structure. We fabricate the 3D etched Cu foam through an auto-catalytic electroless plating method, and then coat the SnO₂ onto the self-supporting substrate through a simple sol-gel method. The catalytic dissolution of Cu metal makes secondary pores of both several micrometers and several tens of micrometers at the surface of Cu foam strut, besides main channel-like interconnected pores. Especially, the additional surface pores on etched Cu foam are intended for penetrating the individual strut of Cu foam, and thereby increasing the surface area for SnO₂ coating by using even the internal of Cu foam. The increased areal capacity with high structural integrity upon cycling is demonstrated in the SnO₂-coated 3D etched Cu foam. This study not only prepares the etched Cu foam using the spontaneous chemical reactions but also demonstrates the potential for electroless plating method about surface modification on various metal substrates.